African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 407

AJBR Articles

AQY1 gene from Debaryomyces hansenii

May 2010

Aquaporins are members of the major intrinsic protein superfamily of integral membrane proteins that enable the transport of water, glycerol and other solutes across membranes in diverse organisms. In yeasts, the proposed physiological roles of aquaporins are related to their contribution to freeze tolerance, osmoregulation and water transport. This article reports a contribution to the freeze tolerance in the...

Author(s): Juan Carlos González-Hernández

Ginger extract (Zingiber officinale) triggers apoptosis and G0/G1 cells arrest in HCT 116 and HT 29 colon cancer cell lines

May 2010

Although many studies have shown the antitumor properties of ginger extract (Zingiber officinale), little is known regarding the mechanism of its effects. This study was conducted to determine the mechanism of antitumor effects of ginger extract by evaluating apoptosis rate and cell cycle progression status in colon cancer cell lines HCT 116 and p53 defective HT 29. HCT 116 and HT 29 cells were cultured in the presence...

Author(s): Shailah Abdullah, Siti Amalina Zainal Abidin, Noor Azian Murad, Suzana Makpol, Wan Zurinah Wan Ngah and Yasmin Anum Mohd Yusof

Application of acid-hydrolyzed cassava (Manihot esculenta) and cowpea (Vigna unguiculata) for the production of yeast (Saccharomyces cerevisiae)

April 2010

Much progress has been made in the cultivation and production of cassava (Manihot esculenta) and cowpea (Vigna unguiculata) in Nigeria. In the present study, investigation was carried out on the possibility of using cassava flour as a source of glucose as well as cowpea as source of nitrogen in the production of yeast. Acid hydrolysis (using dilute H2SO4) of cassava and cowpea was undertaken to release the sugars and...

Author(s): Nyerhovwo J. Tonukari and Linda I. Osumah

Captopril interferes with some serum biochemical findings

April 2010

Captopril is a widely used anti-hypertensive drug that acts by inhibiting angiotensin-converting enzyme. This work has been carried out to investigate the effects of captopril on some common biochemical laboratory parametres in the sera of patients receiving the drug. For this study, 40 subjects were included, all within the age range of 40 to 63 years and with newly diagnosed essential hypertension. From each patient,...

Author(s): I. A. Ibrahim, F. S. Al-Joudi , R.  Waleed Sulaiman  and B. Hilal AL-Saffar

Effect of ferric oxide nanoparticles on microtubules organization

April 2010

Nanoparticles (NPs) are widely used in several manufactured products. The small size of nanoparticles facilitates­ their uptake into cells as well as transcytosis across epithelial cells into blood and lymph circulation to reach different sites, such as the central nervous system. Studies have shown different risks of Fe2O3NPs in the neuronal system and other organs. They are membrane-bound layer aggregates or...

Author(s): Ali Khaleghian, Gholam Hossein Riazi, Shahin Ahmadian, Mahmoud Ghafari, Marzieh Rezaie, Akira Takahashi, Yutaka Nakaya and Hossein Nazari

Synergistic effects of glucan and resveratrol

April 2010

Recent data showing that glucan stimulates defense reactions in plants through synthesis of resveratrol, led us to study the possible synergetic effects of a glucan-resveratrol complex on immune reactions in mice. We measured phagocytic activity, expression of CD4 marker on spleen cells, IL-2 secretion and antibody response. In all cases we confirmed the stimulatory effects of glucan. Resveratrol alone had either limit...

Author(s): Vaclav Vetvicka and Zuzana Vancikova

Assessing the role of RecA protein in the radioresistant bacterium, Deinococcus geothermalis

April 2010

The moderately thermophilic bacterium Deinococcus geothermalis exhibits extraordinary resistance to ionizing radiation. RecA protein is considered to be one of the most important participants in radioresistance. To assess the role of the RecA protein in D. geothermalis, the recA gene was isolated from D. geothermalisand over expressed in Escherichia coli. After the D. geothermalis RecA...

