African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12370

AJB Articles

In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

December 2019

Regeneration of the coconut (Cocos nucifera L) through indirect somatic embryogenesis using 9 to 12 months embryos explants was established in Y3 medium supplemented with 100 to 250 µM 2,4 -dichlorophenoxyacetic acid (2, 4-D) alone and in combination with gibberellic acid (GA3), 6-benzylaminopurine (BAP) and thiadiazuron (TDZ). The highest callus induction (100%) was observed in medium containing 150 and 250...

Author(s): Caren C. Bett, Cecilia M. Mweu and Aggrey B. Nyende  

Effect of ethanolic leaf extract of Vinca major L. on biochemical parameters and glucose level of alloxan induced diabetic rats

December 2019

In view of World Health Organization (WHO) recommendation on the use of medicinal plants for the management of diabetes mellitus, this study evaluated the effect of ethanolic leaf extract of Vinca major L. on biochemical parameters and glucose level of alloxan induced diabetic rats as objective. Sixty-four male Wistar albino rats were induced with diabetes by a single intraperitoneal injection and were separated into...

Author(s): Monago-Ighorodje Comfort, Duru Majesty, Adindu Eze, Nwauche Kelechi, Ezekwe Ahamefula, Nosiri Ijeoma, Odika Prince, Onyeabo Chimaraoke, Ogar Ishade, Berezi Ebitimi Peter, Ugoh Austin Ikenna, Eboagwu Ijeoma and Otta Emmanuel  

Development of a rice yellow mottle virus amplicon biotechnological tool for the production of high value proteins in rice

December 2019

Lately, advances on biotechnology have led to the emergence of plants as bioreactor for the production of molecules of industrial, pharmaceutical and agribusiness interests. One of the major advances was the exploitation of plant viruses as “amplifier” for the protein production. This amplicon technology relies on viral vector engineered to contain a gene encoding candidate protein that is produced in...

Author(s): Pingdwende Kader Aziz Bamogo,, Christophe Brugidou, Fidèle Tiendrébéogo, Drissa Sérémé, Florencia Wendkuuni Djigma, Jacques Simpore and Séverine Lacombe,

An alternative caprine infection model for Mycoplasma mycoides

December 2019

Ruminant infection models for Mycoplasma mycoides are invasive and laborious factors that may interfere with the experiment outcomes. This brief review presents an alternative method that may minimize on the mentioned challenges earlier mentioned. M. mycoides subsp. capri GM12 and M. mycoides subsp. capri YCP1.1 ∆68 strains were used; the former, causing a septicemia in goats. An intra-tracheal inoculation technique...

Author(s): Ssajjakambwe Paul, Elise Schieck, Anne Liljander, Tweyongyere Robert, Matovu Enock, Kazibwe Ann, Nanteza Ann and Mwiine Frank

Phenotypic and molecular characterization of extended spectrum β-lactamase producing Pseudomonas aeruginosa in Nigeria

December 2019

This present study was undertaken for detection of extended spectrum β-lactamases (ESBLS) enzyme genes among clinical isolates of Pseudomonas aeruginosa using phenotypic and molecular techniques. Thirty-four P. aeruginosa isolates from different hospitals in Nsukka and University of Nigeria Teaching Hospital (UNTH), Enugu were screened for the presence of ESBL-encoding genes. Phenotypic screening for ESBL producers...

Author(s): Martina C. Agbo, Ifeoma M. Ezeonu, Maurice N. Odo, Chukwuebuka  M. Ononugbo, Beatrice O. Onodagu, Chinelo C. Eze, Ezinwanne  N. Ezeibe and Chizoba A. Ozioko  

First report of bovine papillomavirus genotypes in cutaneous lesions by polymerase chain reaction (PCR) and direct sequencing in Panama

December 2019

Bovine papillomavirus (BPV) causes benign tumours in the mucosal and cutaneous epithelium and is characterized by the presence of warts. The present study includes the molecular identification of BPV strains in samples of warts using degenerate polymerase chain reaction (PCR) primers FAP59/64. Skin biopsies were taken from crossbred cattle from two experimental farms of Instituto de Investigación Agropecuaria de...

