African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007

AJMR Articles in press

Article in Press

First Novel Phages from Rodents with Lytic Activity on Clinical Enterobacteriaceae strains: Initiation for Phage Therapy in West Africa

Bacteriophages (phages) are viruses that infect bacteria. The emergence of resistant bacteria has been reported worldwide. The use of phages to treat bacterial infection or to reduce bacteria load has become a new area of interest. The viral colonization of rodents represents an important source for a specific biotope. The objective of this study was to characterize the effect of novel phages isolated from rodents...

Author(s):Kakou-Ngazoa E. Solange, Addablah A. Audrey, Krylova Katerina, Saraka Daniel, Kouassi K. Stéphane, Coulibaly Ngolo David, Sina Kouamé Mireille, Aoussi Serge, Dozois Charles and Dosso Mireille

Article in Press

Antibiogram studies of Escherichia coli and Salmonella species isolated from diarrheal patients attending Malam Mande general hospital Dutsin-Ma, Katsina State, Nigeria

Antibiotics resistant bacteria (ARB) is a worldwide problem. Information on ARB associated with diarrheal stool samples from Dutsin-Ma, Katsina State, Nigeria is scare. This study examines 41 stool samples from diarrhea patients from a selected hospital in Dutsin-Ma. Questionnaires were used to collect demographic information and used antibiotics. Bacteria isolation and antibiotics susceptibility testing were determined...

Author(s):Ayodele Timilehin Adesoji and Ahmad Mansur Liadi

Article in Press

Studies on Antiretroviral Treatment Failure among HIV Positive Clients Attending Aminu Kano Teaching Hospital (AKTH), Kano.

Antiretroviral (ART) treatment failure is a major obstacle in the management of HIV positive clients. This study was carried out to determine the prevalence of ART treatment failure among HIV positive clients attending Aminu Kano Teaching Hospital. The study was conducted by assessing immunological (CD4 cells count) and virological (viral load test) parameters. Of the 120 HIV positive clients tested, 27 (22.5%) had...

Author(s):USMAN DUTSINMA ALIYU, Bello Ahmed Salim

Article in Press

Isolation and identification of nitrite-degrading lactic acid bacteria from traditional Sichuan pickle

Sichuan traditional pickle is fermented by pickling with aged brine which is used for years even decades. The objective of this study was to isolate and identify autochthonous nitrite-degrading lactic acid bacteria from traditional Sichuan fermented radish. Three lactic acid bacteria strains with high nitrite-degrading ability were screened and identified as Leuoconostoc mesenteroieds (R5) and Weissella sp (R2, R4) by...

Author(s):SHUANGSHUANG GUO, Yan Ge, Anjun Chen

Article in Press

PREVALENCE AND ANTIBIOGRAM OF LISTERIA MONOCYTOGENES IN YOGHURT AND RAW MILK SAMPLES IN ZARIA, KADUNA STATE, NIGERIA

Listeria monocytogenes is a food borne pathogen frequently associated with the consumption of contaminated food. Severe and life threatening human diseases caused by Listeria monocytogenes has been reported throughout the world. The present study evaluated the prevalence and antibiograms of Listeria monocytogenes isolated from yoghurt and raw milk sample in Zaria, Kaduna State. A total of three hundred and sixteen samples...

Author(s):Veronica C.

Article in Press

Antibiotics Susceptibility Profile and Killing Rate of Cronobacter sakazakii Isolated fro Used, Retail Infant Formulas and Opened-Vended Milk in Nigeria

Cronobacter sakazakii was isolated from Used and Retail Powdered Infant Formulas (PIF) and Opened-vended Powdered Milk (OVPM) samples using standard methods comprising Enterobacteriaceae Enrichment Broth and HARDYCHROM sakazakii medium. The antibiotics susceptibility results showed that out of the forty-four (44) of the Cronobacter sakazakii isolates tested, 44 (100%) was sensitive to Ciprofloxacin, 27 (61.36%) to...

Author(s):STEPHEN DARE OLONINEFA, Safiya Yahaya Daniyan, Moses Enemaduku Abalaka

Article in Press

Management of Powdery Mildew, Alternaria and Bacterial blight complex of Cluster Bean under Organic Farming System

Organic farming system emphasis on sustainable development of agriculture, the traditional agriculture system was much akin to the organic system but modernization of agriculture made a shift to this trend. Field experiments were conducted during kharif (Rain fed) 2013 and 2014 to develop a suitable organic module to manage these diseases and improve productivity of cluster bean. The module consisting of soil application...

