African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 384

AJBR Articles

December 2011

Alterations in the biochemical components and photosynthetic pigments of mulberry (Morus Spp.) attacked by leaf – roller (Diaphania pulverulentalis) pest

Leaf roller (Diaphania pulverulentalis Hampson) is a serious pest of mulberry (Morus alba L.), which is the sole food for silkworm – Bombyx mori L. It attacks the tender leaves of the host causing considerable damage which alters the leaf quality. An attempt was made to know the changes in the biochemical components (free amino acids, total soluble proteins, total reducing sugars, total soluble...

Author(s): A. Mahadeva and V. Nagaveni

  • Article Number: 76C9F1A12954

December 2011

The apoptotic and cytotoxic effects of Polygonum avicular extract on Hela-S cervical cancer cell line

Cervical cancer is the second main cause of cancer leading death in women. Therefore, targeting it, preferably with natural compounds derived from medicinal plant such as Polygonum avicular as an herbal extract have astringent properties. Herbal medicines in all regions of the developing world are widely used. Unlike the heavy use, there are very little information about the health and efficiency of the treatment by...

Author(s): Rahmati Mohammad, Bannazadeh Hossein, Fazli Davood, Tahmasebzadeh Farnaz, Farrokhi Ali and Rasmi Yusef

  • Article Number: EB92BD812962

November 2011

Anti-atherogenic effects of supplementation with vitamin B6 (Pyridoxine) in albino rats

Elevation of serum lipids have been implicated to predispose to cardiovascular disorders which includes hypertension, stroke, atherosclerosis etc. Various studies have reported links between supplementation with vitamin B complex and reduction in oxidative stress and inflammatory reactions, two events associated with cardiovascular disorders. Data on possible direct regulation on lipid profile by vitamin B6 is very...

Author(s): Adekunle Adeniran S. and Adedeji Adebayo L.

  • Article Number: DEDA70112912

November 2011

Dyslipidemic and atherogenic effects of academic stress

Evidence suggests that there is strong relationship between stress and development of cardiovascular disorders. Here, the relationship between academic stress and selected traditional markers of cardiovascular disorder such as lipids and lipoprotein profile and apoproteins were investigated. Eighty apparently healthy male and female students participated in the study. Plasma concentration of selected biochemical...

Author(s): Adekunle Adeniran S.

  • Article Number: 0B0D0A212921

October 2011

Anti-malarial activity of cocoa powder in mice

At present, malaria is one of the most important parasitic diseases in the world killing more than one million people annually. It is an important public health problem because many of the drugs that are being prescribed for the treatment of malaria have become ineffective to the disease. This study was therefore carried out to determine the anti-malaria activity of cocoa powder through the use of mouse model. Natural...

Author(s): Jayeola, C. O., Oluwadun, A., Olubamiwa, O., Effedua, H. I. and Kale, O. E

  • Article Number: 20EF26B12866

October 2011

Oxidative stress biomarkers in young male rats fed with stevioside

Stevioside is a natural non-caloric sweetener refined from Stevia rebaudiana Bertoni leaves. The introduction of stevioside as a sugar substitute in the diets of diabetics and others on carbohydrate-controlled diets has been suggested, but safety issues have prevented implementation. The aim of this study was to examine antioxidant status changes in the sera, livers and kidneys of young male rats fed with low...

Author(s): Hala A. Awney

  • Article Number: DEEB19912878

October 2011

Screening of antioxidant activity, total phenolics and gas chromatograph and mass spectrometer (GC-MS) study of delonix regia

The present study was carried out for identification of the phytochemicals present in the Delonix regia leaves and also evaluates the total phenols, total flavonoids and antioxidant activity of the leaf extract. Total phenols were carried out by Folin Ciocalteu method and the phenolic content was 16.00 mg/100 g of gallic acid equivalent (GE). Antioxidant activity was evaluated by 2,2-Diphenyl-1-Picrylhydrazyl...

Author(s): P. Maria jancy Rani, P. S. M. Kannan and S. Kumaravel

  • Article Number: 1972CF912890

October 2011

Antioxidant activity of methanolic extract of Pongamia pinnata on lead acetate induced hepatic damage in rats

The antioxidant effect of methanolic extract of Pongamia pinnata was evaluated against lead acetate induced hepatic damage in male albino rats. Methanolic extract from the flowers of P. pinnata at a dose level of 150 mg/kg b.wt/day was administered orally daily once for 90 days. The substantially decreased enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione...

