African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 418

AJBR Articles

Effects of Quantitative Trait Loci (QTLs) of BCL11A and HBS1L-MYB genes on clinical-biological variability of sickle cell disease in a Senegalese pediatric population

November 2022

High levels of hemoglobin F (HbF) could reduce the severity of sickle cell disease (SCD) by inhibiting hemoglobin S (HbS) polymerization. HbF expression is modulated by the Quantitative Trait Loci (QTLs) located on the HBS1L-MYB intergenic region (HMIP) (rs28384513) and intron 2 of the B-cell lymphoma/leukemia 11A (BCL11A) gene (rs4671393, rs1427407). To assess the impact of QTLs of HbF on clinical and biological...

Author(s): Tall Fatou G., Ndour El hadji M., Djité Moustapha, Barry Oumou K., Kandji Pape M., Gueye Papa M., Cissé Aynina and Sall Philoméne Lopez 

Physicochemical parameters of Blighia sapida (K.D. Koenig) oil extracted in Togo

November 2022

Oils extracted from Blighia sapida fruits are usually consumed without any information on its chemical composition. However the bad quality of oil can be a source of toxicity. Thus, the aim of this study is to investigate the physicochemical quality of the oil extracted from B. sapida from Togolese flora. To reach the goal of the study, mature and immature of B. sapida arils were used for oil extraction by the solvent...

Author(s): Aklesso Nabede, Haziz Sina, Tiatou Souho, Mamatchi Mélila, Farid T. Bade, Adolphe Adjanohoun, Batcha Ouadja, Lamine Baba-Moussa and Kou’santa Amouzou

Investigation into the intake of a popular polyherbal drug (Jalin Herbal Mannex Liquid) on selected biochemical indices of male wister rats

October 2022

Jalin Herbal Mannex Liquid (JHML) is a polyherbal formulation from honey, Panax ginseng, Liriosma ovate, and Lepidium mehenil, intended for enhanced sexual performance and improve sperm count in men. JHML was studied via its effect on body weight, biochemical indices and histopathology of the testes. Twenty healthy Wistar rats of 120 -150 g weight were allotted into groups A and B., of ten rats each, group A (control),...

Author(s): Kpomah, E. D. and Arhoghro, E. M.

Prevalence and associated risk factors of Vitamin D deficiency in children under five years of age, at the Diamniadio Children's Hospital in Senegal

October 2022

Vitamin D deficiency (VDD) is a public health problem which affects all human beings including dark-skinned subjects. In children, it can cause disabilities associated with skeletal abnormalities such as rickets or stunted growth. VDD is also associated with a significant risk of extra-skeletal, infectious, auto-immune, neoplastic, and cardiovascular diseases. The concentration of 25 (OH) D is currently considered as...

Author(s): Abou Ba, Najah Fatou Coly, Idrissa Basse, El Hadji Ibrahima Kane, Penda Awa Ka, Mamadou Soumboundou, , Souleymane Thiam, Abdourahmane Samba, Arame Ndiaye, Idrissa Yaya Soumah, Fatou Diedhiou, Fatou Cissé, Moustapha Djité, Néné Oumou Kesso Barry, Pape Matar Kandji, Papa Madiéye Gueye, Fatou Diallo Agne, Ndéye Ramatoulaye Diagne Gueye,

Antioxidants and antigenotoxic properties of Rhizopus oligosporus fermented cassava (Manihot esculenta Crantz)

September 2022

The antioxidant and antigenotoxic effects of solid state fermented cassava (Manihot esculenta Crantz) using Rhizopus oligosporus were investigated. Solid state fermentation was carried out for 72 h at room temperature under acidic and basic conditions. Total phenolic content was significantly (p<0.05) higher in fermented peeled and unpeeled cassava at pH 7 and 5 when compared with unfermented. Similarly, the total...

Author(s): Egoamaka O. Egbune, Theresa Ezedom, Akpovwehwee A. Anigboro, Eferhire Aganbi, Alex-Ifo Amata and Nyerhovwo J. Tonukari

Properties of antioxidants produced by Rhodobacter sphaeroides

May 2022

This study investigated the properties of antioxidants produced by Rhodobacter (R.) sphaeroides, a purple, non-sulfur photosynthetic bacterium. R. sphaeroides was fractionated into water-soluble and ethanol-soluble fractions, and the ethanol-soluble fraction showed stronger activity. Light irradiation significantly decreased the antioxidant activity of the ethanol fraction. The absorption spectra of the ethanol fraction...

