Characterization of culturable microbial community in oil contaminated soils in Greater Port Harcourt Area, Nigeria
January 2022
The study investigated microbial population dynamics in soils of Greater Port Harcourt Area, Nigeria. The study involved the quantification of petroleum pollutants and the enumeration and characterization of culturable fungi and bacteria. Gas Chromatograph FID method was used to quantify total petroleum hydrocarbons (TPH) in the soil while morphological and biochemical tests were used to characterize the microorganisms....
Antibiotic-producing bacteria isolated from some natural habitats in the Federal Capital Territory (FCT), Nigeria
January 2022
High global prevalence of multidrug-resistant bacteria due to antibiotics misuse has prompted the need for novel antibacterial compounds to replace the failing antibiotics. This study investigated some natural habitats in Abuja, Nigeria, for antibiotics-producing bacteria. Thirty-six soil samples from termite mounds, river banks and rhizospheres of Anacardium occidentale L. (cashew tree), Gmelina arborea Roxb. ex Sm....
Microbiological, nutritional and sensory evaluation of snack bars developed using Bambara groundnut (Vigna subterranean L.) and maize (Zea mays)
January 2022
Over the years, snacking has become a regular habit for majority of the population with increasing need for food manufacturers to meet consumers’ demand through product development. In this study, snack bars were prepared using maize and Bambara nuts mixed in the following ratios: A, 100% maize; B, 100% Bambara nuts; C, 50% maize: 50% Bambara nuts; D, 75% maize: 25% Bambara nuts; E, 75% Bambara nuts: 25% maize....
Impact of mycorrhization on transplanting stress and the juvenile growth of an Ivorian forest species Guibourtia ehie (Fabaceae, (A. Chev.) J. Leonard)
January 2022
Ivorian forest has been experiencing degradation for several decades despite reforestation efforts. The main cause of the failure of reforestation policies is an important mortality at the time of planting (transplanting stress). To remedy this problem, mycorrhization technology based on the beneficial effects of arbuscular mycorrhizal fungi (AMF) could provide a sustainable solution. The objective of this study was to...
Isolation and identification of cellulose-degrading endophytic bacteria from Tomoceridae (springtails)
January 2022
Springtails are considered as an important candidate bioindicator to assess soil quality spiked with trace metals, but little is known of their endophytic bacteria. In this study, a kind of Tomoceridae springtail was used, and a total of 45 effective sequences were obtained through the process of endophytic bacteria isolation, culture, polymerase chain reaction (PCR) amplification and sequencing. After NCBI-BLAST, the...
Notice of Retraction: General steps in the production and establishment of transgenic chicken lines carrying a bacterial lacZ gene expressing ?-galactosidase
December 2021
The editors of the African Journal of Microbiology Research (AJMR) are retracting the article, “General steps in the production and establishment of transgenic chicken lines carrying a bacterial lacZ gene expressing β-galactosidase” published in 2012 (Vol. 6(20), pp. 4344-4354, 30 May, 2012). This is because the data published were duplicated from a dissertation (submitted to the Graduate...
Taeniasis-cysticercosis complex and tuberculosis in food
December 2021
Although in 2020 the country's Gross Domestic Product (GDP) declined 4.1% compared to the previous year, the participation of livestock in the same period increased its representation from 8.4 to 10%. It is estimated that Brazil's contribution to the world food market has grown from 20.6 billion to 100 billion and the prospects are for greater increases for the coming years. In terms of people fed, the numbers...
Molecular characterization of high-risk infection vaginal bacteria isolated from pregnant women in CHU-MEL of Cotonou (Benin)
December 2021
The aim of this study is to determine the distribution of the genotypic pathogenicity traits of vaginal high-risk infectious bacteria (HRIB) collected in the CHU-MEL of Cotonou (Benin). To achieve this, a recto-vaginal swab of 42 pregnant women in the third trimester of pregnancy was collected. Species identification was carried out by specific biochemical tests. Antimicrobial susceptibility was tested according to the...