Author(s): Haïtham Sghaier, Katsuya Satoh, , Hirofumi Ohba,  and Issay Narumi,

Fermentation in cassava (Manihot esculenta Crantz) pulp juice improves nutritive value of cassava peel

March 2010

A major challenge in using cassava peel as feed for animals is the presence of cyanogenic glycosides and the low concentration of protein. The present study investigated the possibility of upgrading cassava peels using fermented cassava pulp juice. Cassava pulp juice was squeezed out of grated cassava pulp and fermented for 3 days at ambient temperature. The microorganisms in the fermented pulp juice were identified...

Author(s): Naa Ayikailey Adamafio, Maxwell Sakyiamah and Josephyne Tettey

Production of cuticle - degrading proteases by Beauveria bassiana and their induction in different media

March 2010

The concern for the development of hyphomycete fungi as suitable biocontrol agents of insect pests leads to the isolation of various insect pathogenic fungi. Amongst them, one of the most studied entomopathogenic fungus is Beauveria bassiana. The conidia of mitosporic fungi adhere to the host cuticle and germinate to produce an infectious propagule, and produce a sequential release of extra cellular enzymes to...

Author(s): Priyanka Dhar and Gurvinder Kaur

Optimization of culture parameters to enhance production of amylase and protease from Aspergillus awamori in a single fermentation

March 2010

Concomitant production of amylase and protease by Aspergillus awamorinakazawa MTCC 6652 was enhanced in a single fermentation by media engineering and optimization of other important parameters. Wheat bran was considered as a suitable substrate for production of amylase and protease by A. awamori in a single fermentation as none of the additional substrate such as powder of peanut, corn, soybean seeds and...

Author(s): Sangeeta Negi and Rintu Banerjee

Hepatoprotective role of Garcinia kolanut (Heckel) extract on methamphetamine: Induced neurotoxicity in mice

March 2010

The hepatoprotective effect of aqueous extract of Garcinia kola (AEGK) was studied in 60 mice of mixed sexes. The animals were divided into 6 groups of 10 mice each. Group I received normal saline, Groups II and III got 100 and 200 mg/kg AEGK (orally), respectively. Group IV received 10 mg/kg methamphetamine (MAM) (s.c.) only. Groups V and VI got 100 and 200 mg/kg of AEGK respectively, before 10 mg/kg methamphetamine...

Author(s): Gabriel Oze, Iheanyi Okoro, Austin Obi and Polycarp Nwoha

Lecithin: Cholesterol acyltransferase, lipoprotein lipase and lipoproteins in adult Nigerians with sickle cell disease

February 2010

Lecithin: Cholesterol acyltransferase (LCAT) is a glycoprotein enzyme involves in the esterification of plasma cholesterol to form cholesteryl ester and is important in the maturation of high density lipoprotein. Lipid metabolism in sickle cell anaemia patients may be affected. The objective of this study was to determine the activities of serum lecithin: cholesterol acyltransferase, lipoprotein lipase, lipids and...

Author(s): M. A. Emokpae, , P. O. Uadia and H. B. Osadolor

Toxic effect of lead on kidney function in rat wistar

February 2010

Lead is one of the environmental pollutants that can threat the life of living creatures in many ways. This study was carried out to investigate the effect of lead exposure on kidney function. Fourteen male Wistar rats were divided into 2 groups; group 1 was given a tap water diet and group 2 was given 1000 ppm lead acetate in drinking water for 8 weeks. Lead concentration in blood was determined by atomic absorption....

Author(s): Missoun F, Slimani M and Aoues A

Changes in haemorrheologic and fibrinolytic activities upon hypertension and diabetic chemotherapy in Calabar diabetic residents, Nigeria

February 2010

This research was carried out to assess the effect of hypertension on haemorrheologic and fibrinolytic activities in fifty (50) diabetics resident in Calabar municipality and the values obtained were compared with those of fifty (50) age and sex-matched non diabetics in the same locality. Relative plasma viscosity plasma fibrinogen concentration, euglobulin lysis time and fasting blood sugar were determined using...