Author(s):HARSHRAJ KANWAR, Amit Trivedi

Article in Press

Purification of a Saponin with antifungal activity isolated from the seeds of Filicium decipiens plant: Hemolytic inhibition activity by the Lectin PpyLL

Triterpenoids are a large group of naturally occurring substances in plant kingdon. In this paper was presented a Saponin from Fillicium decipiens seeds purified through a protocol using Ion-exchange coupled-chromatography for protein retention followed by Gel filtration under Sephadex-LH-20, and characterized by HPLC-MS. Saponin was examined for its antifungal properties by sensibility and dilution methods, and submitted...

Author(s):BRANDO-COSTA ROMERO, Ferreira Araújo Vivianne

Article in Press

Multiplex PCR technique for rapid detection of indicator bacteria in drinking water

Microbial indicators, in particular coliform bacteria including Escherichia coli and Enterococci have been established for detecting fecal contamination in water. These indicators, used to assess the potential public health risk of drinking water and their presence or absence, are key elements of most drinking water quality guidelines. Approved traditional methods for indicator organisms detection include the multiple-tube...

Author(s):El-Sayed Ahmed Kassem A.

Article in Press

DETERMINATION GENOYTYPE D OF HEPATITIS B VIRUSAMONGST PATEINTS IN MOSUL-IRAQ

Background: Hepatitis means inflammation of liver, caused by several viruses and agents. Hepatitis B virus is one of many types of viruses can infect liver and causes inflammation and in sometimes reaching to cirrhosis and hepatocellular carcinoma. Objectives: the aim of this study is to detect the genotype of hepatitis B virus from patients in Mosul. Methods: We evaluate 25 patients presumably with HBV in acute and...

Author(s):ALI ADEL DAWOOD

Article in Press

SOKOTO ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH UROGENITAL TRACTS OF FEMALE PATIENTS ATTENDING SOME SELECTED HOSPITALS WITHIN METROPOLIS

The research was aimed at Isolation and Identification of Anaerobic and Aerobic bacteria present in theUrogenital tracts of female patients attending Specialist Hospital Sokoto and Maryam Abacha Women and Children Hospital Sokoto. A total of forty (40) samples were collected from 40 female patients. A total of twenty (20) High vagina swab (HVS) samples and twenty(20) urine samples were collected. Bacteria isolated were...

Author(s):SA'ADATU ALIYU SHINKAFI

Article in Press

In vitro antibacterial activity of an endemic plant of south Algeria

Resistances to current antibacterial drugs are growing global concerns. Traditional medicine is a potential source of new antibacterial. The main purpose of this study firstly, it is to find the chemical compounds in the Citrullus colocynthis Schard fruits leaves and roots , secondly, to evaluate the antibacterial activity of aqueous and hydromethanolic extract of the different parts. Phytochemical screening revealed the...

Author(s):Nora Nahal

Article in Press

Studied on the Antibacterial Potential of Actinomycetes Isolated from the Haridwar Region of Uttarakhand

In this study, 29 isolates of actinomycetes were isolated from soil samples collected in the area of various localities of Haridwar region. These isolates were tested for their antagonistic properties against test organisms Bacillus subtilis, Micrococcus luteus, Staphylococcus epidermis, Pseudomonas aeruginosa, Serratia marcesens, and Escherichia coli. Some of these actinomycetous strains exhibited antimicrobial activity...

Author(s):GARIMA ARYA, Purshotam Kaushik

Article in Press

Distribution Pattern and Molecular Evaluation of Facultative Anaerobic Thermophilic Bacteria in Hot Spring Sehezar, Iran

Generally, hot areas such as hot springs have high potential for growth of thermophilic bacteria. These bacteria are very useful in various industries in the fields of microbiology and biotechnology. One of the most important and most diverse groups of thermophiles is Bacillaceae family. Many species of this family are Extremophiles. Recently, scientists have found a tendency to study about thermophilic bacteria, thus they...

Author(s):Seyed Davar Siadat

Article in Press

Production of Lovastatin by Monascus purpureus MTCC369 using corn bran under solid state fermentation

Production of lovastatin was carried out by Monascuspurpureus MTCC 369 using agro-industrial residues likecorn bran as substrate under solid state fermentation. Lovastatin is a potent hypercholesterolemic drug used forlowering blood cholesterol. In recent years, lovastatin has also been reported as a potential therapeutic agent forthe treatment of various types of tumors and also play a tremendous role in the regulation of...

