African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 406

AJBR Articles

Thermostable, alkaline tolerant lipase from Bacillus licheniformis using peanut oil cake as a substrate

June 2011

Thermostable alkaline tolerant lipase producing Bacillus licheniformis isolated from marine environment. Peanut oil cake was used as substrate for enzyme production and purified to 3.6 fold with a specific activity of 148.4 U/mg of protein with molecular weight of 35 kDa. The enzyme was 100% stable at 60°C, it retains more than 80% activity after 1 h incubation at 70°C and pH 9-11.5. Metals like Ca2+,...

Author(s): N. Annamalai, S. Elayaraja, S. Vijayalakshmi and T. Balasubramanian

Changes in the amino acids contents of selected leafy vegetables subjected to different processing treatments

June 2011

Changes in the amino acids contents of Veronica amygdaline, Gnetum africana, Gongronema latifolium and Ocimum gratissimum subjected to different processing treatments were investigated. The processing treatments employed include fresh milling, sun drying, oven drying, steaming and a combination of these while the amino acids evaluated include isoleucine, leucine, lysine, methionine, cysteine,...

Author(s): Chinyere, G. C. and Obasi, N. A.

a1–Antitrypsin: Anti-inflammatory roles in exercise and atherosclerosis

May 2011

a1-Antitrypsin, also known as serum trypsin inhibitor, is an acute phase protein that is upregulated in response to tissue damage and infection. More specifically this glycoprotein affords the host protection against enzymes that are released by immune inflammatory cells. The most notable of these enzymes is neutrophil elastase. Neutrophil elastase has the ability to damage vasculature and in doing so may contribute to...

Author(s): Stuart .J. Semple and Andrew .J. McKune

Variation in antioxidant and aroma compounds at different altitude: A study on tea (Camellia sinensis L. Kuntze) clones of Darjeeling and Assam, India

May 2011

Plants at high altitude are subject to enhanced oxidative stress due to high UV fluence (resulting from air rarefaction) compared to places at low altitude. For survival, plants have developed specific cell protective compounds viz. the flavonoids. Flavonoid derivatives (viz. flavonoid glycoside) may also serve as precursor of aroma producing compounds and are thus relevant in studies on plant quality under varying UV...

Author(s): Sudeshna Bhattacharya and Swati Sen-Mandi

The effects of mannan-oligosaccharides on cecal microbial populations, blood parameters, immune response and performance of broiler chicks under controlled condition

May 2011

A study was conducted to evaluate the effects of mannan-oligosaccharides (MOS) on cecal microbial populations, immune responses to phytohaemagglutinin-P (PHA-P) and sheep red blood cell (SRBC) and performance (weight gain and feed conversion ratio) of broiler chicks under strict controlled condition. Sixty four day-old male broiler chicks were randomly assigned to 12 battery cages pens of 4 chicks each and were fed...

Author(s): Saeed Khalaji, Mojtaba Zaghari and Somaye Nezafati

Radio-protective chelating agents against DNA oxidative damage

April 2011

The present study aims to investigate the strong neuro and radio-protective activity of chelating agents through inhibition of metal catalyzed Fenton reaction and aims to explore the potential of PCR-RFLP as biomarkers. Polymorphisms of rat brain angiotensin II subtype 2 receptor gene (AT2RG) were investigated as a biomarker for the effect of 6 Gy irradiation with or without prior carnosine and dimer­captosuccinic...

Author(s): M. H. Awwad, Samy A. Abd El-Azim, F. A. M. Marzouk, E. A. El-Ghany, and M. A. Barakat

Multipotent mesenchymal stem cells (MSCs) from human umbilical cord: Potential differentiation of germ cells

April 2011

Previous controversy exists as to whether umbilical cord (UC) can serve as a source of multipotent mesenchymal stem cells (MSCs) with their characteristics. Different methods and reagents have been used to induce the differentiation of UC-MSCs into functional cells. We investigated the isolation of UC-MSCs and their potential differentiation into neurons, cardiomyocytes and germ cells in vitro. The phenotypes,...