Author(s): Rita González, Manuel Murillo, Hilda Elena Castillo M., Marcelino Jaén  and Axel Villalobos-Cortés

Survey of the incidence and distribution of groundnut rosette disease in major groundnut-producing regions of Western Kenya

December 2019

Groundnut rosette disease (GRD) is the most important viral disease of groundnuts in sub-Saharan Africa. In Kenya, GRD infection especially before flowering results in 100% loss in pod yield. Surveys were conducted in 2016 and 2017 to determine the incidence and distribution of GRD in five major groundnut growing Counties of western Kenya. A structured questionnaire was used to assess GRD incidence and severity and...

Author(s): Eric O. Were, Evans N. Nyaboga and Elijah M. Ateka  

Evaluation of Lactobacillus spp. isolated from locally consumed probiotic food in Nsukka, Enugu State, Nigeria for antimicrobial activity utilizing agar well diffusion and pH tolerance tests

December 2019

Antimicrobial activity against entero-pathogens and tolerance to acid stress are crucial characters of probiotic bacteria. Lactobacillus spp. isolates were phenotypically characterized using colony observation, catalase test and Gram stain reaction.  The pH (1.5, 2.5 and 3.5) tolerance of each isolate was evaluated at 0 and 4 h. The antibacterial activities of the isolates were tested against pathogenic strains of...

Author(s): Dinebari P. Berebon, Kenneth C. Ofokansi, Anthony A. Attama, Christopher O. Eze, Restus C. Onwusoba, Chukwudi T. Ugwu, Charity C. Eze and Somtochukwu A. Evurani  

Callus induction in three mosaic disease resistant cassava cultivars in Benin and genetic stability of the induced calli using simple sequence repeat (SSR) and sequence-characterized amplified region (SCAR) markers

November 2019

The effect of different concentrations of thidiazuron (TDZ), benzyl amino purine (BAP), kinetin and 2,4-dichlorophenoxy acetic acid (2, 4-D) on callus induction in three elite cassava cultivars (agric-rouge, atinwewe and agblehoundo) was evaluated. Leaf explants harvested from greenhouse-grown cassava were sterilised using different concentrations of commercial bleach commonly called Jik (3.85% NaOCl) at different time...

Author(s): Amitchihoué Franck Sessou, Jane W. Kahia, Elijah Ateka, Jerome Anani Houngue, Colombe Dadjo, Peter Njenga and Corneille Ahanhanzo  

Whole-genome optical mapping: Improving assembly of Macrophomina phaseolina MS6 through spanning of twelve blunt end chromosomes by obviating all errors and misassembles

November 2019

Deciphering genetic information through next-generation sequencing (NGS) is considered as the basic platform to unveil in details of an organism. However, as it produces short reads that lead to difficulties in assembly, we generated long scaffold-based optical mapping (OM) data of previously sequenced devastating fungus, Macrophomina phaseolina MS6. In the process, KpnI identified as the most effective restriction...

Author(s): Quazi Md. Mosaddeque Hossen, Md. Shahidul Islam, Emdadul Mannan Emdad, Md. Samiul Haque, Md. Monjurul Alam and Maqsudul Alam,

Evaluation of aflatoxins levels and molecular identification of toxigenic molds in cereals and cereal-derived breakfast foods in Nigeria

November 2019

In the last decade, there have been an increase in safety concerns on the aflatoxins contents of cereals and cereal-derived food products in Nigeria. In the current study, evaluation of aflatoxins levels and molecular identification of toxigenic molds recovered from cereals and cereal-derived foods products in Nigeria were studied. Enzyme Link Immunosorbent Assay (ELISA) and high performance liquid chromatography (HPLC)...

