Microbial genes associated with methane metabolism in smallholder dairy cattle
June 2025
Methane gas is known for its negative environmental impact. To reduce negative methane gas released from dairy cattle, genes responsible for its production should be known and identified. A study with an aim of identifying the microbial genes responsible for methane gas release was undertaken. Samples of cattle’s stool on-farms (Lushoto and Rungwe districts of Tanzania) and a station-based (Kanyariri of Kenya)...
Production of amylase by Aspergillus species isolated from potato peels and soil in Lagos, Nigeria
June 2025
Thirteen fungal isolates were obtained from potato peels and soil in Lagos State Nigeria and were tested for amylase production by starch hydrolysis test. Production of amylase was indicated by zone of clearance around the microbial growth. On the basis of the clearance, five out of the thirteen isolates with zone of clearance of diameter above 20mm were characterized, identified and selected for further studies on...
Exploring the antimicrobial potential of marine seaweeds: A comprehensive review of bioactive compounds and their applications
June 2025
The global rise in antimicrobial resistance has heightened the quest for new and efficient antimicrobial agents. Marine seaweed is a rich source of several amino acids, fatty acids, and dietary fibers, as well as polysaccharides, polyphenols, pigments, and other active substances, which play crucial roles in various biological processes such as antioxidant activity, immunoregulation, and anti-inflammatory response....
Antibacterial activity and host range of phage filtrates from soil and banana plant pseudostems targeting Xanthomonas vasicola pv. musacearum
June 2025
The phytopathogen Xanthomonas vasicola pv. musacearum causes Banana Xanthomonas Wilt (BXW), affecting banana yield and productivity. This threatens food security and livelihoods of populations reliant on bananas. Various agricultural practices aim to manage BXW, yet the disease persists, reducing banana yield and productivity. Bacteriophages are natural enemies of bacteria and offer a potential biocontrol solution. This...
Characterization and pathological studies on Curvularia eragrostidis, the causal agent of Curvularia leaf spot disease of white yam in Ghana
May 2025
Yam leaf spotting diseases are important diseases of yam in the tropics. Mitigation strategies for these diseases are hindered by concerns regarding the variability of the causal agents. This needs to be studied for sustainable management strategies to be developed and employed in seed yam production. In this study, the identities, pathogenicity, and virulence of Curvularia isolates associated with Curvularia leaf spot...
Assessment of microbial contamination in home-produced wagashi, a traditional cheese from Kozah municipalities in Togo
May 2025
The traditional cheese, wagashi, widely consumed in Togo, was evaluated for its microbiological quality to assess the level of contamination and associated health risks. A total of 24 samples, randomly collected from various local producers in the municipalities of Kozah 1, 2, 3, and 4, were submitted to microbiological analyses in triplicate to ensure accuracy and reliability, using standard microbiological methods....
Antimicrobial resistance of clinical and environmental klebsiella pneumoniae isolates in selected areas of Lusaka, Zambia
May 2025
Klebsiella pneumoniae causes community / nosocomial infections in humans. In animals it causes mastitis, resulting in low milk productions and deaths. There is emerging, increase, progressive spread of AMR strains in healthcare settings. This poses public health risk and drain on national financial resources. A cross-sectional study was conducted from July 2019 to January 2021 to evaluate clinical /...
Assessment of antibiotic abuse among patients visiting out-patient department in a teaching hospital in Ghana
May 2025
Antibiotic abuse and the emergency of its resistance is a public health concern globally. This has resulted in protracted hospitalization, increased medical costs, and mortality. Self-medication, poor knowledge of antibiotics use and lack of enforcement of regulatory policies on their sale have been reported as a major conduit necessitating abuse by patients prior to visiting healthcare facilities in many developing...