Author(s): Jinlian Hua, Pubin Qiu, Haijing Zhu, Hui Cao, Fang Wang, and Wei Li

Comparison of peroxidase activities from Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in Burkina Faso

April 2011

Current applications of peroxidase in various areas of biotechnology and clinical biochemistry show the interest for further screening for peroxidase. Thus, peroxidase activities were screened in higher plants such as Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in a tropical environment. The enzymes were investigated for their specific activities and best...

Author(s): Mamounata Diao, Oumou H. Kone, Nafissétou Ouedraogo, Romaric G. Bayili, Imael H. N. Bassole, and Mamoudou H. Dicko

Levels of two oxidative stress indicators of human sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa

April 2011

The present in vitro study investigated the levels of oxidative stress indicators, namely, malondialdehyde (MDA) and methaemoglobin (mHb) when sickle erythrocytes were incubated in aqueous leaf extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa for 12 h. At regular time intervals of 3 h, portions of the incubation mixture were withdrawn and spectrophotometric...

Author(s): Paul Chidoka Chikezie

Utilization of dietary therapies in the alleviation of protein energy malnutrition in kwashiokor-induced rats

April 2011

The utilization of diets prepared from available and affordable plants (soya bean, groundnut, maize, and fluted pumpkin green leaves) and animal sources (milk, catfish, and crayfish), in the alleviation of protein energy malnutrition (PEM) was studied, to determine comparative dietary efficiencies of some fortified weaning formulae. The therapeutic diets used in alleviating PEM in the kwashiorkor-induced rats were...

Author(s): Obimba, Kelechukwu Clarence

Incomplete laboratory request forms as a contributory factor to preanalytical errors in a Nigerian teaching hospital

March 2011

That most laboratory errors occur in the preanalytical phase has been demonstrated in the past. There is paucity of data on the contribution of incomplete request forms to preanalytical errors in Nigerian hospitals. We examined our laboratory request forms for the frequency of incomplete data. Request forms received at the laboratory in a three- month period were examined for some parameters. The completeness of...

Author(s): Adegoke O. A., Idowu A. A. and Jeje O. A.

Activities of some enzymes, enzyme inhibitors and antinutritional factors from the seeds of sponge gourd (Luffa aegyptiaca M.)

March 2011

Activities of some of the enzymes; enzyme inhibitors and antinutritional factors of the seeds ofLuffa aegyptiaca were investigated. Enzymes were extracted and their activities determined using standard procedures. The activities of trypsin and amylase inhibitors were also determined using standard procedures. While antinutritional factors were detemined using the AOAC method as well as standard procedures. The...

Author(s): Gloria N. Elemo, Babajide O. Elemo and Ochuko L. Erukainure

Extraction and application of dye extracted from eriophyid leaf galls of Quercus leucotrichophora-A Himalayan bluejack oak

March 2011

Oaks (Quercus sp.) are susceptible to a wide diversity of gall-forming insects. When the insect pupates and leaves, the gall can be used as a dyestuff. Kalamkari unit’s method (India) was followed for the extraction of dye. A dark brown dye was obtained from the leaf gall of Quercus leucotrichophora caused by Eriophyes sp. Three different types of cloths and three different types of yarns...

Author(s): Preeti Mishra and Vidya Patni

Characterization of the polysaccharide material that is isolated from the fruit of Cordia abyssinica

March 2011

Treatment of aqueous extracts of the fruit of Cordia abyssinica, containing 0.25% sodium chloride with three volumes of ethanol produced an acidic polysaccharide with a 2% yield, on a fresh weight basis. Upon precipitation of the polysaccharide with acid, the yield of the polysaccharide decreased to 1.2% and the polymer showed some level of degradation. With protein content between 2.6 and 4.6% for the acid and...

Author(s): Benhura, M. A. N. and Chidewe, C.

Trace metals and oxidative metabolic changes in malignant prostate cancer patients

March 2011

Metabolic derangement commonly associated with prostate cancer (PCa) has not been well researched in Nigerian malignant PCa patients yet. The present study was designed to assess the levels of selected trace metals (Zn, Cu, Cr, Fe, Co, and Se) and markers of oxidative stress,such as total plasma peroxide (TPP), total antioxidant potential (TAP), oxidative stress index (OSI) and free malondialdehyde...

