African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 384

AJBR Articles

August 2019

Proximate, mineral and phytochemical compositions of Vernonia amygdalina leaves grown under hot and humid tropical conditions

This study was conducted to determine the proximate and phytochemical compositions of Vernonia amygdalina leaves, to ascertain its nutritional potentials. V. amygdalina plants were cultivated, and the leaves were harvested at maturity, cleaned and apportioned into two; one portion was oven dried whilst the other was air-dried- all to a moisture content of about 15%. The dried samples were pulverized and used for...

Author(s): Moses Teye, Victor Y. A. Barku and Faustina F. Yeboah  

July 2019

Statistical optimization of L–glutaminase production by Trichoderma species under solid state fermentation using African locust beans as substrate

Six strains of Trichoderma species isolated from soil and air samples were screened for extracellular L-glutaminase production. The highest enzyme producing isolate, Trichoderma longibrachiatum was subjected to screening and nutrients optimization experiments under solid state fermentation using a constant weight of African locust beans as substrate. A specific activity of 54.96 U/mg was achieved at 1% (w/v) glutamine,...

Author(s): Musa Olatunji Olarewaju and Humphrey Chukwuemeka Nzelibe  

July 2019

The pro-oxidant effect of dextran sodium sulphate on oxidative stress biomarkers and antioxidant enzymes in Drososphila melanogaster

This study sought to evaluate the behavioural and biochemical effects of Dextran Sodium Sulphate (DSS) on the oxidative stress biomarkers and antioxidant enzymes. Drosophila melanogaster (both sexes) of 3 days old were exposed to various DSS incorporated diets at concentrations, 0.5 - 3.0% for a period of 14 days (Survival rate). The second phase comprised of 3 groups; Group I- Control (normal diet), Group II-0.5% DSS,...

Author(s): Augustina O. Akinsanmi, Opeyemi Balogun, Johnson O. Titlayo, Ishaya Y. Longdet and Aguiyi C. John,

June 2019

Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the...

Author(s): Nihan Kübra Belligün and Burcu Saygıdeğer Demir  

June 2019

Cadmium-induced toxicity and antioxidant enzyme responses in tissues and organs of African catfish (Clarias gariepinus)

Juveniles of Clarias gariepinus were exposed to different concentrations of cadmium chloride for 96 h under laboratory conditions using static bioassays with continuous aeration to determine its mean lethal concentration (LC50), biochemical alterations, bioaccumulation and histological pattern in a sub-lethal toxicity test. The median lethal concentration (LC50) at the end of the acute toxicity was 120.2 mgL-1. Also the...

Author(s): Chisom P. OSISIOGU and Omolara T. ALADESANMI

May 2019

Immunomodulatory effects of cement in exposed workers

This study was conducted to assess the immunomodulatory potentials [immunoglobulin E (IgE), interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α)] of cement dust in individuals occupationally exposed to cement. Potentials of cement particles to modulate immune responses have not been documented in occupationally exposed Nigerians. Twenty-nine male cement loaders who had direct exposure to cement...

Author(s): Moses O. Akiibinu, Taofeeq Oduola and Franklin Akinola  

May 2019

Phytochemical, acute and subacute toxicity studies of Annona senegalensis Pers. (Annonaceae) root wood extracts

Considered safe because being from natural origin, plant products can exhibit toxic effects during their use. To ensure safe use of Annona senegalensis Pers. (Annonaceae), plant widely used for its therapeutic potential, the phytochemical and safety profiles of its root wood were investigated. Standard tube characterization tests were used to highlight phytochemical groups of root wood of the plant. Acute and subacute...