Fungal contamination of dental appliances: A cross- sectional study
November 2021
Orthodontic treatment makes necessary to use a longtime fixed-band appliance, it offers suitable conditions for fungal growth, dentures also allow fungal contamination on their surfaces. The aim of our study is the identification of the fungal species colonizing surfaces of dental appliances, the cross-sectional study was carried out on 60 patients wearing dental appliances, during 5 months, and sterile swabs were used...
Antibiotic resistant pattern of bacteria in untreated hospital wastewaters from Offa Local Government Area, Kwara State, Nigeria
November 2021
This study determined the prevalence and drug resistant patterns of bacteria isolated from untreated hospital wastewaters collected from selected hospitals in Offa Local Government Area of Kwara State, Nigeria. A total of 42 composite samples were aseptically collected, transported and analyzed for enumeration of microorganisms, bacteriological identification and susceptibility testing following standard procedures. The...
Diagnostic value of two HIV rapid diagnostic tests 16 years after their adoption in national strategies in the Central African Republic (CAR)
November 2021
The broad genetic diversity of HIV requires regular reassessment of adopted HIV diagnostic tests. The objective of this study was to reassess the performances of Determine® HIV 1/2 and Uni-gold® HIV1/2, 2 HIV rapid diagnostic tests of the national algorithm, adopted since 2005 in Central African Republic. A reference prospective panel of 150 plasma samples were tested in duplicate with Determine® HIV 1/2 and...
Etiological and predictive factors of Acute Meningitis in Mansoura fever hospital, Egypt
November 2021
Meningitis is a public health concern. It is caused by several etiologic agents that vary by age group and geographical area. This study aims to highlight the etiological and predictive factors of acute meningitis in hospitalized febrile patients in Mansoura Fever Hospital, Egypt. This study includes cases admitted with suspected meningitis. The study is conducted in the period between April 2019 and March 2020. Lumbar...
Survey on efficiency of inoculation methods of Pseudomonas fluorescens on growth and yield of Thymus kotschyanus
November 2021
Thyme is one of the most important medicinal plants in wild rangeland in Iran that has lots of benefits. Pseudomonas flourescence is one of the effective plant growth promoting bacteria (PGPR) as a bioinoculant for medicinal plants. This study aims to survey three inoculation techniques with PGPR on growth and oil content in Thymus kotschyanus for organic cultivation. For this research, an experiment...
Seroprevalence and behaviour at risk of zoonotic transmission of bovine brucellosis in Namentenga Province, Burkina Faso
November 2021
Bovine brucellosis is a major zoonosis caused by Brucella abortus. It is a disease with a high hygienic and economic impact that mainly affects breeders, handlers and consumers of dairy products and animal health professionals. It is therefore a real public health problem. The objective of this study was to assess seroprevalence and risk behaviour for zoonotic transmission of bovine brucellosis in Namentenga Province in...
Biosafety, bacteriological quality and strategy of biopreservative administration for controlling spoilage bacteria in Thai traditional dried seafood products
October 2021
Biosafety has currently become an important public health issue in seafood industry of Thailand. In order to enhance quality of seafood products, three phases of study were established. In the first phase, biosafety of traditional processed squid sold in Chon Buri province, Thailand compiled from literatures between 2002 and 2019 was evaluated. The squid products represented a health hazard due to 86.5% samples, having...
Q fever in Tunisia, an underestimated infection
October 2021
Q fever is a common zoonosis with almost a worldwide distribution caused by Coxiella burnetii. Farm animals and pets are the main reservoirs of infection and transmission to humans is usually via inhalation of contaminated aerosols. Infection in humans is often asymptomatic, but it can manifest as an acute disease (usually a self-limited flu-like illness, pneumonia or hepatitis) or as a chronic form (mainly...