Author(s): Akiibinu M. O., Ogundahunsi A. O., Kareem OI, Adesiyan A. A., Idonije B. O. and Adeniyi F. A. A.

Molecular and biochemical novel cancer biomarker for early detection of breast cancer

March 2011

Publisher's Retraction

Author(s): Patil V. W., Rajeev S. and Patil A. V.

Polymerization of human sickle cell haemoglobin (HbS) in the presence of three antimalarial drugs

February 2011

To study the capacity of antimalarial drugs to interfere with polymerization of sickle cell haemoglobin (HbS), in vitro polymerization was induced by incubating erythrocyte haemolysate of HbS with 2 g% sodium metabisulphite (Na2S2O5) in the presence of 2 mg% concentration of three (3) separate antimalarial drugs (CoartemTM, Quinine, and Chloroquine phosphate). Spectrophotometric method was used to ascertain...

Author(s): Chikezie P. C., Chikezie C. M. and Amaragbulem P. I.

Purification and biochemical characterization of a xylanase purified from a crude enzyme extract for the determination of active site residues

February 2011

This paper describes the purification of a thermostable xylanase (Xyn) from a commercially available xylanase stock (Buzyme 2511 from Buckman Laboratories International, South Africa).The purified enzyme showed a Mr. of 66 kDa, and presented a dimeric form with two similar molecular weight chains of 33 kDa. The optimum pH and temperature were found to be 6.0 and 50°C, respectively. The enzyme was active over a broad...

Author(s): Sacha Tabosa-Vaz, Ajit Kumar, Kugen Permaul and Suren Singh

Iodine and inorganic mineral contents of some vegetables, spices and grains consumed in Southeastern Nigeria

February 2011

Selected vegetables, spices and grains which serve as staples in Southeastern Nigeria were analyzed for their iodine and some essential minerals. Our results showed that the mean iodine concentration of the vegetables were of 94.61 ± 15.28 µg/100 g, with Uha leaves (Pterocarpusspp.) having the highest with 117.66 ± 3.00 µg/100 g iodine, cowpea (Vigna...

Author(s): Ujowundu C. O., Kalu F. N., Nwosunjoku E. C., Nwaoguikpe R. N., Okechukwu R. I. and Igwe K. O.

Iodine nutrition for pregnant women in three major villages of Ohafia local government area in Abia State of Nigeria

February 2011

Median urinary iodine concentrations of three hundred pregnant women in three major villages in Ohafia Local Government Area of Abia State, South-Eastern Nigeria were determined. This is to help determined whether iodine nutrition was sufficient or not in pregnant women. The analysis of iodine in urine was by spectrophotometric reaction in which specimens were manually digested with ammonium persulfate. The median...

Author(s): Igwe K. O., Ukoha A. I., NwaChukwu N. and Ujowundu C. O.

In vitro biodegradation of keratin by dermatophytes and some soil keratinophiles

January 2011

The present investigation was aimed to evaluate the in vitro biodegradation of keratin by clinical isolates of dermatophytes and soil fungi. Ten fungal species, out of which, six (Chrysosporiumindicum, Trichophyton mentagrophytes, Scopulariopsis sp., Aspergillus terreus, Microsporumgypseum and Fusarium oxysporum) were isolated from soil and four clinical...

Author(s): Mukesh Sharma, Meenakshi Sharma and Vijay Mohan Rao

ACTN3 and TNF gene polymorphism association with C-reactive protein, uric acid, lactate and physical characteristics in young African cricket players

January 2011

The genotyping of α-actinin-3 (ACTN3) and tumor necrosis factor-α (TNF) genes of 31 Zulu South African males aged 20 to 27 years (14 cricketers and 17 controls) was performed by PCR amplification followed by restriction digestion. After ANOVA the associations with C-reactive protein (CRP), uric acid (UA), lactate (LA) and physical characteristics were examined using Chi2maximum likelihood test and...