Author(s): M. S. Edem, A. O. Emeribe and J. O. Akpotuzor

The response to iron deficiency of two sensitive grapevine cultivars grafted on a tolerant rootstock

February 2010

Two sensitive cultivars were examined, Vittoria and Italia, grafted on a tolerant rootstock (140 Ruggeri). Two levels of iron chlorosis in scions were selected, initial and evident, and compared to the healthy rootstock (control). The fractions of extracellular and cytoplasmatic cations, chloroplastic mobile, loosely linked, strongly linked and residual cations as well as the active fraction were...

Author(s): M. A. Russo, F. Sambuco and A. Belligno

The effects of aqueous extracts of the leaves of Hibiscus rosa-sinensis Linn on renal function in hypertensive rats

February 2010

The leaves of Hibiscus rosa-sinensis Linn (Family Malvaceae) have been used in ethnomedicine for the treatment of various human diseases such as aphrodisiac, hypertension, wound healing, diabetes mellitus and cancer. In this present study, the effect of 200 mg/kg of the aqueous leaf extract on the renal function of hypertensive rats was investigated. The administration of H. rosa-sinensis leaves...

Author(s): Imafidon E. Kate and Okunrobo O. Lucky

Effects of soaking and boiling and autoclaving on nutritional quality of Mucuna flagellipes (Ukpo)

February 2010

The effect of soaking and boiling, and autoclaving on nutritional factors (proximate and mineral) and anti-nutritional qualities of legume, Mucuna flagellipes were studied. Batches of seeds were soaked for 6, 12, 18 and 24 h in distilled water at room temperature, then boiled in water for 30, 45, 60 or 90 min respectively. Another batch of the M. flagellipes was autoclaved for different duration of...

Author(s): E. A. Udensi, N. U. Arisa and E. Ikpa

The influence of 950 MHz magnetic field (mobile phone radiation) on sex organ and adrenal functions of male rabbits

February 2010

Mobile phones are used frequently and held close to the body thus they are potentially dangerous sources of electromagnetic (EM) radiations. The clear effects of EMF (electromagnetic field) on many organs of the body have not been known until now and have puzzled scientists. The objective of this study was the evaluation of the influence of different powers (3 and 6 W) of 950 MHz magnetic field on testosterone, FSH...

Author(s): M. R. Sarookhani, M. Asiabanha Rezaei, A. Safari, V. Zaroushani and M. Ziaeiha

Effect of egusi melon oil on lecithin: Cholesterol acyltransferase activity in rats fed a cholesterol diet

January 2010

The effect of feeding egusi melon oil as a supplement to a cholesterol-based diet on serum lecithin: cholesterol acyltransferase activity of rats was evaluated. The rats were divided into two groups designated: control and test respectively. Rats in the test group were fed 5% cholesterol diet supplemented with 5% egusi melon oil while the control rats received 5% cholesterol diet...

Author(s): G. C. Ojieh, G. O. Eidangbe and O. M. Oluba

Assessment of current iodine status of pregnant women in a suburban area of Imo State Nigeria, twelve years after universal salt iodization

January 2010

Three hundred and two pregnant women participated in this study. Our results showed that the range, mean and median urinary iodine excretion (UIE) were 28.1 to 218.1, 152.09 ± 41.65 and 163.1 µg/l, respectively. The range, mean and median TSH concentration were 0.7 - 5.9, 1.4 ± 0.7 and 1.3 µIU/ml, respectively. Our results showed that none of the women have severe iodine deficiency, 2% had...

Author(s): Cosmas O. Ujowundu, Agwu I. Ukoha, Comfort N. Agha, Ngwu Nwachukwu and Kalu O. Igwe

A study on prostate specific antigen (PSA) with the ratio of free to total PSA

January 2010

Prostate Specific Antigen (PSA) is a glycoprotein produced as a marker by prostate gland for prostate cancer, benign prostate hyperplasia and prostatitis. Study objective is to evaluate f/t PSA ratio to distinguish the Benign Prostate Hyperplasia (BPH) and prostate cancer patients in and around SRM University. To define the age specific reference ranges of PSA in control and test group at Chennai, India. Healthy men...

