African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12218

AJB Articles

February 2019

Comparison of oil quality extracted from selected conventional and non conventional sources of vegetable oil from Malawi

In this study, oil quality with respect to physicochemical and phytochemical characteristics extracted from nonconventional seed oil namely Moringa oleifera, Adansonia digitata, Parinari curatellifolia and Cajanus cajan and conventional seed oil namely soybean (Glycine max) and groundnut (Arachis hypogaea) was assessed and compared. Results showed that there were significant differences in various quality parameters...

Author(s): Lesten Eliez Chisomo Chatepa, Hankie Uluko and Kingsley Masamba  

February 2019

Regulation of a dominant glutathione S-transferase and Na+/K+ by spermidine under salinity in onion

Spermidine (Spd) is one of the most important polyamines (PAs) showing important roles in growth, development and stress responses in plants. The role of Spd in regulation of a dominant glutathione S-transferase (GST, E.C.2.5.1.18) and ionic balance of Na+ and K+ in onion leaves were examined. Onion GSTs were separated from fresh bulb tissue using DEAE-cellulose chromatography. Three GSTs were eluted at 56, 120 and 169...

Author(s): Tanjina Islam, Md. Rezwan Molla, Reshma Sultana, Md. Bazlur Rahman, Munshi Rashid Ahmad and Md. Motiar Rohman  

February 2019

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Eighteen ISSR primers (between Simple Repeated Sequences) were used to estimate the genetic diversity of Angolan cassava populations in order to identify genetic variabilities and their potential to be used in the diversification of cassava crops in Angola. In order to do so, three populations from three Angolan provinces were analyzed (Cuanza Norte, Uige and Malanje), which totaled 40 genotypes. In total, 116 bands...

Author(s): Sandra Domingos João Afonso, Ricardo Franco Cunha Moreira, Carlos Alberto da Silva Ledo, Cláudia Fortes Ferreira, Vanderlei da Silva Santos and Pascoal António Muondo  

February 2019

Antioxidant, cytotoxic, and antidiabetic activities of Dendropanax morbifera extract for production of health-oriented food materials

Antioxidant, cytotoxic and anti-diabetic effects of fermented and non-fermented Dendropanax morbifera extracts were compared to assess the potential utility of this species in the development of health-oriented food. The non-fermented extract (NFDE) was obtained from leaves and branches of D. morbifera and the fermented extract (FDE) was prepared by inoculation with Lactobacillus plantarum and Lactobacillus brevis after...

Author(s): KiBeom Lee and Andy Kim  

February 2019

Enhancement of saponin accumulation in adventitious root culture of Javanese ginseng (Talinum paniculatum Gaertn.) through methyl jasmonate and salicylic acid elicitation

Javanese ginseng (Talinum paniculatum) is an herb and one of the traditional medicinal plants in Indonesia which accumulate saponin in the root organ. However, slow root growth hampers the accumulation of the compound in this plant. Therefore, in vitro culture of adventitious roots offers an alternative way to increase the production of saponin. Furthermore, this study was performed to investigate the effect of methyl...

Author(s): Ahmad Faizal and Anggita Veningtia Sari  

January 2019

Obtainment, applications and future perspectives of palm kernel

Of African origin and with an estimated world production of 6.85 million tons, palm kernel oil contributes about 3% of the total world production of oils and fats. With such production, the world waste generation was about 10,026 × 103 tons in 2017, which represents an important environment issue. The present work aimed to review the literature on the main methods of extraction, applications, and future...

Author(s): Wanessa Almeida da Costa, Cinthya Elen Pereira de Lima, Fernanda Wariss Figueiredo Bezerra, Mozaniel Santana de Oliveira, Priscila do Nascimento Bezerra, Flávia Cristina Seabra Pires, Ana Paula de Souza e Silva, Jorddy Neves da Cruz, Sebastião Gomes Silva, Pedro Alam de Araújo Sarges and Raul Nunes de Carvalho Junior,  

January 2019

Antimicrobial activity of Basidiomycetes fungi isolated from a Kenyan tropical forest

Microbial diseases remain to be a major global public health challenge. Their devastating effects have been exacerbated by the development of drug resistant strains. On the other hand, the biodiversity of tropical Basidiomycetes fungi is well recognized as an untapped source of potential bioactive compounds for the development of novel antimicrobials. Thus, in the current study, Basidiomycetes’ fruiting bodies...