Author(s): T. Djarova, G. Watson, A. Basson, J. Grace, J. Cloete and A. Ramakoaba

Comparative effects of the leaves of Vernonia amygdalina and Telfairia occidentalis incorporated diets on the lipid profile of rats

January 2011

The study compared the effects of the leaves of Vernonia amygdalina (VA) and Telfairia occidentalis(TO) incorporated diet on the lipid profiles of rats. The rats were fed for 28 days on diets specially formulated to contain 5, 15 and 30% by weight respectively of the leaves of each plant while the control group was fed standard rat diet. The serum total cholesterol (TC), triacylglycerol (TG), high-density...

Author(s): C. E. Ugwu, J. E. Olajide, E. O. Alumana and L. U. S. Ezeanyika

Antioxidative activity and catechin content of four kinds of Uncaria gambir extracts from West Sumatra, Indonesia

January 2011

Gambir cubadak (GC), gambir udang (GU), gambir riau mancik (GRm) and gambir riau gadang (GRg) are popular cultivars of Uncaria gambir in Siguntur, West Sumatra, Indonesia. The aqueous extract of gambir has been used as traditional medicine to treat diarrhea, sore throat and some studies have attributed it to the presence of antioxidant properties. The purpose of this study was to characterize the...

Author(s): Tuty Anggraini,, Akihiro Tai, Tomoyuki Yoshino and Tomio Itani

Enrichment methods for revealing lower molecular weight biomarkers in human breast plasma proteome

January 2011

Breast cancer is the most common malignancy in women, comprising about 18% of all female cancers. The most important tools in screening and early detection are imaging techniques: mammography, ultrasonography and magnetic resonance imaging. However, up to 20% of new breast cancer incidents cannot be detected by these methods. Up to now, poor diagnosis of breast cancer is due to a lack of specific biomarkers....

Author(s): Bechr Hamrita, Hela ben Nasr#, Sami Remadi, Anouar Chaïeb and Karim Chahed,   #Bechr Hamrita and Hela ben Nasr contributed equally to this study and are co-first authors.

Correlation between moderate Plasmodium falciparum malarial parasitaemia and antioxidant vitamins in serum of infected children in South Eastern Nigeria

December 2010

The levels of antioxidant vitamins were estimated in Plasmodium falciparum malarial infected children aged 0 to 12 years. 113 children with P. falciparum infection were selected based on laboratory evidence and clinical symptoms. 87 apparently healthy children with no malarial parasitaemia were included as the control subjects. P. falciparum parasitaemia and serum levels of antioxidant...

Author(s): I. Onyesom, R. C. Ekeanyanwu and N. Achuka

The use of cholinergic biomarker, cholinesterase activity of blue mussel Mytilus edulis to detect the effects of organophosphorous pesticides

December 2010

The aim of the study was to investigate the effects of organophosphorous pesticide on thecholinesterase activity of different organs of Mytilus edulis. The mussels were exposed to serial dilutions of the pesticides (0, 50, 100, 200, 500, and 1000 µg/l) for 96 h. A significant inhibition of the cholinesterase activity from gill occurred at the lowest concentration, which indicated that gill was the...

Author(s): K. Yaqin and P. D. Hansen

Biochemical profile of sodium selenite on chemically induced hepatocarcinogenesis in male Sprague-Dawley rats

December 2010

Despite the success of experimental, clinical and epidemiological studies on selenium as an anti-cancer agent, basic studies on the effects of selenium are still scanty. This study was designed to investigate the biochemical effects of sodium selenite using preventive and therapeutic approaches on chemically induced hepatocarcinogenesis in rats. Rats were divided randomly into 6 groups: negative control, positive...

Author(s): Nasar Yousuf Alwahaibi, Siti Belkis Budin and Jamaludin Mohamed

Betaine reduces hyperhomocysteinemia and enhances 5-hydroxyindoleacetic acid in ethanol-induced hyperhomocysteinemia in rabbits

November 2010

Hyperhomocysteinemia is a hypothesis for the association of homocysteine with cerebrovascular diseases, neurodegenerative diseases and depression of mood. Thus, we examined whether oral betaine can act as a preventive agent in ethanol-induced hyperhomocysteinemia on the monoaminergic system. A total of 32 New Zealand White rabbits were divided into four groups (n=8); the Control group (C). The Ethanol group (E) was...