Assessing the effects of two fermentation methods on the physico-chemical, microbiological and sensory properties of “placali” prepared from three improved cassava varieties
April 2025
Placali is a paste made by fermenting a mixture of cassava chips after they have been ground and pressed. However, the organoleptic quality of placali differs depending on the producer. This fluctuation in quality is not only due to the variety of cassava but also, and above all, because of the type of fermentation process used. The aim of this study is to evaluate the impact of two types of ferments on the quality of...
Broad-spectrum antibacterial activity and membrane integrity disruption by Zingiber officinale extracts in Gram-positive and Gram-negative bacteria
April 2025
Zingiber officinale (ginger) has been extensively studied for its antibacterial properties. This study evaluated the antibacterial activity of acetone and water extracts of Z. officinale against Gram-positive and Gram-negative bacteria using deep-well diffusion, minimum inhibitory concentration (MIC), potassium ion efflux, and Bradford protein assays. Bacterial strains, including Gram-positive Enterococcus faecalis...
Overview of laboratory-confirmed measles cases in Ivory Coast between 2003 and 2022
April 2025
Virological surveillance of measles virus (MeV) is a key component of measles elimination program. The goals of this study were to describe the epidemiology of measles infection and to evaluate MeV genotype strains circulating in Cote d’Ivoire. ELISA tests and viral isolation were performed on biological samples from suspected measles patients. Viral RNA was also extracted either directly from clinical...
Heavy metals in effluents from Bodija abattoir, and their effects on organisms in Oshunkaye Stream, Ibadan, Nigeria
April 2025
The size of grain and analysis of heavy metals in sediments, waste, and benthic organisms from the Oshunkaye stream due to heavy effluent from the Bodija abattoir are investigated with samples taken from six different sampling points. The mean size analysis in all six sampling points is sand, very rough grain, and rough grain particle sizes between Phi -2.0 to 1.0. The skew values of the sediment size in the samples...
In vitro antagonistic potential of Trichoderma strains against Ralstonia solanacearum
March 2025
Potato (Solanum tuberosum) seed production is a critical element of the potato industry in Zimbabwe. Production in the potato industry is constrained by poor seed quality caused by the bacterial wilt pathogen Ralstonia solanacearum. The pathogen is known to be highly destructive in potatoes. R. solanacearum produces several virulence factors which invade the plant’s natural defense mechanism. Trichoderma species...
Molecular investigation of pathogenic Leptospira in bat species in Abidjan, Côte d’Ivoire
March 2025
Leptospirosis is a bacterial zoonosis caused by pathogenic Leptospira strains, primarily affecting tropical and subtropical regions. In Côte d'Ivoire, previous studies on rodents and cattle have failed to establish a link between human cases and animal reservoirs, suggesting an unidentified source of infection. Given that bats are known to host various human pathogens, this study aimed to detect pathogenic...
Characteristics of antibiotic resistance of Escherichia coli strains in people suffering from gastroenteritis in the Republic of Guinea
February 2025
This study aims to assess the spectrum of antibiotic resistance and to determine the antibiotic resistance genes of Escherichia coli strains isolated from patients suffering from diarrhea. It was carried out from 2019 to 2022, alternating between in-patients at Kindia Regional Hospital. A coproculture was performed, strains were identified using the API 20E kit and antibiograms were performed using the Kirby bauer...
Isolation, biochemical and molecular characterizations of riboflavin-producing lactic acid bacteria from Nigerian fermented food products
January 2025
The purpose of this research was to explore lactic acid bacteria (LAB) from Nigerian fermented foods that can be used to produce riboflavin. In this study, LAB were isolated from “wara”, “nono”, and “ogi”. Total number of colonies of LAB in triplicates ranged from 3.9 × 106 to 4.2 × 106 CFU ml-1. A total of 321 LAB were isolated. Of these LAB isolates, 129 (40%) belong to...
Detection of carbapenemase-producing strains of Gram-negative bacilli in University Hospital of Angre in 2021
November 2024
The spread of carbapenemase-producing Gram-negative bacilli (GNB) poses a significant threat to public health. In Africa, data on carbapenem-resistant bacteria are poorly defined. This study aimed to determine the prevalence of carbapenemase genes among GNB isolated from patient samples at Angre University Hospital. A cross-sectional study was conducted in 2021 at the medical biology department, where...