Author(s): Deguchi Tomoaki, Ezaki Tomomi, Sato Miki and Kobayashi Masayuki 

Changes in lipid class and fatty acid composition during the development of African pear (Dacryodes edulis) fruit pulp

January 2022

The pulp of the African pear (D.edulis) fruits were investigated for its oil compositions, major lipid classes and constituents fatty acids from 4 weeks after anthesis (WAA) to fruit maturation.  The oil was extracted with n-hexane using soxhlet extractor and characterised by gas chromatography. Fatty acid profile showed a saturated acid content of between 19.5 and 36.5% and unsaturated fatty acid content of 63.5...

Author(s): ­Uhunmwangho E. S. and Omoregie E. S.

Amino acids and carbohydrates composition in breast milk of lactating mothers of different age group from Aleto Health Center, Rivers State

January 2022

Breast milk is the white liquid produced by women to breast-feed their babies. It has many benefits for infants, including reduced risk of gastroenteritis and respiratory infection. This study investigated the amino acids and carbohydrates composition in breast milk of lactating mothers. Eighteen (18) lactating mothers between the ages of 16-45 years participated in this study. Amino acid content was analysed with...

Author(s): Ndidi NWACHOKO, Udiomine B. AKURU, Fatima M. ODEJAYI and Jack Gbenenee TETAM

Medicinal effects of Saffron on obese animals by reducing Castelli’s risk index to protect from cardio-pathology and psychological distress

January 2022

A high-fat diet is a major factor to the global obesity and diabetes epidemic. The mechanism underlying chronic fat-rich food exposure may raise cholesterol and inflammatory mediator levels. One of the key pathological hallmarks of cardiovascular disorders is the development of inflammatory markers (CVDs). The pathological action may also inhibit the insulin secretion, which triggers diabetes-associated psychological...

Author(s): Saara Ahmad, Hamna Rafiq, Asra Khan, Prashant Tikmani, Saima Khaliq, Saad Bilal Ahmed and Muhammad Misbah ur Rehman

The anti-inflammatory activities of twelve Nigerian medicinal plants: Inhibition of NfkB, activation of Nrf2, and antioxidant content

January 2022

Chronic non-communicable diseases are responsible for a large majority of deaths worldwide. These include cardiovascular and respiratory diseases, diabetes and cancer. Inflammation has been reported to be involved in the initiation or progression of these conditions. Populations in developing or low to middle- income countries often rely on traditional medicine using locally available herbs and plants for their medical...

Author(s): Dorothy VanderJagt, Lucy Hunsaker, Sunday O. Otimenyin and Mary O. Uguru

Study of isoforms of nicotinamide adenine dinucleotide phosphate oxidase of the heart in a model of rats fed on several vegetable oils

October 2021

Palm oil has long been incriminated in obesity, and this obesity would be responsible for the development of cardiac fibrosis, several authors have evoked the role of free radicals in the pathophysiological mechanism of the fibrotic response linked to obesity; the aim of this study was therefore to evaluate the profile of NOX2 in the development of cardiac fibrosis in rats subjected to a diet rich in the fat of several...

Author(s): Koffi K. GervaIs, Jover Bernard, Badia Eric, Djohan Y Ferdinand, Reynaud Fabrice, Dere Luc, Niamkey Germaine, Chantal Gauze, Monde Absalome, Adeoti F Mansour, Camara-Cisse Massara, Cristol Jean Paul

Association of hyperuricemia and metabolic syndrome in type 2 diabetes mellitus patients in Dakar

October 2021

The association between hyperuricemia and metabolic syndrome (MS) has been reported in many studies. The authors performed this cross-sectional study to determine the association between hyperuricemia and the MS among diabetic patients in Dakar. Type 2 diabetic patients received as part of their follow-up at the Marc Sankalé Center of Abass Ndao Hospital in Dakar were enrolled. For each patient, blood samples and...