Author(s): Masoud Alirezaei, Mehdi Saeb, Katayoun Javidnia, Saeed Nazifi, Najmeh Khalighyan, and Saeedeh Saeb

Biochemical markers in semen and their correlation with fertility hormones and semen quality among Sudanese infertile patients

November 2010

Several biochemicals in semen are secreted by the accessory glands in the reproductive tract. This biochemical can be used as diagnostic predictors for the disorders in the male reproductive system. To assess the level of biochemical markers in semen, their relation to fertility hormones and spermogram among Sudanese infertile patients were studied. The biochemical markers studied were fructose, citric acid, zinc...

Author(s): Abdelmula M. Abdella, Al-Fadhil E. Omer and Badruldeen H. Al-Aabed

Effects of vitamin E and folic acid on some antioxidant enzymes activities of female Wistar rats administered combined oral contraceptives

October 2010

In this study, the effects of vitamin E and folic acid on the superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) production and glutathione-S-transferase (GST) activities in female wistar rats treated with combined oral contraceptives (COC) containing ethinyl estradiol in combination with levonorgestrel were determined. Twenty female rats were divided into four groups: group A (control) received distilled...

Author(s): Akinsanya, Mushafau  A., Adeniyi, Taiwo T., Ajayi, Gabriel O and Oyedele, Musbau A.

The prevalence of malaria infection in pregnant women living in a suburb of Lagos, Nigeria

October 2010

Malaria during pregnancy continues to be a major health problem in endemic countries with clinical consequences including death of both mother and child. In Nigeria, statistics shows that as many as 300,000 lives especially those of children and pregnant women are lost annually to malaria. This study was aimed at assessing the prevalence of malaria among pregnant women living in Ojo local council, a suburb of Lagos...

Author(s): Raimi, O. G., and Kanu, C. P. 

Alpha-amylase inhibitor of amadumbe (Colocasia esculenta): Isolation, purification and selectivity toward α- amylases from various sources

September 2010

Two proteins (A-1 and B-2) with a-amylase inhibitor activity  were extracted and partially purified from Colocasia esculenta tubers through 80% ammonium sulphate fractionation, ion-exchange chromatography on DEAE-Sephacel and gel-chromatography on Sephadex G-100. The molecular weight of A-1 and B-2 were estimated to be about 17000 and 19000 daltons, respectively. The inhibitors inactivated a-amylases of...

Author(s): R. McEwan, R. P. Madivha, T. Djarova, O. A. Oyedeji and A. R. Opoku

Influence of sub-chronic oral exposure to high monosodium glutamate on some serum markers of the renal functions in male Wistar rats

September 2010

Monosodium glutamate (MSG) is a flavor enhancing food additive that may be present in packaged food without appearing on the label. This could increase the possibility of its inadvertent consumption in high concentration. The study investigated the effects of MSG on some serum markers of renal functions in adult male Wistar rats by daily oral exposure to 3 ml kg-1 dose distilled water (DW) and 15 mg kg-1 MSG...

Author(s): Egbuonu Anthony C. Cemaluk, Ejikeme P. Madus and Obasi L. Nnamdi

Adsorption, metabolism and degradation of erythromycin in giant freshwater prawn and tilapia aquaculture in Mekong River Delta

September 2010

Adsorption, metabolism and degradation of erythromycin in freshwater prawn and tilapia aquaculture in Mekong River Delta were monitored and evaluated. They were fed practical diets medicated with erythromycin (50 and 100 mg. kg-1 body weight for 7 days). Erythromycin residues in their muscle were determined by the liquid chromatography - mass spectrometry/ mass spectrometry (LC-MS/MS) method. Our study provided...

Author(s): N. P. Minh, T. B. Lam and T. T. D. Trang

In vitro and in vivo evaluation of free radical scavenging potential of ethanolic extract of Podophyllum hexandrum

August 2010

The study was aimed at evaluating the antioxidant activity of ethanolic extract of rhizome of Podophyllum hexandrum under in vitro and in vivo situations. The extract was found to contain a large amount of polyphenols and also exhibit an immense reducing ability. At a concentration of 250 µg/ml, 48% of H2O2 radicals could be scavenged by the extract. The extract also inhibited...

