Micropropagation of wild fennel (Foeniculum vulgare var. vulgare) via organogenesis and somatic embryogenesis
April 2015
Wild fennel (Foeniculum vulgare var. Vulgare) is a perennial aromatic herb. It is native to the Mediterranean region and currently it iscultivated as an annual or perennial herb worldwide. Dried ripe fruits are commonly used plants part for obtaining essential oil. Fruits oil of wild fennel contains many volatile oils, such as α-pinene, phellandrene, p-cymene, fenchone, estragole, anethole and anisaldehyde....
A study on the formation and development of Panax bipinnatifidus Seem. adventitious root
July 2014
This study aimed to generate adventitious roots from root and stem-derived callus of Panax bipinnatifidus Seem. Callus formation was observed best (83.3%) in MS media supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1 mg/L indolebutyric acid (IBA). Callus generated adventitious roots in MS media supplemented with 2.0 mg/L IBA at high frequency (66.7%). The optimal culture condition for the...
Markers are shared between adipogenic and osteogenic differentiated mesenchymal stem cells
May 2013
The stem cell differentiation paradigm is based on the progression of cells through generations of daughter cells that eventually become restricted and committed to one lineage resulting in fully differentiated cells. Herein, we report on the differentiation of adult human mesenchymal stem cells (hMSCs) towards adipogenic and osteogenic lineages using established protocols. Lineage specific genes were evaluated by...
Morphology and histology of the alimentary tract of adult palm weevil, Rhynchophorus phoenicis Fabricius (Coleoptera: Curculionidae)
May 2013
Palm weevil (Rhynchophorus phoenicis) is a notorious pest of oil palm trees, worldwide. Investigations were conducted on the alimentary tract of the adult insect. Results of the morphology and histology of the alimentary tract revealed that the alimentary tract consisted of a foregut, which was made up of a buccal cavity, oesophagus, crop and proventriculus. The crop big or small and the proventriculus had bristle...
Future of dentistry, nanodentistry, ozone therapy and tissue engineering
April 2013
Future of dentistry has become one of the most unique resources and providers of this oral systemic approach. In determining the patient's unique needs, it is becoming increasingly important for the dentist to work with other cutting-edge, integrative practitioners to fully assess the individual requirements of the patient before, during, and after dental care. Witnessing the beginning of truly groundbreaking...
3-D osteoblast culture for biomaterials testing
April 2013
Micromass cell cultures, osteomicrospheres, were formed by aggregation of primary osteoblasts. Cell differentiation during sphere formation and the integrity of the osteomicrospheres was evaluated by analyzing the immunohistochemical expression of osteonectin, osteocalcin, and collagen type I. Transmission electron microscopy facilitated the proof of the tissue-like microstructure inside the osteomicrospheres and the...
Study of cephalometric parameters among 4 to 11 years old Persian girls resident in Iran
May 2012
Anthropometry is applied in medical profession such as maxillofacial surgery, growth and development studies, plastic surgery, bioengineering and non-medical branches such as like shoe-making and eye-glasses industries. The aim of the present study was to determine facial and cranial parameters among 4 to 11 years old Mashhadian young girls in order to assess growth pattern in this age range. 564 Persian girls aged...
Round-bottomed Honeycomb Microwells: Embryoid body shape correlates with stem cell fate
May 2012
The differentiation of embryonic stem cells (ESC) into tissue-specific cells utilizes either monolayer cultures or three-dimensional cell aggregates called embryoid bodies (EB). However, the generation of a large number of EB of controlled sizes can be challenging and labor intensive. Our laboratories have developed a simple, robust, ultra-rapid, and inexpensive design of Honeycomb Microwells for generation of EB. Here,...
An investigation on cephalometric parameters in Iranian population
April 2012
Anthropometry is the biological science of human body measurement. Anthropometry is applied in medical profession such as maxillofacial surgery, growth and development studies, plastic surgery, bioengineering and non-medical branches such as like shoe-making and eye-glasses industries. The aim of this study was to determine Iranian cephalometric parameters and cranial and facial anthropometric ratios. This cross...
Mimicry of a natural, living intra-epidermal micro pattern used in guided tissue regeneration of the human epidermis
April 2012
The methodology for the isolation of the living, intra-epidermal micro pattern using a tissue culture method is described. The disassembly of the stripped off epidermis (after Dispase digestion at 37°C) into epidermal brown rosettes (their morphology under a phase contrast microscope) constitute intra-epidermal micro patterns. The stepwise reasoning in the recognition of epidermal basal layer rosettes and the...
