African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 407

AJBR Articles

Nicotinamide: A cytoprotectant against streptozotocin-induced diabetic damage in Wister rat brains

September 2008

  Nicotinamide is being used in experimental and clinical trials examining the prevention of type-1 diabetes mellitus. However, the precise mechanisms underlying the antidiabetic and neuroprotective effects of nicotinamide require further analysis. Our goals are to evaluate the protective effect and the cellular and molecular mechanisms of nicotinamide against brain damage induced by type-1 diabetes in...

Author(s): Safinaz S. Ibrahim and Sherine M. Rizk

A 22-bp sequence of the core promoter from the Indica rice sucrose-phosphate synthase gene (sps1) is sufficient to confer basal transcription activity

August 2008

  The 5’ region of the sucrose-phosphate synthase gene (sps1) of the Indica riceconsists of an atypical promoter which lacks TATA box but has a putative initiator sequence overriding a second transcription initiation site. Analysis of the transient expression of truncated versions of the sps1 promoter (from -148 to +21) fused to the uidA reporter gene was performed. The...

Author(s): Miguel Martínez-Trujillo, Gamaliel Valdivia-Rojas, Gloria Solís Guzmán, José Luis Cabrera-Ponce

Nicotinamide: A cytoprotectant against streptozotocin-induced diabetic damage in Wister rat brains

August 2008

  Nicotinamide is being used in experimental and clinical trials examining the prevention of type-1 diabetes mellitus. However, the precise mechanisms underlying the antidiabetic and neuroprotective effects of nicotinamide require further analysis. Our goals are to evaluate the protective effect and the cellular and molecular mechanisms of nicotinamide against brain damage induced by type-1 diabetes in...

Author(s): Safinaz S. Ibrahim and Sherine M. Rizk

Comparative study on the determination of assay for laccase of Trametes sp.

August 2008

The aim of this work was to determine the sensitivity among compounds in common use for detecting laccase activity. In this study, three assay procedures to measure laccase activity of Trametes sp. were performed in Kirk’s basal salts mediums of three dyes. In the assay methods, three substrates were employed, which were 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), ο-dianisidine...

Author(s): Airong Li, Yue Zhu, Liang Xu, Wenqing Zhu and Xingjun Tian

Nitric oxide scavenging ability of ethyl acetate fraction of methanolic leaf extracts of Chromolaena odorata (Linn.)

July 2008

  The nitric oxide (NO.) scavenging activities of Chromolaena odorata (Linn) King and Robinson, was investigated and compared with standard Plant Phenolic antioxidant Quercetin. Disodium pentacyanonitrosyl ferrate (2- ) dihydrate (sodium nitroprusside) was used as a nitric oxide donor. On disintegration at a physiological pH (7.2), 5 mmol/l of sodium nitroprusside generated a time dependent nitric...

Author(s): Alisi, C. S and Onyeze, G. O. C

Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant irrigated with sea water

July 2008

  Effect of irrigation bread wheat plants (Triticum aestivum L., cv. Giza 94) with sea water (10 and 20% v/v), spraying with microalgae extracts obtained from Chlorella ellipoida and Spirulina maxima (5 gL-1 dry weight in 0.1% Tween solution) cultivated under normal and stress conditions were studied. Some plant bioregulators (BRGs, ascorbic acid and benzyl adenine, at 200...

Author(s): Hanaa H. Abd El-Baky, Hussein M. M.  and Game S. El-Baroty

Effect of administration of aqueous and ethanolic extracts of Enantia chlorantha stem bark on brewer’s yeast-induced pyresis in rats

July 2008

  The phytochemical screening of aqueous and ethanolic extracts of the stem bark of Enantia chlorantha Oliv as well as the antipyretic effect of the solvent extracts at 25, 50, 100 and 200 mg/kg body weight was investigated in albino rats. Phytochemical analysis of the aqueous and ethanolic extracts of E. chloranthastem bark revealed the presence of phenolics, flavonoids, alkaloids, glycosides...

Author(s): Adesokan, A. A., Yakubu, M. T., Owoyele, B. V., Akanji, M. A., Soladoye, A. O. and Lawal, O. K.

