Antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat
July 2021
The zoonotic potential of Escherichia coli from chicken and beef food products is well documented. The uses of antibiotics on agriculture encourage the development of resistance bacteria capable of causing human disease and passing resistance to human pathogens. This study aimed to detect the antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat. E....
Partial chemical characterization of the yeast extracts Lachancea thermotolerans CCMA 0763
July 2021
The fungi are living organisms of great importance around agriculture, food industry, and the pharmaceutical industry. The usage of these microorganisms in agriculture is for the biocontrol of diseases in plants of commercial interest. Some yeasts can fight phytopathogens through secondary metabolites produced, inhibiting their development. Some species show positive results in the control of pathologies in...
Antifungal and antibacterial activity of the different parts of mature Benincasa hispida against various microbial infectious agents
July 2021
In this study we assayed on antimicrobial activity of peel, pulp, waxy coating of Benincasa hispida. Various parts of the plant are reported as antibacterial agents worldwide. Leaves, flower, root, seed are the most studied elements as antibacterial agent. Some fruits are also proved as antibacterial agent and used as herbal medicine as well as nutritional supplements during disease. Different parts of mature and...
Molecular diagnosis of multidrug-resistant tuberculosis from culture-positive isolates using line probe assay in North Karnataka
July 2021
The control of tuberculosis (TB) has become a global health challenge due to the emergence of multidrug-resistant tuberculosis (MDR-TB) in Mycobacterium tuberculosis (MTB). This highlights the need for faster and more accurate detection of tuberculosis cases. The study aims to detect MDR-TB strains of pulmonary tuberculosis using resistance ratio method and to compare the diagnostic value of drug susceptibility...
Assessment of the physicochemical qualities and microbiological profile of Idah River, Kogi State, Nigeria
July 2021
Analysis of water bodies, such as rivers and lakes can provide an insight into their intrinsic composition and potential impact on the environment. Water samples collected from four designated sites in Idah River, were assessed for their physicochemical parameters and microbial diversity using standard procedures. The temperature from all sites was 26.00°C and the pH ranged from 6.93 to 7.08. Turbidity values ranged...
Screening for the prevalence of Helicobacter pylori infection among dyspeptic patients using simple fecal antigen and serum antibody diagnostic methods at Mukalla city Hospitals, Hadhramout, Yemen
June 2021
Helicobacter pylori is the most common infection in the world, and the most main causes of dyspepsia are related to H. pylori infection. In that respect, several non-invasive methods for the diagnosis of H. pylori infection were utilized. The current study aimed to determine the prevalence of H. pylori infection and its associated variables among dyspeptic patients in Mukalla city, Hadhramout-Yemen during a period from...
Probiotic properties of Lactobacilli isolated from human milk and their inhibitory effect on gastrointestinal pathogenic bacteria
June 2021
Breast feeding has critical effects on the newborns and either mother’s health. Some of such health-improving effects of the mother’s milk is associated with the beneficial microbes, lactic acid bacteria (LAB), which are normally present in the mother’s milk. Thus, human milk-associated lactobacilli were isolated in this study and some of their probiotic properties was investigated. Afterwards,...
Prevalence and factors associated with Staphylococcus aureus nasal colonisation in orthopaedic patients at a tertiary care hospital in Kenya
June 2021
Staphylococcus aureus is an important organism in orthopaedic practice as it is the most common cause of orthopaedic infections including surgical site infections (SSIs), osteomyelitis and septic arthritis. Carriers of S. aureus are predisposed to developing invasive staphylococcal infections. Knowledge of a patient’s carrier status before surgery together with interventions to eliminate the carrier state have...
Shelf stability of processed cocoyam flour during storage at room temperature (28.0 ± 2°C) for a period of four months
June 2021
The shelf life study of dry milled cocoyam flour packaged in low density polyethylene was carried out for a period of 4-month. Microbiological, nutritional, physicochemical quality characteristics and aflatoxin content were evaluated. The total viable bacterial counts ranged from 1.6×103 - 4.8×105 cfu/g while the total viable fungal count increased from 5.0 × 101 - 3.8 × 105 sfu/g. The...
Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Children's Hospital
June 2021
Carbapenems are group of extended-spectrum β-lactam antimicrobials frequently used for treating multidrug-resistant Gram-negative bacilli (GNB) infections. This study aimed at detecting and characterizing carbapenem resistance (CR) genes among GNB isolated from patients treated in neonatal intensive care unit (NICU) of Mansoura University Children's Hospital (MUCH), Egypt. It is a prospective study conducted...
Monitoring of physicochemical and microbiological parameters during nonnonkoumou (artisanal curdled milk) production in Daloa, Côte d’Ivoire
June 2021
This study aimed to study physicochemical and microbiological parameters during nonnonkoumou production. A total of 15 samples were analyzed during this study at a rate of 3 samples per fermentation time (T0 h, T6 h, T12 h, T18 h and T24 h). The physicochemical analyses showed a drop in pH (from 7.03 ± 0.028 to 4.59 ± 0.021) during the 24 h of fermentation. The titratable acidity increased from 0.30...
Resistance profile of Salmonella isolated from food sold in the streets of N'Djamena, Chad
June 2021
The preparation and sale of street foods is booming in African cities in general and especially in N’Djamena. However, the hygienic failures observed during this activity constitute a source of contamination of these foods. The objective of this work is to determine the different serotypes of Salmonella isolated from food sold in the streets of N’Djamena in Chad and to test their sensitivity...
Distribution patterns of Vibrionaceae abundance on the landing stages in coastal area: Understanding the influence of physicochemical variables by using multiple linear regression models and corrgram for matrix correlation
June 2021
The present work used multiple linear regression (MLR) models and corrgram to assess the importance of environmental parameters on diversity and abundance dynamics of Vibrio sp. in waters of few landing stages in the city of Douala (Cameroon). It was recorded in all the five selected stations, the presence of four species of Vibrio namely, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio fluvialis and Vibrio...
Substitution of non-biodegradable plastic food packagings by ecological food packagings at Abomey-Calavi University (Benin): State of place and microbiological quality of packagings
June 2021
Challenging with environmental problems and health due to non-biodegradable plastic wastes, Abomey-Calavi university heads have banned the use of any Non-biodegradable Plastic Food Packagings (NPFP) on all campuses. This study aimed to verify the effective implementation of this decision and assess the microbiological quality of ecological primary food packagings used in Abomey-Calavi campus. To achieve the...
Characterization of diversity and pathogenecity of Pyricularia grisea affecting finger millet in Kenya
May 2021
Pyricularia grisea characterization is a prerequisite for species differentiation and understanding of the pathosystem, evolution and diversity of species. The aim of this study is to determine the morphological variation, pathogen virulence and molecular diversity of P. grisea isolates. Five isolates from infected heads of finger millet were collected from Bomet, Nakuru, Baringo, Busia and Machakos counties in 2019....
Isolation and in vitro screening of plant growth-promoting rhizobacteria from Solanum lycocarpum St. Hil., an endemic plant of the Brazilian tropical savannah
May 2021
Solanum lycocarpum St. Hil. is an endemic plant of the Brazilian tropical savannah (cerrado) that is capable of growing on acidic and nutrient-poor land, an ability which attracts attention to its rhizospheric microbiota, including plant growth-promoting rhizobacteria (PGPR). In this work, 131 bacterial strains were isolated from rhizosphere samples of S. lycocarpum and were tested in vitro for direct mechanisms of...
Evaluation of the efficacy of Trichoderma and Pseudomonas species against bacterial wilt Ralstonia isolates of tomato (Lycopersicum species)
May 2021
Ralstonia solanacearum causes bacterial wilt of tomato and limits the crop production, and antagonistic microorganisms use to suppress the disease, of which Trichoderma and Pseudomonas species are the most effective agents to control bacterial wilt. In the present study, attempt was made to isolate these two microorganisms to evaluate their effectiveness to control R. solanacearum, the causal agent of bacterial wilt...