Author(s): R. Anuradha and Palaniyandi Krishnamoorthy

  • Article Number: 6E3DB9112899

September 2011

Influence of the bioinsecticides, NeemAzal, on main body metabolites of the 3rd larval instar of the house fly Musca domestica (Diptera: Muscidae)

The present study was designed and conducted to determine the effects of Neemazal on the general body metabolism of the house fly Musca domestica. The second instar larvae of the house fly M. domestica (L.) were reared on artificial diet treated with the botanical extract, NeemAzal. Five concentration levels of this compound (1000, 500, 250, 125 and 62.5 ppm) were used. The main body metabolites,...

Author(s): Mohammad A. Kassem, Tarek A. Mohammad and Ahmad S. Bream

  • Article Number: 6AE708A12694

September 2011

Changes in liver and serum transaminases and alkaline phosphatase enzyme activities in Plasmodium berghei infected mice treated with aqueous extract of Aframomum sceptrum

One of the effects of Plasmodium infection which causes malaria is the invasion of the hepatocyte which affects liver and blood transaminases activities. This study was design to examine the hepato-protective effect of Aframomum sceptrum in mice infected withPlasmodium berghei. Thirty-six abino male mice infected (test) and non infected (control) of 8 weeks old were used for this research. The...

Author(s): George, B. O., Osioma, E., Okpoghono, J. and Aina, O. O.

  • Article Number: BDB434C12714

September 2011

Effect of highly active anti-retroviral therapy (HAART) on lipid profile in a human immunodeficiency virus (HIV) infected Nigerian population

Nigeria remains the country with the third highest number of human immunodeficiency virus (HIV) infected individuals with over 600,000 HIV-infected subjects requiring antiretroviral therapy (ART). Since there is little or no data on the effect of highly active antiretroviral therapy (HAART) on the lipid profile of HIV-infected subjects in Nigeria, this study was aimed at evaluating the dyslipidaemia associated with...

Author(s): Francis M. Awah, and Onyinye Agughasi

  • Article Number: ACD40C212734

September 2011

Effects of 4-nonylphenol on metabolic enzymes, some ions and biochemical blood parameters of the African catfish Clarias gariepinus (Burchell, 1822)

In present work, some biochemical characteristics of the catfish, Clarias gariepinus were studied under the effect of different sublethal doses of 4-nonylphenol (0, 0.05, 0.08 and 0.1 mgl-1). Liver enzymes ALT and AST increased insignificantly at P<0.05 whereas the ALP decreased insignificantly. The activities of G6PDH showed significant increase with the increased sublethel doses of 4-nonylphenol...

Author(s): Alaa El-Din H. Sayed, Imam A. A.  Mekkawy, and  Usama M. Mahmoud

  • Article Number: DDE089412747

September 2011

Deficiency of vitamin B12 among Jordanian people with psychological and biological activity

This study was conducted to evaluate vitamin B12 level among Jordanians healthy people and its effects on psychological parameters. One hundred and eighty six subjects were recruited in the study. The subjects were chosen to be healthy, aged between 16 to 71 years. Participants were asked to fill a detailed questionnaire that covered medical data then they were divided into three age groups. Serum vitamin B12 levels...

Author(s): Mohammad A. Al-Fararjeh, Nader Jaradat and Abdulrahim Aljamal

  • Article Number: B5E4BD712762

September 2011

Reduction of platelet and lymphocyte counts and elevation of neutrophil counts in rats treated with aqueous leaf extract of Ocimum gratissimum

Ocimum gratissimum (Linn.) has been widely used for food and medicinal purposes.  The effect of aqueous leaf extract of O. gratissimum on haematological parameters of albino Wistar rats was studied.  Acute toxicity study showed a median lethal dose (LD50) value of 2121.32 mg/kg body weight for intraperitoneal (i. p) route in mice. Twenty rats’ weights ranging between 85 to 115 g...

Author(s): Okon U. A., Ita S. O., Ekpenyong C. E., Davies, K. G. and Inyang, O. I.

