African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

AJB Articles

Study of oxygen transfer processes improvement for domestic wastewaters treatment

March 2019

In aerated processes, the oxygen transfer was limited by the presence of the suspended matter as colloid and the dissolved matter, which might decrease the biological degradation effectiveness. In this publication, three series of tests were conducted to study possibilities to reduce these matters: bacterial adaptation which was conducted in a biological aerated filter, adsorption/biosorption conducted on a biological...

Author(s): Stenelvie Dajeavine NGALA, Ahmed SALIM, Sarah JERROUMI, Brahim LEKHLIF, and El Hassan MALIL  

Simple sequence repeat (SSR) marker-based DNA fingerprinting of some varieties of rice (Oryza sativa L.) released in Nigeria

March 2019

Over time, precise, specific and rapid identification of variety have been achieved via fingerprinting with molecular markers. In this research, 13 out of 45 screened simple sequence repeat (SSR) markers were employed to fingerprint 27 rice varieties (22 are commercially released while five are suspected duplicates from the hands of marketers with different names) in Nigeria. The SSR primer pairs (13) were polymorphic...

Author(s): Ishaq, M. N., Ehirim, B. O., Nwanyanwu, G. C. and Abubaka, R. I.  

Polymorphisms in IL-10 (-1082) and IFN-𝛾, (+874) cytokine genes associated with resistance or susceptibility to Schistosoma haematobium infection in primary school children of Mount Darwin, Zimbabwe

March 2019

The promoter region of human interleukin-10 (IL-10) gene is highly polymorphic while the first intronic region of interferon gamma (IFN-) gene is also highly polymorphic. These polymorphisms are associated with susceptibility or resistance to Schistosoma haematobium infection. Schistosomiasis is known to be a highly inflammatory disease that requires the delicate balance of pro- and anti-inflammatory cytokines. The...

Author(s): Tendai Makwikwi and Takafira Mduluza  

Physicochemical and microbiological stability of muffins packed in actives edible coatings from cassava starch: Inverted sugar/sucrose and natural additives

March 2019

The aim of this study was to develop edible coatings containing natural additives incorporated to the matrix of cassava starch, for use in muffins, as well as to evaluate the efficacy of its use in the increase of shelf life and maintenance of the products characteristics. Seventeen film forming formulations were developed following a central composite experimental design (CCD). The influence of independent variables...

Author(s): Luana de Oliveira Melo Naponucena, Bruna Aparecida Souza Machado, Luciana Emanuella Ferreira Saraiva, Samantha Serra Costa, Rejane Pina Dantas Silva, Emanuelle Andrade Dantas, Roseane Santos Oliveira and Janice Izabel Druzian  

Genetic diversity of antibiotic and plant extract resistant Staphylococcus aureus isolated from hospitalized patients in Ekiti State, Nigeria

March 2019

This study aimed to determine the genetic diversity of antibiotics and plants extract resistant Staphylococcus aureus using molecular technique. A total of 106 human clinical samples were collected from patients in three different hospitals within Ekiti State. Microbiological and molecular analyses were performed using standard methods. Antimicrobial susceptibility test with common antibiotics and plant leaves extracts...

Author(s): Egbebi Adeola Oluwagbemileke, Oyama Maslhanty Obem and Fadare Olalekan Shadrach  

Genetic diversity using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in Tinospora cordifolia from the Rayalseema region in Andhra Pradesh

March 2019

Tinospora cordifolia is a highly important medicinal plant in India, with a rich source of different secondary metabolites. In the present study, 12 samples of T. cordifolia (collected from different areas of Rayalseema region,Andhra Pradesh) were studied for genetic diversity using 15 random amplified polymorphic DNA (RAPD) and eight inter-simple sequence repeats (ISSR) markers. Out of them, 10 RAPD and 5 ISSR primers...