Characterization of bacterial strains from bacterial culture collection of rice sheath in Burundi highlights an Alcaligenes species strain with antibacterial activity against Pseudomonas fuscovaginae rice pathogen
October 2021
The sheath rot rice disease is known to be associated to Pseudomonas fuscovaginae and a recent study on the symptomatic of sheath rot revealed that this bacterial pathogen is abundant in highland rice ecology cultivation in Burundi. From asymptomatic samples of sheath rice, a collection of bacterial isolates and culturable microbiome have been carried out. A comparison between the culturable microbiome and total...
RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Côte d’Ivoire
October 2021
The application of bacteriophages biocontrol requires the formulation of genetically distinct bacteriophages in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered a cheap, reproducible, and readily applicable tool in detecting phage diversity compared to other molecular techniques such as whole-genome sequencing. We used in this study the RAPD-PCR technique to assess the genetic diversity of...
Prevalence of SEA and SEB producing Staphylococcus aureus isolated from foodborne- outbreaks in Iran
October 2021
Staphylococcus aureus is an important pathogen causing a wide variety of diseases such as skin infections, food poisoning, pneumonia and septicemia in humans and animals. Among the bacterial agents of food poisoning, S. aureus is the most common agent worldwide. The aim of this study was to assess prevalence of SEA, SEB enterotoxin producing S. aureus isolated from foodborne outbreaks in Iran. In this study, 313...
Rabies virus in biting dogs and behaviour at risk of zoonotic transmission of rabies in Ouagadougou, Burkina Faso
October 2021
Rabies is an infectious, viral disease, usually inoculable by dog bite and common to humans and other mammals. It is a major zoonosis but neglected especially in Africa. However, it is the most serious and feared zoonosis in the world because once declared it leads inevitably to death. The objective of this study was to search for rabies virus in biting dogs received in 2020 at the veterinary clinic of the National...
Prevalence of aflatoxin M1 in pasteurized and ultra-high temperature (UHT) milk marketed in Dar es Salaam, Tanzania
September 2021
The aim of this study was to determine the level of aflatoxin M1 (AFM1) in pasteurized and UHT milk marketed in Dar es Salaam, Tanzania. AFM1 in pasteurized milk samples (75) and ultra-high temperature (UHT) milk (43) was determined by using immuno-affinity high performance liquid chromatography. AFM1 was detected in 97% (115/118) of samples that consisted of 96% (72/75) of pasteurized milk samples and 100%...
Hazard assessment and resistance profile of Escherichia coli strains isolated from bovine carcasses at the main slaughterhouses of Dakar, Senegal
September 2021
Foodborne bacterial hazards and antibiotics resistance are a public health threat. This study aimed at assessing the slaughter conditions, the bacteriological quality of bovine carcasses and at determining the antimicrobial resistance of Escherichia coli strains isolated from bovine carcasses. A questionnaire was administered to 41 stakeholders on the slaughter line. Swabbed carcasses were analyzed according to the...
Candida spp. associated with hot beverages of coffee and tea sold on street in Côte d’Ivoire
September 2021
Some Candida species are considered as human opportunistic pathogens and can play an important role in spontaneous fermentations, but also as beverage-spoiling microorganisms. The aim of this pioneering study was to investigate Candida spp. in hot beverages, which is consumed mostly on the streets in Côte d’Ivoire. Yeast strains were isolated from 400 hot beverages of tea (200) and coffee (200) samples....
Human immunodeficiency virus type 1 (HIV-1) subtype diversity in Busia, Western Kenya
September 2021
HIV infection is currently the single biggest epidemic globally. HIV the etiologic agent for AIDS is divided into two types: HIV-1 and HIV-2. HIV-2 is rare and is mainly found in some parts of West Africa. HIV-1 accounts for most cases of AIDS reported globally. HIV-1 strains can be classified into four groups: The "major" group M, group O, group N and the most recent group P. All of which may represent...
Aflatoxigenic potential of Aspergillus section Flavi isolated from maize seeds, in Burkina Faso
August 2021
The frequency of occurrence and four principal kinds of aflatoxin concentration in maize seeds grown in Burkina Faso was investigated. Ten (10) samples collected, were analyzed by high performance liquid chromatography (HPLC) with post-column derivatisation after immunoaffinity column cleanup. Eight strains of Aspergillus section Flavi were previously isolated from these samples and cultivated on “Aspergillus...