Author(s): Michael O. Odo, Fidelis Azi, Ignatius C. Alaka and Veronica N. Nwobasi

Relationship between 2-acetyl-1-pyrroline and aroma in Uganda rice populations with Oryza (barthi, glaberrima and sativa) backgrounds

November 2019

The sweet popcorn aroma conferred by 2-acetyl-1-pyrroline (2AP) is a highly economic trait of rice grain attracting premium price worldwide. This research study was conducted to determine the levels of 2AP in Ugandan rice lines with the aim of establishing a better understanding on the level and classes of 2AP and aroma phenotype. Concentration of 2AP was assayed using two-dimensional gas chromatography-time-of-flight...

Author(s): David Ocan, Rongrong Zhang, Martin Odoch, Michael Kanaabi,  Angele Pembele Ibanda, Agnes Akwero, Bill Williams Khizzah, Ephraim Nuwamanya, Jimmy Lamo, Melissa Anne Fitzgerald, Venea Dara Daygon and Patrick Rutimbanzigu Rubaihayo

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

November 2019

Coffea arabica, F1 hybrid variety, Ruiru 11 is a highly sought after crop in Kenya due to its alleviated resistance to Coffee Berry Disease and Coffee Leaf Rust coupled with high yield capacity and good cup quality. Access to the variety’s planting materials is limited due to challenges with difficulty in propagation using conventional methods of seed and vegetative propagation; and somatic embryogenesis is...

Author(s): Mwaniki W. I., Lubabali A. H., Asava K. K., Agwanda C. O. and Anami S. E.  

In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

November 2019

Jatropha curcas L. is among important tree crops in the world with a potential for biofuel production. In Ethiopia, there is a soaring investors’ interest to produce Jatropha in the country for biodiesel production. However, insufficient good quality propagation material is a major production constraint. A study was undertaken to establish a protocol for in vitro mass propagation of three Ethiopian Jatropha...

Author(s): Hundessa Fufa, Meseret Tesema and Jiregna Daksa  

Expression profiling, phylogenetic, and structural analyses of a laccase gene from the red palm weevil, Rhynchophorus ferrugineus

November 2019

Laccases, member of multicopper oxidase (MCO) family enzymes, play crucial roles in insects’ cuticle tanning and pigmentation. The purpose of this study was to identify and characterize a laccase gene from the red palm weevil (RPW), Rhynchophorus ferrugineus. The isolated RPW laccase gene sequence was 3,389 bp, including a 2,163 bp open reading frame that encodes 720 amino acids. The RPW laccase gene conserved the...

Author(s): Babiker M. A. Abdel-Banat and Hamadttu A. F. El-Shafie

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

October 2019

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s): Kedra Mohammed Ousmael, Kassahun Tesfaye, and Teklehaimanot Hailesilassie

Comparison of embryo developmental rates in Nguni, Bonsmara and Boran beef cattle breeds following in vitro fertilization and artificial insemination

October 2019

The aim of this study was to compare the embryonic developmental rates in the Southern African cattle breeds. To do this, cryopreserved semen straws from Nguni, Bonsmara, and Boran bulls were thawed at 38°C and evaluated for sperm motility characteristics using Sperm Class Analyser (SCA). The fertilizing ability of frozen/thawed sperm was evaluated by performing artificial insemination (AI) and in vitro...

Author(s): Mohleko Helen Mapeka, Cyril Mpho Pilane, Robert. Treadwell, Jones Ng’ambi and Cuthbert Banga  

Scientific applications and prospects of nanomaterials: A multidisciplinary review

October 2019

Nanotechnology is the science of objects <100 nm in size. Research into the development and application of nanomaterials takes a material science and engineering-based approach to nanotechnology. Nanomaterials often possess interesting optical, electronic and mechanical properties. The capacity to construct large, intricate structures with nanometer precision is rapidly increasing and consists several top-down...