Author(s): Nènè Oumou Kesso Barry, Moustapha Djite, Pape Matar Kandji, El Hadji Malick Ndour, Michel Assane Ndour, Demba Diedhiou, Gueye-Tall Fatou, Alix Palanga Koboyo, Ndeye Marieme Thioune, Najah Fatou Coly, Dominique Doupa, Maimouna Ndour Mbaye, Aynina Cisse, Pape Amadou Diop, Philomène Lopez Sall, Papa Madieye Gueye,

Profile of CA 15-3 and CEA during breast cancer chemotherapy at Ouagadougou, Burkina Faso

October 2021

Biomarkers are considered as an innovative tool in the diagnosis and follow-up of breast cancer. The aim of the study was to assess the profile of circulating tumour markers CA 15-3 and CEA in patients under chemotherapy for breast cancer in Ouagadougou. This is a prospective cross-sectional study with a descriptive and analytical aims which was done from July to November 2020. Patients with histologically confirmed...

Author(s): Fabienne Marie SOUDRE, Alice KIBA, Arnaud KOURAOGO, Raoul KARFO, Aboubacar BAMBARA, Arsène KAGAMBEGA, Elie KABRE, and Jean SAKANDE,

Phytochemical and biological investigations of extracts from the roots of Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae), two plants used in traditional medicine

July 2021

Cocos nucifera L. (Arecaceae) and Carica papaya L. (Caricaceae) are two plants used to treat several human pathologies such as oxidative stress and inflammatory diseases. The aim of this study is to assess anti-inflammatory activity, antioxidant activity and acute oral toxicity, of aqueous and methanolic extracts from C. nucifera L. and C. papaya L. using appropriate experimental models. The acute oral toxicity test of...

Author(s): Wendkouni Leila M. Esther Belem-Kabré, Boukaré Kaboré, Adjaratou Compaoré-Coulibaly, Tata Kadiatou Traoré, Emmanuel A. M. Thiombiano, Mariam Nebié-Traoré, Moussa Compaoré, Félix B. Kini, Sylvin Ouédraogo, Martin Kiendrebeogo and Noufou Ouédraogo,

Evaluation of phytochemical constituents, proximate contents and glycemic index of bambara groundnut (Vigna subterranea L. Verdc) varieties grown in Northeastern Nigeria

July 2021

Bambara groundnut (Vigna subterranea L. Verdc) is widely used as a source of food and also has rich fibre and nutritional values. Hence, this research aimed at determining the phytochemical, proximate content as well as glycemic index and glycemic load of four varieties of Bambara groundnut found in Alkalere area of Bauchi State northeastern Nigeria for possible nutritional and medicinal utilization. Seeds of Bambara...

Author(s): Abdulrashid Mohammed and Hassan Daniel Mhya

Serum lipid profile based on the prandial state among adult subjects

July 2021

This study was aimed to access the variations in serum lipid parameters based on the prandial state of adult patients. Through a cross-sectional descriptive and analytical study conducted in Parakou (Benin), from February 1 to May 1, 2020, 100 adult subjects (average age 31.21±12.36 years) were selected with systematic census. After ethical advice, a first sample was taken on empty-bellied adult subjects. Then,...

Author(s): Moutawakilou Gomina, Corinne Tchegnonsi Tognon, Tarik Salifou, Gilbert Djidonou and Stanislas Zinsou  

Influence of the genetic polymorphism of haptoglobin in the occurrence of retinopathy and nephropathy in diabetics subjects

January 2021

The objective of this study was to determine the frequency of haptoglobin (Hp) genotypes and their association with nephropathy and retinopathy in diabetic subjects. This is a case-control study conducted in diabetic subjects developing nephropathy and/or retinopathy. Each patient was matched to a control of the same sex and the same age ± 2 years. Hp genotyping was performed by conventional PCR without enzymatic...