Author(s):PRASANNA LATHA

Article in Press

In Vitro Detection of Antibacterial Activity of Glycyrrhizic Acid Nanoparticle against Multi-Drug Resistant K. pneumoniae with Genotypic Analysis

Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae can resist many antibiotic groups as they contain resistant genes responsible for multi-drug resistance. the study aimed to detect and identifying multi-drug resistant (MDR) K. pneumoniae from different clinical samples with detection and sequencing of both TEM and SHV genes, and using Glycyrrhizic acid nanoparticle as a treatment agent for MDR, ESBL...

Author(s):HAMDY AHMED EL-KHATEEB, Mahmoud Abd El-Mongy, Amal Sabry Othman

Article in Press

Microbial analysis of drinking water from rawalakot muncipality of Azad Jammu And Kashmir

Water has always been an important and life-sustaining drink to humans and is essential for the survival of all organisms. Therefore, a study was carried out to analyze the safety of drinking water. Samples of water will be collected from different sources available in Rawalakot municipality of Azad Jammu and Kashmir and were analyzed for microbiological parameters. Microbiological parameters include total coli forms,...

Author(s):KHAN

Article in Press

Microbiological Safety of Raw Cow Milk across the Milk Supply Chain in Eastern Ethiopia

This study was conducted to determine the microbiological safety of informally marketed raw cow milk across the milk supply chain in eastern Ethiopia. A total of 360 pooled raw cow milk samples (each with a volume of 450 mL) were collected from the udders and milk handling equipment of producers in rural areas of Babile district; from the equipment of collectors/transporters in Harar and Dire Dawa towns as well as from the...

Author(s):Tadele Amentie

Article in Press

Isolation and Identification of Fungi from soil samples in Amurum Forest Reserve

The Amurum forest reserve is an area of about 300 hectares that is protected because it houses important bird species. This study was carried out to investigate the fungal diversity in soil samples collected from three Habitat types present in Amurum Forest Reserve. The fungi were isolated using soil plate method, subcultured to obtain pure cultures and maintained on PDA slants. On the basis of cultural and microscopic...

Author(s):Diana Unwana Affiah, Charity Ada Amienyo, and Vou Moses Shutt

Article in Press

Presence of phytopathogenic fungi and oomycetes on rice and avocado crops in Tolima (Colombia).

Tolima is one of the largest zones in Colombia were avocado and rice are produced. The disease management programs for these crops are mainly chemical-dependent and tend to have variable results since the diagnosis of some diseases is based on unspecific symptoms. In this context, the present study aimed to bring information about the phytopathogens affecting Tolima´s rice and avocado crops. From foliar tissue, culm, and...

Author(s):Vinchira-Villarraga Diana Marcela, Macias-Camacho Jeimy, Mendez-Olivera Juan David, Méndez-Tibambre María Elizabeth, Rodríguez-García Victoria, Saavedra-Orduz Zeidy, Torres-López Michael Alejandro, Moreno-Sarmiento Nubia

Article in Press

Phenotypic Detection of Extended Spectrum Beta-lactamase and Metallo Beta-lactamase production by Gram negative Uropathogens after exposure to gamma radiation

Eighty five uropathogen isolates were collected and differentiated on the basis of their Gram stain reaction. Among the collected isolates, 25 (29.4%) were Gram positive cocci and 60 (70.6 %) were Gram negative bacilli. Antibiotic susceptibility profile towards 15 different antibiotics concluded that impenime (IMP), amikacin (AK) and Pipperacillin/tazobactam (TZP) were the most effective against Gram negative. While,...

Author(s):Yehia Ahmed El-Zawahry, Lotfy Abd-El-Rehem Mousa, Eman Araby Salma Abd El monsef-Elnahass

Article in Press

Molecular characterization, biofilm production and sensitivity to the natural extract of Staphylococcus spp. from subclinical mastitis in goats

Mastitis is one of the most important diseases in dairy milk systems. Molecular studies of the principal agents that are involved, as well as tests of bacterial sensitivity to plant extracts, are currently the most common forms of research related to this disease. The aim of the present study was molecularly to characterize isolates from cases of subclinical mastitis and to investigate the production of biofilm associated...

