A metagenomic analysis reveals changes in the bacterial community structure due to infection by the rice yellow mottle virus
May 2024
This research aimed to explore the pathobiome to enhance the understanding of the roles played by disease-associated microbes. A comparative analysis of microbiomes in rice samples was conducted to shed light on the potential impact of the rice yellow mottle virus on microbiome composition. Forty samples were collected from rice fields of the Office du Niger in Mali. Bacterial DNA was extracted at the LaboREM-Biotech...
Resistance profile of bacteria isolated from Broncho alveolar lavage fluid (BALF) of patients with severe acute lung disease at the University Hospital Center of Cocody in Abidjan, Côte d’Ivoire
May 2024
This study aimed to characterize the microorganisms responsible for severe acute respiratory infections in adult patients hospitalized at the University Hospital Center of Cocody in Abidjan. The biological material consisted of bronchoalveolar lavage fluid (BALF). Isolation and identification of bacterial strains, along with susceptibility testing, were performed using standard microbiological techniques. The bacteria...
Exploring the antimicrobial modulatory potential of the sap from oil palm tree
April 2024
The sap from the oil palms (Elaeis guineensis), harbours complex microbiota and provides a rich source of therapeutic metabolites. This study investigated the antibacterial modulatory activity of sap from Elaeis guineensis on selected bacteria. To test how well the sap from the oil palm tree affects bacteria, the minimum inhibitory concentration (MIC) of some common antibiotics was found by mixing the sap from the oil...
Resistance profile of urine isolate enterobacteral strains at Donka University teaching hospital in Conakry, Guinea
April 2024
The objective of this study was to describe the resistance profile of enterobacterals isolated from urine samples at the laboratory of Donka National Hospital. Urine samples were collected from both outpatients and hospitalized patients. Cultures were performed using standard techniques, strains were identified using the API 20E kit, and antibiotic susceptibility testing was carried out using the ATB™ UR EU (08)...
Mycoplasma galliscepticum: An overview
March 2024
The prokaryotic microorganism Mycoplasma gallisepticum (MG) belongs to the phylum Firmicutes, the order Mycoplasmatales, the family Mycoplasmataceae, and the genus Mycoplasma. MG parasitizes the respiratory tract of various poultry species, causing a range of symptoms from indistinct features to tracheitis and air sacculitis. Common signs include respiratory tract infection indicators such as coughing, asthma, a runny...
Characterization of novel potential probiotic strains of lactic acid bacteria from rat faeces
March 2024
This study aimed to isolate and characterise novel probiotic strains from the faeces of healthy albino Wistar rats. Lactic acid bacteria were isolated on MRS agar, and their probiotic properties were assessed through in vitro tests, including tolerance to simulated gastrointestinal juices, auto-aggregation assays, and antimicrobial activity. The antibiotic susceptibility and haemolysis tests were performed to assess the...
Microbiological evaluation and possible origins of the microbial contamination of vegetables in Ouagadougou (Burkina Faso)
February 2024
This study aimed to contribute to the health safety of widely consumed vegetables in Ouagadougou by investigating the potential contamination of onion, tomato, cucumber and lettuce with pathogenic microorganisms. A survey was conducted, involving 102 producers in fields, 102 vendors in markets, and 205 consumers. The most commonly consumed vegetables were identified as onion, tomato, cucumber and lettuce. A total of 264...
Antibiotic resistance profile of Escherichia coli isolates among patients suspected of urinary tract infections at the Souro Sanou University Hospital Centre of Bobo-Dioulasso, Burkina Faso
February 2024
This study aims to describe the antibiotics resistance (antimicrobial resistance [AMR]) profile of Escherichia coli isolates among patients suspected of urinary tract infections (UTIs) at the Souro Sanou University Hospital Center (SSHUC) of Bobo-Dioulasso in Burkina Faso. A descriptive cross-sectional study was conducted from June 1 to August 31, 2021 at SSUHC. An exhaustive sampling was performed and the urine samples...