Author(s): SAGNE René Ngor, DJITE Moustapha, KANDJI Pape Matar, DIOP Jean Pascal Demba, BARRY Nene Oumou Kesso, THIOUNE Ndeye Marieme, NDOUR El Hadji Malick, GUEYE-TALL Fatou, NDIAYE-DIALLO Rokhaya, LOPEZ-SALL PhilomeÌ€ne, CISSE Aynina,  DIOP Papa Amadou,  NDOUR-MBAYE Maimouna and GUEYE Papa Madieye,

Sphenostylis stenocarpa (Hochst. Ex A. Rich) and fermented Parkia biglobosa ((Jacq, R.B.R.) supplementation prevents high fat diet induced derangements in kidney of albino rats

January 2021

Prevalence of obesity is on the increase globally with high fat diet (HFD) known to be the main contributing factor. This study was carried out to determine the actions of fermented Parkia biglobosa (Iru) and Sphenostyles stenocarpa (Otili) on the kidney of obese induced albino rats. The rats were grouped into a control group fed with normal rats chow and three different high fat diet groups (HFD1, HFD2, HFD3) mixed...

Author(s): Awoyinka O. A., Omodara T. R., Oladele F. C. Alese, M. O., Odesanmi E. O., Ajayi D. D., Adeleye G. S. and Omoleye O. F.

Evaluation of acute oral toxicity, hemato-biochemical activity and physiological responses of rabbits and rats administered Moringa oleifera leaf extract and meal

October 2020

The present study aims to evaluate acute oral toxicity of leaf extract of Moringa oleifera and determine the effect of the leaf’s meals of this plant on hematological and biochemical parameters of the rabbit. Ten rats weighing 150 ± 200 g were used for the oral acute toxicity study. Five rats received orally a single dose of 2000 mg/kg of weight of aqueous extract and 5 rats used as control....

Author(s): Basile Boni Saka Konmy, Pascal Abiodoun Olounladé, and Sanni-yo Doko-Allou,  

Correlation between glycated hemoglobin and venous blood sugar in diabetic patients monitored in Abidjan

October 2020

The aim of this study was to determine the correlation between glycated hemoglobin and blood sugar levels in diabetic subjects carried out in Abidjan. This cross-sectional study included 100 patients with diabetes monitored, for three months, for whom glycated blood glucose and hemoglobin were performed, this after informed consent of the patients. Pearson and Spearman correlation tests were used, at the 5% threshold....

Author(s): MONDE Aké Absalome, CAMARA-CISSE Massara, KOFFI Konan Gervais, DIALLO Issiagha, AKE AKE Alexandre, YAPO-AKE Bénédicte, ECRABEY Yann Christian, KOUAKOU Francisk, BENE YAO Roger Espérance and TIAHOU Georges

Insulin resistance and specific biomarkers in blood and urine of type 2 diabetic patients with or without nephropathy in Basrah, Iraq

October 2020

Diabetic nephropathy (DN) is a master cause of all surplus death-rate among type 2 diabetes mellitus (T2DM) patients with microalbuminuria. This study aimed to find effective biomarkers for early predicting of DN. Present study included 63 patients with T2DM (31 patients with DN, 32 patients without DN) and 33 healthy controls. These three groups were matched for their glucose, urea, creatinine, insulin, L-Carnitine...

Author(s): Adnan Jassim Mohammed Al-Fartosy, Nadhum Abdulnabi Awad and Sadoun Abbas Alsalimi,  

Sub-acute and protective effect of Cymbopogon citratus against carbon tetrachloride-induced liver damage

October 2020

The study evaluates the sub-acute toxicity and antioxidant potential of ethanolic leaf extract of Cymbopogon citratus against CCl4-induced toxicity in Sprague Dawley rats. The ethanolic leaf extract of C. citratus was prepared by solvent maceration method. The phytochemicals present in the extract were determined using standard methods. The potential sub-acute toxicities were evaluated using OECD procedure. The...

Author(s): Momoh Johnson Oshiobugie, Aderele Oluwaseun Raphael and Rasaq Adekunle Kareem

Antimalarial pyronaridine resistance may be associated with elevated MDR-1 gene expression profiles but not point mutation in Plasmodium berghei ANKA isolates

October 2020

The selection of resistance is inevitable whenever chemotherapy is necessary for pathogen control. Notably, Plasmodium falciparum has developed multifaceted means to overcome the toxicity of nearly all antimalarial medicines. To bypass this challenge, not only should novel drugs be developed, but the resistance mechanisms to new and existing drugs need should be fully explored. Pyronaridine is a companion drug in...