Practice of prophylactic antibiotic use in elective orthopedic procedures at a national teaching and referral hospital in Kenya
November 2024
According to the Centers for Disease Control and Prevention (CDC) recommendations, antibiotic prophylaxis should only be used when necessary. The judicious use of prophylactic antibiotics, following evidence-based guidelines, leads to optimal outcomes, including reduced infection rates and the prevention of antimicrobial resistance. This study examines the practice of prophylactic antibiotic use in elective orthopedic...
Detection of norovirus in fresh fruits, vegetables, wastewater and manure from irrigated farms in Ouagadougou, Burkina Faso
October 2024
Noroviruses are the primary cause of foodborne infections associated with fresh products. The aim of the present study was to assess agricultural practices and the presence of noroviruses in fruits and vegetables, manure, and irrigation wastewater sampled from the urban and peri-urban irrigated plots of Ouagadougou. A face-to-face survey was conducted using a questionnaire on market garden production practices. A total...
Anti-biofilm formation effects of aromatic and medicinal plant extracts on Xanthomonas axonopodis pv. Phaseoli
September 2024
Common bacterial blight (CBB) caused by Xanthomonas axonopodis pv. phaseoli (Xap) have been recorded as one of the most important seed-borne destructive diseases on beans. Chemical compounds which are incessantly used on beans to manage CBB disease cause serious damage to natural ecosystems and sometimes they show frequent failure for controlling CBB. These consequences are attributed to the use of by-products from...
Recent developments and future prospects of extremozymes in detergent applications
August 2024
Extremozymes are stable and active under harsh conditions, which is useful in industrial processes where mesophilic enzymes are destroyed. The applications of such enzymes are found in detergent industries due to their special characteristics. Microorganisms that survive in hot springs, cold regions, soda lakes, and even contaminated environments are explored for extremozyme production. The addition of these enzymes to...
Epidemiology, prevalence and seroprevalence of COVID-19 among vulnerable people in Thies region, Senegal
June 2024
Cases of pneumonia linked to a new Coronavirus have been noted in Wuhan city, Hubei province in China. This epidemic caused by the new Coronavirus called COVID-19 by the WHO, quickly became a global pandemic. This study is to assess the epidemiological situation as well as the prevalence and seroprevalence of COVID-19 in 213 vulnerable patients in the Thies region. This is a prospective study, targeting vulnerable...
Antimicrobial properties and antioxidant activities of pigeon pea seed protein hydrolysates
June 2024
In recent times, the biological activities of enzymatic digests of plant and animal proteins have been investigated and have been shown to exhibit multidirectional effects against microorganisms involved in the pathophysiology of a number of diseases. The present study evaluated the antimicrobial and antioxidant effects of pigeon pea protein hydrolysates. Proteins were hydrolyzed using the enzymes pepsin, trypsin and...
Phytochemical study and evaluation of the antimicrobial activities of three plants in the treatment of typhoid fever in Togo
June 2024
Typhoid fever, an infectious disease impacting millions worldwide, has led to the utilization of a traditional medicine recipe in Togo consisting of Carica papaya, Cocos nucifera, and Persea americana. This study aimed to assess the phytochemical properties and antimicrobial activities of the traditional medicine recipe against typhoid fever. Qualitative tests, employing characterization reactions, were employed to...
Resistance profile of bacteria isolated from Broncho alveolar lavage fluid (BALF) of patients with severe acute lung disease at the University Hospital Center of Cocody in Abidjan, Côte d’Ivoire
May 2024
This study aimed to characterize the microorganisms responsible for severe acute respiratory infections in adult patients hospitalized at the University Hospital Center of Cocody in Abidjan. The biological material consisted of bronchoalveolar lavage fluid (BALF). Isolation and identification of bacterial strains, along with susceptibility testing, were performed using standard microbiological techniques. The bacteria...