Acid phosphatase from snail heamolymph: a cheap and convenient source of enzyme for kinetic parameters determination

June 2008

  The study of enzymes occupies a very important position in biological, medical and industrial research. Also, the satisfactory performance of an enzyme assay provides a good introduction to biochemical techniques. This experiment presents a good introductory project to students for the estimation of the kinetic parameters of an enzyme. The spectrophotometric/colorimetric assay of acid phosphatase is very...

Author(s): Femi Kayode Agboola and Adeyinka Afolayan

Influence of nitrogen application on grain yield and end use quality in segregating generations of bread wheat (Triticum aestivum L)

June 2008

  Variation in bread making quality of wheat (Triticum aestivum L) is a major consideration for suppliers and bakers. Grain protein concentration, protein quality, ash content and carbohydrates are major quality attributes of bread wheat. Breeders and their cereal chemist colleagues use predictors of these end use quality factors in the early generations to develop improved cultivars. Six populations of bread...

Author(s): Bayoumi, T. Y and I. S. El- Demardash

Hepatoprotective effect of Cichorium intybus on CCl4-induced liver damage in rats

June 2008

  The purpose of this study was to evaluate the hepatoprotective activity of hydroalcholic extract of Cichorium intybus (Cichorium intybus) using a carbon tetrachloride (CCl4) - induced liver injury in rats. The leaf extract at oral dosage of 200, 400 and 500 mg/kg exhibited significant (P<0.05) protective effect against CCl4 induced hepatoxocity. Level of serum markers such as aspartate...

Author(s): Sadeghi Heibatollah, Nikbakht Mohammad Reza; Ghaitasi Izadpanah and Sabzali Sohailla

Hepatotoxic and hemolytic effects of acute exposure of rats to artesunate overdose

May 2008

  Hepatotoxic and hemolytic effects of artesunate overdose were examined in rats. Forty (40) rats were grouped randomly into four designated as A, B, C, and D. and were given oral administration of artesunate as follows: 0 mg/kg (control), 1 mg/kg (Under-dose), 2 mg/kg (Normal dose) and 4 mg/kg (Overdose) respectively.  The administration was continued for 5 days. Hepatotoxicity was monitored in the rats...

Author(s): Omotuyi I. O, Nwangwu S. C, Okugbo O. T, Okoye O. T, Ojieh G. C and Wogu D.M

Effect of aerobic pre-treatment on production of hydrolases and volatile fatty acids during anaerobic digestion of solid sisal leaf decortications residues

May 2008

  The effect of aerobic pretreatment on the production of hydrolases and volatile fatty acids during anaerobic digestion of solid sisal decortications leaf residue (SLDR) was investigated. Batch solid waste bioreactors with working volume of 2 litres were used in this study.  Batch loads of aerobically treated or untreated sisal leaf residue inoculated with activated sludge mixed culture were packed into...

Author(s): Anthony Manoni Mshandete, Lovisa Björnsson, Amelia Kajumulo Kivaisi, Mugassa Steven Thomas Rubindamayugi and Bo Mattiasson

A comparative study on the atheroprotective potential of heparin and atorvastatin in hypercholesterolemic rats

May 2008

  The present study is to evaluate the effect of standard drug heparin and atorvastatin on the atherogenic disturbances in hypercholesterolemic atherogenic animals. Six groups of male Wistar rats were employed in this study, of which three groups received atherogenic CCT diet (rat chow supplemented with 4% cholesterol, 1% cholic acid and 0.5% thiouracil) for 17 days. Two groups were treated with...

Author(s): Vijayabaskar P, Sethupathy S and Somasundaram S. T

Oxidative injury and enzymic antioxidant misbalance in schizophrenics with positive, negative and cognitive symptoms

April 2008

  With its hallucinations, delusions, thought disorder, and cognitive deficits, schizophrenia affects the most basic human processes of perception, emotion, and judgment. Evidence increasingly suggests that schizophrenia is a subtle disorder of brain development and plasticity and oxidative injury contributes largely to pathophysiology of schizophrenia, indicated by the increased lipid peroxidation products in...