Author(s): Shadrack Kanyonji Kimani, and Jacob Manyiwa Shume,  

Study of the nutritional values and sanitary quality of soumbala collected from six regions of Burkina Faso

July 2020

Soumbala is regularly consumed as a condiment in most households in Burkina Faso. The purpose of this study was, therefore, to assess its nutritional and sanitary quality. To achieve this purpose, samples of sombala have been analysed for the determination of pH, water and ash contents, biochemical parameters, mineral elements, and aflatoxin contents. Results showed that Fada N’Gourma soumbala had the highest...

Author(s): Elie Kabré, Raoul Bazié, Moumouni Bandé, Thierry Sanou, Fulbert Nikiéma and Jean Sakandé,  

Antibacterial activity of endophytic fungi isolated from leaves of medicinal Plant Leucas martinicensis L. growing in a Kenyan tropical forest

July 2020

Infectious diseases are major threat to public health; a problem that has been exacerbated by emergence of multi-drug resistant (MDR) strains. Finding alternative antimicrobial compounds from natural sources such as fungal endophytes and medicinal plants is crucial for addressing antimicrobial resistance. Thus, in this study search for endophytes with antibacterial activities from leaves of medicinal plant Leucas...

Author(s): Ruth Moraa Obare, Stephen A. Indieka and Josphat Matasyoh  

Inhibitory potential and antidiabetic activity of leaf extracts of Vitex doniana

July 2020

Pancreatic alpha (α)-amylase and intestinal α-glucosidase involved in the enzymatic breakdown of dietary carbohydrates are implicated in postprandial hyperglycemia. The inhibition of these two enzymes has been exploited in the design of antidiabetic drugs. This study was therefore designed to investigate the ability of the leaf extracts of Vitex doniana to inhibit α-amylase and α-glucosidase as...

Author(s): Ani Onuabuchi Nnenna, Udedi Stanley Chidi, Anajekwu Benedette Azuka, Asogwa Kingsley Kelechi and Ekwealor Kenneth Udeh  

Evaluation of the effects of Azadirachta indica leaf on haematology, lipid profile, body weight and organ-system functions of streptozotocin-induced diabetic male rats

July 2020

This study was carried out to evaluate the effects of ethanol extract of Azadirachta indica leaf on haematological parameters, lipid profile, body weight, organ weight and histopathological functions of streptozotocin-induced diabetic rats. Diabetes was induced by a single intraperitoneal administration of streptozotocin (50 mg/kg bw.). The haematological parameters, lipid profile and histopathological investigations...

Author(s): Ezeigwe Obiajulu Christian, Okani Chukwudi Onyeaghana, Nnadi Naomi Ngozi, Obiukwu Onyinye Olivia, Ekwunoh Peter Okwukwe, Obayuwana Erhunmwense Ann, Okibedi Frances Uchenna and Obi Chioma Henrietta  

Evaluation of in vivo toxicity of rice husk used as fuel for cooking in households

May 2020

This study aimed to evaluate the toxicological effect of food cooked or water boiled with a fan-assisted top-lit-updraft rice husk fuelled gasifier stove named Paul Olivier 150 (PO150). Distilled water was boiled for 1 h using this stove in an opened pot and closed room. This water was then cooled to room temperature before being administered to rats with body weights ranging from 70 to 110 g. Two types of tests were...

Author(s): Mbassi Josiane Emilie Germaine, Sali Atanga Ndindeng, Achu Mercy Bih Loh, Dimo Théophile and Mbacham Fon Wilfred

Vitellaria paradoxa fruit pulp bioethanol production potential: A review

May 2020

In the last decade, bioethanol has become a powerful biofuel for the improvement of environmental pollution such as reduction in greenhouse gas levels. Yet, the source and type of substrate material plays a crucial role in the bioethanol production process due to the different compositional characteristics and availability of monomeric sugars. Different substrates of first, second and third generation fuel sources exist...

Author(s): Abdul-Mumeen Iddrisu, Zakpaa Hilary D., Felix Charles Mills-Robertson and Lowor Samuel T.  