Author(s): H. Fathima Nazneen, B. Arunkumar Naik, P. Ramesh, A. Chandra Sekhar and P. Chandramati Shankar  

Improvement of nutritional, sanitary and organoleptic qualities of liquid zoom-koom and instant flour zoom-koom using Lactobacillus fermentum starter culture

February 2019

Traditional fermented beverages occupy an important place in the diet of populations in Burkina Faso. Zoom-koom in particular remains one of the most consumed cereal-based drinks in cities like Ouagadougou. The drink zoom-koom is consumed primarily in the liquid form and to short scale in the reconstituted form called instant flour zoom-koom. Being able to diversify zoom-koom taste and aroma and to improve the...

Author(s): Soma M., , Kaboré D., Tankoano A., Compaoré C. S., Parkouda C., Toguyeni A. and Sawadogo-Lingani H.

Stem bark of Zanthoxylum zanthoxyloïdes a possible substitute of root bark for the conservation of the species in Burkina Faso

February 2019

Zanthoxylum zanthoxyloïdes (Lam.) is used for some health purposes in Burkina Faso. However, human action threatened this species in its native environment and its regeneration is difficult by the way of population. The main focus of this study is to discriminate samples of zanthoxyloïdes from different plant populations in order to find good specimens for traditional medicine and thus contribute to the...

Author(s): Lassané Ouédraogo, Aminata P. Nacoulma, Moussa Compaoré, Latifou Lagnika and Martin Kiendrebeogo  

Analysis of the mitotic effect of Annona muricata leaf extracts on Allium cepa root tip cells

February 2019

There has been a growing interest in medicinal plants by the locals of the Pacific Islands since it is readily available. Annona muricata, commonly called soursop is one of such plants which is popular because it is being used to treat initial stages of cancer due to its antioxidant properties. This study investigated various extraction methods which could be used to compare and see the effectiveness of each on the...

Author(s): Rehana Bibi, Ketan Christi, Marta Ferreira, and Nwangburuka Cyril  

Quantitation of differential expression of transcription factors under moisture deficit stress in sugarcane

February 2019

Moisture deficit stress, one of the abiotic stresses, affects sugarcane growth and development and reduces cane and sugar yields. Transcription Factors (TFs) are master regulatory proteins in all living cells which have the capability of activating or repressing transcription of stress responsive genes in order to activate the stress tolerance mechanism. Study of expression profiles of TF genes which regulate the...

Author(s): Manel Dapanage, and Sumangala Bhat,  

Comparison of oil quality extracted from selected conventional and non conventional sources of vegetable oil from Malawi

February 2019

In this study, oil quality with respect to physicochemical and phytochemical characteristics extracted from nonconventional seed oil namely Moringa oleifera, Adansonia digitata, Parinari curatellifolia and Cajanus cajan and conventional seed oil namely soybean (Glycine max) and groundnut (Arachis hypogaea) was assessed and compared. Results showed that there were significant differences in various quality parameters...

Author(s): Lesten Eliez Chisomo Chatepa, Hankie Uluko and Kingsley Masamba  

Regulation of a dominant glutathione S-transferase and Na+/K+ by spermidine under salinity in onion

February 2019

Spermidine (Spd) is one of the most important polyamines (PAs) showing important roles in growth, development and stress responses in plants. The role of Spd in regulation of a dominant glutathione S-transferase (GST, E.C.2.5.1.18) and ionic balance of Na+ and K+ in onion leaves were examined. Onion GSTs were separated from fresh bulb tissue using DEAE-cellulose chromatography. Three GSTs were eluted at 56, 120 and 169...

Author(s): Tanjina Islam, Md. Rezwan Molla, Reshma Sultana, Md. Bazlur Rahman, Munshi Rashid Ahmad and Md. Motiar Rohman  

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

February 2019

Eighteen ISSR primers (between Simple Repeated Sequences) were used to estimate the genetic diversity of Angolan cassava populations in order to identify genetic variabilities and their potential to be used in the diversification of cassava crops in Angola. In order to do so, three populations from three Angolan provinces were analyzed (Cuanza Norte, Uige and Malanje), which totaled 40 genotypes. In total, 116 bands...