Microbiological quality and safety of poultry processed in Africa: A review
January 2024
Poultry is the second most consumed meat in the world. In Africa, chicken production and processing are practiced both formally and informally, with smallholders constituting the majority in this sector. Informal practices are vulnerable to the production and processing of chicken, which is easily contaminated by pathogens such as Escherichia coli, Staphylococcus aureus, Salmonella, and Campylobacter. The growth of the...
Antibacterial activity and phytochemical investigation of leaf and root extracts of Aloe gilbertii Reynolds
January 2024
The objective of the study was to test the antibacterial activities of crude extracts of roots and leaves of Aloe gilbertii Reynolds against clinical pathogens. The crude extracts were prepared via maceration technique employing n-hexane, acetone, chloroform, dichloromethane:methanol (50:50% V/V) and methanol solvent system. The phytochemical screening tests of the dichloromethane:methanol (50:50% V/V) root extract of...
Antibiotic sensitivity patterns of nosocomial infections bacterial isolates in National Hospital in Niamey, Niger
January 2024
Nosocomial infections are recognized as a global public health issue, with causative bacteria often exhibiting high levels of antibiotic resistance. However, there is a paucity of data on multidrug-resistant bacteria in nosocomial infections in Niger. This study aimed to determine the antibiotic sensitivity of bacteria isolated from specimens of patients suffering from nosocomial infections. Samples collected from...
Molecular identification of biofilm-producing Bacillus species and yeasts isolated from food sources and their interaction with Lysinibacillus louembei strain
December 2023
The microorganisms contained in fermented foods are known to harbor metabolic products, possibly improving human and animal health. However, despite several studies on the functional effects of fermented foods, isolation and identification of the effective Bacillus species strains are still in progress. The objective of this study was to molecularly identify biofilm-producing Bacillus spp. (BPB) and yeasts from...
Acute bacterial meningitis ecosystem in Cote D'ivoire from 2012 to 2020
December 2023
Bacterial meningitis remains a health concern in meningitis belt countries. The objective of this study was to ascertain the bacterial profiles of meningitis in Côte d'Ivoire from 2012 to 2020. The study, conducted retrospectively at the National Center of Reference of Meningitis of Cote d’ivoire, analyzed 5046 cerebrospinal fluid (CSF) samples from January 2012 to December 2020. Epidemiological data...
Prevalence, antibiotic resistance of Staphylococcus aureus isolated from layer farms (eggs, droppings/litter) in the outskirts of the city of Ouagadougou, Burkina Faso
December 2023
Eggs are susceptible to microbiological contamination by various pathogens on farms, particularly those belonging to the genus Staphylococcus, which are zoonotic bacteria capable of contaminating humans, animals, and/or birds, posing a significant public health concern. In Burkina Faso, limited data exists on the prevalence of Staphylococcus aureus, prompting this study to investigate the prevalence and antibiotic...
The assessment of contamination level of multidrug resistant bacteria and antibiotic residue in chicken manure
December 2023
This study aimed to assess the contamination level of chicken litter by antibiotic residues and multidrug resistant (MDR) bacteria. A total of 28 chicken litter samples were aseptically collected in Abidjan poultry farms in December 2021. The bacteria including Salmonella, Escherichia coli and Staphyloccocus aureus were isolated on specific culture media and were identified using biochemical tests. Antibiotics...
Denaturing gradient gel electrophoresis (DGGE): An alternative culture independent method for bacterial screening in bovine milk sample
November 2023
Bovine mastitis is a disease that has a great impact on Brazilian livestock production. Some bacteria described as mastitis-causing agents are not easily cultivable in conventional media, making their diagnosis difficult. The aim of the present study was to detect bacteria present in milk from mastitic and non-mastitic quarters of dairy cattle in Rio de Janeiro-Brazil, using a culture-independent and culture-dependent...