Author(s): ADEOLA Adedapo Oluwasanu, FAPOHUNDA Oluwaseun, JIMOH Adeleke Teslim, TOLUWALOJU Tunde Isaiah, IGE Ayokunle Olalekan and OGUNYELE Abimbola Chris  

Economic viability of the biogas produced on pig farms in Brazil for electric power generation

October 2019

Electric energy generated by clean and renewable sources, such as biogas, is a subject widely discussed and of global importance. Biogas, besides being an alternative to other fuels uses a raw material, which in many cases is considered disposable, worthless and harmful to the environment when not correctly disposed of. Several factors influence the design, operation and collection of the final product. This paper is a...

Author(s): Levi Mariano Neto, Wellington Henrique Ponciano and Antonio Manoel Batista da Silva

Characterization of three full Iris yellow spot virus genes of a garlic-infecting isolate from Zimbabwe using next-generation sequencing technology

October 2019

Iris yellow spot virus (IYSV) is an important pathogen of Allium species worldwide. It has a tripartite genome consisting of the large (L), medium (M) and small (S) RNA segments. Despite its worldwide distribution, very few complete gene and genome sequences are available in public databases. The aim of this study was to obtain full gene sequences of a garlic-infecting IYSV isolate by next-generation sequencing (NGS)...

Author(s): Charles Karavina, Jacques Davy Ibaba and Augustine Gubba  

Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

October 2019

Alternanthera brasiliana is popularly known in Brazil as "penicillin" or "benzetacil" and is used in traditional medicine for the treatment of infections and for the healing of wounds. It is also used as an ornamental plant due to the characteristic purple coloration of its leaves when cultivated in shade. The objective of this study was to develop and validate an analytical methodology by...

Author(s): José M. T. de Alencar Filho, Hyany A. P. Teixeira, Iure S. de Carvalho, Milenna V. V. de O. Alencar, Nathália A. C. de Souza, Tarcísio C. de L. Arraújo, Emanuella C. V. Pereira, Isabela A. Amariz, Pedrita A. Sampaio, Pedro J. Rolim-Neto, Larissa A. Rolim, and Edigênia C. da C. Araújo,  

Variable DNA methylation in Ensete (Ensete ventricosum) clones associated with developmental stage revealed by Amplified Fragment Length Polymorphisms (AFLPs) with Methylation-Sensitive enzyme

October 2019

Ensete (Ensete ventricosum) is an important perennial species in Ethiopia. It is used for food and fiber. The mode of propagation is asexual and sexual. Asexual form of reproduction is by natural and anthropogenic induced sucker. This research aims to investigate and measure the methylation diversity of natural and anthropogenic induced sucker forming Ensete. Twenty-seven individuals from Hawassa University research...

Author(s): Hewan Demissie Degu and Bizuayehu Tesfaye  

Nutritional enhancement of cocoa pod husk meal through fermentation using Rhizopus stolonifer

October 2019

This study evaluated the effect of fermentation period on the proximate composition, anti-nutritional content, fibre fractions and amino acid profile of cocoa pod husk meal (CPHM). Cocoa pod husk was taken through a solid state fermentation process involving Rhizopus stolonifer as its starter culture for a period of two weeks. The fermented CPHM was dried and analyzed for its proximate composition, anti-nutritional...

Author(s): Olugosi O. A., Agbede J. O., Adebayo I. A., Onibi G. E. and Ayeni O. A.  

Optimizing DNA isolation protocol for rosemary (Rosemarinus officinalis L) accessions

October 2019

Rosemary plant is in high demand due to its application in traditional health care, food flavoring, fragrance and pharmaceutical industries. It contains high level of secondary metabolites which are responsible for its beneficial activities. Application of molecular techniques would facilitate the production of these substances and screening of accessions. The isolation of polymerase chain reaction (PCR) amplifiable...

Author(s): Zewdinesh Damtew Zigene, Bizuayehu Tesfaye Asfaw and Tesfaye Disasa Bitima  

Selection of Lactobacillus strains newly isolated from Algerian camel and mare fermented milk for their in vitro probiotic and lipolytic potentials

October 2019

The main objective of this study was the characterization of new lactobacilli probiotic strains belonging to lactic acid bacteria (LAB). Eighty-eight strains were isolated from different Algerian camel and mare fermented milks; three of them were pre-selected for their stability, fast growth and resistance to acidity and bile salts. Cell viability was assessed in simulated gastric and intestinal conditions. On the other...