Study of some biochemical parameters of the seeds of the fruit of the sweet Maniguette (Aframomum alboviolaceum (ridl.) k. Schum.) harvested in the Republic of the Congo

January 2020

Aframomum alboviolaceum is a plant that belongs to the family of Zingiberaceae, genus Aframomum and species of A. alboviolaceum. It is wide spread across tropical Africa and represented about 50 species. Proximate analysis is usually done to determine the values of macronutrients present in plant or food sample; it gives the composition of the biomass in terms of gross components. Proximate analysis includes...

Author(s): Jean Paul Latran Ossoko

Lambda-Cyhalothrin induced hepato-nephro toxicity potentials and post treatment recovery in Clarias garipinus

January 2020

This evaluates the 28-day toxicity and 7-day post treatment effect of LCT on the behaviour, liver and kidney of Clarias gariepinus. Prior to the experiment, fishes were acclimatized for two weeks. 120 fishes of standard length (SL) / weight (W) 10-12 cm, 8 - 17 g were used for median lethal concentration (LC50) test and 120 fishes of SL / W 16 - 40 cm, 200 - 250 g were used for the behavioural, hepato - nephrotoxicity...

Author(s): Samuel Uchenna Ezenwosu, Emmanuel Ikechukwu NnamonuÙ­, Gregory Ejikeme Odo, Ogonna Christiana Ani, Obiageli Constance Egilibe, Gladys Ukamaka Ogbodo and John F. Ebe

The effect of aqueous extract of zest of citrus sinensis (AEZCs) on cadmium chloride induced liver toxicity in wistar rats

January 2020

The potency of the peels of Citrus sinensis against cadmium induced liver damage has not been explored in our environment. 48 wistar rats were used for this study. The animals were randomly divided into eight (8) groups of five (5) rats each. Group A was the positive control and received 5 mg/kg body weight (bw) of cadmium chloride (CdCl2) intraperitoneally as a single dose. Groups B and C received the aqueous extract...

Author(s): Augustine Ndubuisi, Ani Celestine, Eze Wenceslaus, Ugwudike Patrick, Anyaeji Pamela, Ude Victor Chibueze, Agu Francis Uchenna, Nworgu Choice, Ikwuka David, Ugwuishi Emeka and Nwachukwu Daniel

Prevalence of dyslipidemia in a Burkinabe military population

January 2020

The objective of this study was to determine the prevalence of dyslipidemia in soldiers received at the laboratory of the Medical Center Camp General Aboubacar Sangoulé Lamizana (CMCGASL) for a medical visit. This is a prospective study of 224 military personnel assigned to peacekeeping missions outside Burkina Faso. The study was conducted over the period of September 2017 to November 2017. Included in the study...

Author(s): Raoul Karfo, Fabrice Mohamed Kangambega, Elie Kabre, Ouedraogo Paulette, Zakaria Nacro, Zakaria Sanogo, Adama Dao, Jean Sakandé and Lassane Sangaré

Increased levels of mannose-6-phosphate/insulin-like growth factor 2 receptor (M6P/IGF2-R) found in serum of patients with breast cancer

December 2019

The multifunctional transmembrane mannose-6-phosphate/insulin-like growth factor-2 receptor (M6P/IGF2-R) has been proposed as a putative prognostic marker in breast cancer. Loss of heterozygosity (LOH) with mutations in the remaining allele have been described at the gene level. We previously reported significant decrease of M6P/IGF2-R at the protein level in cancer cells than normal peritumoral ducts of breast tumors...

Author(s): Lemamy Guy Joseph, Ndeboko Benedicte, Omouessi Serge Thierry and Mouecoucou Justine

Proximate, mineral and phytochemical compositions of Vernonia amygdalina leaves grown under hot and humid tropical conditions

August 2019

This study was conducted to determine the proximate and phytochemical compositions of Vernonia amygdalina leaves, to ascertain its nutritional potentials. V. amygdalina plants were cultivated, and the leaves were harvested at maturity, cleaned and apportioned into two; one portion was oven dried whilst the other was air-dried- all to a moisture content of about 15%. The dried samples were pulverized and used for...