Author(s): Winnie Chemutai Sum, Stephen Abwao Indieka and Josphat Clement Matasyoh

January 2019

Effect of cutting position and indole butyric acid (auxin) concentration on rooting response of Araucaria heterophylla

The effects of cutting position (tip, middle and basal) and concentration of indole butyric acid (IBA) (0, 5, 7.5 and 11 g/L) on root and shoot growth of Araucaria heterophylla were evaluated to develop a method for vegetative propagation for this tree species. Leaf number, number of adventitious roots, root length and survival rates were measured. These parameters were significantly influenced by the interactive effect...

Author(s): Abera Tilahun, Begashaw Manahlie, Getachew Abebe and Genet Negash  

January 2019

In vitro regeneration of two grapevine (Vitis vinifera L.) varieties from leaf explants

The traditional way of grapevine (Vitis vinifera L.) propagation is time consuming and allows disease transmission from generation to generation. Moreover, it is difficult to improve this crop through conventional plant breeding methods. Therefore, the objective of this study was to develop efficient in vitro regeneration protocol for ‘Canonannon’ and ‘Chenin Blanc’ varieties of grapevine using...

Author(s): Fikadu Kumsa and Tileye Feyissa  

January 2019

Plant biotechnology: A key tool to improve crop production in Rwanda

Rwanda’s economy relies on agriculture; nevertheless, crop production remains insufficient for both local consumption and exportation. Overall, enough agriculture yields in Rwanda to ensure food security has not yet been achieved regardless of more than 87% population engaged in agriculture activities. In this context, this study aimed at gathering the information on Rwanda’s agriculture based on different...

Author(s): Leonce Dusengemungu, Clement Igiraneza and Sonia Uwimbabazi  

January 2019

Is the introgression of Lobi/Baoulé cattle by zebuine genes in Burkina Faso Lobi cattle threatened?

Burkina Faso livestock is made up of two main cattle population, namely Zebuine and Taurine. Transhumance and settlement of Zebu cattle breeders in tsetse challenged areas lead to cross-breeding Zebu and Taurine. Introgression of the Zebu cattle may have changed the structure of the trypanotolerant Lobi/Baoulé breed. The objective of the present study was to appreciate the introgression of Zebu genes into...

Author(s): Albert Soudré, Moustapha Gréma, Stéphane A. R. Tapsoba, Amadou Traoré, Salifou Ouédraogo-Koné, Maria Wurzinger, Simone Müller, Olivier Hanotte, Anicet Georges Ouédraogo and Johann Sölkner  

January 2019

Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

Yellow rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat in Ethiopia. Poor knowledge of resistance genes deployed in Ethiopian wheat cultivars is one major factor for recurrent epidemics of rust diseases. Molecular marker based gene identification showed the presence of Yr1, Yr4, Yr6, Yr7, Yr9, Yr10, Yr17 and Yr18 in various frequencies, whereas Yr8 was not detected in...

Author(s): Woubit Dawit, Ayele Badebo, Bekele Hunde, Daniel Kassa and M. S. Röder  

January 2019

Evaluation of the morphological and quality characteristics of new papaya hybrid lines in Kenya

Papaya (Carica papaya L.) is among the most grown fruit crops worldwide with high economic and nutritional value. In Kenya, the papaya industry relies heavily on imported varieties and farmers’ selected seed whose quality is not known. Therefore, the morphological and quality characteristics of mature fruits of eight newly developed papaya hybrids and their control, Sunrise solo were assessed using papaya...

