African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5059

AJMR Articles

Assessment of the hand hygiene status of internally displaced persons in Jos and environs, Nigeria

November 2020

Internally displaced persons (IDPs) are often predisposed to infectious diseases because of the temporary nature of their abode which usually does not have adequate water and hygiene facilities. A hundred (100) samples of hand-washed water taken under aseptic conditions from the hands of the IDPs prior to the emergence of COVID-19 were analyzed using standard bacteriological procedures to determine the total plate and...

Author(s): John O. Egbere, Collins E. Odu, Anayochukwu C. Ngene, Margaret M. A. Danladi, Chinedu G. Ohaegbu and Philomena D. Ayika

Antibacterial properties of traditional Sudanese medicinal materials against selected enteric bacterial strains

October 2020

Folklore medicine in Sudan used medicinal materials to treat inestinal infections caused by bacterial infections or contamination of food. Methanolic and aqueous extracts of different parts of Acacia nilotica (L.) Delile gum, Haplophyllum tuberculatum Juss.aerial parts, Hydnora abyssinica A. Br.fruits, Nigella sativa L seeds, Rhynchosia minima (L.) DC. roots,and Usnea molliuscula lichen were tested for antibacterial...

Author(s): Sana Eltayeb Mahjoub

Prevalence and drug-resistance profile of plasmid-borne extended spectrum beta-lactamase (ESBLs) resistance genes in multidrug resistant Escherichia coli from HIV-1 positive individuals in Jos, Nigeria

October 2020

Production of extended-spectrum beta-lactamases (ESBLs) can lead to treatment failures when the antibiotics are used. This study detected ESBLs genes on Multidrug Resistant Escherichia coli from HIV-infected individuals attending some hospitals in Jos. Eighty (80) isolates of multidrug resistant (MDR) E. coli were screened for plasmids. ESBLs genes including bla-CTX, bla-TEM and bla-SHV were detected on the plasmids...

Author(s): Murna Ahmed Ali, Ocheme Julius Okojokwu, Ujeh Anthony Augustine, Chad Achenbach, Joseph AjeAnejo-Okopi, Patricia MankoLar, Godwin Imade and Atiene Solomon Sagay

Resistance of Candida albicans to antifungal drugs in Abidjan (Cote d’Ivoire)

October 2020

This study aimed to evaluate the resistance levels of strains of Candida albicans to the antifungals commonly used in Abidjan, Cote d’Ivoire. This is a prospective study that was carried out from July to October 2017 at the mycology laboratory of the Institut Pasteur of Cote d’Ivoire. 105 Candida isolates, obtained from swabs taken from patients receiving out-patient treatment, were seeded on chromogenic...

Author(s): C. G. Kouadio-Yapo, N. A. D. Aka, A. V. Bonouman-Ira, G. S. P. Dou, K. H. G. Sonan, K. D. Zika, K. D. Adoubryn and M. Dosso

Arbuscular mycorrhizal fungi (AMF) promote the growth of the pioneer dune plant of coastal areas

October 2020

The establishment of economic enterprises and the demand for coastal areas for leisure purposes exert great pressure on the dunes, stripping them of vegetation and causing the aggravation of coastal erosion processes. The use of arbuscular mycorrhizal fungi (AMF) is a viable alternative to restore dunes, giving their ability to improve soil conditions and plant growth under unfavorable conditions. The aim of this study...

Author(s): Rosmim António Tivane, Íris Victorino, Sónia Ventura Guilundo, Rui Oliveira, Célia Marília Martins and Orlando António Quilambo  

Prevalence and Antibiotic resistance profile of Avian Pathogenic Escherichia coli (APEC) strains isolated from poultry feeds in Abidjan District, Côte d'Ivoire

October 2020

The aim of this study was to isolate avian pathogenic Escherichia coli (APEC) strains from poultry feeds for assessing their susceptibility patterns to antibiotic agents. From November 2018 to March 2019, one hundred eighteen poultry feeds samples were collected in farms of Abidjan District and E. coli strains were isolated on TBX (Tryptone Bile Glucuronic) agar medium, followed by biochemical identification and APEC...