Author(s): Moses Teye, Victor Y. A. Barku and Faustina F. Yeboah  

The pro-oxidant effect of dextran sodium sulphate on oxidative stress biomarkers and antioxidant enzymes in Drososphila melanogaster

July 2019

This study sought to evaluate the behavioural and biochemical effects of Dextran Sodium Sulphate (DSS) on the oxidative stress biomarkers and antioxidant enzymes. Drosophila melanogaster (both sexes) of 3 days old were exposed to various DSS incorporated diets at concentrations, 0.5 - 3.0% for a period of 14 days (Survival rate). The second phase comprised of 3 groups; Group I- Control (normal diet), Group II-0.5% DSS,...

Author(s): Augustina O. Akinsanmi, Opeyemi Balogun, Johnson O. Titlayo, Ishaya Y. Longdet and Aguiyi C. John,

Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

July 2019

Six strains of Trichoderma species isolated from soil and air samples were screened for extracellular L-glutaminase production. The highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. A specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine,...

Author(s): Musa Olatunji Olarewaju and Humphrey Chukwuemeka Nzelibe  

Cadmium-induced toxicity and antioxidant enzyme responses in tissues and organs of African catfish (Clarias gariepinus)

June 2019

Juveniles of Clarias gariepinus were exposed to different concentrations of cadmium chloride for 96 h under laboratory conditions using static bioassays with continuous aeration to determine its mean lethal concentration (LC50), biochemical alterations, bioaccumulation and histological pattern in a sub-lethal toxicity test. The median lethal concentration (LC50) at the end of the acute toxicity was 120.2 mgL-1. Also the...

Author(s): Chisom P. OSISIOGU and Omolara T. ALADESANMI

Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

June 2019

The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the...

Author(s): Nihan Kübra Belligün and Burcu SaygıdeÄŸer Demir  

Phytochemical, acute and subacute toxicity studies of Annona senegalensis Pers. (Annonaceae) root wood extracts

May 2019

Considered safe because being from natural origin, plant products can exhibit toxic effects during their use. To ensure safe use of Annona senegalensis Pers. (Annonaceae), plant widely used for its therapeutic potential, the phytochemical and safety profiles of its root wood were investigated. Standard tube characterization tests were used to highlight phytochemical groups of root wood of the plant. Acute and subacute...

Author(s): Sylvain ILBOUDO, Hortense SOME, Geoffroy G. OUEDRAOGO, Félix B. KINI, Sylvin OUEDRAOGO and Innocent P. GUISSOU,

Immunomodulatory effects of cement in exposed workers

May 2019

This study was conducted to assess the immunomodulatory potentials [immunoglobulin E (IgE), interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α)] of cement dust in individuals occupationally exposed to cement. Potentials of cement particles to modulate immune responses have not been documented in occupationally exposed Nigerians. Twenty-nine male cement loaders who had direct exposure to cement...

Author(s): Moses O. Akiibinu, Taofeeq Oduola and Franklin Akinola  

Problems of interpretation of serum electrophoresis related to the manual phase during its realization with a semi-automatic automaton

April 2019

Manual steps including preparation of samples and gel, and launching of automatic sequences can cause many difficulties in the interpretation of electrophoresis. Three observations of electrophoretic profiles on Hydragel 15β1-β2 agarose gel media led us to look for interference in the manual step of the technique used. After reconsidering all the steps of the manipulation and carefully observing the profiles...

Author(s): Raoul Karfo,, Elie Kabré, Nadia Safir, Mounya Bouabdellah, Laila Benchekroun, Jean Sakandé, and Layachi Chabraoui,

Association of metabolic syndrome with the risk of developing liver disease in chronic hepatitis B patients

February 2019

Metabolic syndrome is a constellation of abnormal glucose and lipid metabolic parameter that increases ones risk of developing cardiovascular diseases. Metabolic profiles have been linked to progression of varying stages of liver disease in chronic hepatitis B infection. The main objective of this prospective cross sectional study was to establish a link between metabolic syndrome indicators and markers of progression...

Author(s): Dongsogo Julius and Larbie Christopher

Shea butter extraction technologies: Current status and future perspective

February 2019

Shea butter is a high–value shea nut fat used as an edible oil, antimicrobial and moisturiser in the food, pharmaceutical and cosmetic industries, respectively. The annual worldwide export of shea nut from Africa is 350,000 MT of kernels with a market value of approximately $120 million to producing countries. The multifunctional properties of the shea butter depend strictly on its compositional properties: the...