Author(s): Gaudence Nishimwe, Janet Chepng’etich Kosgei, Everlyn Musenya Okoth, George Ochieng’ Asudi, and Fredah Karambu Rimberia  

January 2019

Decolourization of synthetic dyes by laccase enzyme produced by Kluyveromyces dobzhanskii DW1 and Pichia manshurica DW2

Industrialization has come with environmental challenges. Industries like paper, printing, textile, leather and so on widely use chemical dyes whose waste treatment or degradability is difficult. Among various methods employed, the use of microbial enzymes is the most effective. The study aimed at producing laccase from identified yeast strains for potential industrial use in dye decolourization. Laccase produced by...

Author(s): Sherifah Monilola Wakil, Seun Andrew Eyiolawi, Kehinde Olamide Salawu and Abiodun Anthony Onilude  

January 2019

Enhancement of anaerobic batch digestion of spineless cacti (Opuntia ficus indica) feedstock by aerobic pre-treatment

One of the best options for African countries to meet rural energy needs is to grow care-free crassulacean acid metabolism plants on a massive scale in waste lands. This can enable bioenergy production without disrupting food supplies and hence sustainable energy supply for the future. Opuntia ficus indica is an ideal plant for arid regimes but has barely been studied as a potential bioenergy source. This study...

Author(s): Hawa Myovela, Anthony Manoni Mshandete and Samuel Imathiu  

January 2019

Evaluation of the diversity in qualitative traits of Bambara groundnut germplasm (Vigna subterranea (L.) Verdc.) of Côte d’Ivoire

The objectives of this study were to assess phenotypic diversity of Bambara groundnut germplasm from Côte d’Ivoire using qualitative traits and to understand the genetic diversity at different levels. Hundred and one accessions collected from four agro-ecological zones (central, eastern, northern, western) were characterized in a randomised complete block design with three replications. Thirteen qualitative...

Author(s): Beket Séverin BONNY, Dagou SEKA, Koffi ADJOUMANI, Kouamé Guillaume KOFFI, Léonie Clémence KOUONON and Raoul Sylvère SIE  

December 2018

Degradation kinetics of 2,4-dichlorophenoxyacetic and atrazine by Trametes versicolor (L.:Fr.) Pilát

The degradation kinetics of different sources of 2,4-dichlorophenoxyacetic (2,4-D) and atrazine by the natural strain Mo008 of the basidiomycete fungus Trametes versicolor (L.:Fr.) Pilát was studied, knowing that in this process the strain used produces an enzyme complex composed of manganese peroxidase (MnP), lignin peroxidase (LiP) and laccase, and the response on the global consumption rate and yield is...

Author(s): Ernesto Hernández Mendieta, Dagoberto Guillén Sánchez, Iran Alia Tejacal, Victor López Martínez, María Andrade Rodríguez, Edgar Martínez Fernández, Manuel Huerta Lara, and Catalina Rubio Granados  

December 2018

Isolation and characterization of Ralstonia solanacearum strains causing bacterial wilt of potato in Nakuru County of Kenya

In Africa, cultivation of potato (Solanum tuberosum) represents an important food source and income generation. However, its productivity is constrained by biotic and abiotic stresses. Bacterial wilt caused by Ralstonia solanacearum is an important constraint to the world potato industry. In Kenya, the disease affects 77% of potato farms causing yield losses of up to 100%. Control methods are limited mainly due to the...

Author(s): Rostand Romeo Chamedjeu, Joel Masanga, Viviene Matiru, and Steven Runo  

December 2018

Dynamics of the concentration of the hormones triiodothyronine and thyroxine at the beginning of the reproductive season and the effect of application of iodine in Nelore heifers bred in pasture

The aim of this study was to evaluate the dynamics of triiodothyronine (T3) and thyroxine (T4) hormones, their relation with body condition score (BCS) and reproductive stage of 248 Nelore heifers, and the effect of iodine application on the induction of cyclicity of 217 anoestrus heifers in pasture at the beginning of the reproductive season. At day zero (D0), an assessment of the BCS was performed. An examination of...