Author(s): Showkat Ahmad Ganie, Mohmmad Afzal Zargar, Akbar Masood and Ehtishamul Haq

Expression of chitosanase gene from Aspergillus fumigatus JXSD-97 in Pichia pastoris

August 2010

In this study, the plasmid pPIC9K-CSN was transformed into Pichia pastorisstrain GS115 by electroporation and the high expression transformants with G418 resistance were obtained. The expression conditions for CSN in P. pastoris, such as the expression time, pH value and methanol concentration in the BMMY were optimized. The maximum activity of CSN is about 100 mg/L under optimized condition (96 h of 0.5%...

Author(s): Yang Li, Yang Ping and Wang Manying

Corncob is the optimum carbon resource for the growth of Tremella aurantialba

August 2010

The minimum medium without carbon source was used to investigate the effects of five kinds of hexoses, four kinds of pentose, four kinds of bi-saccharide and three kinds of polysaccharides on Tremella aurantialba growth. The result demonstrated that (1) The fittest carbon source of T. aurantialba was the five-carbon aldose in all the tested monosaccharides; (2) Polysaccharides linked with...

Author(s): Zhi-Cai Zhang, Mingxia Chen, Xin Li and Wangli Shen

Metabolic engineering of an ethanol-tolerant Escherichia coli MG1655 for enhanced ethanol production from xylose and glucose

August 2010

Efficient ethanol production will require a recombinant to able to ferment a variety of sugars (pentoses, and hexoses), less formation of by-products, as well as to tolerate high ethanol stress. In this study, a mutant (MGE) that can grow in 60 g ethanol/l was selected from Escherichia coli MG1655 by enrichment method with increasing concentrations of ethanol. The ethanol-tolerant mutant was used as the host...

Author(s): Ruiqiang Ma, Ying Zhang, Haozhou Hong, Wei Lu, Wei Zhang Min Lin and Ming Chen

Evaluation of nutritional and antinutritional characteristics of Obeche (Triplochition scleroxylon scleroxylon) and several Mulberry (Morus alba) leaves

July 2010

The biochemical composition of some selected varieties of White Mulberry and Obeche leaves were investigated to ascertain their nutritional and antinutritional values. Three varieties of White Mulberry leaves (S36, S54 and K2) were harvested from Ondo State sericulture centre while Obeche leaves were harvested from Aponmu Forest Reserve located in Ondo State, Southwestern Nigeria, for analysis. Proximate chemical...

Author(s): S. A. Adeduntan and A. S. Oyerinde

Phytochemical screening and histopathological effects of single acute dose administration of Artemisia annua L. on testes and ovaries of Wistar rats

July 2010

Artemisia annua L. is a medicinal plant whose derivatives are used in the treatment of malaria. Phytochemical screening and histopathological effects of single acute intraperitoneal dose of the alcoholic extract of A. annua, were investigated in Wistar rats in this study. Thirty five adult albino Wistar rats (109 - 307 g) of both sexes used for the study were randomly assigned on the basis of weight and...

Author(s): P. O. Ajah and M. U. Eteng

Standardized alcoholic extract of Phyllanthus fraternus exerts potential action against disturbed biochemical parameters in diabetic animals

July 2010

Alcoholic extract of Phyllanthus fraternus Webster whole plant (PFAE) prepared by successive solvent treatment was administered at a dose of 500 mg/kg body weight once in a day for 21 days to the alloxan induced diabetic albino rats. Certain biochemical parameters that is lipid profile (total cholesterol, high density lipoprotein, triacylglycerols), kidney functions (urea, creatinine) and liver functions...

Author(s): Munish Garg, Chanchal Garg, V. J. Dhar and A. N. Kalia

Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo

July 2010

The present study was carried out for identification of the phytochemicals present in the Vitex negundo leaves and also evaluate the total phenols, total flavonoids and antioxidant activity of the leaf extract. Total phenols was carried out by Folin Ciocalteu method and the phenolic content was 27.72 mg/100 of gallic acid equivalent (GE). Antioxidant activity was evaluated by DPPH method and the leaves...