Author(s): Sandra Domingos João Afonso, Ricardo Franco Cunha Moreira, Carlos Alberto da Silva Ledo, Cláudia Fortes Ferreira, Vanderlei da Silva Santos and Pascoal António Muondo  

Antioxidant, cytotoxic, and antidiabetic activities of Dendropanax morbifera extract for production of health-oriented food materials

February 2019

Antioxidant, cytotoxic and anti-diabetic effects of fermented and non-fermented Dendropanax morbifera extracts were compared to assess the potential utility of this species in the development of health-oriented food. The non-fermented extract (NFDE) was obtained from leaves and branches of D. morbifera and the fermented extract (FDE) was prepared by inoculation with Lactobacillus plantarum and Lactobacillus brevis after...

Author(s): KiBeom Lee and Andy Kim  

Enhancement of saponin accumulation in adventitious root culture of Javanese ginseng (Talinum paniculatum Gaertn.) through methyl jasmonate and salicylic acid elicitation

February 2019

Javanese ginseng (Talinum paniculatum) is an herb and one of the traditional medicinal plants in Indonesia which accumulate saponin in the root organ. However, slow root growth hampers the accumulation of the compound in this plant. Therefore, in vitro culture of adventitious roots offers an alternative way to increase the production of saponin. Furthermore, this study was performed to investigate the effect of methyl...

Author(s): Ahmad Faizal and Anggita Veningtia Sari  

Obtainment, applications and future perspectives of palm kernel

January 2019

Of African origin and with an estimated world production of 6.85 million tons, palm kernel oil contributes about 3% of the total world production of oils and fats. With such production, the world waste generation was about 10,026 × 103 tons in 2017, which represents an important environment issue. The present work aimed to review the literature on the main methods of extraction, applications, and future...

Author(s): Wanessa Almeida da Costa, Cinthya Elen Pereira de Lima, Fernanda Wariss Figueiredo Bezerra, Mozaniel Santana de Oliveira, Priscila do Nascimento Bezerra, Flávia Cristina Seabra Pires, Ana Paula de Souza e Silva, Jorddy Neves da Cruz, Sebastião Gomes Silva, Pedro Alam de Araújo Sarges and Raul Nunes de Carvalho Junior,  

Antimicrobial activity of Basidiomycetes fungi isolated from a Kenyan tropical forest

January 2019

Microbial diseases remain to be a major global public health challenge. Their devastating effects have been exacerbated by the development of drug resistant strains. On the other hand, the biodiversity of tropical Basidiomycetes fungi is well recognized as an untapped source of potential bioactive compounds for the development of novel antimicrobials. Thus, in the current study, Basidiomycetes’ fruiting bodies...

Author(s): Winnie Chemutai Sum, Stephen Abwao Indieka and Josphat Clement Matasyoh

Effect of cutting position and indole butyric acid (auxin) concentration on rooting response of Araucaria heterophylla

January 2019

The effects of cutting position (tip, middle and basal) and concentration of indole butyric acid (IBA) (0, 5, 7.5 and 11 g/L) on root and shoot growth of Araucaria heterophylla were evaluated to develop a method for vegetative propagation for this tree species. Leaf number, number of adventitious roots, root length and survival rates were measured. These parameters were significantly influenced by the interactive effect...

Author(s): Abera Tilahun, Begashaw Manahlie, Getachew Abebe and Genet Negash  

In vitro regeneration of two grapevine (Vitis vinifera L.) varieties from leaf explants

January 2019

The traditional way of grapevine (Vitis vinifera L.) propagation is time consuming and allows disease transmission from generation to generation. Moreover, it is difficult to improve this crop through conventional plant breeding methods. Therefore, the objective of this study was to develop efficient in vitro regeneration protocol for ‘Canonannon’ and ‘Chenin Blanc’ varieties of grapevine using...