Author(s): Uma Devi P and Chinnaswamy P

Characterization of phospholipase A2 (PLA2) from Echis ocellatus venom

April 2008

  Phospholipase A2 (EC. 3.1.1.4) was isolated and partially characterized from the venom of Echis ocellatus. The enzyme was purified 13.5-fold with a yield of 86.69% on DEAE-Sephadex G-75 column. The PLA2 from E. ocellatus venom had broad pH and temperature ranges with optima of 7.5 and 40oC respectively.  Initial velocity studies for the determination of kinetic constants with...

Author(s): Sallau, A. B., Ibrahim, M. A., Salihu, A. and Patrick, F. U.

Decreased cardiovascular risk and resistance to hyperlipemia-induced hepatic damage in rats by aqueous extract of Urtica dioica

April 2008

  Hyperlipemia and Hepatic metabolism was studied in hyperlipemic albino rats maintained on a high fat diet. Aqueous extract (100, 200, 300mg/kg/day) of Urtica dioica corrected dyslipidemia and restored hepatic chemistry in hyperlipemic animals. The extract was effective in normalizing the atherogenic lipoprotein phenotype. Total cholesterol (CHOL), Triglyceride (TG), Low density lipoprotein...

Author(s): Alisi C. S, Emejulu A. A, Alisi P. N. C, Nwaogu L. A and Onyema O. O

RNA interference: a pathway to drug target identification and validation in trypanosome

March 2008

    RNA interference (RNAi) is a phenomenon through which double stranded RNA (dsRNA) induces potent sequence-specific degradation of homologous transcripts. Besides its function in cellular defense and developmental regulation, it has emerged as an invaluable tool for elucidation of gene function and drug target validation. This is particularly useful when substantial genome sequence data are...

Author(s): Owino, A. Vincent. , , Masiga, K. Daniel , Limo, K. Moses.

Study of the immunomodulatory and anti-inflammatory effects of evening primrose oil in adjuvant arthritis

March 2008

  The present study was carried out to evaluate the anti-arthritic nature of evening primrose oil (EPO) rich in γ-linolenic acid (GLA), on adjuvant-induced arthritic rats. Results were compared to those of diclofenac sodium, a reference standard non-steroidal anti-inflammatory drug. Arthritis was induced by subcutaneous injection of complete Freund’s adjuvant (CFA) in the right hind paw of male...

Author(s): Manal F. Ismail, Shohda A. El-Maraghy, Nermin A.H. Sadik

Diethylnitrosamine-induced hepatocarcinogenesis in rats: possible chemoprevention by blueberries

March 2008

  Nitrosamine compounds are known hepatic carcinogens. This study was designed to study the efficacy of dietary supplementation with blueberries (BB) on diethylnitrosamine (DEN)-initiated hepatocarcinogenesis in male wistar rats. Rats were divided into three groups. The first group served as normal control group, the second group received DEN at a dose of 10 mg/kg body weight five times a week for 15 weeks. The...

Author(s): Nermin A.H. Sadik, Shohda A. EL-Maraghy and Manal F. Ismail

Effects of intraperitoneal administration of vitamins C and E or A and E combinations on the severity of Trypanosoma brucei brucei infection in rats

March 2008

  The effects of intraperitoneal administration of combinations of vitamins C and E (100mg/Kg b.w each) or vitamins A and E (10,000i.u/Kg, 100mg/Kg b. w., respectively)  on Trypanosoma brucei brucei  infection in rats were compared. The rats were infected intraperitoneally with the same parasite load and the experiment lasted for 17 days. The two vitamin combinations significantly (P<0.05) and...

Author(s): Umar, I. A. Rumah, B. L., Bulus, S. L., Kamla, A. A., Jobin, A., Asueliman, B. I., Mazai, M.H., Ibrahim, M. A. and Isah, S.

Mineral and phytochemical contents in leaves of Amaranthus hybridus L and Solanum nigrum L. subjected to different processing methods

February 2008

Mineral and phytochemical contents of leaves of Amaranthus hybridus L. andSolanum nigrum L. Subjected to different processing methods were evaluated. Processing procedures adopted include shredding, sun–drying, oven–drying, steaming and a combination of these. Minerals examined are Na, K, Ca, Mg, Fe, P, and Zn while the phytochemicals are alkaloids, flavonoids, hydrocyanic acid, phenols,...