Author(s): Sabrina Amara, Halima Zadi-Karam and Nour-Eddine Karam

Potential of Opuntia stricta Haw (Mexican elephant ear) in removing cyanobacteria in surface water

September 2019

In this study, assays were carried out to evaluate the efficiency of cactus Opuntia stricta cladodes as a coagulant for removal of cyanobacteria cells. To carry out the coagulation assays, water from eutrophic reservoir Bodocongó, in Brazilian semiarid, with cyanobacteria bloom of Microcystis aeroginosa, Plankthotrix isothrix and Cylindrospermopsis raciborskii were sampled. This water was submitted to coagulation...

Author(s): Janiele F. Nery, Gleydson K. M. Nery, Adriana G. Magalhães and Salomão S. Medeiros  

Phenotypic characterization of selected Kenyan Khat (Catha edulis) cultivars based on morphological traits

September 2019

Khat (Catha edulis Vahl) is an evergreen shrub habitually ingested for its euphoric and stimulatory effects. The crop is grown in the Middle East, Somalia, East Africa and Ethiopia. It is referred to as Miraa in Kenya, while in Yemen it has several names like Qat, Kat, Kath, Gat, Chat and Tschat. It belongs to the sub-order Rosidae, family Celastraceae and characterized by astringent taste. Despite the daily use and...

Author(s): Loise N. Ngari, Shadrack N. Njagi, James K. Kamau, Rose C. Lagat, Amos M. Musyoki, Beryl O. Akoth, Festus Kioko, Zedekiah-A Okwany and Mathew P. Ngugi  

Characterization of products from sawdust biodegradation using selected microbial culture isolated from it

September 2019

Characterization of products from sawdust degradation using selected microbial culture isolated from it was carried out. A composite sawdust waste sample was collected from MCC timber market in Calabar. The sample was diluted using serial dilution method and inoculated on Nutrient agar and Sabauraud dextrose agar using pour plate technique and incubated at 370C and 270C respectively. Five bacteria species and 3 species...

Author(s): Lennox, Josiah A., Asitok, A., John, Godwin E. and Etim, Blessing T.

Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties

September 2019

Three late blight resistance (R) genes from wild potato species were transferred as a stack into the farmer-preferred varieties ‘Tigoni’ and ‘Shangi’. Transgenic events were tested by detached-leaf assays (DLA) and whole-plant assays (WPA) with isolates of Phytophthora infestans using 20,000 sporangia / mL inoculum. For the first DLA, 9 out of 13 ‘Tigoni’ and 10 out of 12...

Author(s): Ethel N. Webi, Kariuki Daniel, Kinyua Johnson, Njoroge Anne, Ghislain Marc and Magembe Eric  

Aqueous Extracts of Pericopsis angolensis and Swartzia madagascariensis with High Antimicrobial Activities against Escherichia coli O157, Shigella spp. and Salmonella enterica subsp. enterica (Serovar typhi)

September 2019

This study determined the potential antidiarrhoeal potential of Pericopsis angolensis and Swartzia madagascariensis extracts against Escherichia coli O157, Shigella species and Salmonella Typhi. Extracts were obtained using the following methods: (i) hot water extraction (90°C) (LHWE), (ii) cold water extraction (CWED) and (iii) ethanolic extraction (EED). Antimicrobial effects of the extracts were determined using...

Author(s): Chingwaru Constance, Tanja Bagar and Chingwaru Walter,

Recombination fraction and genetic linkage among key disease resistance genes (Co-42/Phg-2 and Co-5/“P.ult”) in common bean

September 2019

Anthracnose (Colletotrichum lindemuthianum), Angular leaf spot (Pseudocercospora griseola) and Pythium root rot are important pathogens affecting common bean production in the tropics. A promising strategy to manage these diseases consists of combining several resistance (R) genes into one cultivar. The aim of the study was to determine genetic linkage between gene pairs, Co-42/Phg-2, on bean-chromosome Pv08 and...