  • Article Number: 8430E4E12777

September 2011

Chelating ability of sulbactomax drug in arsenic intoxication

The aim of this study was to determine the chelating ability of Sulbactomax drug in arsenic pre-exposed rats. 24 rats were divided into I to III groups. Group I was control while Groups II to III were arsenic exposed and arsenic plus Sulbactomax treated group. Arsenic toxicity was induced via intraperitoneally administration of arsenic trioxide (As2O3) 10 mg/kg body weight/day for three weeks. Toxicity...

Author(s): V. K. Dwivedi, A. Arya, H. Gupta, A. Bhatnagar, P. Kumar and M. Chaudhary

  • Article Number: 33B4C3912838

September 2011

Nerve growth factor (NGF) combined with oxygen glucose deprivation (OGD) induces neural ischemia tolerance in PC12 cells

Ischemic cerebrovascular disease is a major disease in humans. To better study this disease, a good ischemia model of nerve cells is needed. Nerve growth factor (NGF) could induce PC12 cells to become neurons. Oxygen glucose deprivation (OGD) could lead to hypoxia and neuronal ischemia. In this study, we used NGF and OGD to stimulate PC12 cells and converted them into neurons in order to establish an ischemia model....

Author(s): Chunli Mei, Jinting He, Jing Mang, Guihua Xu, Zhongshu Li, Wenzhao Liang and Zhongxin Xu

  • Article Number: EC64E7012847

September 2011

Kinetic study of different flavonoids as inhibitors of beta-lactamase enzyme

The phenotypic study of the clinical strain Escherichia coli K18 showed that it is resistant to penicillin and amoxicillin-clavulanate, but susceptible to the combination of piperacillin-tazobactam and the cephalosporins 3rd generation and meropenem. The isolated β-lactamase, using ion-exchange chromatography on resin Mono-Q, gave a yield of 56%, a purification factor equal to 5.9, a molecular weight...

Author(s): BOUSSOUALIM, Naoual, MEZIANE-CHERIF, Djalal and BAGHIANI, Abderrahmane

  • Article Number: 17E08CB12857

August 2011

In vitro biochemical investigations of the effects of Carica papaya and Fagara zanthoxyloides on antioxidant status and sickle erythrocytes

Various works have identified a number of herbal applications that have ameliorating effects on sickle cell disorders. The antisickling activities of dried Carica papaya leaves and roots of Fagara zanthoxyloides are being investigated in this study to determine the antioxidant properties of the plant extracts and their effects on homozygous sickle cell (SS) erythrocytes in vitro. The...

Author(s): Imaga, Ngozi O. A., Shaire Esther A., Ogbeide Samson and Samuel K. Akindele

  • Article Number: 43A5B6612586

August 2011

Biochemical effects of drinking Terminalia catappa Linn. decoction in Wistar rats

The biochemical effects of drinking Terminalia catappa Linn. decoction in place of water using weanling wistar rat (Rattus norvegicus) models of both sexes was studied. In folklore, the decoction is taken as a medicine by sicklers. Some of the phytochemical and chemical constituents detected in the decoction included  tannins, flavonoids, saponins, β-carotene, thiocyanates, cardiac glycosides,...

Author(s): Ibegbulem, C. O., Eyong, E. U. and Essien, E. U.

  • Article Number: 2BDE2CA12598

August 2011

Effects of weaning formulae and electrolyte quality of water on rats administered with contaminated water sources

The study was aimed at correlating the protein quality of weaning formula with the amelioration of heavy metal toxicity. Wistar albino rats were variously administered borehole water samples, Aba River water samples, simulation experiment (deionized water incorporated separately with lead, cadmium and arsenate) water samples (negative control) and deionized water (positive control). Two weaning formulae viz: SGMC...

Author(s): Obimba, Kelechukwu Clarence, Ijeh, Ifeoma Irene and Okafor, Nnacheta Polycarp

  • Article Number: DB2ADC312620

August 2011

Toxicological and antioxidant effects of ethanolic extract of Baphia nitida on diazepam induced oxidative stress in rats

The toxicological and antioxidant effects of the ethanolic extracts of Baphia nitida on diazepam induced oxidative stress in rats were investigated. Forty Sprague Dawley albino rats divided into eight groups of five animals each were employed in the study. Group one or control was not administered diazepam or ethanolic extract of B. nitida but received saline while group two received diazepam only....

Author(s): Akande, I. S., Akande, B. E. and Gbenle, G. O.