Author(s): Sylvain ILBOUDO, Hortense SOME, Geoffroy G. OUEDRAOGO, Félix B. KINI, Sylvin OUEDRAOGO and Innocent P. GUISSOU,

April 2019

Problems of interpretation of serum electrophoresis related to the manual phase during its realization with a semi-automatic automaton

Manual steps including preparation of samples and gel, and launching of automatic sequences can cause many difficulties in the interpretation of electrophoresis. Three observations of electrophoretic profiles on Hydragel 15β1-β2 agarose gel media led us to look for interference in the manual step of the technique used. After reconsidering all the steps of the manipulation and carefully observing the profiles...

Author(s): Raoul Karfo,, Elie Kabré, Nadia Safir, Mounya Bouabdellah, Laila Benchekroun, Jean Sakandé, and Layachi Chabraoui,

February 2019

Shea butter extraction technologies: Current status and future perspective

Shea butter is a high–value shea nut fat used as an edible oil, antimicrobial and moisturiser in the food, pharmaceutical and cosmetic industries, respectively. The annual worldwide export of shea nut from Africa is 350,000 MT of kernels with a market value of approximately $120 million to producing countries. The multifunctional properties of the shea butter depend strictly on its compositional properties: the...

Author(s): Iddrisu Abdul-Mumeen, Didia Beauty and Abdulai Adam  

February 2019

Association of metabolic syndrome with the risk of developing liver disease in chronic hepatitis B patients

Metabolic syndrome is a constellation of abnormal glucose and lipid metabolic parameter that increases ones risk of developing cardiovascular diseases. Metabolic profiles have been linked to progression of varying stages of liver disease in chronic hepatitis B infection. The main objective of this prospective cross sectional study was to establish a link between metabolic syndrome indicators and markers of progression...

Author(s): Dongsogo Julius and Larbie Christopher

January 2019

Studies on some physicochemical properties of Rhodanese synthesized by Bacillus cereus isolated from the effluents of iron and steel smelting industry

The study purified and investigated the physicochemical properties of rhodanese (a cyanide detoxifying enzyme) synthesized by Bacillus cereus. This was with a view to producing an industrially important enzyme. The bacterial strain was identified as B. cereus by sequencing of its 16SrRNA gene. B. cereus rhodanese was purified with a fold of 3.53, yield of 36.80% and specific activity of 25.30 µmol/min/mg protein....

Author(s): Babamotemi Oluwasola Itakorode, Raphael Emuebie Okonji, Odunayo Adedeji, Nkem Torimiro, Chinwe Onwudiegwu and Ademakinwa Oluwaseyi  

November 2018

Evaluation of the effect of vitamin C on caspase 9 and oxidative stress in rheumatoid arthritis patients

Rheumatoid arthritis (RA) is a chronic and an autoimmune disease of the joints and is widely distributed worldwide. It is characterized by alterations of the antioxidant defense system and increased free radical formation and pro-inflammatory cytokine. The aim of the present study was to evaluate the effect of vitamin C supplementation on oxidative stress biomarkers and caspase 9 level in rheumatoid arthritis patients....

Author(s): Amira M. Elshamy, Nagah K. Gaafar, Nadia E. ElAshwah, Ayman A. Wagih and Abeer A. Shahba  

October 2018

New insight on premature atherosclerosis in Egyptian children with β-thalassemia major

Beta-thalassemia major (β-TM) in children is associated with an increase in the risk of premature cardiovascular complications caused by accelerated atherosclerosis which significantly contributes to morbidity and mortality. The molecular mechanisms underlying β-TM associated atherosclerosis and its relation to biochemical risk factors still remain obscure. We aimed to investigate the association between SIRT1...

Author(s): Hoda Ali Ibrahim, Soha Said Zakaria, Manal Mohamed Elbatch, Mohamed Ramadan Elshanshory and Samia Abd Elhamid Eldardiry  

September 2018

Lipase in biodiesel production

Although fossil fuels have been serving mankind for several centuries, their exploration and use bring about several environmental problems. Besides, fossil fuels are neither renewable nor sustainable. There is therefore a need to search for renewable alternatives which are sustainable and environmentally friendly. Biodiesel, chemically known as methyl ester, is a renewable and sustainable biofuel used to run diesel...