Author(s): P. Praveen Kumar, S. Kumaravel and C. Lalitha

Effects of diet-induced hypercholesterolemia on the lipid profile and some enzyme activities in female Wistar rats

June 2010

The effects of a high dietary soybean oil and cholesterol on serum total cholesterol, low density lipoprotein (LDL-C), high density lipoprotein (HDL-C), and triglycerides were investigated. Total protein, albumin, glucose, gamma glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine amino transferase (ALT) activities were also investigated in weanling female Wistar rats for eight weeks. Two groups of...

Author(s): Gloria A. Otunola, Oyelola B. Oloyede, Adenike T. Oladiji and Anthony A. Afolayan

Malate dehydrogenase from blood stream Trypanosoma vivax exists in isoenzyme forms

June 2010

Malate dehydrogenase was isolated and partially purified from blood streamTrypanosoma vivax. The enzyme exists in isoenzyme forms (MDH1 and MDH2). The relative molecular weights of MDH1 and MDH2 determined by Sodium dodecyl sulphate polyacrylamide gel electrophoresis were 61 and 63 kdal. respectively. MDH2 lost activity immediately after purification. It has purification fold of 15 and percentage...

Author(s): A. U. Wurochekke, A. J. Nok, H. M. Inuwa and C. E. Gimba

Purification and characterization of the α-amylase isolated from Penicillium camemberti PL21

June 2010

The amylase family of enzymes is a great significance due to its wide area of potential application. α-amylase from Penicillium camemberti PL21 obtained from I. N. A. (The technological laboratory of Agriculture National Institute Paris- France) and using orange waste as substrates was produced under optimum conditions, after 168 h of incubation and subjected for purification and characterization. The...

Author(s): Tahar Nouadri, Zahia Meraihi, Djekrif-Dakhmouche Shahrazed and Bennamoun Leila

The distribution of cyanide detoxifying enzymes (rhodanese and 3- mercaptopyruvate sulphurtransferase) in different species of the family Cichlidae (Tilapia zillii, Sarotherodon galilaeus and Oreochromis niloticus)

June 2010

The cyanide metabolising enzymes (rhodanese and mercaptopyruvate sulphurtransferase) were estimated in different tissues of three species of tilapia (Tilapia zilli, Sarotherodon galileus,and Oreochromis niloticus) from two locations (Aiba and Osinmo reservoirs, both in the South-Western region of Nigeria). The enzyme activities were carried out by measuring the amount of thiocyanate produced by the two enzymes...

Author(s): Okonji Raphael Emuebie, Popoola Michael Olaoluwa, Kuku Adenike and Aladesanmi Omolara Titilayo

Characterization of antioxidant and antimicrobial compounds of cinnamon and ginger essential oils

June 2010

Essential oils obtained from the bark of Cinnamomum zeylanicum (cinnamon) and the rhizomes of Zingiber officinale (ginger) were characterized by analytical TLC and GC/MS, and their antimicrobial and antioxidant compounds were detected by TLC-bio-autography assays. Essential oil of cinnamon bark (CEO) was found to be a unique aromatic monoterpene-rich natural source, with trans-cinnamaldehyde...

Author(s): G. S. El-Baroty, H. H Abd El-Baky, R. S. Farag and M. A. Saleh

Production of yeast using acid-hydrolyzed cassava and poultry manure extract

May 2010

Cassava is made up of starch as its major nutritive reserve. Starch which is one of the most important products synthesized by plants is consumed as food and can be used in industrial processes. This investigation seeks to explore the availability of cassava as a source of glucose as well as poultry manure as a source of nitrogen in the production of yeast. Cassava flour was hydrolyzed with 0.5% (v/v) concentrated...

Author(s): L. I. Osumah and N. J. Tonukari

AQY1 gene from Debaryomyces hansenii

May 2010

Aquaporins are members of the major intrinsic protein superfamily of integral membrane proteins that enable the transport of water, glycerol and other solutes across membranes in diverse organisms. In yeasts, the proposed physiological roles of aquaporins are related to their contribution to freeze tolerance, osmoregulation and water transport. This article reports a contribution to the freeze tolerance in the...

Author(s): Juan Carlos González-Hernández

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