Author(s): Adriana Almeida Fonseca Ruy, Karen Martins Leão, Mariana da Mata Silveira, Viler Carrijo Oliveira, Francisco Ribeiro de Araújo Neto and Marco Antônio Pereira da Silva

December 2018

Comparison of antimicrobial properties of two spices commonly consumed in Nigeria and effect of temperature on their antioxidant properties

The study compared the antimicrobial activities of essential oils (EOs) from Piper guineense (Uziza) and Xylopica aethiopica (Uda) using diffusion and dilution assays, and evaluated their antioxidant properties at different temperatures. Comparison of the antimicrobial activities was determined using well-in-agar diffusion, disc diffusion and broth dilution methods against Escherichia coli, Salmonella sp and Bacillus...

Author(s): Ogueke, Chika C., Azeke, Ehijie Augusta, Owuamanam, Clifford I., Ojukwu, Moses, Agunwah, I. M., Barber, L. I. and Bede, E. N.  

December 2018

Effects of heat sources on the levels of polycyclic aromatic hydrocarbon in selected fish samples from Ogidingbe (Escravos Estuaries)

The riverine areas of the Niger-Delta region of Nigeria are popularly known for their smoked fish from both fresh and salt water. To the people living in these areas, smoking is a method of preservation as the fish is known to undergo spoilage within 8 h of harvest from its natural habitat; though not without the potential health hazard associated with smoked foods. During smoking, polycyclic aromatic hydrocarbons...

Author(s): Erhabor Destiny, Edjere Oghenekohwiroro and Ejeomo Christopher

December 2018

Clausenidin upregulated p53 and caused apoptosis in HT-29 tumor cell lines

Clausenidin is a pyranocoumarin majorly found in medicinal herbs of the Rutaceae family used to treat cancer patients locally in Asia. The compound is presumed to have anti-cancer cell effect but its exact mechanism of action is still unknown. The study aimed to evaluate the effect of pure clausenidin on p53-mediated apoptosis as well as other cell death pathways in colon cancer (HT-29) cell lines. The...

Author(s): Peter M. Waziri, Rasedee Abdullah, Rosita Rosli, Abdul Rahman Omar, Ahmad Bustamam Abdul, Nur Kartinee Kassim, Ibrahim Malami, Imaobong C. Etti, Palanisamy Arulselvan and Ashwaq Shakir Al-Abboodi  

December 2018

Quali-quantitative characterization of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira): macroscopic, microscopic, physico-chemical and microbiological parameters

The objective of the present study was to evaluate the external and organoleptic characters, microscopic, physico-chemical, chromatic and microbiological parameters of the honey from Myracrodruon urundeuva allemão (Anacardiceae - Aroeira) of the Jequitinhonha River Valley / Brazil, in order to create its geographical indication. In the first stage a data survey was carried out to characterize the production of...

Author(s): Eliane Macedo Sobrinho Santos, Hércules Otacílio Santos, Juliana Rezende Sá Miranda Gonçalves, Anna Christina Almeida, Igor Viana Brandi, Alexsander Rodrigues Cangussu, Janainne Nunes Alves, Ricardo Jardim Neiva, Grazielle Layanne Mendes Santos, Maria Izabel de Jesus Viana, Bruna Ruas Santos Araújo, Thalita Cordeiro Santos and Kattyanne Souza Costa

December 2018

Determination of trace elements and heavy metals content of green and black tea varieties consumed in Iran

The aim of the present study was to determine the level of selected heavy metals in green and black tea varieties consumed in Iran. Samples were prepared with the acid digestion method and measured by polarography. The mean concentration of Zn, Cd, Pb and Cu was 0.505±1.632, 0.417±0.268, 0.325±0.249 and 4.151±3.739 mg/100 g for black tea, and 2.442±4.003, 0.284±0.198,...

Author(s): Behrooz Jannat, Naficeh Sadeghi,, Mohammad Reza Oveisi, Masoomeh Behzad, Manan Hjimahmoodi and Morteza Ebrahimi  

December 2018

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated...