Author(s): Onyinyechukwu J. Chioke, Christiana N. Ogbonna, Chukwudi Onwusi and James C. Ogbonna  

August 2018

The interplay between paraoxonase-1 and epigenetic changes in colorectal carcinoma

Colorectal cancer (CRC) is the third leading cause of cancer-related death. Colorectal mucosa has high levels of oxidative stress (OS) landmarks. OS has deleterious effect on cell structures as lipids, proteins and DNA. Markers of protein oxidation are named as advanced oxidation protein products (AOPPs) which have been considered as novel disease biomarkers. OS may also induce DNA damage which leads to mutation of...

Author(s): El-Tokhy Amira Kamel, Zin El-deen Doaa Hussein, Foda Abd allah Mahmoud, Moussa Gamal Ibrahim and Abo El-Noeman Saad El-Din Abd el-fatah  

July 2018

Prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency (A-376/202) in homozygous sickle cell patients in Senegal and its clinical impact

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common worldwide enzymopathy with approximately 400 million individuals affected. This inherited disease is sex-linked recessive inheritance. The high prevalence of certain variants of G6PD in different populations and ethnic groups increases the likelihood of finding associations with other pathologies. Sickle cell disease and thalassemia are the most...

Author(s): Dominique Doupa, Ousseynou Boye, Maguette Ndiaye, Mamadou Djité, Pape Matar Kandji, Sidy Mohamed Seck, Demba Makalou, Aminata Lam, Marie Pierre Diouf, Arame Ndiaye, Blaise Felix Faye, Souleymane Thiam, Abdourahmane Samba, Fatou Diallo, Papa Madieye Gueye, Alassane Diatta, Babacar Faye, Ibrahima Diagne and Saliou Diop  

June 2018

Bacteriostatic and Bactericidal effects of ethyl acetate root bark extract of Terminalia avicennioides on Methicillin-resistant Staphylococcus aureus

Root bark extract of Terminalia avicennioides was obtained by cool maceration with 750 ml n-hexane, chloroform, ethyl acetate and methanol, independently for 48 h using soxhlet extractor. ATCC 33591 standard strains of methicillin-resistant Staphylococcus aureus (MRSA) was used. Strain resistance to methicillin/oxacillin antibiotic was verified by a retest of its sensitivity to oxacillin antibiotic. The antimicrobial...

Author(s): Adim Ekene Ugochukwu, Dingwoke Emeka John, Adamude Fatima Amin, Edenta Chidi, Nwobodo Ndubuisi Nwobodo, Offiah Raymond Ogbonna, Onyeka Ifeanyi Peter and Ezeaku Ikenna Nnamdi

May 2018

Biochemical characterisation of soybean germplasms with respect to bioavailable iron and zinc, vitamin A and crude protein

Soybean germplasms were assessed for bioavailable zinc (Zn) and iron (Fe), vitamin A and crude protein using standard methods. Thirty soybean germplasms were investigated. Results were analysed using analysis of variance (ANOVA) and showed statistically significant differences in their Zn, Fe and vitamin A concentrations, as well as in crude protein content (P < 0.05). The Zn content ranged from 5.74 ± 0.04 to...

Author(s): Titilayo Akande, Francis N. Onyezili and Joseph Ikwebe  

March 2018

Effects of garden egg, carrot and oat-supplements on biochemical parameters in cadmium exposed rats

Cadmium (Cd) toxicity is influenced by dietary components, such as fiber and minerals. Garden egg, carrot and oat are rich sources of fiber. Thus, this study examines the effect of Solanum melongena (garden egg), Daucus carota (carrot) and oat-supplements on selected biochemical parameters in the plasma and tissues of cadmium-exposed rats. Twenty-five healthy male Wistar rats (140±50 g) were distributed into five...