Author(s):Rodolfo de Moraes Peixoto, Renata de Moraes Peixoto Araújo, Werônica de Souza Rocha, Luciana Jatobá e Silva Peixoto, Jackson Roberto Guedes da Silva Almeida, Gisele Veneroni Gouveia, Helinando Pequeno Oliveira, Mateus Matiuzzi da Costa

Article in Press

Distribution of β-Lactam Resistant Gram-Negative Bacteria Isolated from Clinical and Environmental Sources in Two Tertiary Hospitals in Makurdi, Nigeria

For appropriate control of infections, it is necessary to possess updated awareness about occurrence of the caus¬ative agents. Gram-negative bacteria are consider as an important microorganisms that cause hospital infections. Clinical multidrug resistant Gram-negative bacteria were obtained from clinical samples including Urine, High Vaginal Swab (HVS), Wound Swab (WS), Stools, Ear Swab (ES), Endocervical Swab (ECS),...

Author(s):Florence Bose Omoregbe and Obasola Ezekiel Fagade

Article in Press

Diversity of arbuscular mycorrhizal fungi (AMF) associated with cotton (Gossypium hirsutum L.) growing in the Far-North region of Cameroon

The present study was carried out with the aim of highlighting the diversity of arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of cotton grown in the Far-North region of Cameroon. To achieve this, composite soil and root samples were taken in six fields, chosen according to edapho-climatic conditions and types of cultural practices. After entrapping the glomales in the greenhouse, the roots of the trap...

Author(s):Ismael Haman Ramza, Honore Issa Koulagna, Abaka Abba Said, Philippe Kosma, Clautilde Megueni

Article in Press

Microbiological quality and safety of ready-to-eat foods from restaurants and food establishments in Yirgalem town, Southern Ethiopia

Foodborne illness is considered as one of the most important public health problems, especially in developing countries like Ethiopia. This study aimed to assess the microbiological quality and safety of ready-to-eat foods in Yirgalem town, Southern Ethiopia, from November 2016 to August 2017. A total of 160 food samples, including 40 'Injera firfir', 40 'Bayeaynet', 40 Vegetables and 40 spaghetti were collected and...

Author(s):Tsegaye Shamebo and Assefa Hamito

Article in Press

Impact of the use of treated wastewater in agriculture on the bacteriological quality of raw cow's milk on a farm in northeastern Algeria

This study aims to assess the impact of the use of treated wastewater (without chlorination) in farming and dairy cattle breeding. Milk samples were collected from a farm in northeastern Algeria. The treated wastewater from the treatment plant is used on this farm for different activities. The results obtained show that the average contamination of milks with total flora is 3.7.105 CFU /ml. fecal coliforms are present at...

Author(s):Chorfi Keltoum, Bendjemana Katia, Ammar Ayachi, Mahdi Fatima and Bouzidi Nour El Aimane

Article in Press

Integrated Management of Common Bacterial Blight (Xanthomonas campestris pv. phaseoli (Smith) Dye.) of Common Bean (Phaseolus vulgaris L.) in Southern Ethiopia

Common bacterial blight (CBB) caused by the bacterium Xanthomonas campestris pv. phaseoli is a major disease of common bean affecting its production in southern Ethiopia. This study was conducted to determine the effect of variety, seed treatment and foliar spraying frequencies of bactericide on disease severity, yield and yield components of common bean. Field research was conducted at Hawassa agricultural research center...

Author(s):Hailu Tontosha and Negash Hailu

Article in Press

Microbial Quality Assessment of Fresh and Semi-Cooked Nile tilapia Fillets and Hygienic Practice of Fish Handlers

Fish is a perishable food item and thus, its quality deteriorates rapidly during handling and proceeding. This could affect the health of consumers. Therefore, the aim of this study was to assess the microbial profile of fresh and semi-cooked fish fillet sold at Lake Hawassa landing sites and their surrounding hotels and restaurant, and asses food safety practices of fish handler’s in in the respective sites. For this, a...

Author(s):Fasika Admasu, Abraham Mikru and Kassaye Balkew Workagegn

Article in Press

COMPARATIVE STUDY ON THE IMMUNE RESPONSE AND SAFETY OF TWO COMMERCIAL INFECTIOUS BURSAL DISEASE VACCINES USED BY POULTRY FARMS IN KIGALI CITY, RWANDA

A total of ninety broilers chicks were reared for a defined period of time until they reached to two months of age after completion of vaccination program required for broiler chickens. The chicks were divided into three groups (A, B and C) of 30 chicks each, and housed separately into chicken pens divided into three compartments, they were fed daily and given water ad-libitum. Two vaccines against IBD were obtained from...