Author(s): Iddrisu Abdul-Mumeen, Didia Beauty and Abdulai Adam  

Studies on some physicochemical properties of Rhodanese synthesized by Bacillus cereus isolated from the effluents of iron and steel smelting industry

January 2019

The study purified and investigated the physicochemical properties of rhodanese (a cyanide detoxifying enzyme) synthesized by Bacillus cereus. This was with a view to producing an industrially important enzyme. The bacterial strain was identified as B. cereus by sequencing of its 16SrRNA gene. B. cereus rhodanese was purified with a fold of 3.53, yield of 36.80% and specific activity of 25.30 µmol/min/mg protein....

Author(s): Babamotemi Oluwasola Itakorode, Raphael Emuebie Okonji, Odunayo Adedeji, Nkem Torimiro, Chinwe Onwudiegwu and Ademakinwa Oluwaseyi  

Evaluation of the effect of vitamin C on caspase 9 and oxidative stress in rheumatoid arthritis patients

November 2018

Rheumatoid arthritis (RA) is a chronic and an autoimmune disease of the joints and is widely distributed worldwide. It is characterized by alterations of the antioxidant defense system and increased free radical formation and pro-inflammatory cytokine. The aim of the present study was to evaluate the effect of vitamin C supplementation on oxidative stress biomarkers and caspase 9 level in rheumatoid arthritis patients....

Author(s): Amira M. Elshamy, Nagah K. Gaafar, Nadia E. ElAshwah, Ayman A. Wagih and Abeer A. Shahba  

New insight on premature atherosclerosis in Egyptian children with β-thalassemia major

October 2018

Beta-thalassemia major (β-TM) in children is associated with an increase in the risk of premature cardiovascular complications caused by accelerated atherosclerosis which significantly contributes to morbidity and mortality. The molecular mechanisms underlying β-TM associated atherosclerosis and its relation to biochemical risk factors still remain obscure. We aimed to investigate the association between SIRT1...

Author(s): Hoda Ali Ibrahim, Soha Said Zakaria, Manal Mohamed Elbatch, Mohamed Ramadan Elshanshory and Samia Abd Elhamid Eldardiry  

Lipase in biodiesel production

September 2018

Although fossil fuels have been serving mankind for several centuries, their exploration and use bring about several environmental problems. Besides, fossil fuels are neither renewable nor sustainable. There is therefore a need to search for renewable alternatives which are sustainable and environmentally friendly. Biodiesel, chemically known as methyl ester, is a renewable and sustainable biofuel used to run diesel...

Author(s): Onyinyechukwu J. Chioke, Christiana N. Ogbonna, Chukwudi Onwusi and James C. Ogbonna  

The interplay between paraoxonase-1 and epigenetic changes in colorectal carcinoma

August 2018

Colorectal cancer (CRC) is the third leading cause of cancer-related death. Colorectal mucosa has high levels of oxidative stress (OS) landmarks. OS has deleterious effect on cell structures as lipids, proteins and DNA. Markers of protein oxidation are named as advanced oxidation protein products (AOPPs) which have been considered as novel disease biomarkers. OS may also induce DNA damage which leads to mutation of...

Author(s): El-Tokhy Amira Kamel, Zin El-deen Doaa Hussein, Foda Abd allah Mahmoud, Moussa Gamal Ibrahim and Abo El-Noeman Saad El-Din Abd el-fatah  

Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency (A-376/202) in homozygous sickle cell patients in Senegal and its clinical impact

July 2018

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common worldwide enzymopathy with approximately 400 million individuals affected. This inherited disease is sex-linked recessive inheritance. The high prevalence of certain variants of G6PD in different populations and ethnic groups increases the likelihood of finding associations with other pathologies. Sickle cell disease and thalassemia are the most...

Author(s): Dominique Doupa, Ousseynou Boye, Maguette Ndiaye, Mamadou Djité, Pape Matar Kandji, Sidy Mohamed Seck, Demba Makalou, Aminata Lam, Marie Pierre Diouf, Arame Ndiaye, Blaise Felix Faye, Souleymane Thiam, Abdourahmane Samba, Fatou Diallo, Papa Madieye Gueye, Alassane Diatta, Babacar Faye, Ibrahima Diagne and Saliou Diop  

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