Phenotypic and molecular characterization of multiple-resistant gram-negative bacteria in urine of pregnant women attending antenatal clinic of Mother and Child hospital, Ondo, Nigeria
May 2021
Phenotypic and molecular characterization of multiple antibiotic resistant Gram-negative bacteria in urine samples of pregnant women in Mother and Child Hospital, Nigeria was reported. In the study, 407 apparently healthy pregnant women were recruited. Structured questionnaire was administered to the patients to obtain their socio-demographic information and the medical history. Urine samples were collected, processed...
Role of heavy metals on antibiotic resistance properties in lactose fermenting isolates collected from textile industry effluents
May 2021
It is crucial to monitor the microbial ambience in heavy metal enriched industrial effluents that generally discharge into the environment. Therefore, the present study aimed to understand the load of heavy metals in multidrug-resistant lactose fermenting microorganisms isolating from textile industry effluents. Samples were collected from five different textile industrial canals. In most of the cases, the colony...
Role of mass gatherings in transmission of respiratory tract infections caused by Haemophilus influenzae
May 2021
Respiratory infection is one of the infections that can be transmitted in closed places due to the ease of transmission of pathogens from infected people to healthy people through droplets from coughing and sneezing, among these bacterial causes, Haemophilus influenza. Therefore, this study aimed to evaluate the prevalence of this type of bacteria among pilgrims coming from different geographical locations in the world...
A prospective study of bacterial isolates profile in infected open fractures
April 2021
The most common complication in open fractures is infection, which often escalates to sepsis, osteomyelitis, and amputations. The use of prophylactic antibiotics is one of the most effective strategies to prevent infection. The prevailing bacterial isolate patterns must guide the choice of antibiotics for both prophylactic and empiric therapy. This study aims to describe the bacterial isolate profiles in infected open...
Antibacterial activity of sweet orange (Citrus sinensis) juice extract on selected bacteria
April 2021
Plants have potentials to be developed into many new drugs yet to be discovered because of the countless chemical compositions in them. The investigation is targeted at the antibacterial activity of sweet orange juice extract on some bacteria using ethanol and ethyl ethanoate solvent to extract juice. Ditch method was used for the sensitivity testing against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae...
Bacteriological characteristics and resistance profiles of Vibrio cholerae O1 strains isolated in the Republic of Benin in 2020
April 2021
This study aims to carry out a bacteriological characterization and determine the resistance profile of Vibrio cholerae O1 strains isolated during the epidemiological season of 2020 in Benin. To achieve this goal, 43 diarrheal stool samples were analyzed. The samples were taken during the epidemic period of 2020. Bacteriological analyses consisted of enrichment of the samples in buffered peptone water followed by...
Virulence and multi-resistance of gram-negative bacilli strains isolated from some artisanal fermented dairy products sold in secondary schools in Benin
April 2021
This work aims at assessing toxin production capability and antibiotic resistance profiles of thermo-tolerant Gram-negative bacilli strains isolated from three types of fermented dairy products (yoghurt, dèguè millet and dèguè couscous). Samples collected in Abomey-Calavi and Cotonou were analyzed for microbial, biochemical and molecular parameters. Samples were contaminated with...
Molecular characterization of water-borne multi-drug resistant Escherichia coli
April 2021
Antibiotics are very important in the fight against infectious disease caused by bacteria and other microbes for decades. Today microbes have developed ways to resist antimicrobial agents targeted at them. We sought to characterize and simultaneously detect virulence genes associated water-borne antibiotic resistant Escherichia coli, obtained from water sampled from ground and surface water sources. The Analytical...