Author(s): Nguyen Thi Thu#, Vu Hong Quang#, Mai Van Tan#, Vu Đuc Lam, Nakano Toshitsugu, Nguyen Thi Hue, Nong Thi Hue, Nguyen Viet Long, Nguyen Van Hoan, Vu Van Liet, Nguyen Thi Mien, Tran Thi Lien, Nguyen Dinh Trung and Tran Thi Thu Hoai

December 2018

Superoxide dismutase activity and jasmonic acid during in vitro-ex vitro transition of pineapple (Ananas comosus (L.) Merr.) micropropagated plantlets

Recent agriculture is characterized by intensive and cleaning productions, which need seeds with high quality in large quantities bonded by in vitro culture labs. Nevertheless, in vitro ex vitro transition and during acclimatization losses occur due to the death of plantlets unable to survive this abiotic stress. Reactive oxygen species production during jasmonic acid-induced changes of previous transition was...

Author(s): González-Olmedo J. L., Garza-García Y., Mbogholi A., Rodríguez-Escriba R. C., Aragón C. E., Rodríguez R., and Moreno A.  

December 2018

Preliminary study on rice straw degradation using microbial inoculant under shake flask condition

Abundance and unmanaged agriculture residues lead to unutilized resource waste and environmental pollution. Application of microbial technology to manage agriculture waste could produce value-added product. A preliminary study on biodegradation of rice straw using different potential microorganisms was tested under controlled environment. Three different inoculant cultures were used to observe their efficiency in rice...

Author(s): Nurul Ain Abu Bakar, Mohammad Hariz Abdul Rahman, Nur Alyani Shakri, Syuhaidah Abu Bakar and Abhar Abdul Hamid

December 2018

DNA extraction from silica gel-preserved common bean (Phaseolus vulgaris L.) leaves

Extraction of non-degraded and contaminant-free DNA from field specimen requires collection under liquid nitrogen which is not readily available in resource constrained laboratories in low and middle income countries (LMICs). A method of extracting DNA from silica gel-preserved common bean (Proteus vulgaris L.) leaves is presented. The method, which does not involve the use of phenol, chloroform or isoamyl alcohol also...

Author(s): Male Allan Ssekamatte, Kato Fred and Mukankusi Clare Mugisha

November 2018

Marker-assisted backcrossing for associating nutritional quality to drought tolerance in barley

Backcross breeding enhanced by marker assisted selection is currently the most powerful method used to transfer one or a few genes controlling specific traits from one line into another. This method was used to cross naked barley variety, “Tombari” known as A, having high nutritional quality with hulled variety, “Giza131” called B, highly tolerant to drought. The objective of this work is to...

Author(s): Amani Ben Naceur, Sameh Mnasri, Hatem Cheikh-M’hamed, Chedly Abdelly and M’barek Ben Naceur  

November 2018

Identification and mapping of quantitative trait loci associated with soybean rust (Phakopsora pachyrhizi) resistance in genotype UG 5

Soybean rust, Phakopsora pachyrhizi, is one of the most serious and widespread foliar diseases of soybean causing high yield losses world-wide. The objective of this study was to identify and map quantitative trait loci (QTLs) resistant to soybean rust in genotype UG 5. Ninety-seven F2 mapping plants, obtained from a cross between Wondersoya and UG 5, were used for this study. Quantitative trait locus analysis using QTL...

Author(s): Hailay Mehari Gebremedhn, Ulemu Mercy Msiska, Miesho Belay Weldekidan, Fentaw Abate Asmamaw, Akech Winnifred, Dramadri Isaac Onziga, Thomas Lapaka Odong, Patrick Rubaihayo and Phinehas Tukamuhabwa

November 2018

Characterization of fifteen microsatellite loci and genetic diversity analysis for the Ghanaian food security crop Solenostemon rotundifolius (Frafra potato)

Frafra potato (Solenostemon rotundifolius) is an important food crop indigenous to Western Africa and a member of the Labiaceae (mint) family. It is nutrient-rich and drought tolerant; however, despite its potential as a food security crop, it has not received the research support needed to promote its widespread adoption and safeguard its diversity. As a result, Frafra potato is becoming extinct and restricted to...