Author(s): Fikadu Kumsa and Tileye Feyissa  

Plant biotechnology: A key tool to improve crop production in Rwanda

January 2019

Rwanda’s economy relies on agriculture; nevertheless, crop production remains insufficient for both local consumption and exportation. Overall, enough agriculture yields in Rwanda to ensure food security has not yet been achieved regardless of more than 87% population engaged in agriculture activities. In this context, this study aimed at gathering the information on Rwanda’s agriculture based on different...

Author(s): Leonce Dusengemungu, Clement Igiraneza and Sonia Uwimbabazi  

Is the introgression of Lobi/Baoulé cattle by zebuine genes in Burkina Faso Lobi cattle threatened?

January 2019

Burkina Faso livestock is made up of two main cattle population, namely Zebuine and Taurine. Transhumance and settlement of Zebu cattle breeders in tsetse challenged areas lead to cross-breeding Zebu and Taurine. Introgression of the Zebu cattle may have changed the structure of the trypanotolerant Lobi/Baoulé breed. The objective of the present study was to appreciate the introgression of Zebu genes into...

Author(s): Albert Soudré, Moustapha Gréma, Stéphane A. R. Tapsoba, Amadou Traoré, Salifou Ouédraogo-Koné, Maria Wurzinger, Simone Müller, Olivier Hanotte, Anicet Georges Ouédraogo and Johann Sölkner  

Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

January 2019

Yellow rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat in Ethiopia. Poor knowledge of resistance genes deployed in Ethiopian wheat cultivars is one major factor for recurrent epidemics of rust diseases. Molecular marker based gene identification showed the presence of Yr1, Yr4, Yr6, Yr7, Yr9, Yr10, Yr17 and Yr18 in various frequencies, whereas Yr8 was not detected in...

Author(s): Woubit Dawit, Ayele Badebo, Bekele Hunde, Daniel Kassa and M. S. Röder  

Evaluation of the morphological and quality characteristics of new papaya hybrid lines in Kenya

January 2019

Papaya (Carica papaya L.) is among the most grown fruit crops worldwide with high economic and nutritional value. In Kenya, the papaya industry relies heavily on imported varieties and farmers’ selected seed whose quality is not known. Therefore, the morphological and quality characteristics of mature fruits of eight newly developed papaya hybrids and their control, Sunrise solo were assessed using papaya...

Author(s): Gaudence Nishimwe, Janet Chepng’etich Kosgei, Everlyn Musenya Okoth, George Ochieng’ Asudi, and Fredah Karambu Rimberia  

Decolourization of synthetic dyes by laccase enzyme produced by Kluyveromyces dobzhanskii DW1 and Pichia manshurica DW2

January 2019

Industrialization has come with environmental challenges. Industries like paper, printing, textile, leather and so on widely use chemical dyes whose waste treatment or degradability is difficult. Among various methods employed, the use of microbial enzymes is the most effective. The study aimed at producing laccase from identified yeast strains for potential industrial use in dye decolourization. Laccase produced by...

Author(s): Sherifah Monilola Wakil, Seun Andrew Eyiolawi, Kehinde Olamide Salawu and Abiodun Anthony Onilude  

Enhancement of anaerobic batch digestion of spineless cacti (Opuntia ficus indica) feedstock by aerobic pre-treatment

January 2019

One of the best options for African countries to meet rural energy needs is to grow care-free crassulacean acid metabolism plants on a massive scale in waste lands. This can enable bioenergy production without disrupting food supplies and hence sustainable energy supply for the future. Opuntia ficus indica is an ideal plant for arid regimes but has barely been studied as a potential bioenergy source. This study...

Author(s): Hawa Myovela, Anthony Manoni Mshandete and Samuel Imathiu  

Evaluation of the diversity in qualitative traits of Bambara groundnut germplasm (Vigna subterranea (L.) Verdc.) of Côte d’Ivoire

January 2019

The objectives of this study were to assess phenotypic diversity of Bambara groundnut germplasm from Côte d’Ivoire using qualitative traits and to understand the genetic diversity at different levels. Hundred and one accessions collected from four agro-ecological zones (central, eastern, northern, western) were characterized in a randomised complete block design with three replications. Thirteen qualitative...

