African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 414

AJBR Articles

N-nitrosodimethylamine induced changes in the activities of carcinogen-metabolizing enzymes in the liver of male mice: role of glutathione and gossypol as antioxidants

October 2007

  The importance of nutrition in protecting living organisms from the toxic effects of environmental carcinogens has attracted the attention of many researchers. In order to exert their toxic and/or carcinogenic effects, most carcinogens need to be activated primarily by phase I drug-metabolizing enzymes including; cytochrome P450, cytochrome b5, arylhydrocarbon hydroxylase (AHH), N-nitroso-dimethylamine...

Author(s): Sheweita S. A., Mousa N. and Newairy A. A.

Biochemical assessment of the effects of crude oil contaminated catfish (Clarias gariepinus) on the hepatocytes and performance of rat

October 2007

      Biochemical investigations were carried out to assess the effect of consumption of crude oil contaminated catfish on the hepatocytes and performance of rat. Catfish (Clarias gariepinus) (n = 120) were grouped into 6 of 20 catfish each and held for 30 h in 5 different mixtures of crude oil polluted water (0.1, 0.25, 0.5, 0.75 and 1% v/v). Catfish in the...

Author(s): T. O. Sunmonu and O. B. Oloyede

Physicochemical and microbiological characteristics of optimized and traditional shea butters from Côte d’Ivoire

September 2007

  A comparison was realized between the characteristics of shea butters from local markets in Côte d’Ivoire and optimized shea butters, manufactured in a laboratory, in order to valuate the improvements in quality. Large diversity concerning the colour (beige, grey and yellow), odour (odourless, moderate, fragrant and rancid) and the consistency (soft and hard) was found for the ordinary shea butter...

Author(s): Rose-Monde Megnanou , Sébastien Niamke and Jacques Diopoh

Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes

September 2007

  Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oat-spelt xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1 was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a...

Author(s): Okafor, U. A., Emezue, T. N., Okochi, V. I. , Onyegeme-Okerenta, B. M.  and Nwodo-Chinedu, S. 

Sickle-cell anaemia in Nigeria: dermatoglyphic analysis of 90 cases

September 2007

  Dermatoglyphic analysis of 90 Sickle-cell Anaemia cases and 90 normal subjects was carried out in this study.  It involved the digital patterns, ATD angle, A – B ridge count, axial triradius, digital triradius and palmar crease on the hands. 58.44% of the digital patterns in sickle-cell cases were ulnar loop as against 60.14% in the normals. The percentage of Whorl, arch and radial loop in...

Author(s): Oladipo GS, Olabiyi O, Oremosu AA, Noronha CC, Okanlawon AO and Paul CU.

Biological responses of injured human skin fibroblasts to assess the efficacy of in vitro models for cell stress studies

September 2007

  The classical central scratch method has been used to successfully induce a wound environment however this model has been criticized. This study aimed to compare the cellular responses of normal fibroblast cells with those of wounded, diabetic wounded, acidic and hypoxic cells to determine if the latter two models are more effective in inducing an injured environment than the central scratch method. The...

Author(s): Innocent Lutho Zungu, Denise Hawkins Evans, Nicolette Houreld and Heidi Abrahamse

Suppression-subtractive hybridization: A rapid and inexpensive detection methodology for up-regulated Perkinsus olseni genes

August 2007

  A cDNA forward subtraction library was constructed from the mollusc parasite Perkinsus olseni exposed to hemolymph from its natural host, the clam Ruditapes decussatus, and two different methodologies were used to unravel different non-redundant contigs. Our results demonstrated that screening of the non-enriched direct cDNA subtractive library (Dfsl) was the most efficient and least time-...

Author(s): Ascenso R. M. T., Leite R. B., Afonso R., Cancela M. L.

Analysis of phenolic compounds in Ugandan sweet potato varieties (NSP, SPK AND TZ)

August 2007

      Sweet potato varieties: Nsp, Tz and Spk obtained from Uganda were used in this study. Several phenolic compounds were identified on the basis of their mass spectra in full scan mode and the pattern of their fragmentation. The major compounds found were caffeoylquinic acid, dicaffeoylquinic acid, coumaroylquinic acid,...