Effectiveness of temocillin on extended-spectrum beta-lactamase producing Escherichia coli isolates from patients attending the Niamey General Reference Hospital
November 2023
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli urinary tract infections represent a major global health problem, constituting a public health emergency with high patient morbidity and mortality. Temocillin serves as an alternative antibiotic for treating these infections and conserving carbapenems. A total of 209 isolates of uropathogenic Escherichia coli were collected in the Microbiology Laboratory...
Evaluation of the impact of the efflux pump modulation process on the anti-adhesion and antibiofilm activities of analogues of natural marine compounds in a marine bacterium
November 2023
One strategy for enhancing the effect of natural marine antifouling compounds has been to combine them with coumarin-type molecules (VL2, VL17 and VL19). These molecules are known to have an inhibitory effect on the efflux pumps (EP) of a large number of Gram-negative bacteria. To this end, they have been used as adjuvants to natural marine compounds (OB1, AS194 and AS162) to enhance their antibiofilm activity. On the...
Molecular detection of pathogenic Leptospira from cattle in peri-urban areas of Addis Ababa, Ethiopia
October 2023
Leptospirosis is a bacterial, zoonotic disease caused by pathogenic Leptospira. Rodents are known to carry pathogenic Leptospira, but livestock are also important hosts. The disease is economically important in cattle, causing abortion, decreased fertility and decline in milk yield. Pathogenic Leptospira are shed in cattle urine and can survive in the environment. Only a few studies have been performed in Ethiopia to...
Effect of sanitizers combined with ultrasound on adhesion of microorganisms isolated from fish from the Amazon region
October 2023
Attachment of undesirable microorganisms to surfaces that contact food is a source of concern, since it can result in product contamination leading to serious economic and health problems. Bacteria aggregated to form biofilms are more resistant to environmental stress than planktonic cells. The objective of this paper was to evaluate the bactericidal effect of sodium hypochlorite and peracetic acid associated with...
Growth, root colonization and yield attribute responses of five groundnut (Arachis hypogaea L.) varieties toward arbuscular mycorrhizal fungal inoculation in a Senegalese agricultural soil
October 2023
The aim of this study was to isolate the most specific and effective arbuscular mycorrhizal fungi (AMF) for groundnut and to determine the degree of variability in the response of groundnut varieties to inoculation. The seeds of five varieties: 55-437, Fleur 11, Sunu Gaal, Amoul Morom, and Essamaay were inoculated individually with five AMF (Funneliformis mosseae, Rhizophagus aggregatus and Rhizophagus fasciculatus, the...
Effect of methylprednisolone on the morphology of acute lymphoblastic leukemia cell line in vitro model by Atomic Force Microscopy
September 2023
Despite the recent knowledge of the molecular mechanisms of corticosteroid drugs in lymphoid leukemia, information about the nanostructured surface morphology of the process of cell death is limited. Therefore, the main objective of this work is to analyze the effect of methylprednisolone on the morphology of B lymphoblast CCRF-SB cell line by Atomic Force Microscopy (AFM). Morphological parameters such as height, cell...
Screening groundnut rhizobia for multiple plant growth promoting activities in Ethiopia
September 2023
Rhizobia could exhibit plant growth-promoting mechanisms besides nitrogen fixation. In search of efficient rhizobia with multiple PGP traits, 72 groundnut nodulating rhizobia isolates were screened in vitro for their plant growth promoting traits like phosphate solubilization, production of indole acetic acid, ammonia, hydrogen cyanide, production of different hydrolytic enzymes and antifungal activity. About 72% of the...
Density and diversity of arbuscular mycorrhizal fungi in Anacardium occidentale L. plantations in Senegal
September 2023
Cashew nut cultivation plays an important socio-economic role in Senegal. However, it suffers from several problems, including declining soil fertility which leads to low productivity. To overcome these constraints, the use of arbuscular mycorrhizal fungi (AMF) could be a sustainable alternative. However, the positive effects of AMFs may depend on their infectious potential, density, and diversity. So far, little...
Beneficial microorganisms as affecting root development of upland rice
August 2023
Multifunctional microorganisms can significantly affect root and shoot development of upland rice seedling that could provide an increase in the crop grain yield. The objective of this study was to determine the effect of single and combined microorganism on the root and shoot development of upland rice seedlings. The experiment was arranged in a completely randomized design with treatments consisting of the upland rice...