Author(s): Orororo O. Clement, Samuel O. Asagba, Oghri Emus and Egbune E. Oliseneku

March 2018

Effect of organic acids on the alteration of black rice anthocyanins by Enterobacter aerogenes NBRC 13534

In a previous study, it was reported that Enterobacter aerogenes and four organic acids are involved in the alteration of black rice anthocyanins. To investigate the factors involved in altering the coloration of black rice anthocyanins, organic acids (caffeic acid, ferulic acid, malonic acid, and p-hydroxybenzoic acid) were individually added to potato dextrose agar medium containing black rice anthocyanins and...

Author(s): Keiko YAMAMOTO, Noriaki SAIGUSA and Yuji TERAMOTO  

February 2018

Evaluation of nutritional, anti-nutritional and some biochemical studies on Pleurotus squarrosulus (Mont.) singer using rats

Evaluation of nutritional, anti-nutritional and some biochemical studies on Pleurotus squarrosulus (Mont.) Singer using rats was undertaken with standard methods. Results of nutritional contents such as proximate composition revealed moisture (11.67%), crude protein (21.31%) and total carbohydrate (51.58%). Vitamin composition showed the presence of vitamins A (0.17 mg/kg), B2 (0.62 mg/kg), B3 (0.45 mg/kg), B6 (0.84...

Author(s): Duru Majesty, Nwadike Constance, Ezekwe Ahamefula, Nwaogwugwu Caleb, Eboagwu Ijeoma, Odika Prince, Njoku Samuel, and Chukwudoruo Chieme  

January 2018

Preventive action of zinc against heavy metals toxicity in honeybee

A detrimental consequence of industrial growth has been the steady increase in heavy metal pollution which has major, negative impacts on living organisms. Heavy metals still pose a significant health threat, despite the implementation of many strategies to reduce pollution. The aim of this study was to investigate the possible effects of zinc on the accumulation of some heavy metals in honeybees. Groups totalling 15...

Author(s): Cevat Nisbet, Ahmet Güler , Neslihan Ormancı and Sena Cenesiz

December 2017

Reactive oxygen species (ROS) generation, impacts on tissue oxidation and dietary management of non-communicable diseases: A review

Generation of reactive oxygen species (ROS) in biological systems has been reported to be a significant cause of inflammatory and metabolic diseases. More recently, ROS and in a particular ozone has also been implicated in the conversion of cholesterol to atherogenic compounds, secosterol A, and upon aldolization to secosterol-B. Secosterol-A is uniquely produced by cholesterol ozonolysis, while secosterol-B can also be...

Author(s): Wanjala George Wafula, Onyango Arnold, Onyango Calvin and Makayoto Moses

November 2017

Therapeutic effect of an alkaloid-rich fraction of Abrus precatorius seed methanol extract on paracetamol-induced liver damage in rats

The therapeutic effect of an alkaloid-rich fraction of the chloroform-methanol extract of Abrus precatorius seeds on paracetamol-induced hepatotoxicity in rats was investigated in this study. The extract was fractionated in a 17.5 × 2.5 cm Sephadex G15 swollen, packed and eluted with water. The fractions were spotted on F2.54 pre-coated thin layer chromatography (TLC) plates and sprayed with Drangendorff’s...

Author(s): Ukegbu Chimere Young, Parker Elijah Joshua, Okwesili Fred Nwodo C., and Iroha Okechukwu  

October 2017

Chemical composition, antibiotic promotion and in vivo toxicity of Piper nigrum and Syzygium aromaticum essential oil

The antimicrobial activity of spices of human daily diet has been widely demonstrated. Yet, their capacity of promoting antibiotic activity has not been explored adequately. In this work, the chemical composition of essential oils of Piper nigrum and Syzygium aromaticum from Chad were determined and their antimicrobial effect in combination with different antibiotics towards Staphylococcus aureus, Salmonella enteritidis...