Author(s): Dennis Okii, Arfang Badji , Thomas Odong, Herbert Talwana, Phinehas Tukamuhabwa, Allan Male, Clare Mukankusi and Paul Gepts  

Biopreservation of meat using the essential oil from Hyptis suaveolens Poit. (Lamiaceae) in Burkina Faso

September 2019

Essential oils are natural substances which can be used as natural conservators for foods. The present work aimed to evaluate biological properties of essential oil of Hyptis suaveolens and its effect on shelf-life of beef. The extraction was carried out by hydro-distillation. Antioxidant activity was determined by method of DPPH and FRAP. Antimicrobial activity was determined by the microdilution and agar diffusion...

Author(s): Henriette B. MIHIN, Marius K. SOMDA, Donatien KABORE, Souleymane SANON, Agbémébia Y. AKAKPO, Zenabou SEMDE, Alfred S.TRAORE and Aboubakar S. OUATTARA  

Biotechnological tools for detection, identification and management of plant diseases

September 2019

Detection and identification of plant pathogens is one of the most important strategies for sustainable plant diseases management. For this reason, the availability of fast, sensitive and accurate methods for detection and identification of plant pathogens is increasingly necessary to improve disease control decision making process. In other words, new technologies and improved methods with reduced/fair cost and...

Author(s): Tibebu Belete and Nuh Boyraz  

Cytogenetic and molecular assessment of some nanoparticles using Allium sativum assay

September 2019

One of the primary objectives in agriculture is providing high-quality crops to consumers. Multiple techniques and methods are utilized to achieve this objective, including nanotechnology that depends on the use of very small materials, which will help in decreasing the amounts usually used with similar effects. Nanomaterials are used as fertilizers and also as component of nano-pesticides for plants. Despite their...

Author(s): Mona S. Al-Ahmadi  

Phylogenetic analysis of bluetongue virus serotype16 based on genome segment 5 (encoding NS1)

September 2019

Bluetongue virus (BTV) is the ‘type’ species of the genus Orbivirus within the family Reoviridae. The BTV genome is composed of ten linear segments of double-stranded RNA (dsRNA), each of which codes for at least one of ten distinct viral proteins. Phylogenetic analysis based on VP2, VP3, VP5, VP7 and NS3 gene has been advocated by different researchers around the world. However, very little information...

Author(s): Molalegne Bitew, Sukdeb Nandi, Chintu Ravishankar and Asit Sharma

Molecular identification of acetic acid bacteria isolated from fermented mango juices of Burkina Faso: 16S rRNA gene sequencing

September 2019

Acetic acid bacteria are known for their ability to oxidize ethanol to acetic acid. This study investigated to identify dominant strain acetic acid bacteria involving in fermented juice of mango and capable to produce Vinegar, total of 4 bacteria (CRSBAN-BVA1, CRSBAN-BVK1, CRSBAN-BVK2, CRSBAN-BVI1) bacteria strains were preselected ¶for the analyses. The strains were examined with biochemical, physiological and...

Author(s): Assiètta OUATTARA, K. Marius SOMDA, A. T. Cheik OUATTARA, Bassirou N’DOYE, S. Alfred TRAORE and S. Aboubakar OUATTARA  

Studies on the sod culture and the management of soil moisture for the improvement of waxapple quality

August 2019

In Taiwan, Kaohsiung and Pingtung districts are the main cultivating areas of waxapple in Taiwan. The quality of waxapple is commonly involved in appropriate fertilization. Therefore, the demonstration orchards were established in Kaohsiung and Pingtung districts. This research was conducted to the effects of sod culture and management of soil moisture on the improvement of waxapple quality. The results showed that soil...