Multidimensional aspects of surgical site infections due to non-tuberculous mycobacteria
April 2021
We read the recent publication on “Surgical site infections” (SSI) by Braga et al. (2021) with great interest. Moreover this paper has sensitized the surgeons, practitioners, microbiologists and quality managers on the need to look for non-tuberculous mycobacteria (NTM) as a cause for SSIs. Though NTM is one another cause for SSIs, and scientific articles and case reports including the present...
Evaluation of factors fuelling global antimicrobial resistance and its economic and clinical burden
March 2021
The aim of the research is to assess the factors fuelling Antimicrobial Resistance (AMR) and its economic and clinical burden. For this purpose, a systematic review was conducted, which included an analysis of factors and economic and clinical burden of AMR. It was found that poorly treated sewage; discharges from antibiotic manufacturing plants, empirical prescribing, lack of antimicrobial stewardship, poor AMR...
Evaluation of yellow fever surveillance in Chad, 2015-2020
March 2021
The present study was conducted to determine the seroprevalence of yellow fever virus in patients with clinical signs of febrile jaundice giving suspicion of yellow fever and to evaluate diagnostic techniques for surveillance of yellow fever in the Republic from Chad. From January 2015 to July 2020, an observational study of virological markers was conducted in jaundice patients in Chad. Of the 1730 patients included in...
Degradation of lignocellulosic content of rice straw using aerobic cellulolytic bacteria isolated from forest soil of Bangladesh
March 2021
The present research work was conducted to enumerate and isolate aerobic cellulolytic bacteria from forest soil using carboxymethyl cellulose (CMC) as substrate. The identified isolate was further tested as potent candidate to improve the nutritional value of rice straw. The forest soil was loaded with substantial amount (2.45×107) of cellulolytic bacteria. A total of 10 cellulose degrading bacteria (CDB) were...
Rapidly growing mycobacterial infections associated with plastic surgery: An epidemiological description
March 2021
Surgical site infections (SSI) caused by rapidly growing mycobacterial (RGM) have become increasingly frequent. The aim of this study is to describe the epidemiological, clinical aspects and factors associated with RGM infections related to plastic surgery. Notifications of SSI from 86 health care facilities of the capital of a Brazilian state within nine years, approximately, were assessed. RGM, predominantly M....
Antimicrobial activities of microbially-synthesized silver nanoparticles against selected clinical pathogens in Akure, Nigeria
March 2021
Silver nanoparticles (AgNPs) have stimulated interest of scientists due to their wide range of applications, including their potential antimicrobial activity. This study investigated the antimicrobial activities of AgNPs synthesized by rhizospheric soil and fish pond sediment microorganisms against selected clinical pathogens. The samples were cultured and organisms identified in accordance with standard procedures. The...
Anti-quorum sensing and anti-biofilm activities of Securidaca longepedunculata Fresen, an endangered species from Burkina Faso
March 2021
The emergence of bacterial resistance to antibiotics is a serious challenge to the global health system. The QS inhibition is one of the approaches to fight against antibiotic resistance in bacteria. Securidaca longepedunculata is a medicinal plant that roots are the only organ used against microbial diseases. This species is threatened with extinction due to the massive use of its roots in phytotherapy. In this study,...
Occurrence of virulent and antibiotic resistant Staphylococcus species in ready-to-eat Rhynchophorus phoenicis and Archachatina marginata vended along the Port Harcourt-Bayelsa route
February 2021
This study was aimed at determining the occurrence of Staphylococcus species in ready-to-eat (RTE) Rhynchophorus phoenicis (edible larvae) and Archachatina marginata (land snail) vended along Port Harcourt-Bayelsa route. Eighty samples from four locations were analysed employing standard techniques for proximate and microbiological analyses; virulence determination and antibiotic susceptibility. Data were statistically...
Identification of recombinants of hepatitis B virus genotypes C from Hong Kong China
February 2021
Hepatitis B virus (HBV) infections severely threaten the health of the human being. Frequent recombination of HBV within or between genotypes was reported to favor the viral evolution and adaption and the recombination may cause more severe clinical symptoms. In the study, we collected genotype C HBV from five Asian countries and detected their possible recombination events by bioinformatic analysis. There are two main...