Achievement of balanced oncogenes and tumor-suppressor genes activity in normal and malignant cells in vitro and in vivo
November 2011
The main goal is connected with providing, on the one hand, of active tumor-suppressor genes for prevention of eventual malignant transformations, and, on the other hand, of functionally active oncogenes for prevention of early aging and death, both in vitro and in vivo. Modulation of an adequate immune control was also necessary, and in this way any eventual unwished side effects from the genetic...
Mesenchymal stem cells and acquisition of a bone phenotype: An ion channel overview
November 2011
Bone remodeling is a physiological process determined by the sequential and coordinated interaction of osteocytes, osteoclasts, osteoblasts and angiogenesis. During bone repair, osteoblastic cells, originated from mesenchymal stem cells (MSCs), are highly regulated to proliferate and to produce an osteogenic matrix, thus forming a new bone. MSCs are multipotential and undifferentiated cells that are present in the adult...
Micropropagation of Maerua oblongifolia: A rare ornamental from semi arid regions of Rajasthan, India
August 2011
A method for in vitro regeneration of Maerua oblongifolia (Capparaceae) from nodal shoot explants is outlined. Percent shoot response with multiplication rate (21.1± 2.33) shoots per explant (30 mm length) was achieved when cultured on semisolid Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with 2.0 mgl-1 of (Benzylaminopurine) BAP + additives (25.0 mgl-1adenine...
Ultrasonic analyses algorithm on an ex vivo produced oral mucosal equivalent
August 2011
This study examines the use of high-resolution ultrasound with an analyses algorithm to accurately monitor development of an ex vivo produced oral mucosal equivalent (EVPOME). We used ultrasonic profilometry to examine EVPOME development as seeded cells on its surface proliferate. As these engineered structures develop, seeded cells stratify from their differentiation and produce a keratinized protective upper...
Challenges of bioengineering and endodontics
July 2011
Stem cell research and scaffolding are now the buzz words in basic science pulp researches. The endodontic community needs to enhance its clinical understanding of the vital pulp and dentin, and embrace new treatment modalities. Several regenerative techniques have been described, while therapies involving stem cells, growth factors and scaffolds have been proposed. Each technique has its own advantages and...
Ultrastructural changes in the tight intercellular junctions of the endoneurial blood vessels following sural nerve crush injury in rats
July 2011
The objective of this study was to investigate the ultrastructural changes in the tight intercellular junctions of the endoneurial blood vessels after nerve injury. Twenty-one adult male Sprague-Dawley rats were used in the study (3 animals as a control group and left sural nerves of 18 animals were subjected to the crush). At intervals of 1, 2, 3, 4, 5 and 6 weeks after crush, 3 rats /sitting were killed and the fixed...
Fibrin sealant as scaffold can be a suitable substitute to autograft in short peripheral nerve defect in rats
June 2011
There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. In this study, the process of nerve regeneration through a fibrin scaffold was examined. 45 male Wistar rats were randomly divided into one control, Autograft (Auto) and two experimental groups, Epineurum (Epi) and Fibrin scaffold (Fib). Right sciatic nerve was exposed of which 5 mm was cut. The...
Healthcare workers uniforms (HCWU)
June 2011
There is a major concern for healthcare workers (HCW) regarding transmission of bacteria to and from their patients. Because of this potential contamination, protection is a major issue. Healthcare workers uniforms (HCWU) are often used as barriers to help eliminate or reduce the risk of infection for both the doctor and the patient. Without sufficient barriers, harmful bacteria can reach and penetrate the skin of...
Healthcare workers uniforms (HCWU)
June 2011
There is a major concern for healthcare workers (HCW) regarding transmission of bacteria to and from their patients. Because of this potential contamination, protection is a major issue. Healthcare workers uniforms (HCWU) are often used as barriers to help eliminate or reduce the risk of infection for both the doctor and the patient. Without sufficient barriers, harmful bacteria can reach and penetrate the skin of...
Fibrin sealant as scaffold can be a suitable substitute to autograft in short peripheral nerve defect in rats
June 2011
There is considerable evidence that peripheral nerves have the potential to regenerate in an appropriate microenvironment. In this study, the process of nerve regeneration through a fibrin scaffold was examined. 45 male Wistar rats were randomly divided into one control, Autograft (Auto) and two experimental groups, Epineurum (Epi) and Fibrin scaffold (Fib). Right sciatic nerve was exposed of which 5 mm was cut. The...
Influence of osteogenic supplements on the osteoclastogenesis of human monocytes
May 2011
A basic requirement for bone remodeling is the communication between bone forming osteoblasts and bone resorbing osteoclasts – the so-called cross talk. Corresponding in vitro co-culture models might be a valuable technique in order to investigate the influence of novel biomaterials on the cross talk. Assuming that both cell types are derived by precursor cells, the role of the common osteogenic...