Author(s): Yong-Hong Lin and Juei-Yu Chiu

The urothelium enhancive polarization in CfTX-1 peptide intervened toad urinary bladder

August 2019

Toad urothelium barrier is the model to mimic and investigate urothelium permeability. Thiazide blocked ionic transportation in polarized membrane state. Jellyfish venom causes pores to be adjusted to urothelium permeability which improved polarization. This study aimed at CfTX-1 peptide in urothelium permeability evoked polarizations. Thiazide pretreated toad urothelium permeability to ions were investigated in...

Author(s): Ziduo Shen#, Xunguo Yang,#, Joseph Akparibila Azure,#, Annie Christel Bell,, Yueyi Sun, Xinyi Cheng, Fiona Masesi Dhlamini, Lingfeng Gao, and Yang Wang,  

Effects of salt stress on antioxidant system activity and peroxidation damage in root tip cells of strawberry

August 2019

In this study, strawberry seedlings were treated with 250 mmol·L-1 NaCl solution; then the O2- production rate, the content of H2O2 and thiobarbituric acid reactive substance (TBARS), electrolyte leakage (EL), the enzyme activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the root tip cells were determined to be used for analysis after salt stress. The results revealed that the O2-...

Author(s): Ji-Zhong Wang, Cong Jin, Yun-Peng Wang, Bo-Qing Chen and Ji-Zhong Wang

Effect of explant source and different hormonal combinations on in vitro regeneration of Heracleum candicans Wall: An important medicinal herb

August 2019

In vitro regeneration of plants is influenced to a great extent by genotype, the type of explant used, media composition and plant growth regulators. This research was devised to study the effect of explant source and different hormonal combinations on the in vitro regeneration of Heracleum candicans. Two different explants viz; leaf and petiole were used to investigate their morphogenic response on MS medium...

Author(s): Mahroofa Jan, Seema Singh, Farhana Maqbool and Irshad Ahmad Nawchoo

Anti-Xanthomonas activity of Antarctic fungi crude extracts

August 2019

Agriculture suffers considerable losses in its production caused by plant diseases. In the citrus culture some problems were associated with citrus canker caused by Xanthomonas citri subsp. citri. That same way, sugarcane plantations are impaired to leaf scald disease caused by Xanthomonas albilineans. One of the measures used to contain citrus canker is the spray of cupric bactericides; however for scalding leaves...

Author(s): Juliano Henrique Ferrarezi, Juliana Aparecida dos Santos, Lara Durães Sette, Henrique Ferreira and Daiane Cristina Sass

Morphological and molecular characterization of Jatropha curcas L. germplasm in Botswana

August 2019

Jatropha curcas L. is an undomesticated plant species that has recently received great attention for its utilization in biofuel production in many countries including Botswana. The experiment investigated 30 J. curcas accessions, and the results revealed high significant differences (P<0.01) among accessions for peduncle length, number of seed per fruit, length of fruit stalk, fruit length, fruit width, seed length,...

Author(s): C. Gwafila, U. Batlang and S. Ngwako  

Antioxidants enhance banana embryogenic cell competence to Agrobacterium mediated transformation

August 2019

Gene transfer into the plant cell is a key step towards its successful genetic modification and its efficiency is heavily dependent on plant and bacterial cell biological status and a wide array of physical conditions. Gene transfer efficiencies in East African Highland Banana (EAHB) cell lines remains low compared to other monocotyledonous crops like rice and wheat due to factors such as high oxidative stress. The use...

Author(s): B. Namukwaya, B. Magambo, G. Arinaitwe, C. Oweitu, R. Erima, D. Tarengera, D. L. Bossa, G. Karamura, A. Muwonge, J. Kubiriba and W. Tushemereirwe

Preparation and physicochemical characteristics of cross-linked resistant starch under heat-moisture treatment

August 2019

Sweet potato starch was subjected to cross-linking to achieve high content of resistant starch (RS) under heat-moisture treatment and the physicochemical properties of cross-linked sweet potato starches were investigated. The cross-linking agent was sodium trimetaphosphate and sodium tripolyphosphate (STMP/STPP). A maximum RS content (72.45%) was obtained after 10% STMP/STPP (99/1% w/w) at 120°C and pH 11.5...