A metagenomic analysis reveals changes in the bacterial community structure due to infection by the rice yellow mottle virus
May 2024
This research aimed to explore the pathobiome to enhance the understanding of the roles played by disease-associated microbes. A comparative analysis of microbiomes in rice samples was conducted to shed light on the potential impact of the rice yellow mottle virus on microbiome composition. Forty samples were collected from rice fields of the Office du Niger in Mali. Bacterial DNA was extracted at the LaboREM-Biotech...
Resistance profile of urine isolate enterobacteral strains at Donka University teaching hospital in Conakry, Guinea
April 2024
The objective of this study was to describe the resistance profile of enterobacterals isolated from urine samples at the laboratory of Donka National Hospital. Urine samples were collected from both outpatients and hospitalized patients. Cultures were performed using standard techniques, strains were identified using the API 20E kit, and antibiotic susceptibility testing was carried out using the ATB™ UR EU (08)...
Exploring the antimicrobial modulatory potential of the sap from oil palm tree
April 2024
The sap from the oil palms (Elaeis guineensis), harbours complex microbiota and provides a rich source of therapeutic metabolites. This study investigated the antibacterial modulatory activity of sap from Elaeis guineensis on selected bacteria. To test how well the sap from the oil palm tree affects bacteria, the minimum inhibitory concentration (MIC) of some common antibiotics was found by mixing the sap from the oil...
Characterization of novel potential probiotic strains of lactic acid bacteria from rat faeces
March 2024
This study aimed to isolate and characterise novel probiotic strains from the faeces of healthy albino Wistar rats. Lactic acid bacteria were isolated on MRS agar, and their probiotic properties were assessed through in vitro tests, including tolerance to simulated gastrointestinal juices, auto-aggregation assays, and antimicrobial activity. The antibiotic susceptibility and haemolysis tests were performed to assess the...
Mycoplasma galliscepticum: An overview
March 2024
The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny...
Antibiotic resistance profile of Escherichia coli isolates among patients suspected of urinary tract infections at the Souro Sanou University Hospital Centre of Bobo-Dioulasso, Burkina Faso
February 2024
This study aims to describe the antibiotics resistance (antimicrobial resistance [AMR]) profile of Escherichia coli isolates among patients suspected of urinary tract infections (UTIs) at the Souro Sanou University Hospital Center (SSHUC) of Bobo-Dioulasso in Burkina Faso. A descriptive cross-sectional study was conducted from June 1 to August 31, 2021 at SSUHC. An exhaustive sampling was performed and the urine samples...
Microbiological evaluation and possible origins of the microbial contamination of vegetables in Ouagadougou (Burkina Faso)
February 2024
This study aimed to contribute to the health safety of widely consumed vegetables in Ouagadougou by investigating the potential contamination of onion, tomato, cucumber and lettuce with pathogenic microorganisms. A survey was conducted, involving 102 producers in fields, 102 vendors in markets, and 205 consumers. The most commonly consumed vegetables were identified as onion, tomato, cucumber and lettuce. A total of 264...
Microbiological quality and safety of poultry processed in Africa: A review
January 2024
Poultry is the second most consumed meat in the world. In Africa, chicken production and processing are practiced both formally and informally, with smallholders constituting the majority in this sector. Informal practices are vulnerable to the production and processing of chicken, which is easily contaminated by pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella, and Campylobacter. The growth of the...
Antibacterial activity and phytochemical investigation of leaf and root extracts of Aloe gilbertii Reynolds
January 2024
The objective of the study was to test the antibacterial activities of crude extracts of roots and leaves of Aloe gilbertii Reynolds against clinical pathogens. The crude extracts were prepared via maceration technique employing n-hexane, acetone, chloroform, dichloromethane:methanol (50:50% V/V) and methanol solvent system. The phytochemical screening tests of the dichloromethane:methanol (50:50% V/V) root extract of...