Author(s):Dushimirimana Emmanuel

Article in Press

THE INCIDENCE OF PSEUDOMONADS BOTH IN CLINICALLY AND ENVIRONMENTALLY AND ITS ANTIMICROBIAL RESISTANCE TO THE RANGE OF ANTIBIOTICS

This study was undertaken to explore the antimicrobial resistance of the organism Pseudomonas to the diverse classes of antimicrobials formulated in clinical practice and the prevalence of metallo-Beta-lactamases (mbls) produced by these isolates. Aims and objectives was to analyze the antibiotic resistance .The 97 strains were isolated both clinically and environmentally and were characterized by 16S molecular...

Author(s):DAS Ruchira, SINHA Papiya and PANDEY Piyush

Article in Press

Cytological changes in Solanum lycopersicum seedlings in response to some rhiozpheric fungi

Some rhizospheric fungi of Solanum lycopersicum were isolated from Cairo governorate and identified in Taxonomy Department, Ain-Shams University, Cairo city, Egypt. These isolated fungi were cultivated on soil extract medium enriched with 2% (w/v) glucose and 5% (w/v) yeast extract. The cytological effects of the metabolites of these fungi on the seeds of S. lycopersicum were examined after three different intervals (6,...

Author(s):Ahmed Fouad Afifi, Ehab Abdel-Razik Kamel, Amal Abdelsamei Elsaed, Mohamed Abdelaziz Foaad, Eman Mohamed Fawzy, Heba Ibrahim Abo-Elmagd

Article in Press

Assessment of Post-Harvest Fungal Diseases of Mango, Grape and Banana Saudi Arabia Market

A study was conducted to assess the extent of loss in mango, grape and banana fruits caused by fungal diseases during post-harvest. The loss due to fungal diseases was assessed at weekly intervals for four months (June to September 2018). A bout 300 samples of infected fruits were collected during this study, from different fruits market of Riyadh regions in Saudi Arabia. The results showed that, mango, grape and banana...

Author(s):Ahmed R. Al-Najada

Article in Press

Antibacterial effect of Anbarnesara on Lactobacillus casei, Pseudomonas aeruginosa and Staphylococcus aureus

Objective: In Iranian traditional medicine, Anbarnesara (derived from female donkey's stool) has a long history of therapeutic effect on oral ulcers and inflammatory conditions. The current study aims to investigate Anbarnesara extract (ANNAS) as a mouthwash on some common human pathogenic bacteria. Study design: The minimum inhibitory concentrations (MICs) of ANNAS 0.2% and chlorhexidine (CHX) 0.2% as the control...

Author(s):GOLNAZ NAHVI, Hassan Ali Shafiee, Gita Eslami, Hossein Pourabbasivand

Article in Press

Physicochemical Properties and Microbial Quality of Raw Cow Milk Collected from Debre Berhan Milk shed, Central Highlands of Ethiopia

The study was conducted in Debre Berhan Milk shed Areas, central highlands of Ethiopia, with the objectives to evaluate the physio-chemical characteristics, microbial load and hygienic practices of raw cow milk produced and marketed in study areas. A total of 359 milk samples were collected and analyzed physio-chemical and microbial examination. The study revealed that the proportions of respondents who practice washed...

Author(s):Admasu Lakew, Gebeyehu Goshu, Gezahegne Mamo, Ashenafi Mengistu and Tilaye Demissie

Article in Press

Resistance of Candida Albicans to antifungal drugs in Abidjan (Cote d’Ivoire)

This study aims to evaluate the resistance levels of strains of Candida albicans to the antifungals commonly used in Abidjan, Cote d’Ivoire. This is a prospective study that was carried out from July to October 2017 at the mycology laboratory of the Institut Pasteur of Cote d’Ivoire. 105 Candida isolates, obtained from swabs taken from patients receiving out-patient treatment, were seeded on chromogenic medium....

Author(s):C.G. Kouadio-Yapo, N.A.D. Aka, A.V. Bonouman-Ira, G.S.P Dou, K.H.G. Sonan, K.D. Zika, K.D. Adoubryn, M. Dosso

Article in Press

Biochemical and molecular characterization of yeasts isolated from Nigerian traditional fermented food products

Yeasts are very important in food production by affecting the quality and safety of different food products. Yeasts are commonly used in the production of beer, wine and bread. But many yeast species have been isolated from different African fermented foods. The present study was aimed at determine the biodiversity of yeasts isolated from selected Nigerian fermented foods. A combination of phenotypic and molecular tests...