Author(s): Christelle Suzanne Djoman, Eric Essoh Akpa, Bernadette Gblossi Goualié, Lamine Samagassi and Delphine Yevi N’Guessan

Isolation and morphological characterization of endophytic fungi isolated from mangrove plants along the Kenyan coastline

October 2020

Microorganisms in marine ecosystems are exposed to harsh conditions, thus such systems are of interest in bioprospecting for useful secondary metabolites. The aim of the study was to isolate and identify the fungal endophytes that colonize mangroves. The fungal endophytes were isolated from the leaves, roots, and branches of different mangrove plants (Bruguiera gymnorrhiza, Heritiera littoralis, Xylocarpus...

Author(s): Teresia Nyambura Wacira, , Huxley Mae Makonde, Carren Moraa Bosire, Suleiman Said Mzee, Samuel Mwakisha Mwamburi and Cromwell Mwiti Kibiti  

Coronavirus disease 2019 (COVID-19) pandemic: What is the level of knowledge, attitude, and practice in Kandahar, Afghanistan?

September 2020

There is a rising concern for the rapid increase of COVID-19 confirmed cases in Kandahar province. From zero reported cases until 17th March 2020, then Kandahar saw a sudden rise in the cases by 16th May 2020. Decreased literacy rates, poor health education, lack of facilities, inconsistent government policies, and defying coronavirus safety advisory by the public have resulted in the rapid spread of COVID-19. The...

Author(s): Asmatullah Usmani, Abdul Qadeer Baseer, Bilal Ahmad Rahimi, Ajmal Jahid, Parwiz Niazi, Abdul Wahid Monib, and Wais Mohammad Lali  

Bacterial community composition and diversity along the southern coastlines of the Atlantic Ocean in Cape Town, South Africa

September 2020

The spatial distribution and diversity within bacterioplankton assemblages in four coastal sites along the southern points of the Atlantic Ocean were examined using the Illumina high-throughput that targets 16S rRNA genes to examine indigenous bacterial assemblages in the littoral zones along the coast of the ocean. Results of the study showed very similar bacterial representation between the coastal sites with majority...

Author(s): Ola A. Olapade  

Biochemical and molecular characterization of yeasts isolated from Nigerian traditional fermented food products

September 2020

Yeasts are very important in food production by affecting the quality and safety of different food products. Yeasts are commonly used in the production of beer, wine and bread; but many yeast species have been isolated from different African fermented foods. The present study aims to determine the biodiversity of yeasts isolated from selected Nigerian fermented foods. A combination of phenotypic and molecular tests were...

Author(s): Adesokan Isaac Ayanniran, Sanni Abiodun and Marc-Andre Lachance  

Quality assessment of bacterial load present in drinking water in Woreta town, Ethiopia

September 2020

Access to adequate and safe water is a universal human need. Lack of safe and adequate water supply causes health risk  and the situation is serious in developing countries  The aim of this study was to determine the bacterial load, physicochemical quality of drinking water in wells, reservoir, taps and household storage containers water samples  and to investigate the hygiene and sanitation practices of...

Author(s): Kindu Alem  

Differential traits of rhizobia associated to root-nodules of gum acacia (Senegalia senegal), shittah tree (Vachellia seyal), pigeon pea (Cajanus cajan L) and cowpea (Vigna unguiculata)

September 2020

In the present study, rhizobia partners of gum acacia (Senegalia senegal L.), shittah tree (Vachellia seyal), pigeon pea (Cajanus cajan L.) and cowpea (Vigna unguiculata L.) were characterized for their specific traits to each of the three host plants. In total 12 soil samples were collected and used to trap root-nodule rhizobia. Averagely, 24 rhizobia strains were isolated, of which 3, 4, 6 and 11 were respectively...

Author(s): Justin Ganava, Doloum Gomoung, Laurette Ngo Nkot, Steve Takoukam Toukam and Albert Ngakou  

Assessment of microbiological quality and drug resistance patterns of raw vegetables irrigated with Hasassa River, West Arsi Zone, Oromia Region, Ethiopia

September 2020

Irrigation scheme is among the major transmission modes of enteric human pathogens. Irrigation of vegetables with polluted water and untreated wastewater is a common practice worldwide. This practice is common in urban areas of low-income countries including Ethiopia, where there is increasing demands for fresh vegetables with poor wastewater treatment. The aim of the present study was to assess the microbial quality...