  • Article Number: 4BC128912633

August 2011

Changes in physiological and biochemical indicators associated with salt tolerance in cotton, sorghum and cowpea

The aim of this study was to evaluate the interaction between salinity, growth, and enzymatic antioxidants in cotton, cowpea and sorghum. Salt stress significantly reduced plant growth, especially in cowpea. Na+ and Cl- concentrations increased in leaves and roots of these three species, especially for cotton at 4.0 and 8.0 dS/m, in relation to control. Salinity significantly increased...

Author(s): Valdinéia Soares Freitas, Nara Lídia Mendes Alencar, Claudivan Feitosa de Lacerda, José Tarquinio Prisco and Enéas Gomes-Filho

  • Article Number: 42A58B512649

July 2011

Angiotensin-converting enzyme genotypes relationship with blood pressure, C-reactive protein and selected physical tests in Zulu South African cricketers

Zulu cricketers (n=14) and students (n=17) as controls were genotyped (blood spots) for angiotensin converting enzyme (ACE), gene by PCR amplification followed by agarose gel electrophoresis. Systolic and diastolic blood pressure (SBP and DBP) and grip strength (kg), knee extension and flexion (Nm/kg) were measured, systolic tension time (STT) and metabolic rates (MR) were calculated. After ANOVA, the association...

Author(s): Trayana Djarova, Abigail Ramakoaba, Albert Basson, Sam Mugandani, Jeanne Grace, Jacque Cloete and Gregory Watson

  • Article Number: 1179B2E12536

July 2011

Altered protease activity due to heat shock in the whole organism Bombyx mori L.

The domesticated insect Bombyx mori respond well to environmental fluctuations that govern the success of cocoon production due to its piokilothermic nature. Since, the interaction between heat shock proteins and proteases in the whole organism that complete larval life in natural environment remain obscure, we have employed zymography technique to examine different classes of proteases and its activity under...

Author(s): Rajesh R. K., Manjunatha H. B. and Aparna H. S.,

  • Article Number: C37DB2C12549

July 2011

Anti-plasmodial activity of aqueous root extract of Acacia nilotica

Acacia nilotica, of family Fabaceae, is a thorny tree commonly used in Northern Nigeria for the treatment of cough, diabetes and malaria. Aqueous root extract of A. nilotica was analyzed for antiplasmodial activity in mice. Acute toxicity of the extract was studied using Organization for Economic Cooperation and Development (OECD) guideline 423. Suppressive activity, curative and prophylactic effect...

Author(s): Alli L. A., Adesokan A. A., Salawu O. A., Akanji M. A. and Tijani A. Y.

  • Article Number: DC0CBF512553

July 2011

Evaluation of different matrices for production of alkaline protease from Bacillus subtilis –K 30 by entrapment technique

The present investigation evaluates the suitability of different matrices such as calcium alginate, polyacrylamide, agar-agar and gelatin for production of alkaline protease from Bacillus subtilis-K 30 using immobilization approach. Calcium alginate was found to be an effective and suitable matrix for higher alkaline protease productivity compared to other matrices studied. All the matrices were selected for...

Author(s): K. Suresh Babu Naidu, K. Lakshmi Devi and K. Jamila Adam

  • Article Number: 9BA3B3B12566

June 2011

Direct electron transfer of hemoglobin on nickel oxide nanoparticles modified graphite electrode

Direct electron transfer of hemoglobin, immobilized on a nickel oxide nanoparticles modified graphite electrode, was studied. Nickel oxide nanoparticles synthesized by electrochemical methods. The prepared nanoparticles were characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The resulting electrode displayed an excellent redox behavior for the hemoglobin. The hemoglobin...

Author(s): Rezaei-Zarchi S., Imani S, Javid A, Zand A. M., Saadati M. and Zagari Z.

  • Article Number: 06989D312442

June 2011

Investigating structure-activity relationships within Enterococcus faecalis 2-deoxyribose aldolase

The 2-deoxyribose aldolase (DERA) is an enzyme used for the synthesis and decomposition of carbohydrates. The DERA has been shown in previous studies to be utilized in an alternative metabolic pathway during stress. This article uses the Enterococcus faecalis DERA as a model system to study the structure-function relationship of amino acids towards DERA activity in hopes of garnering a better understanding of...