Author(s): Chavan Atish, Suman Diggi Prasad, Rawal Mukesh, Kandasamy Surendran, Sivapatham Sundaresan, Thangarajan Thangapannerselvem

Cytoplasmic, peroxisomal and mitochondrial membrane phospholipid alteration in 31-diamino-azo-benzene (31-DAB)-induced hepatocellular carcinoma

January 2010

Alteration in the cytoplasmic, mitochondrial and peroxisomal membrane phospholipid has been investigated in 31- diamino-azo-benzene (DAB) -induced hepatocellular carcinoma in rats. The cytoplasmic membrane of tumour group shows 27.5, 19.7, 38.7 and 34.7% increase in phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin respectively compared with the control group, mitochondrial membrane...

Author(s): Omotuyi I. O., Ologundudu A., Okugbo T. O., Oluyemi K. A. and Omitade, O. E.

Investigation of antimicrobial and plasma coagulation property of some molluscan ink extracts: Gastropods and cephalopods

January 2010

The ink secretion of molluscan species was identified as one of the novel sources of bioactive compounds. Aqueous ink extracts of Dollabella auricularia inhibited the growth of Gram-positive and Gram-negative bacteria. In addition to D. auricularia, Octopus vulgaris and Sepia aculeate ink samples showed antifungal activity. The antimicrobial activities of the samples were not due to the...

Author(s): R. Vennila, R. K. Rajesh kumar, S. Kanchana, M. Arumugam and T. Balasubramanian

Anti-inflammatory and anti-ulcerogenic activity of the ethanol extract of ginger (Zingiber officinale)

December 2009

  The acute toxicity test carried out on the ginger extract gave the LD50 value as 1000 mg/kg. The antiinflammatory and anti-ulcerogenic effects of the ethanol extract of ginger (Zingiber officinale) in adult Wistar rats were studied using values below the lethal dose. Inflammation was induced by injecting 0.1 ml undiluted fresh egg albumin (philogistic agent) into the subplantar surface of the...

Author(s): Chioma A. Anosike, Onyechi Obidoa, Lawrence U. S. Ezeanyika and Meshach M. Nwuba.

Modulation of melanin synthesis and its gene expression in skin melanocytes by palm tocotrienol rich fraction

December 2009

  Melanin is the pigment that determines skin color. Melanin synthesis is catalysed by the enzyme tyrosinase and is controlled by TYR, TYRP1 and TYRP2 genes. The objective of this study was to evaluate the anti pigmentation property of palm tocotrienol rich fraction by determining melanin synthesis and expression of genes involved in its regulation in skin melanocytes. Palm tocotrienol...

Author(s):   Suzana Makpol, Nur Nadia Mohd Arifin, Zahariah Ismail, Chua Kien Hui, Yasmin Anum Mohd Yusof and Wan Zurinah Wan Ngah   

Antifeedant and antimicrobial activity of Tylophora indica

December 2009

  Crude and pure extracts of Tylophora indica were investigated in view of antifeedant, antibacterial and antifungal properties.  Leaf crude extract showed more antifeedant activity than stem and root against Spodoptera litura, a polyphagous pest on wide ranging crops. Among the pure compounds isolated, Tylophorine showed the highest antifeedant activity followed by Septicine, O-Methyl...

Author(s):   B. Krishna Reddy, M. Balaji, P. Uma Reddy, G. Sailaja, K. Vaidyanath and G. Narasimha

Effect of falciparum malaria on some plasma proteins in males: With special reference to the levels of testosterone and cortisol

November 2009

  Sex-associated hormones were evidenced to modulate immune responses and consequently directly influence the outcome of infection. Testosterone is known to influence both protein metabolism and the level of cortisol which is the hormone of stress. This work was conducted to explore the influence of the degree of parasitemia in Plasmodium falciparum malaria on the male sex hormone testosterone, plasma...

Author(s): Muawia A. Abdagalil and Nabiela M. ElBagir

Immunopurification of a rape (Brassica napus L.) seedling lipase

November 2009

  Lipase or triacylglycerol acylhydrolase (E.C.3.1.1.3) was purified to homogeneity from rapeseed-germinated cotyledons (Brassica napus L.). The purification scheme involved homogenization, centrifugation, ultracentrifugation and affinity chromatography using polyclonal antibodies raised against porcine pancreatic lipase. The purified rapeseed lipase was homogenous and did not contain...