Evaluation of cypermethrin insecticides on the growth of some selected soil bacteria isolated from Makurdi, Middle Belt, Nigeria
February 2021
In the present investigation, a total of 30 soil samples were collected from five different sites in Makurdi metropolis to investigate the effect of cypermethrin on some soil bacteria. Physicochemical properties of the soil samples from various sites were determined using standard procedures. Some selected soil bacteria such as Escherichia coli, Klebsiella spp., Bacillus spp., Staphylococcus spp. and Proteus spp. were...
Molecular identification of genotypes of Hepatitis B virus in Borno State, Nigeria
February 2021
Hepatitis is a significant disease of the liver caused by different genotypes of hepatitis B virus. Neonates delivered of HBsAg and HBeAg positive mothers are most susceptible. The sequelae of infection and prognosis vary with the age of patient infected and the genotype (A-J) of the virus involved. These genotypes have 7.5 to 8% difference in their genomic sequences. This study was aimed at identifying the genotypes...
A study of bacterial pathogens associated with diarrhoea in children under 2 years in Ile-Ife, Nigeria
February 2021
Diarrhoea, a major contributor to high mortality in children may be caused by an array of bacterial, viral or parasitic pathogens acting individually or synergistically. This study identified the bacterial pathogens associated with diarrhoea in children aged 0-24 months, established the frequency of diarrhoea caused by each pathogen encountered in diarrhoea and determined the antibiotic resistance pattern of the...
Mycorrhizal activity as a quality indicator in the use of mining slag as soil conditioner
February 2021
The main residue of iron and nickel mining is slag, a solid residue of low solubility and rich in magnesium silicate. A residue with potential use as a soil conditioner, however, it is still necessary to investigate whether its use brings impacts to the environment. Microorganisms are extremely sensitive to environmental changes, changes that can be used as environmental quality indicators. Among the organisms that act...
Isolation, identification and growth conditions of calcite producing bacteria from urea-rich soil
January 2021
Bacterial chemical reactions, such as urea hydrolysis can induce calcium carbonate precipitation. The induced production of calcium carbonate formed by microorganisms has been widely used in environmental and engineering applications. The present study aimed to isolate, identify and optimize growth conditions of urease positive bacteria from urea rich soil in Gaza Strip. Bacterial isolates, which tolerated ≥10% urea...
Comparison of disk-diffusion and E-test methods for in-vitro susceptibility of Streptococcus pneumoniae isolates to oxacillin and ceftriaxone
January 2021
Susceptibility of Streptococcus pneumoniae (pneumococci) to oxacillin and ceftriaxone was compared using disk diffusion and E-test methods. A total of 206 children attending Gertrude’s Children’s Hospital (GCH) were recruited. Sterile Copan Flocked Swabs were used to obtain nasopharyngeal swabs. Samples were inoculated on gentamicin blood agar and initial identification was done on the basis of colony...
Isolation and Characterization of Bacteria from Lakes Olbolosat and Oloiden, Kenya
January 2021
There is still unexplored reservoir of microorganisms from sediments and water within Lakes Olbolosat and Oloiden using culture dependent technique. The current study compares bacterial diversity within Lake Olbolosat a freshwater lake and Lake Oloiden a saline alkaline lake. Out of 60 isolates obtained from sediments and water samples, 35 were from Lake Olbolosat and 25 from Lake Oloiden. Microbial count ranged between...
Viruses, coronaviruses and COVID-19: A note for non-virology specialists
January 2021
An outbreak of a respiratory disease with severe acute respiratory syndrome (SARS) – like manifestations emerged in late December 2019 in the Wuhan city of China. The causative agent of this disease was later identified to be a novel Coronavirus. Subsequently, the disease was named coronavirus disease 2019 (COVID-19) by the World Health Organisation and later declared as a pandemic. The outbreak of COVID-19 came...