Author(s): Agnes M. Nandutu, Mike Clifford and Nazlin K. Howell

Altered plasma hexose sugar metabolism in sickle cell anaemia

August 2007

  Theoretical suggestion that the metabolism and plasma levels of some hexoses (glucose and fructose) in sickle cell anaemia could be altered was investigated.  The levels were determined for 35 normal (HbAA) and for the three sickle cell states (32 HbAS; 35 HbSS, and 33 HbSS-in-crisis, HbSSc). The enzymatic glucose-oxidase method was employed in glucose estimation. The reaction of fructose with...

Author(s): Chidi G. Osuagwu and Chika U. Mbeyi

Numerical simulation of the internal vibrations of COOH group in amino-salicylic acids

July 2007

  Our present work reports the IR spectra of amino substituted salicylic acids recorded by FTIR spectrometer and also simulated theoretically. The simulation was performed using GF matrix and AM1, PM3, DFT method. In this work, the following steps were taken: optimizing the geometry, computing the IR spectra and comparing it with experimental spectra. Assuming Cspoint symmetry, vibrational assignments for the...

Author(s):   Y. P. Singh, Ratnesh Das and R. A. Singh    

Removal of iron, zinc and magnesium from polluted water samples using thioglycolic modified oil-palm fibre

July 2007

  Various kinds of agro and wood based fibres can be used to filter both trace and heavy metals from contaminated solutions. The efficacy of thioglycolic acid treated oil-palm fibre was investigated in this study. The result showed maximum absorptions (at pH = 6) of 83.6, 75.6 and 50.8% for Fe++, Zn++ and Mg++ respectively in the Southern Point (SP) samples. Similarly, 79.1, 78.3 and 77.5% for Fe++,...

Author(s): J. O. Akaninwor, M. O. Wegwu and I. U. Iba

Some valuable fatty acids exposed from wedge clam Donax cuneatus (Linnaeus)

July 2007

  The fatty acid composition of the wedge clam Donax cuneatus collected from the sandy beach of Cuddalore (southeast coast of India) was determined. In the analysis, the fatty acid profile by gas chromatography revealed the presence of higher amount of saturated fatty acids (35.28%) than mono (12.71%) and polyunsaturated (11.72%) fatty acids. Among the saturated fatty acids, the stearic acid...

Author(s): Annaian Shanmugam, Chendur Palpandi and Subramanium Sambasivam

Occurrence of malondialdehyde and N- nitrosamines and their precursors in some Nigerian ice creams, yogurts, meat and fish species

June 2007

  The malondialdehyde (a deleterious by-product of lipid peroxidation) and N-nitrosamines and their precursors (nitrate and nitrite) content of seven (7) and three (3) brands of Nigerian yogurts and ice-creams respectively as well as that of some meat and fish samples were determined using chemical methods. Malondialdehyde and nitrite were estimated spectrophotometrically, nitrate after reduction to nitrite...

Author(s): Okafor, P. N, Nwosu, O, Chukwu, J, Agbayi, J and Maduagwu, E. N

Effect of carbon sources on cellulase (EC 3. 2. 1. 4) production by Penicillium chrysogenum PCL501

June 2007

  The effects of glucose, crystalline cellulose and sawdust of Mitragyna cilata on the growth and cellulase production, inferred from cellulase (EC 3. 2. 1. 4) activity, of Penicillium chrysogenum PCL501 was determined. Glucose-containing media gave the highest mycelia weight of 1.78 mg mL-1 in 120 h of incubation. This is about 3.5 – 4.5 times the maximum weights of 0.51 and 0.40...

Author(s): Nwodo-Chinedu, S., Okochi, V. I., Smith, H. A., Okafor, U. A., Onyegeme-Okerenta, B. M. and Omidiji, O. 

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