Author(s):Adesokan Isaac Ayanniran, Sanni Abiodun I and Marc-Andre Lachance

Article in Press

Quality assessment of bacterial load present in drinking water in Woreta town, Ethiopia.

Access of adequate and safe water is a universal human need. Lack of safe and adequate water supply causes health risk and the situation is serious in developing countries The aim of this study was to determine the bacterial load, physicochemical quality of drinking water in wells, reservoir, taps and household storage containers water samples and to investigate the hygiene and sanitation practices of the consumers in...

Author(s):Kindu Alem

Article in Press

Stability and Antioxidant Activity of Natural Red pigment by Pseudomonas stutzeri ZH-1

Natural pigments are widely used in textiles, leather, food and other materials, due to they are non-toxic and easy to extract. In the present study, stability and antioxidant activity of a natural red pigment was investigated from Pseudomonas stutzeri ZH-1 which was first isolated and identified from the sludge of Fenhe River, Linfen, Shanxi Province. The results of stability tests showed the red pigment had a relative...

Author(s):Si-yu Wang and Qing-ping Hu

Article in Press

Bacteriological study of goats and sheep livers slaughtered in slaughterhouse under Federal Inspection Service in Bahia

The study aimed to verify the occurrence of aerobic bacteria causing liver macroscopic lesions in sheep and goats, besides describing the susceptibility profile to antimicrobials and the pathogenic potential of the isolated bacterial species. The samples were collected during the slaughter of 1,270 animals in slaughterhouse under Federal Inspection Service (SIF) in the municipality of Feira de Santana, Bahia, Brazil....

Author(s):Lorena Santos Brandão, Maurício Costa Alves da Silva, Tatiana Pacheco Rodrigues, Rebeca Ayala Rosa da Silva

Article in Press

Isolation, Biochemical and Molecular Characterizations of Riboflavin Producing Lactic acid Bacteria from Nigerian Fermented Food Products

The purpose of this research was to explore lactic acid bacteria (LAB) from Nigerian fermented foods that can be used to produce riboflavin. In this study, LAB were isolated from “wara”, “nono”, and “ogi”. Total number of colonies of LAB in triplicates ranged from 3.9 x 106 CFU ml 1 to 4.2 x 106 CFU ml 1. A total of 321 lactic acid bacteria were isolated. Of these LAB isolates, 129 (40%) belong to the genus Lactobacillus,...

Author(s):Joy Abiola Onipede and Oluwatoyin Rebecca Afolabi

Article in Press

Combination of genetic tools to discern Bacillus species isolated from hot springs in South Africa

Using phylogenetic analysis of the 16S rRNA gene 43 gram-positive, spore-forming bacteria of the phylum Firmicutes were isolated, cultured and identified from five hot water springs in South Africa. Thirty-nine isolates belonged to the family Bacillaceae, genus Bacillus (n = 31) and genus Anoxybacillus (n = 8), while four isolates belonged to the family Paenibacillaceae, genus Brevibacillus. The majority of isolates fell...

Author(s):Jocelyn Leonie Jardine and Eunice Ubomba-Jaswa

Article in Press

Microbiological Quality of Leafy Vegetables Irrigated with Wastewater in Harar Town Vegetable Farm, Eastern Ethiopia

Food safety issues are of growing concern to consumers globally because of the risks associated with consumption of foods contaminated with pathogenic microbes. Harar town vegetable farm is known to produce vegetables irrigated with wastewater. To what extent these vegetables are contaminated with pathogenic microbes was not known. The study was aimed to examine the extent of microbial contamination of vegetables. Thus, a...

Author(s):Getachew Alamnie, Ameha Kebede, Sissay Menkir

Article in Press

Antibiotic Susceptibilities, MIC50 and MIC90 of Clinical Isolates of Methicillin Resistant Staphylococcus aureus (MRSA)

Methicillin-Resistant Staphylococcus aureus is evolved strains of S. aureus resistant to all β-Lactam antibiotics and is among important nosocomial pathogens worldwide. The study aimed to determine the antibiotic susceptibilities of clinical MRSA strains to first-line drugs and drugs of last resort in the treatment of MRSA infections. Clinical samples were collected, screened for MRSA and minimum inhibitory concentrations...