Morphological, pathogenic and molecular characterization of fungal species associated with mango fruits in Mexico
August 2023
The characterisation of the causal agents of postharvest diseases, as well as their pathogenicity in different mango cultivars of economic importance in Mexico was evaluated. In total, 37 fungi were isolated from diseased mango (Mangifera indica L.) of the cvs Ataulfo, Hayden, Manila, Peach, Haden and creole. For the morphological characterization, the isolates were seeded in potato dextrose agar media and described by...
Phytochemical screening and antifungal activity of Cassia alata (Linn.) crude leaf extracts
August 2023
The antimycotic effect of Casia alata (Linn.) commonly used for the treatment of skin diseases by local people in Nigeria was evaluated against dermatophytes (Trichophyton tonsurans, Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Epidermophyton floccosum). Ten-fold serially diluted extract concentrations were subjected to phytochemical screening and the...
Prevalence of Bartonella spp. in rodent and shrew species trapped in Kigoma and Morogoro Regions, Tanzania: A public health concern
July 2023
Bartonella spp. bacteria are responsible for bartonellosis in humans and animals for which rodents are the main natural reservoirs. Common bartonellosis symptoms include fever, chills, weakness, and headache. This study aimed to determine the prevalence of Bartonella spp. infection in rodents and shrews of the genus Crocidura in the Kigoma and Morogoro regions of Tanzania. Blood culture and conventional PCR targeting a...
Cross-site comparison of coastal bacterial occurrences and diversity at selected sites along the North Atlantic Ocean in Monrovia, Liberia
June 2023
Coastal marine sites were examined for the occurrences and diversity of indigenous bacterial populations using high resolution 16S rRNA gene pyrosequencing approach to further understand the ecological importance of environmental variables. Samples containing slurries of seawater and sediments were collected from nine different sites along the north Atlantic Ocean in Monrovia, Liberia and were divided into three...
Multiple adaptations of bacteriophages in Lagoon Ebrie: Indicators of permanence microbiological pollution in the environment, Côte d’Ivoire, West Africa
June 2023
The Lagoon Ebrié in the city of Abidjan is a typical aquatic ecosystem that can be used to predict the existence and circulation of water pathogens. Phages are viruses that infect bacteria and can be used for the detection of their bacteria host. In this study, bacteria colonies were investigated in their natural habitat within the Lagoon by using phages as indicators. Their impact on pollution was also...
16S rRNA gene sequence analysis of the microbial community on microplastic samples from the North Atlantic and Great Pacific Garbage Patches
May 2023
The exponential increase in plastic production has led to their accumulation in the environment, particularly in oceans, polluting these environments from the shore to the open ocean and even sea ice in the pole regions. Microbial communities were compared on plastic particles, known as "Plastisphere", collected from the Atlantic and Pacific ocean gyres in the Summer of 2019 and subsequently inspected for...
Effect of Piper nigrum, Thymus vulgaris and Syzigiuim aromaticum essential oils on the microbiological and sensory quality of Pork sausages during preservation
May 2023
The use of essentials oils (EOs) as alternative of nitrites and nitrates in sausages will be proposed in this study. For this purpose, the minimal inhibitory concentration (MIC) of white Piper nigrum of Penja, Thymus vulgaris and Syzigiuim aromaticum EOs were determined in vitro against Escherichia coli ATCC25922, Salmonella enteritidis 155A and Staphylococcus aureus NCTC10652. It follows from this study that, T....
Prevalence of rotavirus group A and associated genotypes detected in calves in Southern Highlands and Eastern Tanzania
May 2023
Rotavirus group A (RVA) causes neonatal calves diarrhoea, causing massive economic losses in many countries due to deaths, treatment costs, and stunted growth. However, little is known about Tanzanian cattle's RVA burden and genotypes. The prevalence and genetic diversity of rotaviruses circulating in cattle in Southern and Eastern Tanzania regions were investigated. A total of 272 faecal samples collected from...