Author(s): Zerihun Belay, Edao Gelgelu, and Bayissa Chala  

Leishmanicidal, cytotoxic, antimicrobial and enzymatic activities of Diaporthe species, a mangrove-isolated endophytic fungus

September 2020

This study evaluated the biotechnological potential of the endophytic fungus Diaporthe species, which is isolated from Brazilian mangroves. In vitro studies have investigated the antimicrobial activity of Diaporthe spp. against human pathogens, such as Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, and Candida albicans, as well as phytopathogenic fungi, such as Colletotrichum species, Ceratocystis...

Author(s): Carla Cristina Moreira, Genoveva Lourdes Flores Luna, Bianca Soriano,Rafael Cavicchioli, Andréa Cristina Bogas, Cristina Paiva de Sousa, Fernanda Freitas Anibal, and Paulo Teixeira Lacava,  

Molecular identification of bacterial isolates from the rhizospheres of four mangrove species in Kenya

September 2020

Mangrove ecosystems provide a unique ecological niche for diverse microbial communities. This study aimed to identify bacterial isolates from the rhizospheres of four mangrove species (Sonneratia alba, Rhizophora mucronata, Ceriops tagal and Avicennia marina) using the 16S rRNA gene analysis approach. Rhizospheric sediment samples of the mangroves were collected from Mida creek and Gazi bay, Kenya, using standard...

Author(s): Edith M. Muwawa, Huxley M. Makonde, Joyce M. Jefwa, James H. P. Kahindi and Damase P.  Khasa  

Isolation and identification of microorganisms in selected cosmetic products tester

September 2020

Components of cosmetics supply a rich medium for the growth of microorganisms. Customers usually like to try cosmetics before buying them. Repetitive use of the eye and makeup tester by different customers may become a potential vector of microbial pathogens. The major goal of this study was to evaluate microbial contamination of the cosmetics that are accessed as an unsealed make-up tester in the selected makeup shops...

Author(s): Noor A. I., Rabih W. M., Alsaedi A. A., Al-Otaibi M. S., Alzein M. S., Alqireawi Z. M., Mobarki K. A., AlSharif R. A. and Alfaran H. S.  

Isolation, characterization and biotechnological potential of tropical culturable rhizospheric fungi from four mangrove species in Kenya

September 2020

Fungi play important roles, as both decomposers and plant symbionts in mangrove ecosystems. Their ability to survive extreme environmental conditions makes them potential rich sources for recovery of novel bioactive compounds. In this study, biotechnological potential of fungi recovered from the rhizospheres of four mangrove species (Sonneratia alba, Rhizophora mucronata, Ceriops tagal and Avicennia marina) was...

Author(s): Edith M. Muwawa, Huxley M. Makonde, Chinedu C. Obieze, Joyce M. Jefwa, James H. P. Kahindi and Damase P. Khasa  

Evaluation of the microbiological quality of "mabokés" smothered fish and, proteolytic activity of bacteria of the genus Bacillus in Brazzaville, Congo

August 2020

In order to assess the health risks related to the consumption of smothered fish "mabokés" sold in the markets of Brazzaville, samples of smothered fish sold in three markets in Brazzaville were analyzed for their microbiological quality. The different samples were cultured for isolation on solid media using conventional microbiological methods. The bacteria isolated were identified on the basis of...

Author(s): Cyr Jonas Morabandza, Tarcisse Baloki Ngoulou, Valentin Dibangou, Saphia Jemylah Galekoua Ndjiwa Empilo, Armel Faly Soloka Mabika, Christian Ngo - Itsouhou, Rachel Moyen and Gabriel Ahombo  

Evaluation of micro-fungi associated with leaf spot of Allanblackia floribunda Oliv. in Southern Nigeria

August 2020

Fruit of Allanblackia floribunda is an active ingredient in the pharmaceutical industry. Fruit production in the species is hindered by incidence of pathogenic fungi leading to economic loss. This study was conducted to investigate micro-fungi species associated with leaf spot of A. floribunda. Field surveys were carried out in natural stands containing matured  A. floribunda trees  located at Owu Ikija, Ogun...