Author(s): Meifang Cao, Yunyun Li and Qunyu Gao

Comparative study of fatty acid composition and nervonic acid contents of four tropicals plants: Ricinodendron heudelotii, Cyperus esculentus, Citrullus colocynthis and Irvingia gabonensis from Côte d’Ivoire

August 2019

This work was carried out to compare the potential applications of Ricinodendron heudelotii, Cyperus esculentus, Citrullus colocynthis and Irvingia gabonensis oil seeds by investigating their physicochemical parameters. Physicochemical parameters of the extracted oils were respectively as follow: Refractive index (1.49; 1.46; 1.45 and 1.44), acid value (1.35±0.02; 1.87 ± 0.45; 13.85 ± 0.02...

Author(s): DIOMANDE Issaka, DJOHAN Ferdinan, SORO Peforo, KOFFI Camille, KAPONGO K. Bertin and TIAHOU G. Georges  

Review of microfluidics approaches to mimic the kidney

July 2019

The use of microfluidics and nanotechnology in bioscience is gaining favor among the next generation of bioscience and clinical researchers, and this field has expanded to include organ-specific applications and modeling. While certain organ-specific microfluidic chips/applications, such as those in the heart and liver, are ubiquitous in the peer-reviewed literature, the kidneys require more focus and attention to drive...

Author(s): Zach Odeh, and Hongli Lin,

Molecular characterization of some bacteria isolated from munitions contaminated sites in Kachia Military Firing Range, Kaduna State, Nigeria

July 2019

Environmental pollution is principally caused by human activities that usually result in the release of man–made pollutants such as biological, chemical and radioactive in such states as solid, liquid and gaseous substances into the biosphere via, land, water and air. Globally, the increasing rate of environmental contamination by ammunition/explosives resulting from their increasing deployments in the rising...

Author(s): A. I. Alhaji, B. C. Onusiriuka, D. B. Maikaje, J. A. Appah, P. A. Vantsawa, Y. Magaji, A. A. Haroun, E. E. Oaikhena and E. O. Oladapo

Enhancement of phytoremediation efficiency of Acacia mangium using earthworms in metal-contaminated soil in Bonoua, Ivory Coast

July 2019

In this paper, the impact of Pontoscolex corethrurus on Acacia mangium growth and its phytoremediation efficiency in metal-contaminated soils were studied in a greenhouse pot culture under four treatments: non contaminated soil-non inoculated, control (C); non contaminated soil-inoculated (NcI); contaminated soil-non inoculated (CNi); contaminated soil-inoculated (CI). The results showed that A. mangium growth...

Author(s): BONGOUA-DEVISME Affi Jeanne, AKOTTO Odi Faustin, GUETY Thierry, KOUAKOU Sainte Adélaïde Ahya Edith, NDOYE Fatou and DIOUF Diégane  

Morphological and molecular screening of rice accessions for salt tolerance

July 2019

The ever-increasing demand for rice raises the need to increase productivity by using salt tolerant varieties on saline soils. In this study, 12 rice accessions were screened for tolerance to salt at seedling stage using morphological and molecular methods. The study was carried out in a hydroponic system using Hoagland solution. Scoring was done using the modified standard evaluation score (SES) system after 14 days of...

Author(s): Uyoh E. A., Ntui V. O., Umego C., Ita E. E., and Opara C.  

Effective method to control Vibrio mimicus infection in channel catfish Ictalurus punctatus

July 2019

Recently, a skin ulcerative disease caused by Vibrio mimicus has led to heavy economic losses in catfish, including yellow catfish, southern catfish, and Zhengchuan catfish in China. Currently, there was no effective method of controlling the outbreak of this disease. In this study, the bacterial isolates were obtained from dying channel catfish and identified as V. mimicus, which consist of formalin-inactivated V....

Author(s): Yan-Wei Li, Xiang Zhang , Yi-Jie Cai, Shu-Yin Chen, Hong-Yan Sun# and Xue-Ming Dan#

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