Author(s): Akubugwo I. E, Obasi N. A, Chinyere G. C and Ugbogu A. E

Trypanocidal potential of methanolic extract of Bridelia ferruginea benth bark in Rattus novergicus

February 2008

    Full Length Research Paper   Trypanocidal potential of methanolic extract of Bridelia ferruginea benth bark in Rattus novergicus   J. T. Ekanem1, *O. M. Kolawole2, O. C. Abbah1   1Department of Biochemistry, Faculty of Basic Health Sciences,...

Author(s): J. T. Ekanem, O. M. Kolawole, O. C. Abbah

Antihepatotoxic potential of Sargassum polycystum (Phaeophyceae) on antioxidant defense status in D-galactosamine-induced hepatitis in rats

February 2008

We have examined the protective effect of ethanol extract of Sargassum polycystum on hepatic antioxidant status in D-galactosamine-induced hepatitis in rats. Levels of diagnostic marker enzymes [alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK)] in plasma, lipid peroxides, reduced glutathione and the activities of glutathione...

Author(s): B. Meena, R. Anbin Ezhilan, R. Rajesh, A. Sheik Hussain, B. Ganesan, R. Anandan

Relationship between somatic cell counts, dairy cattle milk yield and composition in Burkina Faso

February 2008

The study was carried out on ten local dairy cows called Zebu at a research centre located in the west part of Burkina Faso. Dairy cows were supplemented 53.8 MJ metabolized energy and 735.7 g Crude Protein and were free access to natural pasture. Milk yield recording and individual milk sample collection took place within one month. Milk samples were analyzed for composition with the method of mid infrared spectroscopy...

Author(s): Ouédraogo G. A, Millogo V, Anago-Sidibé A. G and Kanwé B. A

Hepatoprotective activity of Bi - herbal ethanolic extract on CCl4 induced hepatic damage in rats

February 2008

The combined hepatoprotective effect of Bi- herbal ethanolic extract (BHEE) was evaluated against carbon tetra chloride (CCl4) induced hepatic damage in rats. Ethanolic extract from the leaves of Eclipta alba and seeds of Piper longum at a dose level of 50 mg/kg body weight was administered orally daily once for 14 days. The substantially elevated serum marker enzymes such as SGOT, SGPT, ALP, LDH,...

Author(s): Samudram P., Rajeshwari Hari, Vasuki R., Geetha A. and Sathiya Moorthi P.

Effects of lipids and (n-3) highly unsaturated fatty acids compositions of three artificial foods on survival, growth and body composition of common dentex’s fingerlings (Dentex dentex, L)

January 2008

Dentex juveniles of initial live weight 5 - 6 g and 108 days old after hatching, were fed three different diets for ten weeks (moist pellet (MP), imported dry pellet (IDP) and locally dry pellet (LDP)) containing three different lipid levels (26, 21 and 10% respectively) and crude protein levels (46, 45 and 47% respectively). Fatty acid composition showed that, DHA and EPA contents of moist pellet are...

Author(s): Ali Ait Ali, Nadia Rharbi, Azeddine Abrehouche and Hassan Nhhala

Gangliosides as potential inhibitors of Naja naja venom PLA2 (NV-PLA2) induced human erythrocyte membrane damage

January 2008

We determined the ability of mixed gangliosides (16% GD1b, 19% GT1b, 21% GM1, and 40% GD1a) and individual gangliosides GM1 and GD1b to modulate the NV-PLA2 induced human erythrocyte ghost membrane damage. CM-Sephadex purification of crude Naja naja venom yielded eight peaks of which peak VII, a major phospholipase A2 (NV-PLA2) accounted for 22% of the total protein recovered and 8% of the total...

Author(s): Mukunda Chethankumar and Leela Srinivas

Trans-resveratrol as phenolic indicator of somatic embryogenesis induction in cotton (Gossypium hirsutum L.) cell suspensions

January 2008

Trans-resveratrol is a phytoalexin which was found in some grapes and in many other plants. This phenolic compound has gained much attention, as it was indicated to be associated with fungus and disease resistance. Also, it has been shown that phenols compounds could play an important role in somatic embryogenesis induction of various plants. In cotton, embryogenic structures take place in cell suspension cultures....