Author(s): Lichun Hua, Đenita Hadziabdic, Naalamle Amissah, Marcin Nowicki, Sarah L. Boggess, Margaret Staton, Nianjun Teng and Robert N. Trigiano

November 2018

Evaluation of genetic diversity of pearl millet (Pennisetum glaucum L.) landraces from West Africa using microsatellite markers

Pearl millet (Pennisetum glaucum L.) is an important staple food crop, especially in arid and semi-arid regions of West Africa. However, the genetic diversity of pearl millet in these regions is poorly understood, and yet it is useful in identification of landmarks for in situ germplasm conservation and breeding programs, particularly in establishment of heterotic groups. Therefore, the aim of this study was to assess...

Author(s): Mary N. Onsarigo and Dionysious K. Kiambi  

November 2018

Comparison of four DNA extraction methods in Heliconia (Heliconia L.)

Molecular analysis in plants requires the use of high quality DNA. Currently, there are a myriad of DNA extraction protocols, which still seems that their applicability across different species is not straightforward. The aim of the present study was to evaluate four different cetyl trimethylammonium bromide (CTAB)-based DNA extraction protocols for Heliconia species from lyophilized fully mature leaves. A split-plot...

Author(s): Olvera S., Cortés-Cruz M., Contreras-Toledo A., Ortiz S., Avendaño-Arrazate C., Olivera A., Canul J. and Grajales M.  

October 2018

Bioremediation potentials of sunflower and Pseudomonas species in soil contaminated with lead and zinc

This study was conducted to assess the ability of sunflower (Tithonia diversifolia) inoculated with Pseudomonas species to remediate soils contaminated with heavy metals (lead and zinc). The contaminants were added to 20 kg soil in pots as lead nitrate (Pb (NO3)2) and zinc nitrate (Zn (NO3)2); 400 mg/l metal concentrations were added to 20 kg soil in pots. The experimental design was a randomized complete block design...

Author(s): Elizabeth Temitope Alori, Abiodun Joseph, Ojo Timothy Vincent Adebiyi, Ajibola Patrick Aluko and Chidera Onyekankeya  

October 2018

Applications of genomic copy number variations on livestock: A review

Genomic copy number variations (CNVs) have significantly modified genomic regions during inversions, translocations, duplications and deletions that keep shaping phenotypic variation through changes in gene dosage, regulation and transcript structure. The objective of this review was to discuss the current application of CNVs on domestication, adaptations, productions, coat colour, morphology and diseases of livestock,...

Author(s): Habtamu Abera Goshu, Min Chu and Ping Yan

October 2018

Phenotypic and genotypic characteristics of cowpea rhizobia from soils of Ethiopia

Phenotypic and genotypic characteristics of cowpea rhizobia indigenous to soils of Ethiopia are unknown. Forty indigenous cowpea rhizobial isolates were collected from cowpea-growing areas of the country and were characterized for their growth and genetic properties. Based on their cultural characteristics, the isolates were categorized into fast (FG), slow (SG), and extraslow-growings (ESG). The FG, SG, and ESG...

Author(s): Girmaye Kenasa, Fassil Assefa and Mussie Y. Habteselassie  

October 2018

Propolis as natural additive: A systematic review

Propolis extract is a resin collected by the bees and contains numerous active substances with a recognized pharmaceutical potential use. The aim of this study was to conduct a systematic review of studies with the use of propolis as a food additive and the microencapsulation technology for incorporation of the product in food. Studies in Portuguese, English and Spanish were identified from online databases, Lilacs,...

Author(s): Marly Silveira Santos, Maria Leticia Miranda Fernandes Estevinho, Carlos Alfredo Lopes de Carvalho, Karina Teixeira Magalhães-Guedes, Rosane Freitas Schwan and Rogeria Comastri de Castro Almeida

October 2018

Anti-diabetic and some haematological effects of aqueous and ethanol leaf extract of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats

This study investigates the anti-diabetic and some hematological effects of aqueous and ethanol leaf extracts of Eriosema psoraleoides in alloxan-induced diabetic Wistar rats. Forty-eight albino mice were used for the lethal dose (LD50) study, while twenty-eight Wistar rats were used for the diabetic and haematological study. The result of the phytochemical analysis shows some important phytochemicals that could confer...