Author(s): Nathan Wymer, Jeremy Steflik and Maria Brown

  • Article Number: 5D1886512458

June 2011

Thermostable, alkaline tolerant lipase from Bacillus licheniformis using peanut oil cake as a substrate

Thermostable alkaline tolerant lipase producing Bacillus licheniformis isolated from marine environment. Peanut oil cake was used as substrate for enzyme production and purified to 3.6 fold with a specific activity of 148.4 U/mg of protein with molecular weight of 35 kDa. The enzyme was 100% stable at 60°C, it retains more than 80% activity after 1 h incubation at 70°C and pH 9-11.5. Metals like Ca2+,...

Author(s): N. Annamalai, S. Elayaraja, S. Vijayalakshmi and T. Balasubramanian

  • Article Number: 1721C3612470

June 2011

Changes in the amino acids contents of selected leafy vegetables subjected to different processing treatments

Changes in the amino acids contents of Veronica amygdaline, Gnetum africana, Gongronema latifolium and Ocimum gratissimum subjected to different processing treatments were investigated. The processing treatments employed include fresh milling, sun drying, oven drying, steaming and a combination of these while the amino acids evaluated include isoleucine, leucine, lysine, methionine, cysteine,...

Author(s): Chinyere, G. C. and Obasi, N. A.

  • Article Number: 0BD7BFC12483

May 2011

a1–Antitrypsin: Anti-inflammatory roles in exercise and atherosclerosis

a1-Antitrypsin, also known as serum trypsin inhibitor, is an acute phase protein that is upregulated in response to tissue damage and infection. More specifically this glycoprotein affords the host protection against enzymes that are released by immune inflammatory cells. The most notable of these enzymes is neutrophil elastase. Neutrophil elastase has the ability to damage vasculature and in doing so may contribute to...

Author(s): Stuart .J. Semple and Andrew .J. McKune

  • Article Number: 772C30912388

May 2011

Variation in antioxidant and aroma compounds at different altitude: A study on tea (Camellia sinensis L. Kuntze) clones of Darjeeling and Assam, India

Plants at high altitude are subject to enhanced oxidative stress due to high UV fluence (resulting from air rarefaction) compared to places at low altitude. For survival, plants have developed specific cell protective compounds viz. the flavonoids. Flavonoid derivatives (viz. flavonoid glycoside) may also serve as precursor of aroma producing compounds and are thus relevant in studies on plant quality under varying UV...

Author(s): Sudeshna Bhattacharya and Swati Sen-Mandi

  • Article Number: 338268312406

May 2011

The effects of mannan-oligosaccharides on cecal microbial populations, blood parameters, immune response and performance of broiler chicks under controlled condition

A study was conducted to evaluate the effects of mannan-oligosaccharides (MOS) on cecal microbial populations, immune responses to phytohaemagglutinin-P (PHA-P) and sheep red blood cell (SRBC) and performance (weight gain and feed conversion ratio) of broiler chicks under strict controlled condition. Sixty four day-old male broiler chicks were randomly assigned to 12 battery cages pens of 4 chicks each and were fed...

Author(s): Saeed Khalaji, Mojtaba Zaghari and Somaye Nezafati

  • Article Number: 85C55BE12418

April 2011

Radio-protective chelating agents against DNA oxidative damage

The present study aims to investigate the strong neuro and radio-protective activity of chelating agents through inhibition of metal catalyzed Fenton reaction and aims to explore the potential of PCR-RFLP as biomarkers. Polymorphisms of rat brain angiotensin II subtype 2 receptor gene (AT2RG) were investigated as a biomarker for the effect of 6 Gy irradiation with or without prior carnosine and dimer­captosuccinic...

Author(s): M. H. Awwad, Samy A. Abd El-Azim, F. A. M. Marzouk, E. A. El-Ghany, and M. A. Barakat

  • Article Number: 885BD0C12287

April 2011

Multipotent mesenchymal stem cells (MSCs) from human umbilical cord: Potential differentiation of germ cells

Previous controversy exists as to whether umbilical cord (UC) can serve as a source of multipotent mesenchymal stem cells (MSCs) with their characteristics. Different methods and reagents have been used to induce the differentiation of UC-MSCs into functional cells. We investigated the isolation of UC-MSCs and their potential differentiation into neurons, cardiomyocytes and germ cells in vitro. The phenotypes,...