Author(s): Kouakou Tanoh Hilaire, , Koné Mongomaké, Koné Daouda, Kouadio Yatty Justin, Amani N’guessan Georges, Téguo Waffo Pierre, Décendit Alain and Mérillon Jean-Michel

Allethrin-induced biochemical changes and properties of human erythrocyte membrane

January 2008

Erythrocyte membranes from twelve human volunteers exposed regularly to allethrin, a mosquito repellent of type-I pyrethroid, were analyzed for cholesterol (C), phospholipids (P), and individual phospholipid classes to assess changes induced by this toxicant. A decrease in C and P moieties with no change in C: P ratio was observed with allethrin exposure. A significant reduction in the amount of phosphotidyl serine (PS)...

Author(s): Narendra M., Kavitha G., Padmavathi P., Helah Kiranmai A. and Varadacharyulu N. C.

Aeration as a factor in textile dye bioremoval by Aspergillus niger

January 2008

Experiments were done to study the bioremoval/ biosorption of dis-azo dye byAspergillus niger strain 20 in two concentrations using 5 liter bioreactor at five aeration rates. The experimental results are compared for various operating conditions. The dye used was direct brown and the inlet air flow rate was: 1/8, ¼, ½, 1, 2 v/v/min. The aeration rate of ½ v/v/min yielded 9.2 g fungal...

Author(s): Wafaa M. Abd El-Rahim, Ola Ahmed M. El-Ardy and Hassan Moawad

Status of the levels of lead and selected trace elements in type 2 diabetes mellitus patients in Abeokuta, Nigeria

December 2007

      The study investigated the status of lead and selected mineral elements (copper, zinc and chromium) in type-2 diabetes mellitus patients, so as to establish the possible interactive connections of these metals with the disease and with each other in diabetic patients. Thirty-one patients (15 male and 16 female), clinically diagnosed for...

Author(s): Babalola O. O, Ojo L. O and Akinleye A.O

Molecular studies on the immobility of genomic construction in the random mating population

December 2007

      Random population studies are very important to estimate the environmental factors in nutritional, physiological and breeding experiments. Studying the quantitative traits, consisting of genetic, environmental factors and interaction between them is very important as well. In a random population study, the genetic variation for 50 chicken individuals...

Author(s): A.E.Elkomy, Hanan.H.Ghanem and Elsayed E. Hafez

Phenolic content and phospholipids peroxidation inhibition by methanolic extracts of two medicinal plants: Elionurus muticus and Hypoxis hemerocallidea

December 2007

      Two commonly known medicinal plants from Zimbabwe were analyzed for total phenolic content, superoxide radical scavenging activity and the ability to inhibit lipid peroxidation in rat brain extracts. The plant samples used in this study were Elionurus muticus and Hypoxis hemerocallidea. The Folin Ciocalteu method was used to determine the...

Author(s): Dzingirai, B. , Muchuweti, M , Murenje, T., Chidewe, C., Benhura, M.A.N and Chagonda, L. S.

Determination of the glycemic index of steamed cakes using two different legumes 'bambara nut (Vigna subterranean) and cowpea (Vigina unguiculata)'

December 2007

  This study was carried out to determine the blood glucose response and glycemic index of food for diabetics and non-diabetics. The subjects comprised of 10 diabetics and 7 non-diabetics. The results of the physical characteristics showed that the diabetic subjects were on the average older (age bracket between 51 and 70years) than the non-diabetics (18 and 23years). The Body mass index (BMI) values indicated...

Author(s): Onimawo I. A., Ijeh I., Ukoha U. and Nwachukwu G. T.

Influence of Cassia auriculata flowers on insulin receptors in streptozotocin induced diabetic rats: studies on insulin binding to erythrocytes

December 2007

  In this paper, we investigated the insulin-receptor-binding effect of Cassia auriculata flower extract (CFEt) in streptozotocin induced male wistar rats, using circulating erythrocytes as a model system. We measured the levels of plasma glucose and plasma insulin and the binding of insulin to cell-membrane erythrocyte receptors (ER) in CFEt and glibenclamide treated diabetic rats. The mean specific...