Author(s): Nduka, Florence O., Ogugua, Victor N., Joshua, Packer E., Okpachi, Victor E., Gometi, Sandra A. and Nwigwe, Juliet O.  

October 2018

Comparative survey of three processes used for the extraction of total phenol content and total flavonoid content of Anacardium occidentale L. and the assessment of its antioxidant activity

Three processes were used for the extraction of total phenol and total flavonoid contents of Anacardium occidentale L. and to determine its antioxidant activity. Whatever the extraction process was, the total flavonoid and total phenol contents were of the same order. With the microwave-assisted extraction (MAE), 666.07 mg gallic acid equivalent (GAE)/100 g dry matter (DM) of total phenol content was obtained from the...

Author(s): MADJITOLOUM BETOLOUM Salomon, TALLA Emmanuel, NYEMB Jean Noël, NGASSOUM Martin Benoît, TSATSOP TSAGUE Roli Karole and MAHMOUT Yaya  

October 2018

Resveratrol inhibits cell viability and proliferation via apoptotic pathways in ovarian cancer

Ovarian cancer is the most common female malignancy and the main cause of death from gynecological malignancies. Resveratrol (3,4,5 tri-hydroxystilbene) is a phytoalexin and a polyphenolic compound present in human dietary material such as peanuts, mulberries, grapes and red wine. We demonstrated that resveratrol depressed the proliferation of HO8910PM cells in a dose-dependent and time-dependent manner in vitro (P <...

Author(s): YAO Jing-ping, XU Fei, LI Rong, LI Meng and QU Jun-wei  

September 2018

Next-generation sequencing platforms for latest livestock reference genome assemblies

Next-generation sequencing (NGS) or high-throughput sequencing platforms refers to different modern sequencing technologies that allow scientist to sequence millions to billions of reads (base pairs) in a single, much more quicker and cheaper cost than the previously used Sanger (first generation) sequencing method. Next-generation sequencing platforms classified as second generation sequencing platforms which require...

Author(s): H. Gatew and G. M. Tarekegn,  

September 2018

The effect of different nutrient media on in vitro shoot and root proliferation of Vanilla planifolia Jacks. ex Andrews

Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW) nutrient media are among the most widely used for micropropagation of orchid species. Therefore, this study was conducted to evaluate the effect of these growth media on in vitro shoot and root proliferation of Vanilla planifolia by using nodal segment as explants. Three different media; Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW) were...

Author(s): Noorhazira Sidek, Nuraini Shafinaz Mohd Anuar, Laila Naher and Kharul Azmi Mu’azzam Abdul Rahman

September 2018

Genetic diversity of blackberry (Rubus subgenus Rubus Watson) in selected counties in Kenya using simple sequence repeats (SSRs) markers

Genetic diversity of blackberry (Rubus subgenus Rubus Watson) is essential for efficient breeding and improvement of its pomological traits and yield. In this research, simple sequence repeats (SSRs) were used to determine the genetic diversity of 90 blackberry accessions collected from six different counties in Kenya. From 11 SSR markers used to genotype the blackberry accessions, a total of 127 alleles were generated....

Author(s): Jared A. Ochieng, Maurice E. Oyoo, Robert M. Gesimba, Paul C. Korir, Pascal P. Okwiri Ojwang’ and James O. Owuoche

September 2018

Characterization of fluorescent Pseudomonas from Oryza sativa L. rhizosphere with antagonistic activity against Pyricularia oryzae (SACC.)

The aim of this work was to select and characterize native Cuban fluorescent Pseudomonas from the rhizosphere of Oryza sativa with in vitro and in vivo antagonistic activity against Pyricularia oryzae. A total of 200 isolates were recovered with the typical growth and cultural characteristics of fluorescent Pseudomonas. The results showed that 12.5% of total isolates were capable of inhibiting mycelial growth, with...