Molecular characterization of heavy metal resistant Proteus species
April 2023
Heavy metals are silent killer of mankind and the cause of environmental pollution. The ability of some microorganisms to resist heavy metals makes them useful in bioremediation. The aim of this study was to molecularly characterize heavy metal-resistant Proteus species isolated from the soil of a cement factory. Proteus species were tested for resistance to lead, chromium, copper and iron at concentrations 0.00, 0.25,...
Antibiotic and heavy metal resistance genes in hospital effluents and streams in Benin
April 2023
Poor effluent management is known to release antibiotic resistance genes and heavy metal resistance genes into streams. The objective of this study was to investigate the occurrence of antibiotics and heavy metals in hospital effluents and streams in Benin. The extraction of genomic DNA from multidrug-resistant bacterial strains isolated from stream and hospital effluents samples was performed according to the...
Molecular cloning and functional research in drug-resistance of transposase gene in Burkholderia pseudomallei
March 2023
Transposons are widely used in genetic engineering research and play an essential role in microbial drug resistance research. Transposase is the key to constructing efficient transposons, but no studies have reported transposons in Burkholderia pseudomallei. In this study, a new transposase gene was cloned and identified from HNBp001 isolated from Hainan province. Blast and phylogenetic tree analysis showed that the...
ICU COVID-19 patients with bacterial and fungal super-infections in Saudi Arabia
March 2023
Bacterial and fungal infections in COVID-19 patients were reported all over the world. A retrospective study was conducted to determine the prevalence of bacterial and fungal superinfections in COVID-19 positive patients between April 2020 and December 2021. This research included patients admitted in a specialized medical center in Saudi Arabia. A total of 2643 patients with confirmed diagnosis of COVID-19 via reverse...
Aflatoxins contamination in raw and roasted cashew nuts in Mtwara, Tanzania
March 2023
The aim of this work was to determine the contamination levels of raw and roasted cashew nuts sold in Masasi and Newala districts of Mtwara region in Tanzania. A total of 60 samples including 40 roasted (24 samples from Newala and 16 from Masasi) and 20 raw samples (12 from Newala and 8 from Masasi) were collected. Determination of total aflatoxins levels in raw and roasted cashew nuts samples was carried out by immune...
Phenotypic characterization and antimicrobial susceptibility testing of Klebsiella isolates from Rattus rattus captured at university of Abuja metropolis
February 2023
The aim of this study is to phenotypically characterize Klebsiella isolates from rats. A survey was conducted on 100 swaps samples obtained from captured house rats in University of Abuja main campus from May to August 2021. Isolation and identification of the Klebsiella species was done using conventional cultural and biochemical techniques. Of the 100 samples analyzed,...
Antimicrobial susceptibility profile of Enterococcus species isolated from cows with clinical mastitis and from bulk milk tanks in Brazil
February 2023
Vancomycin-resistant enterococci (VRE) have been reported as a major hazard to human and animal health, causing problems like clinical mastitis in dairy cattle and nosocomial infections in humans. The aim of this study was to determine the in vitro microbial susceptibility profile of enterococci isolated from dairy farms in São Paulo and Minas Gerais states, Brazil. A total of 310 samples of Enterococcus species...
The role of effector Ssc1 in Sclerotinia Sclerotiorum and pathogenicity of Botrytis cinerea in the early infection stages
February 2023
In this experiment, the action mechanism of the gene Ssc1 was studied in the process of interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It was found that the gene Ssc1 could enhance the pathogenicity of Botrytis cinerea through heterologous expression. Fusing the promoter, SP and CTP of Ssc1 with GFP and expressing in tobacco, it was found that the...
COVID-19 and pregnancy: Investigation of serological markers and associated factors
February 2023
COVID-19 is a major public health emergency that has recently shaken the world. Pregnant women have not been spared from this pandemic. Although there is no clinical difference between them and the general population, pregnant women are at increased risk of developing severe forms and pregnancy-related complications. The objective of this study was to investigate the presence of anti-SARS-CoV-2 antibodies and to...