Author(s): H. Belguith, S. Fattouch, T. Jridi and J. Ben Hamida

Effect of Gongronema latifolium crude leaf extract on some cardiac enzymes of alloxan-induced diabetic rats

November 2009

  The effect of Gongronema latifolium on serum cardiac enzymes in alloxan induced diabetic rat models and normal control rats were determined using graded doses of 80% ethanol extract of G. latifolium leaf. The serum activities of aspartate aminotransferase (AST), creatine kinase (CK), creatine kinase isoenzyme (CKMB) and lactate dehydrogenase (LD) were determined in diabetic Wistar albino...

Author(s): E. E. Edet, M. I. Akpanabiatu A. E. Eno, I. B. Umoh and E. H. Itam

Electrolyte profile and prevalent causes of sickle cell crisis in Enugu, Nigeria

November 2009

  One hundred sickle cell patients aged between 6 - 25 years in steady state who attended Sickle Cell Clinic at University of Nigeria Teaching Hospital, Enugu, Nigeria were selected for this study. Out of this number, only thirty who were eventually admitted in crisis state within one year of this study were selected for subsequent investigations. They included 20 females and 10 males. We also selected thirty...

Author(s): E. O. Ibe, A. C. J. Ezeoke, I. Emeodi, E. I. Akubugwo, E. Elekwa, M. C. Ugonabo and W. C. Ugbajah

The role of ascorbic acid in the treatment of Plasmodium Berghei infected mice

November 2009

  This work aimed at examining the effect of malaria parasites and ascorbic treatments in mice. The relevance of this research derives from the desire to understand the role of ascorbic acid in malaria infection. In this study design, three groups of ten mice each categorized as non-parasitized-non-treated (control), parasitized-non-treated (PnT) and parasitized ascorbic acid treated (P+asT) were used....

Author(s): H. O. T. Iyawe and A. O. Onigbinde

Prevention of cadmium-induced alteration in rat testes and prostate lipid patterns by α-tocopherol

October 2009

Cadmium induced testicular damage has been investigated in α-tocopherol (Vitamin E) pretreated and non-pretreated male rats exposed to a single sub-lethal dose of cadmium in form of CdCl2. Graded doses of vitamin E (75, 150, and 750 mg kg-1 body wt.) were administered daily to rats in separate groups by gavage for 4 weeks while 3 mg Cd kg-1 body wt was administered subcutaneously, 24 hr to the termination of the...

Author(s): M. A. Adaikpoh and F. O. Obi

The hormone-emotion-behavioural gene-neuromessenger labyrinth: Pertinent questions

October 2009

  The discovery of behavioural genes has raised the prospect that behaviour may be governed, in part, by the actions of mutant gene products on the brain. These are mostly enzymes or proteins involved in processes related to neurotransmission. In addition, numerous studies have demonstrated the effects of specific hormones on behaviour. Furthermore, the ability of intense emotions to stimulate or suppress the...

Author(s): N. A. Adamafio

Determination of toxic metal concentrations in flame-treated meat products, ponmo

October 2009

  Cowhides ‘ponmo’ prepared by various and unknown processing methods were purchased from several markets in Lagos and environs and the contents of lead, cadmium, chromium, zinc, mercury and arsenic were determined before and after boiling. Significantly high levels of these metals except cadmium and zinc were found in all the samples obtained after boiling. The highest levels of Pb (1.54 mg.kg-1),...

Author(s): W. Okiei, M. Ogunlesi, F. Alabi, B. Osiughwu and A. Sojinrin

Studies on separation techniques of pomegranate seeds and their effect on quality of Anardana

October 2009

  The dehydrated seeds of wild pomegranate fruits (Anardana) are used as acidulent in culinary preparations and in making various medicines. For easy separation of seeds, fruits were subjected to sand roasting, hot water dipping and soaking in cold water. Hot water dip for 2 min saved time required for separation of seeds from fruits (80% excluding treatment time and 74% including treatment time). Due to the...