Antibiotic sensitivity patterns of nosocomial infections bacterial isolates in National Hospital in Niamey, Niger
January 2024
Nosocomial infections are recognized as a global public health issue, with causative bacteria often exhibiting high levels of antibiotic resistance. However, there is a paucity of data on multidrug-resistant bacteria in nosocomial infections in Niger. This study aimed to determine the antibiotic sensitivity of bacteria isolated from specimens of patients suffering from nosocomial infections. Samples collected from...
Acute bacterial meningitis ecosystem in Cote D'ivoire from 2012 to 2020
December 2023
Bacterial meningitis remains a health concern in meningitis belt countries. The objective of this study was to ascertain the bacterial profiles of meningitis in Côte d'Ivoire from 2012 to 2020. The study, conducted retrospectively at the National Center of Reference of Meningitis of Cote d’ivoire, analyzed 5046 cerebrospinal fluid (CSF) samples from January 2012 to December 2020. Epidemiological data...
Prevalence, antibiotic resistance of Staphylococcus aureus isolated from layer farms (eggs, droppings/litter) in the outskirts of the city of Ouagadougou, Burkina Faso
December 2023
Eggs are susceptible to microbiological contamination by various pathogens on farms, particularly those belonging to the genus Staphylococcus, which are zoonotic bacteria capable of contaminating humans, animals, and/or birds, posing a significant public health concern. In Burkina Faso, limited data exists on the prevalence of Staphylococcus aureus, prompting this study to investigate the prevalence and antibiotic...
The assessment of contamination level of multidrug resistant bacteria and antibiotic residue in chicken manure
December 2023
This study aimed to assess the contamination level of chicken litter by antibiotic residues and multidrug resistant (MDR) bacteria. A total of 28 chicken litter samples were aseptically collected in Abidjan poultry farms in December 2021. The bacteria including Salmonella, Escherichia coli and Staphyloccocus aureus were isolated on specific culture media and were identified using biochemical tests. Antibiotics...
Molecular identification of biofilm-producing Bacillus species and yeasts isolated from food sources and their interaction with Lysinibacillus louembei strain
December 2023
The microorganisms contained in fermented foods are known to harbor metabolic products, possibly improving human and animal health. However, despite several studies on the functional effects of fermented foods, isolation and identification of the effective Bacillus species strains are still in progress. The objective of this study was to molecularly identify biofilm-producing Bacillus spp. (BPB) and yeasts from...
Denaturing gradient gel electrophoresis (DGGE): An alternative culture independent method for bacterial screening in bovine milk sample
November 2023
Bovine mastitis is a disease that has a great impact on Brazilian livestock production. Some bacteria described as mastitis-causing agents are not easily cultivable in conventional media, making their diagnosis difficult. The aim of the present study was to detect bacteria present in milk from mastitic and non-mastitic quarters of dairy cattle in Rio de Janeiro-Brazil, using a culture-independent and culture-dependent...
Evaluation of the impact of the efflux pump modulation process on the anti-adhesion and antibiofilm activities of analogues of natural marine compounds in a marine bacterium
November 2023
One strategy for enhancing the effect of natural marine antifouling compounds has been to combine them with coumarin-type molecules (VL2, VL17 and VL19). These molecules are known to have an inhibitory effect on the efflux pumps (EP) of a large number of Gram-negative bacteria. To this end, they have been used as adjuvants to natural marine compounds (OB1, AS194 and AS162) to enhance their antibiofilm activity. On the...
Effectiveness of temocillin on extended-spectrum beta-lactamase producing Escherichia coli isolates from patients attending the Niamey General Reference Hospital
November 2023
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli urinary tract infections represent a major global health problem, constituting a public health emergency with high patient morbidity and mortality. Temocillin serves as an alternative antibiotic for treating these infections and conserving carbapenems. A total of 209 isolates of uropathogenic Escherichia coli were collected in the Microbiology Laboratory...