Evaluation of diagnostic tests for plague in Madagascar
January 2023
Madagascar is the country that reports the most cases of plague in the world. This is an evaluation study of plague diagnosis over a 2-year period from January 2017 to December 2018. For all suspected plague cases, peripheral RDT (performed locally), central RDT (performed in the laboratory) and PCR were performed. Parameters of the diagnostic tests used, year of study, performance and study of concordance between tests...
Bacterial communities associated with the surfaces of the fresh fruits sold around Dhaka Medical College and Hospital and their anti-microbial profiles
January 2023
Fresh fruits are popular sources of healthy diets with low energy density. Since they are consumed raw, it may act as a source of foodborne disease and a reservoir for antibiotic-resistant organisms. This study aimed to determine microbial prevalence among the fruits sold around hospital along with antimicrobial profiles. Thirty-five different types of fruits were bought from around Dhaka Medical College and Hospital...
Diversity and microbiological quality of fruit juices produced in southern Benin
January 2023
The fruit juice production sector is growing rapidly in Benin's major urban centers and appears to have enormous hygienic insufficiencies. This study aimed to identify and evaluate the microbiological quality of the different fruit juices produced in southern Benin. The methodological approach to the first objective consisted of a semi-structured survey coupled with direct observations of fifty enterprises produced...
Molecular characterization of multiple antibiotic-resistant Pseudomonas aeruginosa isolated from selected hospital fomites and hands of health care workers in Ondo, Nigeria
January 2023
The study reported the molecular characterization, antibiotic susceptibility profile and the nature of resistance genes in the multiple antibiotic resistant Pseudomonas aeruginosa isolated from selected hospital fomites and hands of health care workers in Ondo, Nigeria. Various fomites and hands of health care workers were swabbed for the detection of Pseudomonas aeruginosa. Each sample was cultured separately on...
Characterization of tannase-producing bacteria in cowpea (Vigna unguiculata L.Walp)
December 2022
Bacteria populations isolated from Cowpea were screened on tannic acid agar medium and determined by zones of hydrolysis after 48 h of incubation at 35°C with three bacteria isolates with isolate codes; DVN 5, DVN 8 and DVN 17a, identified as Enterococcus faecalis, Enterococcus sp. and Staphylococcus sp. respectively observed as having the highest zones of hydrolysis and subjected to further screening. Tannase...
Molecular analysis of zoonotic pathogens observed in Thryonomys swinderianus (Marsh cane rat) in the city of Daloa in Center Western Côte d’Ivoire
December 2022
Bush meat is a source of food and income for many people in Center Western Côte d’Ivoire. However, it can have adverse effects on the health of the population when food safety practices are not respected. The assessment of the health risk associated with the consumption of bush meat was carried out on 17 faeces samples of Thryonomys swinderianus (marsh cane rat) collected in the city of Daloa in Center...
Antimicrobial properties of Moringa Stenopetala seed oil
November 2022
Moringa stenopetala is a multipurpose tree with considerable economic and social potential as it has vital nutritional, industrial, and medicinal applications. The study was aimed to investigate the antimicrobial activities of M. stenopetala seed oil against pathogenic microorganisms. M. Stenopetala seeds were collected from three locations (Damba Gofa, Shelle, and Konso) and extracted using two different solvents...
Performance of the PanbioTM COVID-19 Ag Rapid Test in a health care setting in Ouagadougou, Burkina Faso
November 2022
This study aimed to evaluate the performance of the PanbioTM Covid-19 Ag Rapid Test (Abbott) in a medical center in Ouagadougou. The PanbioTM COVID-19 Ag test was evaluated from January 26 to March 31, 2021 in symptomatic and asymptomatic patients in the medical Centre of Kossodo. A total of 268 individuals were tested by both SARS-CoV-2 RT-PCR, and antigen RDT. Of these 268 individuals, 52 were positive and 216 were...
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