Author(s): Amit Parashar, S. K. Gupta and Ashok Kumar

Antidermatophytic activities of Ixora brachiata Roxb

October 2009

  The in vitro antifungal activity of Ixora brachiata Roxb. leaf and root extracts were evaluated against three different genera of dermatophytes viz. Microsporum,Trichophyton and Epidermophyton by Dilution Agar Method. The crude extracts ofIxora brachiata root and leaf showed in-vitro antifungal properties and completely prevented the growth of tested...

Author(s): B. Sadeghi-Nejad and S. S Deokule

Recent advances in the characterization of peptidyl transferase center: Zero-distance labeling of proteins at or near the catalytic site of human 80S or Escherichia coli 70S ribosomes by means of periodate-oxidized tRNA

September 2009

  Periodate-oxidized tRNA (tRNAox), the 2’,3’-dialdehyde derivative of tRNA, was used as a zero-length active site-directed affinity labeling reagent, to covalently label proteins at the peptidyl transferase center (PTC), the catalytic site of the large ribosomal subunit. When human 80S or E. coli 70S ribosomes were reacted separately with tRNAox positioned at the P-site, in the...

Author(s): Soria Baouz, Anne Woisard, Lila Chenoune, Gustave Aguié, Gérard Keith, Jean-Marie Schmitter, Jean-Pierre Le Caer and Codjo Hountondji

Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides

September 2009

  Tank-raised Clarias gariepinus (mean total length, 30.05±4.05cm,SD; mean weights, 250.04±50.22gSD) were injected intra-muscularly with two millilitres per kilogram of each of five concentrations (2.00, 2.00, 6.00, 8.00 and 10.00ppm) of aqueous extracts of leaves of Lepidagathis alopecuroides above the lateral line. The fish in the control group were injected with same dose of...

Author(s): U. U. Gabriel, F. G. Obomanu and O. S. Edori

Levels of cadmium in different brands of cigerettes sold in Abakaliki metropolis of Nigeria

September 2009

  Cadmium (Cd) is recognized as category 1 carcinogen by the international agency for research on cancer based on its relationship to pulmonary tumors. Cigarette smoking is one major source of Cd in the environment. Levels of Cd in 19 different brands of cigarettes (both local and foreign made) sold in Abakaliki town were determined using atomic absorption spectrophotometer (AAS) (205). The results showed...

Author(s): Augustine A. Uwakwe and Udu Ibiam

Predicting a-amylase yield and malt quality of some sprouting cereals using 2nd order polynomial model

August 2009

  Alpha amylase yield in sprouting Maize, Acha, Rice and Sorghum were studied for 180 h. The result was analyzed using 2nd order polynomial model. The result showed that the rate of α- amylase secretion with growth period is significantly high (p < 0.05) and the R2 for each ranged from 67 - 90%, while the R2 for sprouting vigour ranged within 99% for all the cereals studied. The...

Author(s): C. Egwim Evans and O. Adenomon Monday

Asymmetrical dimethylarginine (ADMA) and nitric oxide as potential cardiovascular risk factors in type 2 diabetes mellitus

August 2009

  Hyperglycemia affects biochemical parameters and influences the progression of coronary heart disease and mortality rates in diabetic patients. L-arginine is the substrate used by NO synthase to produce the vasodilator NO. However, in patients with type 2 diabetes mellitus (T2DM), there is an increase in serum levels of methylated L-arginines, such as ADMA, which is a recently identified potent cardiovascular...

Author(s): Mohamed H. Mahfouz, Ibrahim A. Emara, Mohamed S. Shouman and Magda K. Ezz 

Toxicological studies of Ceiba pentandra linn

July 2009

  The toxicity profile of aqueous methanol extract of Ceiba pentandra leaves was investigated in mice. Two parameters were determined; acute toxicity and chronic toxicity. For acute toxicity test, the LD50 was found to be over 5000 mg/kg. For chronic toxicity test, the mice were injected with graded doses (250 - 500 mg/kg) of the leaves extract daily for 21 days and the effects on body...