Author(s): Jinlian Hua, Pubin Qiu, Haijing Zhu, Hui Cao, Fang Wang, and Wei Li

  • Article Number: 4976B1512316

April 2011

Comparison of peroxidase activities from Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in Burkina Faso

Current applications of peroxidase in various areas of biotechnology and clinical biochemistry show the interest for further screening for peroxidase. Thus, peroxidase activities were screened in higher plants such as Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in a tropical environment. The enzymes were investigated for their specific activities and best...

Author(s): Mamounata Diao, Oumou H. Kone, Nafissétou Ouedraogo, Romaric G. Bayili, Imael H. N. Bassole, and Mamoudou H. Dicko

  • Article Number: 6514EBC12333

April 2011

Levels of two oxidative stress indicators of human sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa

The present in vitro study investigated the levels of oxidative stress indicators, namely, malondialdehyde (MDA) and methaemoglobin (mHb) when sickle erythrocytes were incubated in aqueous leaf extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa for 12 h. At regular time intervals of 3 h, portions of the incubation mixture were withdrawn and spectrophotometric...

Author(s): Paul Chidoka Chikezie

  • Article Number: 3274E9212345

April 2011

Utilization of dietary therapies in the alleviation of protein energy malnutrition in kwashiokor-induced rats

The utilization of diets prepared from available and affordable plants (soya bean, groundnut, maize, and fluted pumpkin green leaves) and animal sources (milk, catfish, and crayfish), in the alleviation of protein energy malnutrition (PEM) was studied, to determine comparative dietary efficiencies of some fortified weaning formulae. The therapeutic diets used in alleviating PEM in the kwashiorkor-induced rats were...

Author(s): Obimba, Kelechukwu Clarence

  • Article Number: 0C8A32E12352

March 2011

Incomplete laboratory request forms as a contributory factor to preanalytical errors in a Nigerian teaching hospital

That most laboratory errors occur in the preanalytical phase has been demonstrated in the past. There is paucity of data on the contribution of incomplete request forms to preanalytical errors in Nigerian hospitals. We examined our laboratory request forms for the frequency of incomplete data. Request forms received at the laboratory in a three- month period were examined for some parameters. The completeness of...

Author(s): Adegoke O. A., Idowu A. A. and Jeje O. A.

  • Article Number: 805252E12208

March 2011

Activities of some enzymes, enzyme inhibitors and antinutritional factors from the seeds of sponge gourd (Luffa aegyptiaca M.)

Activities of some of the enzymes; enzyme inhibitors and antinutritional factors of the seeds ofLuffa aegyptiaca were investigated. Enzymes were extracted and their activities determined using standard procedures. The activities of trypsin and amylase inhibitors were also determined using standard procedures. While antinutritional factors were detemined using the AOAC method as well as standard procedures. The...

Author(s): Gloria N. Elemo, Babajide O. Elemo and Ochuko L. Erukainure

  • Article Number: A4AE6CF12216

March 2011

Extraction and application of dye extracted from eriophyid leaf galls of Quercus leucotrichophora-A Himalayan bluejack oak

Oaks (Quercus sp.) are susceptible to a wide diversity of gall-forming insects. When the insect pupates and leaves, the gall can be used as a dyestuff. Kalamkari unit’s method (India) was followed for the extraction of dye. A dark brown dye was obtained from the leaf gall of Quercus leucotrichophora caused by Eriophyes sp. Three different types of cloths and three different types of yarns...

Author(s): Preeti Mishra and Vidya Patni

  • Article Number: C36A5B612222

March 2011

Characterization of the polysaccharide material that is isolated from the fruit of Cordia abyssinica

Treatment of aqueous extracts of the fruit of Cordia abyssinica, containing 0.25% sodium chloride with three volumes of ethanol produced an acidic polysaccharide with a 2% yield, on a fresh weight basis. Upon precipitation of the polysaccharide with acid, the yield of the polysaccharide decreased to 1.2% and the polymer showed some level of degradation. With protein content between 2.6 and 4.6% for the acid and...

Author(s): Benhura, M. A. N. and Chidewe, C.

  • Article Number: 755380212226

March 2011

Trace metals and oxidative metabolic changes in malignant prostate cancer patients

Metabolic derangement commonly associated with prostate cancer (PCa) has not been well researched in Nigerian malignant PCa patients yet. The present study was designed to assess the levels of selected trace metals (Zn, Cu, Cr, Fe, Co, and Se) and markers of oxidative stress,such as total plasma peroxide (TPP), total antioxidant potential (TAP), oxidative stress index (OSI) and free malondialdehyde...