Prevalence and characterization of extended-spectrum ?-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from poultry in Ouagadougou, Burkina Faso
August 2021
This study aimed to determine the prevalence of extended spectrum beta-lactamase producing (ESBL) and multidrug resistant Escherichia coli and Klebsiella pneumoniae. A cross-sectional study was conducted in Ouagadougou in two poultry farms and two slaughterhouses. 375 cloacal swabs and 46 environment samples were collected and members of Enterobacteriaceae were isolated on EMB agar containing 4 µg/L of cefotaxime....
Biopreservative application of bacteriocins obtained from samples Ictalurus punctatus and fermented Zea mays
August 2021
This study evaluated the preservative ability of protein-like cell free supernatants produced by lactic acid bacteria (LAB) isolates from samples of Ictalurus punctatus (Cat fish) and slurry of fermented Zea mays (Ogi). The LAB strains were separately isolated from understudied samples using De Man, Rogosa and Sharpe (MRS) media at 37°C for 48 h. The isolated strains were characterized with Gram staining, oxidase...
Phenotypic and genotypic characterization of antibiotic resistant gram negative bacteria isolated in Tabuk City, Saudi Arabia
August 2021
Antimicrobial surveillance and identifying the genetic basis of antimicrobial resistance provide important information to optimize patient care. The present study is an analytical cross sectional study aimed to determine the prevalence of multidrug resistant (MDR), extensively drug resistant (XDR), pan drug resistant (PDR) and extended-spectrum ?-lactamases genes among Gram-negative bacteria isolated in Saudi...
Detection of Legionella pneumophila as the cause of atypical pneumonia in the water sources of the holy places of Makkah
August 2021
Legionella pneumophila is an important pathogen and is involved in more than 95% of cases of severe atypical pneumonia. The current study focused on the ablution water in the grand mosque in Makkah as well as water tanks in hotels surrounding the holy mosque. A total of 100 water samples were collected from ablution water in the Haram and the hotels in the central area around the grand mosque and sent to the...
Effect of bacteriocins from lactic acid bacteria obtained from Zea mays-based “Ogi” on foodborne bacteria from contaminated cabbage
August 2021
The objective of this work was to evaluate the effect of bacteriocins from lactic acid bacteria (LAB) in Zea mays-based Ogi on some foodborne bacteria contaminating cabbage in Abakaliki, Nigeria. Ten (10) samples (5 samples of Z. mays-based Ogi and 5 samples of suspected contaminated cabbage heads) were aseptically collected and analyzed using standard microbiological methods. Five different Lactobacillus isolates (A,...
Heavy metals and microbial contamination of palm oil produced and sold at some markets in Kogi East Area, Kogi State, Nigeria
August 2021
The physicochemical, microbial and heavy metal contamination of palm oil samples randomly selected from three different markets (Ankpa, Anyigba and Idah Markets) in Kogi State, Nigeria, was investigated. The results revealed the presence of moisture (0.5, 0.4, 0.4%); impurity (0.4, 0.4, 0.3%) and free fatty acid (13, 12.8, 12.3%) for Ankpa, Anyigba and Idah Markets respectively. The microbial contaminants isolated were...
Partial chemical characterization of the yeast extracts Lachancea thermotolerans CCMA 0763
July 2021
The fungi are living organisms of great importance around agriculture, food industry, and the pharmaceutical industry. The usage of these microorganisms in agriculture is for the biocontrol of diseases in plants of commercial interest. Some yeasts can fight phytopathogens through secondary metabolites produced, inhibiting their development. Some species show positive results in the control of pathologies in...
Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh
July 2021
Verotoxin-producing E. coli are getting an emergence for human health and day by day, it is also becoming more alarming in the fact that, they are acquiring multidrug-resistant profiles. This study aimed at isolation and identification of verotoxigenic E. coli followed by the antibiogram of the species from salad vegetables at Jashore district in Bangladesh. A total of 119 raw vegetable salad samples were collected from...