Effect of sanitizers combined with ultrasound on adhesion of microorganisms isolated from fish from the Amazon region
October 2023
Attachment of undesirable microorganisms to surfaces that contact food is a source of concern, since it can result in product contamination leading to serious economic and health problems. Bacteria aggregated to form biofilms are more resistant to environmental stress than planktonic cells. The objective of this paper was to evaluate the bactericidal effect of sodium hypochlorite and peracetic acid associated with...
Growth, root colonization and yield attribute responses of five groundnut (Arachis hypogaea L.) varieties toward arbuscular mycorrhizal fungal inoculation in a Senegalese agricultural soil
October 2023
The aim of this study was to isolate the most specific and effective arbuscular mycorrhizal fungi (AMF) for groundnut and to determine the degree of variability in the response of groundnut varieties to inoculation. The seeds of five varieties: 55-437, Fleur 11, Sunu Gaal, Amoul Morom, and Essamaay were inoculated individually with five AMF (Funneliformis mosseae, Rhizophagus aggregatus and Rhizophagus fasciculatus, the...
Molecular detection of pathogenic Leptospira from cattle in peri-urban areas of Addis Ababa, Ethiopia
October 2023
Leptospirosis is a bacterial, zoonotic disease caused by pathogenic Leptospira. Rodents are known to carry pathogenic Leptospira, but livestock are also important hosts. The disease is economically important in cattle, causing abortion, decreased fertility and decline in milk yield. Pathogenic Leptospira are shed in cattle urine and can survive in the environment. Only a few studies have been performed in Ethiopia to...
Effect of methylprednisolone on the morphology of acute lymphoblastic leukemia cell line in vitro model by Atomic Force Microscopy
September 2023
Despite the recent knowledge of the molecular mechanisms of corticosteroid drugs in lymphoid leukemia, information about the nanostructured surface morphology of the process of cell death is limited. Therefore, the main objective of this work is to analyze the effect of methylprednisolone on the morphology of B lymphoblast CCRF-SB cell line by Atomic Force Microscopy (AFM). Morphological parameters such as height, cell...
Density and diversity of arbuscular mycorrhizal fungi in Anacardium occidentale L. plantations in Senegal
September 2023
Cashew nut cultivation plays an important socio-economic role in Senegal. However, it suffers from several problems, including declining soil fertility which leads to low productivity. To overcome these constraints, the use of arbuscular mycorrhizal fungi (AMF) could be a sustainable alternative. However, the positive effects of AMFs may depend on their infectious potential, density, and diversity. So far, little...
Screening groundnut rhizobia for multiple plant growth promoting activities in Ethiopia
September 2023
Rhizobia could exhibit plant growth-promoting mechanisms besides nitrogen fixation. In search of efficient rhizobia with multiple PGP traits, 72 groundnut nodulating rhizobia isolates were screened in vitro for their plant growth promoting traits like phosphate solubilization, production of indole acetic acid, ammonia, hydrogen cyanide, production of different hydrolytic enzymes and antifungal activity. About 72% of the...
Morphological, pathogenic and molecular characterization of fungal species associated with mango fruits in Mexico
August 2023
The characterisation of the causal agents of postharvest diseases, as well as their pathogenicity in different mango cultivars of economic importance in Mexico was evaluated. In total, 37 fungi were isolated from diseased mango (Mangifera indica L.) of the cvs Ataulfo, Hayden, Manila, Peach, Haden and creole. For the morphological characterization, the isolates were seeded in potato dextrose agar media and described by...
Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts
August 2023
The antimycotic effect of Casia alata (Linn.) commonly used for the treatment of skin diseases by local people in Nigeria was evaluated against dermatophytes (Trichophyton tonsurans, Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Epidermophyton floccosum). Ten-fold serially diluted extract concentrations were subjected to phytochemical screening and the...
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