Author(s): M. Ismail, G. A. Kemegne, F. N. Njayou, V. Penlap, W. F. Mbacham, and S. L. S. Kamdem,

August 2017

Haptoglobin polymorphism and cardiovascular risk factors in followed epileptic patients at Fann National University Hospital

The aim of our research was to evaluate the cardiovascular risk factors on epileptic patients treated at the Fann University Hospital and to study the influence of haptoglobin (Hp) polymorphism on disease progression. In order to do that, eighty-six (86) patients followed in neurology for at least 2 years were recruited. Each patient was matched to a control according to age and sex. Hp phenotyping was performed by...

Author(s): Papa Madièye GUEYE, Moustapha DJITE, El Hadji Malick NDOUR, Fatou GUEYE-TALL, Néné Oumou Kesso BARRY, Aïssatou SARR, Rokhaya NDIAYE DIALLO, Philomène LOPEZ SALL, Aynina CISSE and Pape Amadou DIOP

July 2017

Optimization of amylase production by Aspergillus niger cultivated on yam peels in solid state fermentation using response surface methodology

The study involved the production of amylase from Aspergillus niger grown on yam peels in solid state fermentation. The process parameters: temperature, pH (initial) and incubation time were optimized for maximum amylase production using central composite design (CCD) of response surface methodology (RSM). Temperature was the most significant (p<0.05) parameter and the maximum interaction occurred between temperature...

Author(s): Samuel Kwatia Victoria Pearl Dzogbefia and Isaac William Ofosu

June 2017

Effects of ethanol and aqueous leaf extracts of Pterocarpus mildbraedii on hematology parameters and cholesterol in albino rats

One of the commonly consumed leafy vegetables in Nigeria is Pterocarpus mildbraedii Harms (Leguminosae) leaf. It has been found that, ethanol and aqueous extracts of the plant leaves exert anti-diabetic effect on rats. This study investigated the effects of ethanol and aqueous leaf extracts of this plant on some hematological parameters and serum cholesterol level in Wistar albino rats. Intraperitoneal administration of...

Author(s): Adegbite A. Victor and Ezekwesili C. Nonyelum

May 2017

Antimibacterial activity of saponins from Sanseviera trifasciata prain cv. golden hahnii roots on Escherichia coli and Staphylococcus aureus

Sansevieria-trifasciata-is commonly known as both in and out door house plant. It is also used in traditional medicine for treatment of influenza, cough and respiratory inflammation. The roots and leaves have secondary metabolites such as saponins that exbihit remedy for cough, snake bite, sprain, bruise, boil, abscess, respiratory inflammation and hair tonic.  The plant sample was collected and dried. The...

Author(s): Whika Febria Dewatisari, Subandi and Desmawati

April 2017

Effects of date fruit extract on paracetamol induced nephrotoxicity in wistar rats

This study was aimed to investigate the protective, ameliorative and preventive effects of aqueous Date fruit extract on paracetamol induced nephrotoxicity in wistar rats. A total of 30 male albino wistar rats were used for this study. Rats were randomly divided into five groups containing 6 animals each. Treatments were given daily for fourteen days. The animals in the control group (Group I) did not receive any...

Author(s): Tijani Idris Ahmad Oseni, Nurah Tijani Oseni, Hauwa Tijani Oseni and Pauline Etuaje Eromon

March 2017

GC-MS/FT-IR screening of Xylopia aethiopica (Dunal) A. Rich Fruit

The present study was aimed at identifying the functional groups and phyto-constituents present in Xylopia aethiopica (Dunal) A Rich fruit using Fourier Transform Infrared Spectrometry (FTIR) and Gas chromatography-mass spectroscopy (GC-MS), spectroscopy. FTIR method was performed using Perkin Elmer Spectrophotometer and the characteristic peaks were detected. The phytochemical constituents were screened by GC-MS method...

Author(s): Okereke S. C., Arunsi U. O. and Nosiri C. I.