Author(s):Uzal Umar, Auwalu Uba, Olubukola M. Oyawoye

Article in Press

Isolation and characterization of Edwardsiella tarda from wild fish from the gulf of Lake Tana, North Western Ethiopia

Edwardsiellosis is one of the important bacterial diseases of fish causing severe economic losses in aquaculture worldwide. In Ethiopia, information on fish pathogenicEdwardsiella species is scanty. This study was conducted to isolate and determine the extent of occurrence of fish pathogenic Edwardsiella species from apparently healthy fish harvested for human consumption from the gulf of Lake Tana, Ethiopia....

Author(s):Yoseph Kerie, Anuar Nuru, Liyuwok Tesfaw, Takele Abayneh

Article in Press

A Fluorescence based assay for detection of Listeria monocytogenes using wheat germ agglutinin

Purpose: This study was designed with the objective to check the ability of Wheat Germ Agglutinin (WGA) lectin to specifically bind with Listeria monocytogenes based on fluorescence based assay. Methods: The fluorescence activity of WGA, mixture of WGA and L. monocytogenes and WGA with different sugars was evaluated using Tecan multimode plate reader by an optimized protocol. Association constant (Ka) of WGA with...

Author(s):Raghu H. V , Naresh Kumar, Anjali M. K, Harsimran Kaur and Brijesh Kumar

Article in Press

Bacteriological Assessment of Hospital Air and Selected Surfaces in Three Referral Hospitals in Makurdi Middle Belt Nigeria

Hospital environment needs more attention because of the influx of people into the environment for medical checkup and other services. This study unveils the incidence of bacteria isolated from air and selected surfaces in three referral hospitals (City hospital, Federal Medical Centre, Wadata and Saint Theresa Hospital) in Makurdi Middle Belt Nigeria. Fifty (50) bacteria were isolated; hospital air (26%), bed rails (16%),...

Author(s):Emmanuel Olumuyiwa Onifade, Innocent Okonkwo Ogbonna, Joseph Ikwebe, Tosin Adebola Ode, Stephen Olaide Aremu, Babatunde Fatoke, Oluwole Opeyemi Owoyemi, Ebenezer O. Abeleje

Article in Press

Root growth in tomato seedlings in response to bacterial inoculation Serratia sp.

Growth-promoting rhizobacteria are soil bacteria that inhabit the surrounding root, and are directly or indirectly involved in promoting plant growth and development. The objective of this study was to verify if the mechanism of growth promotion of the bacterium is similar to that promoted by Polyethylene glycol (PEG) and to compare the possible effects of water stress on the tomato against the effects of inoculation of...

Author(s):Ana Carla Pinheiro Lima; Eduardo de Assis Lima, Débora Alves Gonzaga da Silva Ballesteiro Pereira, Leonardo Oliveira Médici

Article in Press

Assessment of Microbiological Quality and Drug Resistance Patterns of Raw Vegetables Irrigated with Hasassa River, West Arsi Zone, Oromia Region, Ethiopia

Irrigation scheme is among major transmission modes of enteric human pathogens. Irrigation of vegetables with polluted water and untreated wastewater is a common practice worldwide. This practice is common in urban areas of low-income countries including Ethiopia, where there is increasing demands for fresh vegetables with poor wastewater treatment. The aim of the present study was to assess the microbial quality and...

Author(s):Edao Gelgelu, Zerihun Belay, Bayissa Chala

Article in Press

Isolation and characterization of Bacillus species for the production of lipopeptides and its in vitro management against root knot nematode, Meloidogyne incognita

Bacillus species are found to be abundant in the terrestrial ecosystem. In this study, native Bacillus species associated with different horticultural crops growing in different districts of Tamil Nadu were isolated and identified. In total of 40 isolates collected, 30 isolates were identified as Bacillus species using morphological and biochemical characterization. These 30 Bacillus isolates were investigated for their...

Author(s):SANKARI MEENA

Article in Press

Antibacterial Activity of Marine Yeasts by Resazurin Assay

Resazurin is an oxidation–reduction indicator used for the evaluation of cell growth, particularly in various cytotoxicity assays. It has been used over five decades to monitor bacterial and yeast contamination of milk, and also for assessing semen quality. This chemical reduction is mainly used in viability assays for mammalian cells. In the present study, antibacterial activity of marine yeasts is assessed by using...

Author(s):Senthil raja P

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