Author(s): Olusola O. Olasupo Adebola O. Adegeye and Samuel O. Olajuyigbe  

A comparison of antibacterial efficacy of some household detergents available in Makkah, Saudi Arabia, against 16 ATCC bacterial strains and clinical isolates

August 2020

The emergence and spread of multidrug-resistant microorganisms are threats to public health due to its related increasing rates of morbidity and mortality in the world. Routine disinfection practices do not eliminate many of these resistant species and may contribute to the development in their resistance. Therefore, the current study evaluates the effectiveness of some popular detergents labelled as antibacterial and...

Author(s): Hamdi M. Al-Said and Sami S. Ashgar

Antibiotic resistance and genotypic detection of extended spectrum beta-lactamase producing pathogens in catheter associated urinary tract infection at a teaching facility in Kumasi, Ghana

August 2020

This study determines the antibiotic resistance pattern of extended spectrum beta-lactamase (ESBL) producing pathogens responsible for catheter associated urinary tract infection (CAUTI) and the genes associated with the resistance. The study used 35 ESBL-producing pathogens isolated from urine and biofilm found in CAUTI from June 2018 to November 2018. Pathogens were confirmed phenotypically for ESBL production with...

Author(s): Alexander Bekoe, Roland Azorliade, Anthony Ablordey and Matthew Glover Addo

Water quality index (WQI) and bacteriological assessment of some selected hand-dug wells in the Adenta municipality, Ghana

August 2020

Water quality was assessed from 11 hand dug wells in the Adentan Municipality using Water Quality Index (WQI) and bacteriological load as indicators. The sampling was conducted during the months of July to September, 2019. A total of 33 samples were taken from 11 hand dug wells at monthly intervals. Weighted Average Water Quality Index (WAWQI) was used to compute parameters which include temperature, pH, conductivity,...

Author(s): Phillip Adu-Gyamfi, John Asiedu Larbi and Matthew Glover Addo

Metabolomics analysis of carotenoid production from Proteiniphilum acetatigenes PSB-W based on LC-MS

August 2020

Metabolomics based on LC-MS was used for the analysis of the fermentation of Proteiniphilum acetatigenes PSB-W treated by different culture time and different light intensity. 26 putative metabolites were detected in samples treated by different culture time. Most of the components were produced in the metabolic activity in order to participate in the whole life process of bacteria. Compared with samples treated with...

Author(s): Qingping Hu, Xiaoli Gao and Yan Wang  

Antibiotic susceptibility patterns of bacteria isolates from post-operative wound infections among patients attending Mama Lucy Kibaki Hospital, Kenya

August 2020

Surgical site infections account for high mortality, morbidity, and elevated costs of treatment for surgical patients. The study sought to determine the prevalence and antibiotic susceptibility patterns of bacterial isolates from postoperative wound infections among patients attending Mama Lucy Kibaki Hospital. A cross-sectional descriptive study was carried out between October 2018 and March 2019. It included patients...

Author(s): Johnstone Amulioto, Margaret W. Muturi, Scholastica Mathenge and Gideon M. Mutua  

Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin

August 2020

Momordica charantia and Morinda lucida are Benin’s pharmacopeia plants that are used traditionally for the treatment of infectious diseases. This study aims to investigate phytochemical profile and antimicrobial activity of both plants. The dried leaf powder is used for extraction with different solvents by ultrasonication (35 Hz) at room temperature for 2 h. TLC and the method based on coloring and precipitation...

Author(s): Michaelle Chokki ,, Cheikna Zongo, Durand Dah-Nouvlessounon, Mihaela Cudălbeanu, Pacôme Noumavo, Ioana Otilia Ghinea, Bianca Furdui, Aly Savadogo, Rodica Mihaela Dinica, Lamine Baba-Moussa and Farid Baba-Moussa

Effect of using treated wastewater on the bacteriological quality of raw cow's milk: A case of a farm in Northeastern Algeria

August 2020

This study aims to assess the impact of the use of treated wastewater (without chlorination) in farming and dairy cattle breeding. Milk samples were collected from a farm in northeastern Algeria. The treated wastewater from the treatment plant is used on this farm for different activities. The results obtained show that the average contamination of milks with total flora is 3.7.105 CFU /ml. Fecal coliforms are...