February 2017

Antioxidative activity of Tamarindus indica L. extract and chemical fractions

Tamarindus indica L is a tropical plant that is used around the world mainly for medicinal purposes. Yet few scientific studies of its biological potential have been undertaken. The aim of this study was to assess the antioxidative potential of T. indica L leaves. The plant material was harvested and then dried in the absence of direct sunlight. The extraction was done by alcoholic decoction, and the fractions were...

Author(s): Amadou Ibrahima Mbaye, Papa Madieye Gueye, Alioune Dior Fall, Modou Oumy Kane, Kady Diatta Badji, Abdou Sarr, Daouda Diattara and Emmanuel Bassene

January 2017

Effects of Xylopia aethiopica fruit extract on isolated rabbit ileum

In this study, pharmacological reactivity of rabbit ileum to crude extract of fruits of Xylopia aethiopica in the presence of other agonist and antagonist agents was evaluated. The contractility of the isolated rabbit ileum was recorded using kymograph. The effects of the crude extract of X. aethiopica on rabbit ileum were independently determined using a dose range of 10-8 to 10-4 mg/ml. However, to determine the...

Author(s): Okwari O.O, Obembe A.O, Jeje S.O, Honesty E.E and Osim E.E

November 2016

Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica

In this study, the extraction conditions of polysaccharides of Artemisia mongolia (PAM) were optimized and their molecular weight, weight distribution, composition and antioxidant activities of the polysaccharides investigated. The degree of polymerization of fructan-oligosaccharides 2-8 was separated by thin layer chromatography (TLC) with good results. Polysaccharides were extracted from the leaves of A. mongolia and...

Author(s): Yahaya Kudush Kawa, Quinck Shang, Shangyi Tian, John Paul Kaisam and Victoria Kabia

November 2016

Effect of pre-harvest chitosan foliar application on growth, yield and chemical composition of Washington navel orange trees grown in two different regions

The present study was carried out during 2012 and 2013 seasons to study the effect of pre-harvest foliar application of chitosan (a natural beta-1-4-linked glucosamine polymer) at two concentrations 250 and 500 ppm on vegetative tree growth, fruit yield and quality as well as leaves chemical composition of Washington navel orange trees grown under two  locations. As for growth parameters (shoot length, leaves...

Author(s): Ahmed Hussien Hanafy Ahmed, Mohamed Ramadan Aboul-Ella Nesiem, Hesham Ali Allam and Amira Fahmy El-Wakil

October 2016

Protease inhibition in cowpea pod-sucking bug species (Hemiptera: Heteroptera) using cereal and legume grain extracts

Selected grain extracts were assayed against digestive proteases of Anoplocnemis curvipes, Clavigralla tomentosicollis and Mirperus jaculus with a view to identifying suitable sources of protease inhibitors against the cowpea pests. Laboratory bioassays showed that grains of a local soybean variety Kìshí and a cowpea landrace NG/SA/07/0098 contain potent inhibitors although the former produced a...

Author(s): Abiola Elfrida Taiwo, Olalekan Joseph Soyelu and Raphael Emuebie Okonji

September 2016

Protective effects of extract and fraction of root- bark of Garcinia kola (Heckel) on the renal biochemical parameters of gentamicin-induced nephrotoxic rats

The study evaluated the nephroprotective potentials of methanolic extract (ME) and dichloromethane fraction (DCMF) of root-bark of Garcinia kola on gentamicin-induced nephrotoxicity in rats with a view to utilize the extracts of the root of G. Kola in the amelioration of antibiotic-induced kidney damage. The study involved preparation of the extract, fractionation, phytochemical screening and evaluation of phenolics and...

