African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12421

Table of Content: 20 March 2012; 11(23)

March 2012

Molecular detection of TasA gene in endophytic Bacillus species and characterization of the gene in Bacillus amyloliquefaciens

  TasA, the gene which play an important role in bacteria development, physiology and bacteria biofilm formation in Bacillus species was detected in the endophytic bacteria by polymerase chain reaction (PCR) amplification. In ten endophytic Bacillus strains tested, TasA gene was readily detected in Bacillus amyloliquefaciens PEBA20 and 7...

Author(s):   Liang Xu, Yang Cao, Xiang-Tian Yin, De-Fa Deng, Li-Na Xu and Zhen-Yu Liu        

March 2012

Semi-quantitative analysis of endometrial receptivity marker mRNA expression in the mid-secretory endometrium of patients with uterine fibromas

  In fertile women, expression of molecular marker of endometrial receptivity, HOXA11, leukemia inhibitory factor (LIF) and basic transcriptional element binding protein 1 (BTEB1), rises during the luteal phase with the peak occurring during the implantation window. We evaluated the transcript levels of HOXA-11, LIF and BTEB1 in the mid-secretory endometrium of infertile patients with uterine fibroid...

Author(s):   Naser Shokrzadeh and Zohreh Alizadeh,      

March 2012

Analysis of the copy number of exogenous genes in transgenic cotton using real-time quantitative PCR and the 2-△△CT method

Presence of multiple copies of a transgene has been found to associate with gene silencing that may manifest early or only over a period of time. As such, transgenic copy number should be analyzed as soon as possible. The commonly used method to analyze gene copy number, Southern blotting, has been found to be unreliable for determination of gene copy number when rearrangement or tandem repeats integration occurred. In...

Author(s):   Xiaojie Yang, Fuguang Li, Chuanliang Liu, Xueyan Zhang, Kun Liu, Weiping Fang, Zhixia Wu, Deyi Xie, Chaojun Zhang, Qianhua Wang and Fu′an Zhao        

March 2012

Study on soluble expression of glutamate dehydrogenase from tea plant in Escherichia coli using fusion tags

  Glutamate dehydrogenase (GDH; EC1.4.1.2) catalyses the reversible amination of 2-oxoglutarate for the synthesis of glutamate using ammonium as a substrate. Herein, ahomology-based reverse transcription polymerase chain reaction (RT-PCR) strategy was employed to search for cDNAs encoding GDH from Camellia sinensis cv Longjing 43, an excellent variety of tea tree most suitable...

Author(s):   Peng Wang#, Ping Song#, Xue Li, Rui-Rui Su, Hui Wang and Guo-Ping Zhu    

March 2012

Improvement of daptomycin yield by overexpression of the accessory genes of daptomycin gene cluster

  The effects of the accessory genes flanking the non-ribosomal peptide synthetase (NRPS) genes on daptomycin production were investigated by overexpression under the control of ermE* promoter via the integrative Escherichia coli–Streptomycesvector pIB139. The yield of daptomycin was promoted significantly when either of the upstream accessory genes of dptE and dptF or...

Author(s): Guanghai Yu, Yuansen Hu, Liang Huang and Jianhang Qu      

March 2012

Examining kinetic parameters of fermentation process in batch cultures on the production of alkaline protease

  Alkaline proteases are important group of enzymes because of their use in many industrial applications such as in detergent materials for the removal of the protein stains on clothes. The aim of this study is to investigate the kinetic parameters of the fermentation process inspected for alkaline protease production by using a localBacillus clausii. In this way, it is carried out under fed-batch operations of...

Author(s):   M.T. Hamed Mosavian , J. Sargolzaei ,  F. Tabandeh, H. R. Hosseinian Moghaddam and B. Yakhchali        

March 2012

Herbicidal activity of Pennisetum purpureum (Napier grass)

  This study investigates potential herbicidal effects of Pennisetum purpureum extracts on two selected weed bioassay species. Ethyl acetate extract of P. purpureum was able to suppress germination of bioassay species by 41%. Although, it had no phytotoxic activity on the root or shoot growth of bioassay species, it had caused leaf discs of bioassay species to turn dark brown....

Author(s):   M. Z. Norhafizah, B. S. Ismail and T. S. Chuah      

March 2012

Incidence and distribution of seed-borne fungi associated with wheat in Markazi Province, Iran

  53 seed samples collected from harvested seed loads of irrigated wheat fields in Markazi province in the central of Iran was used for this study. Isolation and identification of seed-borne fungi were conducted according to standard tests described by the International Seed Testing Association (ISTA). A total of 15 fungal species including Tilletia laevis, Tilletia tritici, Ustilago...

Author(s):   Masomeh Hajihasani, Abolfazl Hajihassani and Shahab Khaghani        

March 2012

Molecular cloning, characterization and expression analysis of heat shock protein 90 (HSP90) from the mud crab Scylla paramamosain

  Heat shock protein 90 (HSP90) is a highly conserved protein and plays an important role in maintaining the structure of protein, participating in the immunity and regulating the cell cycle. Using the rapid amplification of cDNA ends (RACE) techniques, the cDNA sequence of HSP90 gene (designated Sp-HSP90) was cloned and characterized from the mud crab Scylla paramamosain. The full-length cDNA...