Author(s): L. Pari , P. Murugan and C. Appa Rao

Evaluation of biochemical indices of male rat reproductive function and testicular histology in wistar rats following chronic administration of aqueous extract of Fadogia agrestis (Schweinf. Ex Heirn) stem

December 2007

  The effects of administration of aqueous extracts of Fadogia agrestis stem for 28 days and the 10 days post treatment effects on biochemical indices of reproductive function and testicular histology were evaluated in male albino rats. The rats were randomly selected into four study groups, A, B, C and D of 10 rats per group. Animals in group A (control) received 1 ml of distilled water (vehicle),...

Author(s): Yakubu, M. T., Oladiji, A. T. and Akanji, M. A.

Lavudine therapy and hepatotoxicity as seen in a Nigerian tertiary antiretroviral treatment centre

November 2007

      Hepatic toxicity is a common complication of anti-retroviral treatment in HIV patients, usually indicated or heralded by the elevation of liver transaminases measured in the blood. There had been reported evidence of hepatic toxicity in all the three currently approved classes of anti-retroviral drugs. However, its severity in some cases...

Author(s): Akande A.A, Olaosebikan O.F, Jimoh A.K, Abdulazeez, Olawumi H.O

Quantitation of alcohols in orange wine fermented by four strains of yeast

November 2007

  Gas liquid chromatography was used to identify the types of alcohol present in wines produced by fermenting orange juice with four strains of yeast; Saccharomyces cerevisiae (isolated from yam), S. cerevisiae (from sugarcane molasses),Saccharomyces carlsbergensis (from sugarcane molasses) and S. cerevisiae var. ellipsoideus (from orange juice). Ethanol and methanol were the...

Author(s): Okunowo, Wahab Oluwanisola and Osuntoki, Akinniyi Adediran

Effect of Emblica officinalis against alcohol-induced biochemical changes in plasma and red blood cells of rats

November 2007

  Alcohol is widely abused as a psychoactive drug. Emblica officinalis is known for its therapeutic effects and the present study is aimed at investigating therapeutic efficacy of aqueous extract of Emblica officinalis (AEEO) against alcohol-induced damage. Thirty three percent (v/v) alcohol (10 g/kg body weight) and AEEO (250 mg/kg body weight/day) was orally administered once a day for 60...

Author(s): Damodara Reddy. V, Saayi Krushna G, Padmavathi. P and Varadacharyulu. N.Ch

Biochemical characterization of two α -mannosidases from breadfruit (Artocarpus communis) seeds

November 2007

  The importance of α -mannosidases in glycoproteins processing and their application in food and pharmaceutical industry led us to further explore plant α -mannosidases. Thus, two α -mannosidases were purified from matured breadfruit (Artocarpus communis) seeds, by successive chromatography on Diethylaminoethyl-Sepharose CL-6B and Sephacryl S-200 HR to apparent...

Author(s): Amedée Pascal Ahi, Jean Tia Gonnety, Betty Meuwiah Faulet, Lucien Patrice Kouamé and Sébastien Niamké.

Protective effect of betaine on changes in the levels of protein, glycoproteins and amino acids in isoprenaline-induced myocardial infarction in rats

November 2007

      The protective effect of betaine administration on changes in the levels of protein, glycoproteins and amino acids was studied in isoprenaline-induced myocardial infarction in rats as an animal model of myocardial infarction in man. Oral pre-treatment with betaine significantly (P<0.05) attenuated the isoprenaline-induced rise in the levels of troponin-T...

Author(s): B. Ganesan, R. Rajesh, , R. Anandan , and N. Dhandapani,

Garcinia kola extract reduced cisplatin-induced kidney dysfunction in rats

November 2007

  The effect of Garcinia kola extract on cisplatin-induced renal insufficiency in rats was studied. A total of fifteen rats were used for the study and were split into three groups of five rats per group. Rats in group I were normal controls. Renal insufficiency was induced in rats of groups II and III by a single intraperitoneal administration of cisplatin (5 mg/kg b.w.). After three days, rats in...