Author(s): Komolafe, Ige Joseph, Akinlalu, Akintunde Oluseto, Ogunsusi, Mayowa and Oyedapo, Oluboade Oluokun

May 2016

Oil palm fruit calyx as a resource for phospholipids extraction

Oil palm calyx is a solid waste in the oil palm industry which is yet to find economic usage. This study aimed to determine the feasibility of recovering phospholipids from the oil palm calyx. Preliminary functional property of calyx phospholipids was determined using emulsion stability. Results indicated that oil palm calyx had 14% total lipids when extracted with methanol and 62.3% of lipids were acetone insoluble...

Author(s): Gold I. L., Oseni N. T. and Imoisi O. B.

March 2016

Antihyperlipidaemic and anti-atherosclerotic activities of aqueous leaf extract of L. africana in streptozotocin induced diabetic rats

Hyperlipidaemia is a usual and life threatening complication of diabetes mellitus. Consequently, a good anti-diabetic agent should reduce the progression of this complication. In view of this, the anti-hyperlipidaemic and anti-atherogenic activities of aqueous extract of Lasianthera africana was studied in streptozotocin induced diabetic rats. Extract treatment was administered at a dose of 1ml of 180 mg/ml of extract...

Author(s): Uwakwe Augustine  A., Miikue-Yobe Togenu F. B., Akaninwor Joyce O. and Felix-Samuel Barisi

February 2016

Bio-enrichment of phenolics and free radicals scavenging activity of wheat (WH-711) fractions by solid state fermentation with Aspergillus oryzae

This study was conducted to determine the total phenol content (TPC) and free radicals scavenging activity of methanolic and ethanolic extracts of wheat (WH-711) fractions (grain, bran and flour) fermented with Aspergillus oryzae (MTCC 3107). Maximum TPC (1469.35 ± 4.5, 1426.44 ± 5.5 and 1268.91 ± 5.3) was obtained in methanolic extracts as compared to ethanolic extracts (1368.65 ± 3.5,...

Author(s): Joginder Singh Duhan, Kamal Mehta, Pardeep Kumar Sadh, Pooja Saharan and Surekha

January 2016

Modulation in membrane lipids of Escherichia coli and Staphylococcus aureus in response to varying NaCl concentrations

The effect of varying NaCl concentrations on membrane lipids of Escherichia coli and Staphylococcus aureus cultivated at 37°C was studied. Lipid profiles of the membrane extracts were resolved by thin layer chromatography (TLC) using polar and neutral lipids solvent systems, with their fatty acid constituents determined via gas chromatography-mass spectrometry (GC-MS). Polar lipids identified were cardiolipin,...

Author(s): Omotoyinbo Oluwasegun Victor, Sanni David Morakinyo and Olawoye Theophilus

January 2016

Effect of kidney bean consumption on some lipid and haematological parameters of albino rats

This study investigated changes in cholesterol levels and haematological parameters of rats subjected to diets supplemented with kidney bean seeds. The seeds were processed in three parts; the first part was milled raw; the second part was boiled, dried and milled; third part was germinated, dried and milled. The kidney bean seed flour was incorporated into the diet of different groups of rats at 10% inclusion. Thirty...

Author(s): Olanipekun, O. T., , Balogun, E. A., Akinloye, O. A., and Omotainse, S. O.

December 2015

Production and antioxidant activity of alcoholic beverages made from various colored rice and wild rice

In order to develop a new type of alcoholic beverage with antioxidant activity, the authors carried out this research. In this study, alcoholic beverages were made from unpolished black rice, unpolished red rice, unpolished green rice, polished white rice and unpolished wild rice using conventional cooked fermentation and economical uncooked fermentation system. The resulting alcoholic beverages made from cooked and...

Author(s): Ryoichi TAKESHITA, Noriaki SAIGUSA and Yuji TERAMOTO

November 2015

Extraction, fractionation and assessment of antioxidant activities of active components of Aframomum sceptrum seeds

A simple procedure of extraction, fractionation and assessment of organoleptic and antioxidant properties of Aframomum sceptrum seeds was carried out, and the results were compared with a currently used synthetic antioxidant, butylated hydroxy toluene (BHT). Extraction was carried out using Soxhlet extractor and fractionation of the ethanolic extract was accomplished using vacuum liquid chromatography (VLC). Various...