Author(s): Beket Séverin BONNY, Dagou SEKA, Koffi ADJOUMANI, Kouamé Guillaume KOFFI, Léonie Clémence KOUONON and Raoul Sylvère SIE  

Degradation kinetics of 2,4-dichlorophenoxyacetic and atrazine by Trametes versicolor (L.:Fr.) Pilát

December 2018

The degradation kinetics of different sources of 2,4-dichlorophenoxyacetic (2,4-D) and atrazine by the natural strain Mo008 of the basidiomycete fungus Trametes versicolor (L.:Fr.) Pilát was studied, knowing that in this process the strain used produces an enzyme complex composed of manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase, and the response on the global consumption rate and yield is...

Author(s): Ernesto Hernández Mendieta, Dagoberto Guillén Sánchez, Iran Alia Tejacal, Victor López Martínez, María Andrade Rodríguez, Edgar Martínez Fernández, Manuel Huerta Lara, and Catalina Rubio Granados  

Isolation and characterization of Ralstonia solanacearum strains causing bacterial wilt of potato in Nakuru County of Kenya

December 2018

In Africa, cultivation of potato (Solanum tuberosum) represents an important food source and income generation. However, its productivity is constrained by biotic and abiotic stresses. Bacterial wilt caused by Ralstonia solanacearum is an important constraint to the world potato industry. In Kenya, the disease affects 77% of potato farms causing yield losses of up to 100%. Control methods are limited mainly due to the...

Author(s): Rostand Romeo Chamedjeu, Joel Masanga, Viviene Matiru, and Steven Runo  

Dynamics of the concentration of the hormones triiodothyronine and thyroxine at the beginning of the reproductive season and the effect of application of iodine in Nelore heifers bred in pasture

December 2018

The aim of this study was to evaluate the dynamics of triiodothyronine (T3) and thyroxine (T4) hormones, their relation with body condition score (BCS) and reproductive stage of 248 Nelore heifers, and the effect of iodine application on the induction of cyclicity of 217 anoestrus heifers in pasture at the beginning of the reproductive season. At day zero (D0), an assessment of the BCS was performed. An examination of...

Author(s): Adriana Almeida Fonseca Ruy, Karen Martins Leão, Mariana da Mata Silveira, Viler Carrijo Oliveira, Francisco Ribeiro de Araújo Neto and Marco Antônio Pereira da Silva

Comparison of antimicrobial properties of two spices commonly consumed in Nigeria and effect of temperature on their antioxidant properties

December 2018

The study compared the antimicrobial activities of essential oils (EOs) from Piper guineense (Uziza) and Xylopica aethiopica (Uda) using diffusion and dilution assays, and evaluated their antioxidant properties at different temperatures. Comparison of the antimicrobial activities was determined using well-in-agar diffusion, disc diffusion and broth dilution methods against Escherichia coli, Salmonella sp and Bacillus...

Author(s): Ogueke, Chika C., Azeke, Ehijie Augusta, Owuamanam, Clifford I., Ojukwu, Moses, Agunwah, I. M., Barber, L. I. and Bede, E. N.  

Effects of heat sources on the levels of polycyclic aromatic hydrocarbon in selected fish samples from Ogidingbe (Escravos Estuaries)

December 2018

The riverine areas of the Niger-Delta region of Nigeria are popularly known for their smoked fish from both fresh and salt water. To the people living in these areas, smoking is a method of preservation as the fish is known to undergo spoilage within 8 h of harvest from its natural habitat; though not without the potential health hazard associated with smoked foods. During smoking, polycyclic aromatic hydrocarbons...