Antifungal and antibacterial activity of the different parts of mature Benincasa hispida against various microbial infectious agents
July 2021
In this study we assayed on antimicrobial activity of peel, pulp, waxy coating of Benincasa hispida. Various parts of the plant are reported as antibacterial agents worldwide. Leaves, flower, root, seed are the most studied elements as antibacterial agent. Some fruits are also proved as antibacterial agent and used as herbal medicine as well as nutritional supplements during disease. Different parts of mature and...
Antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat
July 2021
The zoonotic potential of Escherichia coli from chicken and beef food products is well documented. The uses of antibiotics on agriculture encourage the development of resistance bacteria capable of causing human disease and passing resistance to human pathogens. This study aimed to detect the antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat. E....
Long-term effectiveness of commonly used hospital disinfectants against Pseudomonas aeruginosa and Staphylococcus aureus
July 2021
Nosocomial infections are enhanced due to a flop in the infection control processes such as disinfection. The aim of this study was to assess long term effectiveness and the stability of disinfectants currently used within healthcare settings in Accra, Ghana against two indicator pathogens. Two locally produced and two imported disinfectants usually used in health care settings were obtained. The efficacy and long-term...
Assessment of the physicochemical qualities and microbiological profile of Idah River, Kogi State, Nigeria
July 2021
Analysis of water bodies, such as rivers and lakes can provide an insight into their intrinsic composition and potential impact on the environment. Water samples collected from four designated sites in Idah River, were assessed for their physicochemical parameters and microbial diversity using standard procedures. The temperature from all sites was 26.00°C and the pH ranged from 6.93 to 7.08. Turbidity values ranged...
Molecular diagnosis of multidrug-resistant tuberculosis from culture-positive isolates using line probe assay in North Karnataka
July 2021
The control of tuberculosis (TB) has become a global health challenge due to the emergence of multidrug-resistant tuberculosis (MDR-TB) in Mycobacterium tuberculosis (MTB). This highlights the need for faster and more accurate detection of tuberculosis cases. The study aims to detect MDR-TB strains of pulmonary tuberculosis using resistance ratio method and to compare the diagnostic value of drug susceptibility...
Monitoring of physicochemical and microbiological parameters during nonnonkoumou (artisanal curdled milk) production in Daloa, Côte d’Ivoire
June 2021
This study aimed to study physicochemical and microbiological parameters during nonnonkoumou production. A total of 15 samples were analyzed during this study at a rate of 3 samples per fermentation time (T0 h, T6 h, T12 h, T18 h and T24 h). The physicochemical analyses showed a drop in pH (from 7.03 ± 0.028 to 4.59 ± 0.021) during the 24 h of fermentation. The titratable acidity increased from 0.30...
Probiotic properties of Lactobacilli isolated from human milk and their inhibitory effect on gastrointestinal pathogenic bacteria
June 2021
Breast feeding has critical effects on the newborns and either mother’s health. Some of such health-improving effects of the mother’s milk is associated with the beneficial microbes, lactic acid bacteria (LAB), which are normally present in the mother’s milk. Thus, human milk-associated lactobacilli were isolated in this study and some of their probiotic properties was investigated. Afterwards,...
Prevalence and factors associated with Staphylococcus aureus nasal colonisation in orthopaedic patients at a tertiary care hospital in Kenya
June 2021
Staphylococcus aureus is an important organism in orthopaedic practice as it is the most common cause of orthopaedic infections including surgical site infections (SSIs), osteomyelitis and septic arthritis. Carriers of S. aureus are predisposed to developing invasive staphylococcal infections. Knowledge of a patient’s carrier status before surgery together with interventions to eliminate the carrier state have...