In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var.LGG-29)
May 2011
An efficient and direct shoot bud differentiation and multiple shoot induction from embryo explants of pigeon pea (Cajanus cajan L.) has been achieved. The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of cytokinin. Explant embryo were cultured on Murashine and Skoog (MS) medium augmented with different concentrations of Benzyl amino purine (BAP). Among the...
In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var.LGG-29)
May 2011
An efficient and direct shoot bud differentiation and multiple shoot induction from embryo explants of pigeon pea (Cajanus cajan L.) has been achieved. The frequency of shoot bud regeneration was influenced by the type of explant, genotype and concentrations of cytokinin. Explant embryo were cultured on Murashine and Skoog (MS) medium augmented with different concentrations of Benzyl amino purine (BAP). Among the...
Influence of osteogenic supplements on the osteoclastogenesis of human monocytes
May 2011
A basic requirement for bone remodeling is the communication between bone forming osteoblasts and bone resorbing osteoclasts – the so-called cross talk. Corresponding in vitro co-culture models might be a valuable technique in order to investigate the influence of novel biomaterials on the cross talk. Assuming that both cell types are derived by precursor cells, the role of the common osteogenic supplements...
Recent studies on the pullout strength behavior of spinal fixation
April 2011
Spinal fixations with pedicle screws are widely used nowadays in order to provide spine stability and correcting spinal deformity. The pullout strength of pedicle screws can be evaluated by means of pullout strength testing. This paper reviews recent experimental and finite element analysis evidence concerning the factors that affecting the pullout strength of pedicle screws in various bone materials. Cadaveric bones...
Use of 3-dimensional environments to engineer osseous-like tissue
April 2011
We have previously reported enhanced aggregate formation and accelerated mineralization of human mesenchymal preosteoblastic cells (HEPM) when cultured without scaffolds in 3D-rotary wall vessel chambers. From those studies we hypothesized that, rotary wall vessel environments could be used to engineer osseous tissue. Thus, HEPM preosteoblastic cells were cultured in rotary wall vessels for up to 7 days. Progression of...
Recent studies on the pullout strength behavior of spinal fixation
April 2011
Use of 3-dimensional environments to engineer osseous-like tissue
April 2011
We have previously reported enhanced aggregate formation and accelerated mineralization of human mesenchymal preosteoblastic cells (HEPM) when cultured without scaffolds in 3D-rotary wall vessel chambers. From those studies we hypothesized that, rotary wall vessel environments could be used to engineer osseous tissue. Thus, HEPM preosteoblastic cells were cultured in rotary wall vessels for up to 7 days. Progression of...
Time trends, and survival of patients with oral and pharyngeal malignancies
March 2011
An accurate assessment of oral and pharyngeal malignancies in cancer treatment trends, and survival of the disease was missing in Egypt. Accordingly, all new cases treated in Alexandria and El Behira governorates during the last decade were studied retrospectively. Data were collected through all accessible archives using a special data collection sheet. The total populations of different governorates were obtained from...
Nucleofection an efficient non-viral transfection technique forIFN-t-EGFP gene expression study in Sahiwal cattle fibroblast cells
March 2011
Viral based techniques were considered to be the most efficient systems to deliver DNA into fibroblast cells as they show high transgene expression in many cellular models. Viral approaches are complicated by immune response, intracellular trafficking potential mutations and genetic alterations due to integration. The nucleofector TM technology is electroporation based gene transfer technique which has been proved to be...
Time trends, and survival of patients with oral and pharyngeal malignancies
March 2011
An accurate assessment of oral and pharyngeal malignancies in cancer treatment trends, and survival of the disease was missing in Egypt. Accordingly, all new cases treated in Alexandria and El Behira governorates during the last decade were studied retrospectively. Data were collected through all accessible archives using a special data collection sheet. The total populations of different governorates were obtained from...
Nucleofection an efficient non-viral transfection technique forIFN-t-EGFP gene expression study in Sahiwal cattle fibroblast cells
March 2011
Viral based techniques were considered to be the most efficient systems to deliver DNA into fibroblast cells as they show high transgene expression in many cellular models. Viral approaches are complicated by immune response, intracellular trafficking potential mutations and genetic alterations due to integration. The nucleofector TM technology is electroporation based gene transfer technique which has been proved to be...