Author(s): Erhabor Destiny, Edjere Oghenekohwiroro and Ejeomo Christopher

Clausenidin upregulated p53 and caused apoptosis in HT-29 tumor cell lines

December 2018

Clausenidin is a pyranocoumarin majorly found in medicinal herbs of the Rutaceae family used to treat cancer patients locally in Asia. The compound is presumed to have anti-cancer cell effect but its exact mechanism of action is still unknown. The study aimed to evaluate the effect of pure clausenidin on p53-mediated apoptosis as well as other cell death pathways in colon cancer (HT-29) cell lines. The...

Author(s): Peter M. Waziri, Rasedee Abdullah, Rosita Rosli, Abdul Rahman Omar, Ahmad Bustamam Abdul, Nur Kartinee Kassim, Ibrahim Malami, Imaobong C. Etti, Palanisamy Arulselvan and Ashwaq Shakir Al-Abboodi  

Quali-quantitative characterization of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira): macroscopic, microscopic, physico-chemical and microbiological parameters

December 2018

The objective of the present study was to evaluate the external and organoleptic characters, microscopic, physico-chemical, chromatic and microbiological parameters of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira) of the Jequitinhonha River Valley / Brazil, in order to create its geographical indication. In the first stage a data survey was carried out to characterize the production of...

Author(s): Eliane Macedo Sobrinho Santos, Hércules Otacílio Santos, Juliana Rezende Sá Miranda Gonçalves, Anna Christina Almeida, Igor Viana Brandi, Alexsander Rodrigues Cangussu, Janainne Nunes Alves, Ricardo Jardim Neiva, Grazielle Layanne Mendes Santos, Maria Izabel de Jesus Viana, Bruna Ruas Santos Araújo, Thalita Cordeiro Santos and Kattyanne Souza Costa

Determination of trace elements and heavy metals content of green and black tea varieties consumed in Iran

December 2018

The aim of the present study was to determine the level of selected heavy metals in green and black tea varieties consumed in Iran. Samples were prepared with the acid digestion method and measured by polarography. The mean concentration of Zn, Cd, Pb and Cu was 0.505±1.632, 0.417±0.268, 0.325±0.249 and 4.151±3.739 mg/100 g for black tea, and 2.442±4.003, 0.284±0.198,...

Author(s): Behrooz Jannat, Naficeh Sadeghi,, Mohammad Reza Oveisi, Masoomeh Behzad, Manan Hjimahmoodi and Morteza Ebrahimi  

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

December 2018

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated...

Author(s): Nguyen Thi Thu#, Vu Hong Quang#, Mai Van Tan#, Vu Đuc Lam, Nakano Toshitsugu, Nguyen Thi Hue, Nong Thi Hue, Nguyen Viet Long, Nguyen Van Hoan, Vu Van Liet, Nguyen Thi Mien, Tran Thi Lien, Nguyen Dinh Trung and Tran Thi Thu Hoai

Superoxide dismutase activity and jasmonic acid during in vitro-ex vitro transition of pineapple (Ananas comosus (L.) Merr.) micropropagated plantlets

December 2018

Recent agriculture is characterized by intensive and cleaning productions, which need seeds with high quality in large quantities bonded by in vitro culture labs. Nevertheless, in vitro ex vitro transition and during acclimatization losses occur due to the death of plantlets unable to survive this abiotic stress. Reactive oxygen species production during jasmonic acid-induced changes of previous transition was...

Author(s): González-Olmedo J. L., Garza-García Y., Mbogholi A., Rodríguez-Escriba R. C., Aragón C. E., Rodríguez R., and Moreno A.  

Preliminary study on rice straw degradation using microbial inoculant under shake flask condition

December 2018

Abundance and unmanaged agriculture residues lead to unutilized resource waste and environmental pollution. Application of microbial technology to manage agriculture waste could produce value-added product. A preliminary study on biodegradation of rice straw using different potential microorganisms was tested under controlled environment. Three different inoculant cultures were used to observe their efficiency in rice...