Author(s): Nwankwo Patience Okwuchi

November 2015

Nitrate contents in some vegetable leaves in Sokoto Metropolis, Nigeria

Popular Nigerian vegetables namely, cabbage, spinach, bitter leaf, water leaf, ewedu, roselle and lettuce obtained from Sokoto metropolis, Nigeria were analysed for nitrate contents by ultra-violet (UV)-spectrophotometric method. The fresh leaves of the samples were chopped and ground using mortar and pestle for the analysis. Absorbance of each sample was obtained in three replicate and the calibration graph of...

Author(s): Abdullahi, A. S., Usman, J., Muazu, S., Abba, Y. and Ibrahim, M. K.

October 2015

Production of bacterial amylases and cellulases using sweet potato (Ipomoea batatas. (L.) Lam.) peels

Peels of sweet potato (Ipomoea batatas) were buried in the soil for 14 days and the isolates associated with the degradation of the peels were obtained using standard microbiological procedures. The bacterial isolates obtained were screened for amylolytic and cellulolytic activities under different pH and temperatures as parameters and optimized for enzyme production. Sixteen (16) bacterial isolates were obtained and...

Author(s): Olanbiwoninu Afolake Atinuke and Fasiku Samuel 

October 2015

Comparative effects of peel extract from Nigerian grown citrus on body weight, liver weight and serum lipids in rats fed a high-fat diet

The effects of ethanolic extracts of five different citrus peels on mean body and liver weight and serum lipid content were investigated in albino rats. Six groups (n=8 each) were fed with a high fat diet for seven days ad libitum before oral daily administration of the peel extracts of orange (OR), lemon (LE), lime (LI), tangerine (TA), grapefruit (GR) and synergistic combination of equal ratios (w/w), that is, (SY),...

Author(s): Josephine Ozioma Ezekwesili-Ofili and Ngozi Christine Gwacham

September 2015

Association of single nucleotide polymorphism of Hsp90ab1 gene with thermotolerance and milk yield in Sahiwal cows

Heat shock proteins play a critical role in the development of thermotolerance and protection from cellular damage associated with heat stresses. The study was undertaken to investigate the association of single nucleotide polymorphisms (SNPs) of Hsp90ab1 gene with thermo-physiological parameters viz, respiration rate (RR), rectal temperature (RT), heat tolerance coefficient (HTC) and total milk yield in Sahiwal cows....

Author(s): Lalrengpuii Sailo, I. D. Gupta, Archana Verma, Ramendra Das and M. V. Chaudhari

August 2015

Effect of fermentation methods on the mineral, amino and fatty acids composition of Cyperus esculentus

Tiger nut (Cyperus esculentus) was subjected to different fermentation methods such as traditional, back slope and control. The raw and fermented samples were analyzed for mineral, amino and fatty acids. The results of mineral analysis revealed potassium and sodium as the most abundant mineral element with their value ranging from 546 to 91.6 mg/100 g and 64.00 to 3383.33 mg/100 g, respectively while copper was found in...

Author(s): Agbaje, R. B., Oyetayo, V. O. and Ojokoh, A. O.

August 2015

The effect of aqueous leaf extract of fluted pumpkin on some hematological parameters and liver enzymes in 2,4-dinitrophenylhydrazine- induced anemic rats

Anemia constitutes a serious health problem in many tropical countries including Nigeria because of the prevalence of malaria and other parasitic infections which possibly leads to decrease of hemoglobin. Fluted pumpkin has been reported to be very good in building the constituents of the blood and also replacing them. This study was designed to investigate the effects of the aqueous leaf extract of fluted pumpkin on...

Author(s): Toma, I., Victory, N. C. and Kabir, Y.

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