Author(s): Keltoum Chorfi,, Katia Bendjemana, Ammar Ayachi, Fatma Mahdi and Nour El Aimane Bouzidi  

Combination of genetic tools to discern Bacillus species isolated from hot springs in South Africa

August 2020

Using phylogenetic analysis of the 16S rRNA gene 43 Gram-positive, spore-forming bacteria of the phylum Firmicutes were isolated, cultured and identified from five hot water springs in South Africa. Thirty-nine isolates belonged to the family Bacillaceae, genus Bacillus (n = 31) and genus Anoxybacillus (n = 8), while four isolates belonged to the family Paenibacillaceae, genus Brevibacillus. The majority of isolates...

Author(s): Jocelyn Leonie Jardine and Eunice Ubomba-Jaswa,  

A Malian native Azospirillum sp. Az6-based biofertilizer improves growth and yield of both rice (Oryza sativa L.) and maize (Zea mays L.)

July 2020

The objective of this study was to improve rice and maize yields using native Azospirillum-based biofertilizer. To reach this objective, samples of rhizosphere soil, non-rhizosphere soil and roots of maize plants were collected from the particular locations of Samanko and Bamako of the south Mali. Thirty-three different colonies of bacteria were isolated from the different samples. Based on their better growth in...

Author(s): Ibrahima Mallé, Adounigna Kassogué, Amadou Hamadoun Babana, Christiane Abreu de Oliveira Paiva and Ivanildo Ivodio Murriel  

Isolation and characterization of haloalkaliphilic bacteria from the hot springs of Lake Magadi

July 2020

The Magadi Lake located in the Southern Rift Valley in Kenya is a hypersaline and alkaline lake. The lake’s pH ranges from pH 9 to 11 whereas the salinity can reach saturation during the dry Season. Little studies have previously been done on Lake Magadi as compared to other soda lakes from Kenya. This study concentrated on isolating and characterizing haloalkaliphilic bacteria found in the hot springs of Lake...

Author(s): Eunice Mulango, Remmy Kasili, Romano Mwirichia, Anne-Kelly Kambura and Christabel Muhonja  

Cytological changes in Solanum lycopersicum seedlings in response to some rhizopheric fungi

July 2020

Some rhizospheric fungi of Solanum lycopersicum were isolated from Cairo governorate and identified in Taxonomy Department, Ain-Shams University, Cairo City, Egypt. These isolated fungi were cultivated on soil extract medium enriched with 2% (w/v) glucose and 5% (w/v) yeast extract. The cytological effects of the metabolites of these fungi on the seeds of S. lycopersicum were examined after three different intervals (6,...

Author(s): Ahmed Fouad Afifi, Ehab Abdel-Razik Kamel, Amal Abdelsamei Elsaed, Mohamed Abdelaziz Foaad, Eman Mohamed Fawzy and Heba Ibrahim Abo-Elmagd  

Molecular characterization, biofilm production and sensitivity to the natural extract of Staphylococcus spp. from subclinical mastitis in goats

July 2020

Mastitis is one of the most important diseases in dairy milk systems. Molecular studies of the principal agents that are involved, as well as tests of bacterial sensitivity to plant extracts, are currently the most common forms of research related to this disease. The aim of the present study was to molecularly characterize isolates from cases of subclinical mastitis and to investigate the production of biofilm...

Author(s): Rodolfo de Moraes Peixoto, Renata de Moraes Peixoto Araújo, Werônica de Souza Rocha, Luciana Jatobá e Silva Peixoto, Gisele Veneroni Gouveia, Jackson Roberto Guedes da Silva Almeida, Helinando Pequeno de Oliveira and Mateus Matiuzzi da Costa  

Comparison between wet and semi-dry anaerobic biogas diester under thermophilic and mesophilic conditions: Methane productivity and analysis of microbiota

July 2020

The current practice of using stanchion breeding cow houses results in a semi-dry manure that ideally should be used as the substrate for the anaerobic fermentation. The conventional liquid-slurry biogas fermentation is disadvantageous with high running costs due to requirement such as water addition and running efficiency, among others. This study aimed to establish a high-speed semi-dry methane fermentation system for...