Author(s): Nurul Ain Abu Bakar, Mohammad Hariz Abdul Rahman, Nur Alyani Shakri, Syuhaidah Abu Bakar and Abhar Abdul Hamid

DNA extraction from silica gel-preserved common bean (Phaseolus vulgaris L.) leaves

December 2018

Extraction of non-degraded and contaminant-free DNA from field specimen requires collection under liquid nitrogen which is not readily available in resource constrained laboratories in low and middle income countries (LMICs). A method of extracting DNA from silica gel-preserved common bean (Proteus vulgaris L.) leaves is presented. The method, which does not involve the use of phenol, chloroform or isoamyl alcohol also...

Author(s): Male Allan Ssekamatte, Kato Fred and Mukankusi Clare Mugisha

Marker-assisted backcrossing for associating nutritional quality to drought tolerance in barley

November 2018

Backcross breeding enhanced by marker assisted selection is currently the most powerful method used to transfer one or a few genes controlling specific traits from one line into another. This method was used to cross naked barley variety, “Tombari” known as A, having high nutritional quality with hulled variety, “Giza131” called B, highly tolerant to drought. The objective of this work is to...

Author(s): Amani Ben Naceur, Sameh Mnasri, Hatem Cheikh-M’hamed, Chedly Abdelly and M’barek Ben Naceur  

Identification and mapping of quantitative trait loci associated with soybean rust (Phakopsora pachyrhizi) resistance in genotype UG 5

November 2018

Soybean rust, Phakopsora pachyrhizi, is one of the most serious and widespread foliar diseases of soybean causing high yield losses world-wide. The objective of this study was to identify and map quantitative trait loci (QTLs) resistant to soybean rust in genotype UG 5. Ninety-seven F2 mapping plants, obtained from a cross between Wondersoya and UG 5, were used for this study. Quantitative trait locus analysis using QTL...

Author(s): Hailay Mehari Gebremedhn, Ulemu Mercy Msiska, Miesho Belay Weldekidan, Fentaw Abate Asmamaw, Akech Winnifred, Dramadri Isaac Onziga, Thomas Lapaka Odong, Patrick Rubaihayo and Phinehas Tukamuhabwa

Characterization of fifteen microsatellite loci and genetic diversity analysis for the Ghanaian food security crop Solenostemon rotundifolius (Frafra potato)

November 2018

Frafra potato (Solenostemon rotundifolius) is an important food crop indigenous to Western Africa and a member of the Labiaceae (mint) family. It is nutrient-rich and drought tolerant; however, despite its potential as a food security crop, it has not received the research support needed to promote its widespread adoption and safeguard its diversity. As a result, Frafra potato is becoming extinct and restricted to...

Author(s): Lichun Hua, Đenita Hadziabdic, Naalamle Amissah, Marcin Nowicki, Sarah L. Boggess, Margaret Staton, Nianjun Teng and Robert N. Trigiano

Evaluation of genetic diversity of pearl millet (Pennisetum glaucum L.) landraces from West Africa using microsatellite markers

November 2018

Pearl millet (Pennisetum glaucum L.) is an important staple food crop, especially in arid and semi-arid regions of West Africa. However, the genetic diversity of pearl millet in these regions is poorly understood, and yet it is useful in identification of landmarks for in situ germplasm conservation and breeding programs, particularly in establishment of heterotic groups. Therefore, the aim of this study was to assess...

Author(s): Mary N. Onsarigo and Dionysious K. Kiambi  

Comparison of four DNA extraction methods in Heliconia (Heliconia L.)

November 2018

Molecular analysis in plants requires the use of high quality DNA. Currently, there are a myriad of DNA extraction protocols, which still seems that their applicability across different species is not straightforward. The aim of the present study was to evaluate four different cetyl trimethylammonium bromide (CTAB)-based DNA extraction protocols for Heliconia species from lyophilized fully mature leaves. A split-plot...

Author(s): Olvera S., Cortés-Cruz M., Contreras-Toledo A., Ortiz S., Avendaño-Arrazate C., Olivera A., Canul J. and Grajales M.  