Author(s): Okoko Tebekeme and Awhin Ejiro Prosper

Impact of processing technique on the apparent bioavailability of cooking banana (matooke) starch

October 2007

  The aim of the study was to compare the apparent bioavailability of starch in raw, cooked and extruded matooke flours using weanling mice. Two control and three test groups consisting of seven mice each (initial body weight of 21.32 ± 1.05 g) were fed diets incorporating soluble unavailable starch (Control I), soluble starch (Control II) and raw, cooked and extruded matooke flours as carbohydrate...

Author(s): Florence Isabirye Muranga, Harini Sampath, Judith A. Marlett and James M. Ntambi,

N-nitrosodimethylamine induced changes in the activities of carcinogen-metabolizing enzymes in the liver of male mice: role of glutathione and gossypol as antioxidants

October 2007

  The importance of nutrition in protecting living organisms from the toxic effects of environmental carcinogens has attracted the attention of many researchers. In order to exert their toxic and/or carcinogenic effects, most carcinogens need to be activated primarily by phase I drug-metabolizing enzymes including; cytochrome P450, cytochrome b5, arylhydrocarbon hydroxylase (AHH), N-nitroso-dimethylamine...

Author(s): Sheweita S. A., Mousa N. and Newairy A. A.

Biochemical assessment of the effects of crude oil contaminated catfish (Clarias gariepinus) on the hepatocytes and performance of rat

October 2007

      Biochemical investigations were carried out to assess the effect of consumption of crude oil contaminated catfish on the hepatocytes and performance of rat. Catfish (Clarias gariepinus) (n = 120) were grouped into 6 of 20 catfish each and held for 30 h in 5 different mixtures of crude oil polluted water (0.1, 0.25, 0.5, 0.75 and 1% v/v). Catfish in the...

Author(s): T. O. Sunmonu and O. B. Oloyede

Physicochemical and microbiological characteristics of optimized and traditional shea butters from Côte d’Ivoire

September 2007

  A comparison was realized between the characteristics of shea butters from local markets in Côte d’Ivoire and optimized shea butters, manufactured in a laboratory, in order to valuate the improvements in quality. Large diversity concerning the colour (beige, grey and yellow), odour (odourless, moderate, fragrant and rancid) and the consistency (soft and hard) was found for the ordinary shea butter...

Author(s): Rose-Monde Megnanou , Sébastien Niamke and Jacques Diopoh

Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes

September 2007

  Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oat-spelt xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1 was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a...

Author(s): Okafor, U. A., Emezue, T. N., Okochi, V. I. , Onyegeme-Okerenta, B. M.  and Nwodo-Chinedu, S. 

Sickle-cell anaemia in Nigeria: dermatoglyphic analysis of 90 cases

September 2007

  Dermatoglyphic analysis of 90 Sickle-cell Anaemia cases and 90 normal subjects was carried out in this study.  It involved the digital patterns, ATD angle, A – B ridge count, axial triradius, digital triradius and palmar crease on the hands. 58.44% of the digital patterns in sickle-cell cases were ulnar loop as against 60.14% in the normals. The percentage of Whorl, arch and radial loop in...

Author(s): Oladipo GS, Olabiyi O, Oremosu AA, Noronha CC, Okanlawon AO and Paul CU.

Biological responses of injured human skin fibroblasts to assess the efficacy of in vitro models for cell stress studies

September 2007

  The classical central scratch method has been used to successfully induce a wound environment however this model has been criticized. This study aimed to compare the cellular responses of normal fibroblast cells with those of wounded, diabetic wounded, acidic and hypoxic cells to determine if the latter two models are more effective in inducing an injured environment than the central scratch method. The...

Author(s): Innocent Lutho Zungu, Denise Hawkins Evans, Nicolette Houreld and Heidi Abrahamse

Suppression-subtractive hybridization: A rapid and inexpensive detection methodology for up-regulated Perkinsus olseni genes

August 2007

  A cDNA forward subtraction library was constructed from the mollusc parasite Perkinsus olseni exposed to hemolymph from its natural host, the clam Ruditapes decussatus, and two different methodologies were used to unravel different non-redundant contigs. Our results demonstrated that screening of the non-enriched direct cDNA subtractive library (Dfsl) was the most efficient and least time-...

Author(s): Ascenso R. M. T., Leite R. B., Afonso R., Cancela M. L.

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