Author(s): Kikue Hirota, Kosuke Hironaga, Zhihao Tu, and Isao Yumoto,

Epidemiology of pathogenic microorganisms, basic diseases and clinical biomarker analysis in patients with bloodstream infections from a 3A General Hospital in Lanzhou of China

July 2020

The aim of this study is to investigate the burden of basic diseases, the epidemiology of pathogenic microbes in patients with bloodstream infections and their clinical biomarkers. Data on bloodstream infections from 2012 to 2018 were obtained from a 3A General Hospital in Lanzhou of China. 1990 patients with bloodstream infections participated in the study; their basic diseases, pathogenic microorganisms and...

Author(s): Feng Qiangsheng, Song Yuejuan and Ha Xiaoqin  

Suitability of bacterial fermentation and foil packaging of condiment from African mesquite (Prosopis africana) seeds for nutritional retention and commercialization

July 2020

The process for bacterial fermentation and foil packaging of condiment (‘Okpeye’) from African mesquite seeds was described and its nutritional enrichment and packaging improvement for commercialization were verified. The seeds (1 Kg) were cleaned, washed and boiled in autoclave at 121°C for 2 h, cooled, drained, de-hulled and divided into two portions of 300 g each. One portion was placed in sterile...

Author(s): Uzodinma E. O., Mbaeyi-Nwaoha I. E. and Onwurafor E. U.

Isolation and characterization of rhizospheric microorganisms from bacterial wilt endemic areas in Kenya

July 2020

Ralstonia solanacearum is a soil-borne bacterium causing bacterial wilt, particularly in Solanaceae crops, including tomatoes. Due to pathogen’s wide host plant range, pesticide resistance, and ability to overcome cultivars utilized for innate resistance, not even a single measure is effective against the pathogen. Screening the rhizosphere of bacterial wilt tolerant tomato plants from Bomet, Kiambu, Kajiado and...

Author(s): Kones C., Mwajita M., Kariuki L., Kiirika L. and Kavoo A.  

Bridging technique failure through low-tech improvisation: A case study of food microbiology

July 2020

Modern technology for food safety studies includes standardized protocols and equipment. However, appropriate technology needs to step in to bridge technology dys- or malfunctioning. We examined different low-tech methods for extraction of bacteria from fresh vegetables. Standard equipment including stomacher and filter bags were compared to extraction using bread stick and alternative filter material (nylon stocking,...

Author(s): Most Tahera Naznin, Sofia Windstam, Rumana Akter, Lars Mogren, Hanna Y. Berhane, Yemane Berhane, Eva-Charlotte Ekström, Magnus Jirström and Beatrix W. Alsanius  

Antimicrobial activity of coconut water, oil and palm kernel oils extracted from coconut and palm kernel on some plasmid-mediated multi-drug resistant organisms associated with food spoilage

July 2020

This study is aimed at evaluating the antibacterial and anticandidal activity of coconut oil, fermented and unfermented coconut water and palm kernel oil (PKO) on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Corynebacterium sp. and Candida albicans. The coconut water was fermented with Saccharomyces cerevisiae and the pH, sugar and alcohol contents were determined before and after fermentation. The...

Author(s): Akpomie, Olubunmi Olufunmi, Akponah, Ejiro, Ehwarieme, Ayobola Daniel and Paul, Rachael Efem  

GC-MS analysis of bioactive compounds and evaluation of antimicrobial activity of the extracts of Daedalea elegans: A Nigerian mushroom

June 2020

Phytochemicals are naturally occurring compounds present in varying levels in plants and help significantly in protecting them against pathogens. The chemical composition of acetone and ethanolic extracts of Daedalea elegans were determined using Gas Chromatography-Mass Spectrometry. Twenty-eight and fourty-four compounds were identified in the acetone and ethanolic extracts respectively. Only four compounds were found...