Author(s): Annia Hernández-Rodríguez, Yanelis Acebo-Guerrero, Gema Pijeira Fernández, Acela Díaz de la Osa and Gloria Maria Restrepo-Franco  

September 2018

Chromium (III) and its effects on soil microbial activities and phytoremediation potentials of Arachis hypogea and Vigna unguiculata

The aim of this research was to evaluate the impact of chromium (III) nitrate on soil microbial activities and growth performance and phytoremediation potentials of two staple leguminous crops, namely cowpea (Vigna unguiculata) and groundnut (Arachis hypogea). Pristine sandy loam soil samples were polluted with nitrate salts of chromium (III) at four different levels (50, 100, 200 and 400 mg/kg) in triplicates. There...

Author(s): Chibuzor Nwadibe Eze, Chuks Kenneth Odoh, Emmanuel Aniebonam Eze, Paul Ikechukwu Orjiakor, Simeon Chukwuemeka Enemuor and Uchenna Jude Okobo  

September 2018

Phytochemical and proximate composition of cucumber (Cucumis sativus) fruit from Nsukka, Nigeria

Cucumber (Cucumis sativus L.) is very common, cultivated throughout the world and often eaten as a raw vegetable without cooking. In this study, the phytochemical and proximate compositions of cucumber were investigated. Quantitative phytochemical analysis of the homogenate of C. sativus fruit showed that reducing sugars (574.36 ± 3.88 mg/g) was highest amount when compared to other phytochemicals, alkaloids...

Author(s): Uzuazokaro Mark-Maria Agatemor, Okwesili Fred Chiletugo Nwodo and Chioma Assumpta Anosike  

September 2018

Trichoderma atroviride 102C1: A promising mutant strain for the production of a β-glucosidase, -xylosidase and -L-arabinofuranosidase activities using agroindustrial by-products

Holocellulolytic accessory enzymes are very important in assisting hydrolysis of biomass. The use of these enzymes in the enzymatic hydrolysis of plant biomass is very important for obtaining building blocks in the concept of biorefinery. In previous studies, the mutant strain Trichoderma atroviride 102C1 was tested for production of endoglucanases, FPases and endoxylanases. This study aimed at evaluating the efficiency...

Author(s): Jéssica Caroline Araujo SILVA, André Luiz Grigorevski-Lima, Elba Pinto da Silva BON, Rosalie Reed Rodrigues COELHO and Rodrigo Pires do NASCIMENTO

September 2018

Genetic diversity of seed storage protein in the Ethiopian garden cress (Lepidium sativum L.)

The Ethiopian garden cress (Lepidium sativum L.) is an important crop extensively used as food and medicine. In this study, total seed storage proteins of 112 garden cress genotypes collected from diverse growing regions in Ethiopia were investigated to assess patterns of genetic diversity and relationships. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), a total of 1774 stable protein bands...

Author(s): Legesse Tadesse, Firew Mekbib, Adugna Wakjira and Zerihun Tadele

September 2018

Identification and evaluation of secondary metabolites by gas chromatography-mass spectrometry (GC-MS) in native strains of Trichoderma species

Trichoderma species is a heterotrophic saprobion organism, which competes with other microorganisms, preventing them from developing. This fungus has mechanisms of action, among which are: production of antibiotics and metabolites, and hyperparasitism, which also promotes the induction of systemic resistance in plants. In this study, five native Trichoderma strains which were collected from agricultural land in...

Author(s): Martínez-Padrón Hadassa Yuef, Torres-Castillo Jorge Ariel, Rodríguez-Herrera Raúl, López-Santillán José Alberto, Estrada-Drouaillet Benigno and Osorio-Hernández Eduardo

September 2018

Heterozygote polymorphisms of ARG16GLY and GLN27GLU ADRB2 gene is risk protective for obesity in Javanese population of Indonesia

The relationship between obesity and insulin resistance is influenced by various factors, including genetics. ADRB2 gene polymorphisms of Arg16Gly and Gln27Glu showed inconsistent results toward obesity and insulin resistance in some populations. This study aimed to investigate the frequency of the genetic polymorphisms of Arg16Gly and Gln27Glu of the ADRB2 gene and to analyze risk factors for obesity and insulin...

Author(s): Nazula Rahma Shafriani, Pramudji Hastuti and Ahmad Hamim Sadewa  

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