Bioremediation potentials of sunflower and Pseudomonas species in soil contaminated with lead and zinc

October 2018

This study was conducted to assess the ability of sunflower (Tithonia diversifolia) inoculated with Pseudomonas species to remediate soils contaminated with heavy metals (lead and zinc). The contaminants were added to 20 kg soil in pots as lead nitrate (Pb (NO3)2) and zinc nitrate (Zn (NO3)2); 400 mg/l metal concentrations were added to 20 kg soil in pots. The experimental design was a randomized complete block design...

Author(s): Elizabeth Temitope Alori, Abiodun Joseph, Ojo Timothy Vincent Adebiyi, Ajibola Patrick Aluko and Chidera Onyekankeya  

Applications of genomic copy number variations on livestock: A review

October 2018

Genomic copy number variations (CNVs) have significantly modified genomic regions during inversions, translocations, duplications and deletions that keep shaping phenotypic variation through changes in gene dosage, regulation and transcript structure. The objective of this review was to discuss the current application of CNVs on domestication, adaptations, productions, coat colour, morphology and diseases of livestock,...

Author(s): Habtamu Abera Goshu, Min Chu and Ping Yan

Phenotypic and genotypic characteristics of cowpea rhizobia from soils of Ethiopia

October 2018

Phenotypic and genotypic characteristics of cowpea rhizobia indigenous to soils of Ethiopia are unknown. Forty indigenous cowpea rhizobial isolates were collected from cowpea-growing areas of the country and were characterized for their growth and genetic properties. Based on their cultural characteristics, the isolates were categorized into fast (FG), slow (SG), and extraslow-growings (ESG). The FG, SG, and ESG...

Author(s): Girmaye Kenasa, Fassil Assefa and Mussie Y. Habteselassie  

Propolis as natural additive: A systematic review

October 2018

Propolis extract is a resin collected by the bees and contains numerous active substances with a recognized pharmaceutical potential use. The aim of this study was to conduct a systematic review of studies with the use of propolis as a food additive and the microencapsulation technology for incorporation of the product in food. Studies in Portuguese, English and Spanish were identified from online databases, Lilacs,...

Author(s): Marly Silveira Santos, Maria Leticia Miranda Fernandes Estevinho, Carlos Alfredo Lopes de Carvalho, Karina Teixeira Magalhães-Guedes, Rosane Freitas Schwan and Rogeria Comastri de Castro Almeida

Anti-diabetic and some haematological effects of aqueous and ethanol leaf extract of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats

October 2018

This study investigates the anti-diabetic and some hematological effects of aqueous and ethanol leaf extracts of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats. Forty-eight albino mice were used for the lethal dose (LD50) study, while twenty-eight Wistar rats were used for the diabetic and haematological study. The result of the phytochemical analysis shows some important phytochemicals that could confer...

Author(s): Nduka, Florence O., Ogugua, Victor N., Joshua, Packer E., Okpachi, Victor E., Gometi, Sandra A. and Nwigwe, Juliet O.  

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

October 2018

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the...

Author(s): MADJITOLOUM BETOLOUM Salomon, TALLA Emmanuel, NYEMB Jean Noël, NGASSOUM Martin Benoît, TSATSOP TSAGUE Roli Karole and MAHMOUT Yaya  

Resveratrol inhibits cell viability and proliferation via apoptotic pathways in ovarian cancer

October 2018

Ovarian cancer is the most common female malignancy and the main cause of death from gynecological malignancies. Resveratrol (3,4,5 tri-hydroxystilbene) is a phytoalexin and a polyphenolic compound present in human dietary material such as peanuts, mulberries, grapes and red wine. We demonstrated that resveratrol depressed the proliferation of HO8910PM cells in a dose-dependent and time-dependent manner in vitro (P <...

Author(s): YAO Jing-ping, XU Fei, LI Rong, LI Meng and QU Jun-wei  

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