Author(s):   Fenying Zhang, Keji Jiang, Dan Zhang, Chunyan Ma, Hongyu Ma and Lingbo Ma        

March 2012

Osmopriming improves tomato seed vigor under aging and salinity stress

  This study investigates the effects of osmopriming on tomato (Lycopersicon esculentum M.) hybrid seed vigor under aging and salinity stress. Tomato seeds of the ZZ1 hybrid variety, stored for four years under natural (aged) or -20°C (unaged) conditions were primed in 10% (w/v) polyethylene glycol (PEG) solution for 2 d at 20±1°C in the dark. The seed vigor was evaluated at...

Author(s):   Mengyu Zhang#, Zhoufei Wang#, Lingling Yuan, Congfei Yin, Jinping Cheng, Ling Wang, Ji Huang and Hongsheng Zhang      

March 2012

Effect of crude canine pituitary extract (cCPE) on the in vitro production of progesterone and nuclear maturation of canine oocytes

  We examined the effect of crude canine pituitary extract (cCPE) on the in vitronuclear maturation of canine oocytes and production of progesterone by cumulus cells. cCPE was extracted from canine pituitaries and the concentrations of canine follicle stimulating hormone (FSH) and luteinizing hormone (LH) were determined. Cumulus oocyte complexes (COCs) were harvested from anestrus cycle ovaries and...

Author(s):   Jin-Dan Kang, Hyo-Sang Lee, Shuang Liang, Suo Li, Yue Lu, Il-Keun Kongand Xi-Jun Yin        

March 2012

Optimal conditions for mycelia biomass and extracellular polysaccharides of Grifola frondosa: Effect of agitation speed, inoculum ratio and initial pH

  Grifola frondosa, a medicinal and edible mushroom, has shown different bioactive compounds, such as polysaccharides with anticancer and other therapeutic properties. In the present research, the mycelia of G. frondosa were cultivated in shaking flasks to investigate the effects of agitation speeds, inoculum ratios, and initial pH on pellet morphology, biomass, and extracellular polysaccharide...

Author(s):   Huu-Nghi Nguyen, Tzu-Ching Wang, Ta-Chen Lin and Jia-Hsin Guo      

March 2012

Purification and characterization of an extracellular esterase from a moderately halophilic bacterium, Halobacillus sp. strain LY5

  A newly moderate halophilic strain LY5 producing extracellular esterase was isolated and identified. Biochemical and physiological characterization, along with 16S rRNA gene sequence analysis placed the isolate in the genus Halobacillus. Esterase production started from the mid-exponential phase of bacterial growth and reached a maximum level during the mid-stationary phase. The enzyme was highly active...

Author(s):   Xin Li, Hui-Ying Yu and Yi-Feng Lin        

March 2012

Examining kinetic parameters of fermentation process in batch cultures on the production of alkaline protease

  Alkaline proteases are important group of enzymes because of their use in many industrial applications such as in detergent materials for the removal of the protein stains on clothes. The aim of this study is to investigate the kinetic parameters of the fermentation process inspected for alkaline protease production by using a localBacillus clausii. In this way, it is carried out under fed-batch operations of...

Author(s):   M.T. Hamed Mosavian , J. Sargolzaei ,  F. Tabandeh, H. R. Hosseinian Moghaddam and B. Yakhchali          

March 2012

In vitro anti-hyperglycaemic effect of glucocapparin isolated from the seeds of Boscia senegalensis (Pers.) Lam. ex Poiret

  Glucocapparin (I) used in this study was isolated from the seeds of Bosciasenegalensis (family Capparidaceae). The structure of (I) was determined on the basis of an extensive analysis of the spectroscopic data. Brine shrimp lethality bioassay of (I) showed a marked significant cytotoxic activity with LC50 = 16.482 μg/ml. Compound (I) reduced the liberation of glucose from the...

Author(s):   Mahamat Nour Adam Sakine, Yaya MAHMOUT, M. G. Dijoux-Franca, Joachim Gbenou and Mansourou Moudachirou      

March 2012

Antioxidant properties of polysaccharides from Laetiporus sulphureus in submerged cultures

  Intracellular polysaccharides (IPS) and exopolysaccharides (EPS) are isolated respectively from mycelia and filtrates of submerged culture by Laetiporus sulphureusin a 20-L stirred tank bioreactor with corresponding antioxidant properties investigated. Effective productions of IPS and EPS by submerged cultures of L. sulphureus are available. Number of average molecular weights (Mn) and...

Author(s):   Ming-Yeou Lung and Wei-Zhong Huang        

March 2012

Oral pathology in the Iberian Neanderthals

  In 1994, a series of human bones was found at the Sidrón cave in Borines (Concejo de Piloña, Asturias), Spain. Since the investigators suspected that they were dealing with human remains from the Spanish Civil War (1936-1939), the bones were collected by the Civil Guard, but were not subjected to archeological scrutiny. The finding was reported then to the corresponding authorities, who had them...