Author(s): Akiibinu M. O., Ogundahunsi A. O., Kareem OI, Adesiyan A. A., Idonije B. O. and Adeniyi F. A. A.

  • Article Number: 5899C5112233

March 2011

Molecular and biochemical novel cancer biomarker for early detection of breast cancer

Publisher's Retraction

Author(s): Patil V. W., Rajeev S. and Patil A. V.

  • Article Number: CCB974E41268

February 2011

Polymerization of human sickle cell haemoglobin (HbS) in the presence of three antimalarial drugs

To study the capacity of antimalarial drugs to interfere with polymerization of sickle cell haemoglobin (HbS), in vitro polymerization was induced by incubating erythrocyte haemolysate of HbS with 2 g% sodium metabisulphite (Na2S2O5) in the presence of 2 mg% concentration of three (3) separate antimalarial drugs (CoartemTM, Quinine, and Chloroquine phosphate). Spectrophotometric method was used to ascertain...

Author(s): Chikezie P. C., Chikezie C. M. and Amaragbulem P. I.

  • Article Number: 5A7B9DE12145

February 2011

Purification and biochemical characterization of a xylanase purified from a crude enzyme extract for the determination of active site residues

This paper describes the purification of a thermostable xylanase (Xyn) from a commercially available xylanase stock (Buzyme 2511 from Buckman Laboratories International, South Africa).The purified enzyme showed a Mr. of 66 kDa, and presented a dimeric form with two similar molecular weight chains of 33 kDa. The optimum pH and temperature were found to be 6.0 and 50°C, respectively. The enzyme was active over a broad...

Author(s): Sacha Tabosa-Vaz, Ajit Kumar, Kugen Permaul and Suren Singh

  • Article Number: 4162CCD12166

February 2011

Iodine and inorganic mineral contents of some vegetables, spices and grains consumed in Southeastern Nigeria

Selected vegetables, spices and grains which serve as staples in Southeastern Nigeria were analyzed for their iodine and some essential minerals. Our results showed that the mean iodine concentration of the vegetables were of 94.61 ± 15.28 µg/100 g, with Uha leaves (Pterocarpusspp.) having the highest with 117.66 ± 3.00 µg/100 g iodine, cowpea (Vigna...

Author(s): Ujowundu C. O., Kalu F. N., Nwosunjoku E. C., Nwaoguikpe R. N., Okechukwu R. I. and Igwe K. O.

  • Article Number: 35227FD12176

February 2011

Iodine nutrition for pregnant women in three major villages of Ohafia local government area in Abia State of Nigeria

Median urinary iodine concentrations of three hundred pregnant women in three major villages in Ohafia Local Government Area of Abia State, South-Eastern Nigeria were determined. This is to help determined whether iodine nutrition was sufficient or not in pregnant women. The analysis of iodine in urine was by spectrophotometric reaction in which specimens were manually digested with ammonium persulfate. The median...

Author(s): Igwe K. O., Ukoha A. I., NwaChukwu N. and Ujowundu C. O.

  • Article Number: 48F0D7612193

January 2011

In vitro biodegradation of keratin by dermatophytes and some soil keratinophiles

The present investigation was aimed to evaluate the in vitro biodegradation of keratin by clinical isolates of dermatophytes and soil fungi. Ten fungal species, out of which, six (Chrysosporiumindicum, Trichophyton mentagrophytes, Scopulariopsis sp., Aspergillus terreus, Microsporumgypseum and Fusarium oxysporum) were isolated from soil and four clinical...

Author(s): Mukesh Sharma, Meenakshi Sharma and Vijay Mohan Rao

  • Article Number: D687BEB12066

January 2011

ACTN3 and TNF gene polymorphism association with C-reactive protein, uric acid, lactate and physical characteristics in young African cricket players

The genotyping of α-actinin-3 (ACTN3) and tumor necrosis factor-α (TNF) genes of 31 Zulu South African males aged 20 to 27 years (14 cricketers and 17 controls) was performed by PCR amplification followed by restriction digestion. After ANOVA the associations with C-reactive protein (CRP), uric acid (UA), lactate (LA) and physical characteristics were examined using Chi2maximum likelihood test and...

Author(s): T. Djarova, G. Watson, A. Basson, J. Grace, J. Cloete and A. Ramakoaba

  • Article Number: 728E59512107

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