Optimization of medium conditions for efficient plant regeneration from embryo of cotton (var.narishima)
February 2011
An efficient and rapid regeneration protocol was developed using embryo from germinating seedlings of cultivar of cotton (var.narishima) as an explant. A vertical slit given in the apex of embryo shoot regeneration was successful on murashige and skoog medium with 2 mg/L benzyl amino purine (BAP). The shoots were sub cultured in every 15 days in regeneration medium and later they were transferred to rooting medium (1...
Synthesis, spectroscopy and biological studies of Nickel (II) complexes with tetradentate Schiff bases having N2O2 donor group
February 2011
Complexes of nickel(II) of N,N’-disalicylidene-3,4-diaminotoluene(H2L1), N,N’-bis(3,5-di-tert-butylsalicylidene)-1,3-diaminopropane(H2L2),tetrathiafulvalene-N,N’-phenylene bis (salicylideneimine)(H2L3), o-hydroxybenzaldehyde, o-hydroxyacetophenone ethylene diamine (H2L4) and 1-phenylbutane-1,3-dionemono-S-methylisothio- semicarbazone with 5-phenylazo- o-hydroxybenzaldehyde (H2L5) have been synthesized...
Exogenous NGF favors initiation of lizard tail regeneration while EGF and TGF-β truncate regenerative growth and commit to precocious muscle and cartilage differentiation
January 2011
Since growth factors exert control over various cellular events during development and as information in this regard on regenerative growth are scant, the present study has evaluated the effect of exogenous in loco administration of EGF, TGF-β and NGF during the first few days post-autotomy on the course of tail regeneration in Hemidactylus flaviviridis. Further evaluated is the efficacy of NGF to rectify the...
Gastrimargus musicus muscle tissue culture
January 2011
Cells from legs of Gastrimargus musicus, a grey brown grasshopper were grown in simple media. The medium was tested for growth of muscle cells and with supplements. It was found that the medium containing calcium carbonate had round cells growing close to each other in clumps. The medium containing creatine had cells growing which were elongated and branched and had all the features of fully developed muscle tissue. The...
Genistein, the phytoestrogen induces heart-and-soul (has) phenotypes in zebrafish embryo
December 2010
Zebrafish (Danio rerio) embryos treated with a small plant molecule, genistein generated a mimic of zebrafish VEGF-A loss of function model. These embryos develop with an enlarged pericardium and major blood vessel deficiencies and their morphological assessment after 48 h post fertilization indicates a nearly complete absence of both axial and intersegmental vasculature with no or reduced numbers of circulating red...
Regeneration of germlings and seedlings development from cauline leaves of Sargassum thunbergii
December 2010
A method of producing artificial Sargassum thunbergii seedlings is urgently desired to meet the increasing demand of raw materials used for aquaculture and for the sake of environmental protection. This is the first report of attempt to study the vegetative propagation of this species using cauline leaves under laboratory conditions. On average, 45.75% of the excised leaves survived and one leaf could...
Synthesis, characterization and biological activity of some novel azetidinones
June 2010
A series of seven novel azetedinones 4a-g have been synthesized by cyclocondensation of various Schiff bases of phenothiazine with chloroacetyl chloride in presence of triethylamine. Various Schiff bases of phenothiazine were synthesized by condensation of 2-hydrazinyl-1-(10H-phenothiazin-10-yl)ethanone 2 with various aryl aldehydes. Compound 2 was synthesized by reacting 2-chloro-1-(10H-phenothiazin-10-yl) ethanone 1...
Regeneration of germlings and seedlings development from cauline leaves of Sargassum thunbergii
June 2010
A method of producing artificial Sargassum thunbergii seedlings is urgently desired to meet the increasing demand of raw materials used for aquaculture and for the sake of environmental protection. This is the first report of attempt to study the vegetative propagation of this species using cauline leaves under laboratory conditions. On average, 45.75% of the excised leaves survived and one leaf could produce several...
Antibacterial activity of crude seed extracts of Buchholzia coriacea E. on some pathogenic bacteria
November 2009
The antibacterial efficacy of hot water and methanol extracts of dried seeds of Buchholzia coriacea against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, and Vibrio cholerae were determined using the Agar-gel diffusion method. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and phytochemistry of the extracts were also evaluated. Results obtained...
Photosynthetic properties of the protoplasts from Bryopsis hypnoides Lamouroux. during the early regeneration process
November 2009
The scanning electronic microphotograph (SEM), photosynthetic oxygen evolution rate and excitation spectra at liquid nitrogen temperature (77 K) of the protoplasts from Bryopsis hypnoides were determined at the early regeneration period (12 h). SEM revealed an existence of the tight aggregation; cell membrane and wall were about 10 min, 6 and 12 h respectively, after the culture. Oxygen evolution rate decreased slowly...
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