Antibiotic resistance in food producing Animals in West Africa French speaking countries: A systematic review
January 2021
This review aimed to make an inventory of the relevant work carried out on antibiotic resistance in the animal sector during the last two decades in French-speaking countries of the West African sub-region. English and French published articles from 2000 to 2019 indexed in PubMed, Google Scholar, and African Journals Online were reviewed in accordance with an adapted PRISMA guideline. Mean Resistance (MR) and...
In-vitro assessment of antibacterial activity of crude methanolic and aqueous extracts of Mitracarpus villosus
January 2021
Globally, there is an increasing report of bacterial resistance to currently available antibiotics. The urgent need for newer therapeutic modalities was the incentive for the current evaluation of bactericidal and inhibitory effects of methanolic and aqueous extracts of Mitracarpus villosus on selected antibiotic resistant Gram-positive and Gram-negative bacteria. Ten-fold serial dilution was used to test the...
Evaluation of epidemiological factors of the bacterial pharyngitis in children in Shouk Al-Kameesh, Al-Khoms, Libya
January 2021
Pharyngitis is an inflammation or irritation of the pharynx or palatine tonsil. The etiology is usually viruses which cause the majority of the pharyngitis but most bacterial cases are attributed to group A Streptococci (GAS). This study aimed to identify the frequency of bacterial pharyngitis especially GAS of children, according to epidemiological factors. Symptoms present and signs for each patient were recorded,...
Genome codon bias analysis of dengue virus type 1
December 2020
Dengue viruses (DENV) are the most common mosquito-borne RNA virus with high variation and adaptation in tropical and subtropical regions. Exploration of codon usage bias of DENV can be significant to understand their genetic variation and adaptation. In the study, the codon usage pattern of dengue virus type 1 (DENV-1) was analyzed by using codonW, CUSP and SPSS. The extent of codon preference of DENV-1 is weak with a...
Antibacterial abilities of spray sanitizer solutions formulated with chitosan and acid complexes at pH 3 on broiler carcass surfaces inoculated with selected pathogenic bacteria before refrigeration
December 2020
Single acid (acetic acid, lactic acid, propionic acid and phosphoric acid) and acid complex solutions at the ratio 1:1 or 2:1 at pH 3 were investigated their antimicrobial activities against three selected foodborne pathogens (Escherichia coli, Salmonella typhi and Staphylococcus aureus). The influences of the deacetylation degrees (DD) (80% and 95%), concentrations (500, 1000, and 2000 μg/mL) and contact time (10,...
Antibiotic resistance of enterobacteria (Escherichia coli, Klebsiella spp. and Salmonella spp) isolated from healthy poultry and pig farms in peri-urban area of Lome, Togo
December 2020
This study aimed to determine for the first time the levels and patterns of antimicrobial resistance of enterobacteria isolated from poultry and pigs farms in southern Togo. A cross-sectional study was conducted in south Togo in 70 and 47 poultry and pig farms, respectively. Fecal samples were collected once in each farm and enterobacteria isolated according to recommended techniques. Isolates from each sample were...
Host-microbe-drug triad: Role of chloroquine/hydroxychloroquine in Covid-19 treatment-focus on inflammatory cytokine inhibition
December 2020
The threat caused by the recent coronavirus disease 2019 (COVID-19) virus pandemic has thrown everyone into a panic mode including scientists, medical practitioners and pharmaceutical firms trying to discover a drug for its treatment. This has seen many clinical studies registered within the past few months. This has called for repositioning of some drugs in order to manage the crisis with hydroxychloroquine and...
Nosocomial pulmonary infections at Peltier Hospital, Djibouti
November 2020
Nosocomial pulmonary infections are a major public health problem. The retrospective study was carried out on medical records of 140 patients aged 59.4±32.2, 0.29 (mean ± sd, SEM) years, admitted at Peltier hospital, Djibouti, between May 5, 2018 and April 30, 2020, and who developed nosocomial pulmonary infections after 48 h of admission. The objective was to establish incidence of nosocomial pulmonary...
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