Author(s): Grace Oluwatoyin Mensah-Agyei, Kolawole IfeOluwa Ayeni and Cajethan Onyebuchi Ezeamagu  

Diversity of arbuscular mycorrhizal fungi (AMF) associated with cotton (Gossypium hirsutum L.) growing in the Far-North region of Cameroon

June 2020

The present study was carried out with the aim of highlighting the diversity of arbuscular mycorrhizal fungi (AMF) associated with the rhizosphere of cotton grown in the Far-North region of Cameroon. To achieve this, composite soil and root samples were taken in six fields, chosen according to edapho-climatic conditions and types of cultural practices. After entrapping the glomales in the greenhouse, the roots of the...

Author(s): Ismael Haman Ramza, Honore Issa Koulagna, Abaka Abba Said, Philippe Kosma and Clautilde Megueni  

Phenotypic detection of extended spectrum β-lactamase and Metallo β-lactamase production by Gram negative uropathogens after exposure to gamma radiation

June 2020

Eighty-five uropathogen isolates were collected and differentiated on the basis of their Gram stain reaction. Among the collected isolates, 25 (29.4%) were Gram positive cocci and 60 (70.6%) were Gram negative bacilli. Antibiotic susceptibility profile towards 15 different antibiotics concluded that impenime (IMP), amikacin (AK) and Pipperacillin/tazobactam (TZP) were the most effective against Gram negative; while...

Author(s): Yehia Ahmed El-Zawahry, Lotfy Abd-El-Rehem Mousa, Eman Araby and Salma Abd El Monsef-Elnahass  

Isolation of an emerging thermotolerant medically important Fungus, Lichtheimia ramosa from soil

June 2020

Lichtheimia ramosa, a ubiquitous clinically important mould was isolated during a screen for thermotolerant fungi obtained from soil on a freshly burnt field in Ikorodu, Lagos State. In the laboratory, as expected it grew more luxuriantly on Potato Dextrose Agar than on size limiting Rose Bengal Agar. The isolate had mycelia with a white cottony appearance on both media. It was then identified based on morphological...

Author(s): Imade Yolanda Nsa, Rukayat Odunayo Kareem, Olubunmi Temitope Aina and Busayo Tosin Akinyemi  

Fungal diversity within organic and conventional farming systems in Central Highlands of Kenya

June 2020

Fungal diversity in agro-ecosystems is influenced by various factors related to soil and crop management practices. However, due to the complexity in fungal cultivation, only a limited number has been extensively studied. In this study, amplicon sequencing of the Internal Transcribed Spacer (ITS) region was used to explore their diversity and composition within long-term farming system comparison trials at Chuka and...

Author(s): Edward Nderitu Karanja, Andreas Fliessbach, Noah Adamtey, Anne Kelly Kambura, Martha Musyoka, Komi Fiaboe, and Romano Mwirichia  

Presence of phytopathogenic fungi and oomycetes on rice and avocado crops in Tolima (Colombia)

June 2020

Tolima is one of the largest zones in Colombia where avocado and rice are produced. The disease management programs for these crops are mainly chemical-dependent and tend to have variable results since the diagnosis of some diseases is based on unspecific symptoms. In this context, the present study aims to bring information about the phytopathogens affecting Tolima´s rice and avocado crops. From foliar tissue,...

Author(s): Vinchira-Villarraga Diana Marcela, Macias-Camacho Jeimy, Mendez-Olivera Juan David, Méndez-Tibambre María Elizabeth, Rodríguez-García Victoria, Saavedra-Orduz Zeidy, Torres-López Michael Alejandro and Moreno-Sarmiento Nubia  

Evaluating the efficacy of household filters used for the removal of bacterial contaminants from drinking water

June 2020

Due to the erratic nature of microbial contaminants in drinking water, private and municipality water supply systems failed to deliver safe drinking water to households. In Ethiopia, there is lack of data and knowledge on the effectiveness of filter devices used to treat drinking water at household. This study aims to evaluate efficiency of household point of use filter devices (membrane filter, membrane with activated...

Author(s): Bayable Atnafu Kassa, Adey Feleke Desta and Fassil Assefa  

First novel phages from rodents with lytic activity on clinical Enterobacteriaceae strains: Initiation for phage therapy in West Africa

June 2020

Bacteriophages (phages) are viruses that infect bacteria. The emergence of resistant bacteria has been reported worldwide. The use of phages to treat bacterial infection or to reduce bacteria load has become a new area of interest. The viral colonization of rodents represents an important source for a specific biotope. The objective of this study is to characterize the effect of novel phages isolated from rodents...