Screening for the prevalence of Helicobacter pylori infection among dyspeptic patients using simple fecal antigen and serum antibody diagnostic methods at Mukalla city Hospitals, Hadhramout, Yemen
June 2021
Helicobacter pylori is the most common infection in the world, and the most main causes of dyspepsia are related to H. pylori infection. In that respect, several non-invasive methods for the diagnosis of H. pylori infection were utilized. The current study aimed to determine the prevalence of H. pylori infection and its associated variables among dyspeptic patients in Mukalla city, Hadhramout-Yemen during a period from...
Substitution of non-biodegradable plastic food packagings by ecological food packagings at Abomey-Calavi University (Benin): State of place and microbiological quality of packagings
June 2021
Challenging with environmental problems and health due to non-biodegradable plastic wastes, Abomey-Calavi university heads have banned the use of any Non-biodegradable Plastic Food Packagings (NPFP) on all campuses. This study aimed to verify the effective implementation of this decision and assess the microbiological quality of ecological primary food packagings used in Abomey-Calavi campus. To achieve the...
Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Children's Hospital
June 2021
Carbapenems are group of extended-spectrum β-lactam antimicrobials frequently used for treating multidrug-resistant Gram-negative bacilli (GNB) infections. This study aimed at detecting and characterizing carbapenem resistance (CR) genes among GNB isolated from patients treated in neonatal intensive care unit (NICU) of Mansoura University Children's Hospital (MUCH), Egypt. It is a prospective study conducted...
Shelf stability of processed cocoyam flour during storage at room temperature (28.0 ± 2°C) for a period of four months
June 2021
The shelf life study of dry milled cocoyam flour packaged in low density polyethylene was carried out for a period of 4-month. Microbiological, nutritional, physicochemical quality characteristics and aflatoxin content were evaluated. The total viable bacterial counts ranged from 1.6×103 - 4.8×105 cfu/g while the total viable fungal count increased from 5.0 × 101 - 3.8 × 105 sfu/g. The...
Resistance profile of Salmonella isolated from food sold in the streets of N'Djamena, Chad
June 2021
The preparation and sale of street foods is booming in African cities in general and especially in N’Djamena. However, the hygienic failures observed during this activity constitute a source of contamination of these foods. The objective of this work is to determine the different serotypes of Salmonella isolated from food sold in the streets of N’Djamena in Chad and to test their sensitivity...
Distribution patterns of Vibrionaceae abundance on the landing stages in coastal area: Understanding the influence of physicochemical variables by using multiple linear regression models and corrgram for matrix correlation
June 2021
The present work used multiple linear regression (MLR) models and corrgram to assess the importance of environmental parameters on diversity and abundance dynamics of Vibrio sp. in waters of few landing stages in the city of Douala (Cameroon). It was recorded in all the five selected stations, the presence of four species of Vibrio namely, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio fluvialis and Vibrio...
Role of mass gatherings in transmission of respiratory tract infections caused by Haemophilus influenzae
May 2021
Respiratory infection is one of the infections that can be transmitted in closed places due to the ease of transmission of pathogens from infected people to healthy people through droplets from coughing and sneezing, among these bacterial causes, Haemophilus influenza. Therefore, this study aimed to evaluate the prevalence of this type of bacteria among pilgrims coming from different geographical locations in the world...
Role of heavy metals on antibiotic resistance properties in lactose fermenting isolates collected from textile industry effluents
May 2021
It is crucial to monitor the microbial ambience in heavy metal enriched industrial effluents that generally discharge into the environment. Therefore, the present study aimed to understand the load of heavy metals in multidrug-resistant lactose fermenting microorganisms isolating from textile industry effluents. Samples were collected from five different textile industrial canals. In most of the cases, the colony...
Phenotypic and molecular characterization of multiple-resistant gram-negative bacteria in urine of pregnant women attending antenatal clinic of Mother and Child hospital, Ondo, Nigeria
May 2021
Phenotypic and molecular characterization of multiple antibiotic resistant Gram-negative bacteria in urine samples of pregnant women in Mother and Child Hospital, Nigeria was reported. In the study, 407 apparently healthy pregnant women were recruited. Structured questionnaire was administered to the patients to obtain their socio-demographic information and the medical history. Urine samples were collected, processed...
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