Author(s): S, Sarkiyayi, S. Ibrahim and M. S. Abubakar

Influence of road transportation during hot summer conditions on oxidative status biomarkers in Iranian dromedary camels (Camelus dromedarius)

July 2009

  Transportation causes stress in livestock that may alter numerous physiological variables with a negative impact on production and health. The objective of the current study was to investigate the effects of road transport on oxidative stress biomarkers in camels. Ten Iranian dromedary camels were selected and subjected to a journey of approximately 300 km in a truck by road in August 2008. Blood samples were...

Author(s): Saeed Nazifi, Mahdi Saeb, Hasan Baghshani and Saeedeh Saeb

Comparative methaemoglobin concentrations of three erythrocyte genotypes (HbAA, HbAS and HbSS) of male participants administered with five antimalarial drugs

June 2009

  In the present in-vivo study, the capacities of five antimalarial drugs (Fansidar, Halfan, Quinine, Coartem and Chloroquine phosphate) to alter/distort methaemoglobin concentrations of three human erythrocyte genotypes (HbAA, HbAS and HbSS) was investigated. Spectrophotometric method was used to ascertain this erythrocyte parameter. The male participants enrolled for this study were grouped...

Author(s): P.C. Chikezie

Purification of a mannose/glucose-specific lectin with antifungal activity from pepper seeds (Capsicum annuum)

June 2009

  A lectin was isolated and purified from the pepper seeds Capsicum annuum. The purification procedure involved anionic exchange chromatography on DEAE – Cellulose and QAE – Sephadex columns followed by gel filtration on Sephadex G–100. The heamagglutinating activity of the lectin towards human erythrocytes was sensitive to inhibition by D-mannose and D-glucose; and enhancement by...

Author(s): Adenike Kuku, Oludele Odekanyin, Kemi Adeniran, Mary Adewusi and Toyin Olonade

The Hsp40-Hsp70 chaperone machinery of Plasmodium falciparum

May 2009

  Plasmodium falciparum is the protozoan parasite responsible for the most virulent form of malaria. The majority of the asexual stages of its life cycle occur in the human erythrocyte. Since the infected erythrocytes undergo dramatic structural and functional changes upon parasite infection, malaria research has been focusing on investigating the proteins potentially involved in host cell...

Author(s): Eva-Rachele Pesce and Gregory L. Blatch

The use of circular dichroism spectroscopy to study protein folding, form and function

May 2009

Circular Dichroism (CD) is a spectroscopic technique widely used for the evaluation of the conformation and stability of proteins in several environmental conditions like temperature, ionic strength, and presence of solutes or small molecules. Circular Dichroism spectroscopy is non-destructive, relatively easy to operate, requires small amount of sample and few data collection. Additionally, data analyses are fast....

Author(s): Daniel H. A. Corrêa,  and Carlos H. I. Ramos

A novel antifungal phenolic compound from Parrotia persica

May 2009

  Aqueous and methanol extracts of Parrotia persica leaves were assayed for antifungal activity against phytopathogenic Fusarium oxysporum and human pathogenic Candida albicans by poisoned food technique. Both the aqueous and methanol extracts demonstrated significant antifungal activity. Further fractionation of methanol extract guided by antifungal activity resulted in the...

Author(s): M. Ahanjan, M. P. Raghavendra, and K. A. Raveesha

Effects of cinnamon on blood glucose and lipids levels in diabetic patients (Type1)

May 2009

  The present study was designed to investigate the effects of supplementation of cinnamon on blood glucose and lipids levels among type1 diabetics. The samples consisted of 60 subjects with type1 diabetes and the doses of cinnamon were equally administered orally in the form of capsules with breakfast, lunch and dinner. The doses were given for 4 weeks. Blood samples were taken on the starting day of the...

Author(s): Abdul Rahim Al Jamal

Salivary amino acids quantification using RP-HPLC during normal menstrual cycle

May 2009

  The present study was carried out to detect amino acids profile in women saliva in order to establish the qualitative and quantitative differences that might have potential value in detection of ovulation by noninvasive methods. For the collection of sample, the stages of menstrual cycle were decided by the physical and morphological examination of salivary fern pattern. The saliva from various reproductive...

Author(s): S. Alagendran, K. Rameshkumar, K. Palanivelu, N. Puspha, M. Ranjani, N. Arulmozhi and G. Archunan

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