Author(s):   Lopez-Valverde A., López-Cristiá M., Prados-Frutos J. C., Gómez de Diego R., de Vicente J. and Cutando A.      

March 2012

Volatile composition of the peel and leaf essential oils of Citrus nobilis Lour. var deliciosa Swingle

  The fruits and leaves of Citrus nobilis Lour. var deliciosa Swingle were collected from south of Iran and their essential oils were analysed by gas chromatography-mass spectrophotometry (GC-MS). The oil yields of the fresh peel and leaves obtained separately by hydrodistillation were 1.2 and 0.2% (V/W), respectively. 17 components accounting for 99.2% of the peel oil and 34 components...

Author(s):   Jinous Asgarpanah, Saeed Mohammadi Motamed and Simin Tomraee        

March 2012

New approach for regional crop yield gap analysis in the Borujen Plain, Iran

  This study was performed to analyze the regional crop yield gap for potential and water-limited production situations in the cold-semiarid climate at Borujen basin, Iran. Experimental data were used for model calibration and evaluation of WOFOST as a crop growth simulation model. WOFOST divides wheat growth into three key development stages: 1) emergence, 2) flowering and 3) physiological maturity. The length...

Author(s):   A. Ghanbari and J. Taei-Semiromi        

March 2012

The impact of carbon dioxide in stored-product insect treatment with phosphine

  In laboratory experiments, toxicity of carbon dioxide and carbon dioxide - phosphine mixture was investigated against 4 species of stored-product insects. In empty-space trials, estimates of the median lethal doses of carbon dioxide against adults ofOryzaephilus surinamensis (L.), Lasioderma serricorne (F.) and eggs of Plodia interpunctella (Hǘbner) and Cadra...

Author(s):   Orouj Valizadegan, Ali Asghr Pourmirza and Mohammad Hassan Safaralizadeh        

March 2012

The effects of reciprocal cross on inheritance of DNA methylation in cotton (Gossypium hirsutum)

  DNA methylation plays an important role for regulation of gene expression. To study the inheritance of DNA methylation, we selected two F1 plant population by reciprocal cross with two cotton lines Zongcaixuan No.1 and HY428, and analyzed the variations of DNA methylation levels and patterns in F1 generations by methylation sensitive amplified fragment length polymorphism (MSAP)...

Author(s):   Jun Wei#, Honghong Fan#, Tingchun Li, Wanghua Wang, Ning Guo, Zhengpeng Li, Yongping Cai and Yi Lin        

March 2012

Variation in seedling morphology of Turkish fir (Abies nordmanniana subsp. bornmulleriana Mattf)

  In this study, the genetic variation of some seedling characteristics of Turkish fir was investigated. A total of 303 trees were selected from 17 plots and 10 seedlings from each tree were used. Fifteen morphological characteristics were determined, including root collar diameter, seedling height, total needle, bud length and width, epycotyl length, needle length and width of 1 year seedlings and root collar...

Author(s):   Hakan SEVIK    

March 2012

Isolates of Mycoplasma mycoides subspecies mycoides (SC) in small ruminants in Sahel zone of Nigeria and its implications on disease control

  A study on the isolation of Mycoplasma mycoides subspecies mycoides (SC) in small ruminants and its implication on disease control was carried out in the Sahel zone of Nigeria. This was achieved by the examination of pneumonic lesions in apparently normal and affected lungs of sheep and goats slaughtered at Maiduguri municipal abattoir. A total of 400 lungs (200 each from sheep and goats)...

Author(s):   Egwu G. O., Adamu M., Mshelia G. D. and Bukar-Kolo Y. M.      

March 2012

Cloning and expression of a tomato glutathione S- transferase (GST) in Escherichia coli

  Glutathione S- transferases (GSTs) fulfill a diverse range of functions in an organism. In a previous study, a putative glutathione S-transferase gene (ShGSTU1) from a wild-type tomato, Solanum habrochaites G1.1560, was identified to be a key gene in pathogen resistant response against powdery mildew in tomato. In this study,ShGSTU1 was cloned into plasmid pET-28a, efficiently expressed...

Author(s):   Dongli Pei, Jinping Ding, Zilei Duan, Meng Li, Yiyang Feng and Chengwei Li,        

March 2012

Studies on the intiation of callusing and regeneration of plantlets in three different basal media with varied plant growth regulators for the micropropagation of Anthurium scherzeriaum using leaf and spathe as explants  

Anthurium scherzeriaum is one of the most common ornamentals used in floriculture industry. However, there is shortage of planting material supplied to the cultivators. To overcome this problem, many breeders adopted micropropagation techniques. The usage of high dosage of plant growth regulators in the media resulted in mutation and loss of potentiality in the regeneration. An attempt was made to derive a suitable...

Author(s): Jagan Mohan Reddy and A. K. Bopaiah