Author(s): Kakou-Ngazoa E. Solange, Addablah A. Audrey, Krylova Katerina, Saraka Daniel, Kouassi K. Stéphane, Coulibaly Ngolo David, Sina Kouamé Mireille, Aoussi Serge, Dozois Charles and Dosso Mireille

In vitro efficacy of Trichoderma asperellum and detached leaflet assay on late blight pathogen: Phytophthora infestans

May 2020

Late blight is highly variable adapting to new fungicides and overcoming host resistance. The objective of the study was to determine efficacy of Trichoderma asperellum against Phytophthora infestans and its compatibility with fungicides. T. asperellum at 33% (3 × 106), 66% (7 × 106) and 100% (1 × 107 CFU/mL; g/L),   Ridomil® (Metalaxyl 4% + Mancozeb 64%) and Mistress 72® (Cynamoxil...

Author(s): Kilonzi J. M., Mafurah J. J. and Nyongesa M. W.  

Assessing the polymorphism of DHFR gene from Plasmodium falciparum in the south of Côte d’Ivoire

May 2020

Since 2005, Côte d'Ivoire has adopted new strategies of malaria management including free provision of Artemisinin-based Combination Therapy (ACT) to children less than five years of age and sulfadoxine-pyrimethamine (SP) as Intermittent Preventive Treatment (IPT) for pregnant women. However, introduction of ATCs raises concerns about the extensive use of cheap SP which could increase Plasmodium falciparum...

Author(s): Dagnogo Oléfongo, Ako Aristide Berenger, Bla Kouakou Brice, Dago Dougba Noel, Coulibaly N&#;golo David, Coulibaly Baba, Touré Offianan André and Djaman Allico Joseph,  

Naftifine inhibits pigmentation through down-regulation on expression of phytoene desaturase gene CAR1 in Rhodotorula mucilaginosa

May 2020

Naftifine, an antifungal drug, inhibits pigmentation in Rhodotorula mucilaginosa. However, the relative mechanism is minutely understood. In this study, regulation of gene expression by naftifine was investigated to elucidate mechanism of yeast de-pigmentation. RNA-sequencing (RNA-seq) was used to screen differentially expressed genes (DEGs), followed by quantitative PCR (qPCR). The qPCR results showed that mRNA...

Author(s): Guowang Huang, Nur Fazleen Binti Idris, Yimin Li, Yang Wang and Zeng Tu  

Antibacterial resistance patterns of bacteria isolated from clinical specimens at Uttara IbnSina Diagnostic Centre, Dhaka

May 2020

Nowadays, antibiotic resistance is a global public health threat. Bangladesh is accelerated to this owing to its sub-standards healthcare along with the self-medication and overuse of antibiotics. The study aimed to assess patterns of antibacterial resistance in the clinical samples. The study was carried out at Ibn Sina Diagnostic and Consultation Center Uttara, Dhaka, from January to December 2019. All cultures and...

Author(s): Rashid Md Haroon, Md Motiur Rahman, Hafiza Sultana, Md Khorshedul Islam, M. M. Nahid Al Rakib, Muhammad Abul Kalam and Syeda Sumaiya Efa

Occurrence of multidrug-resistant bacteria in aquaculture farms in Côte d’Ivoire (West Africa)

May 2020

Aquaculture provides a significant proportion of the fish consumed around the world. In West Africa, aquaculture is an important economic sector. However, several diseases with high fish mortality are caused by bacterial infections.  Due to the lack of surveillance in aquaculture, this study investigated the presence of bacteria in fish farms. The purpose of the study was to isolate bacteria in aquaculture (Ivory...

Author(s): Amian Aristide KOUDOU, Solange KAKOU-NGAZOA, Kouadio Fernique KONAN, Edwige AKA, Audrey ADDABLAH, David COULIBALY N’GOLO, Stéphane KOUASSI, Mireille Kouamé SINA, Hortense ATTA DIALLO, Nathalie GUESSEND, Serge AHOUSSI and Mireille DOSSO

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