African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12485

AJB Articles

Molecular characterization of Escherichia co-resistance genes from chicken meat

April 2022

Escherichia coli multi-resistance to a variety of antimicrobials is a result of gene mutation on plasmids, integrons and transposons. The aims of this work were to: (1) detect genotype and phenotype antibiotic resistance genes in E. coli, and (2) determine whole-genome sequencing to discover E. coli gene multi-drug resistance in chicken meat. Samples were gathered, processed, and analysed bacteriologically; thereafter...

Author(s): Nagwa Thabet Elsharawy, Hind A. A. Al-Zahrani and Amr A. El-Waseif

Agronomic performances of temporary immersion bioreactor-derived potato microtubers in a Peruvian low input cropping agriculture system

March 2022

In Peru, potato cultivation represents 25% of the agricultural gross domestic product yet only 0.2% of the agamic seed used is from certified sources. The use of temporary immersion bioreactors (TIB) has improved the quality of microtubers micropropagated along with savings in costs of production. The current study investigated the agronomic performances of Peruvian Canchan potato microtubers derived from TIB (basic...

Author(s): María de Lourdes Tapia y Figueroa, José Faustino Beraún Tapia, Elliosha Hajari, Maritza Escalona, Hervé Etienne, and José Carlos Lorenzo

Leaf microscopy, high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses of Croton zambesicus Müll.-Arg. leaf (Family: Euphorbiaceae) in Nigeria

March 2022

This study was done to get information on the chemical composition and the internal structure of the plant which could aid in its further characterization. The study is aimed at determining the epidermal leaf anatomy, HPLC of the hot water extract, chemical constituents of the oil from the leaves by GC-MS and chemo-microscopic properties of Croton zambesicus for proper identification of the plant. The leaf epidermal...

Author(s): Ugbabe G. E. and Dalyop J. D.

Liver enzyme evaluation and NAT2 polymorphism in patients on anti-tuberculosis and antiretroviral drugs at Jamot Hospital in Yaoundé-Cameroon

March 2022

Hepatotoxicity is reported frequently as an adverse reaction during tuberculosis (TB) and HIV treatment. This study aimed to investigate the incidence of antiretroviral and anti-tuberculosis drug-induced liver enzymes activities variation in TB and TB-HIV co-infected patients at Jamot Hospital in Yaoundé-Cameroon. From April 2018 to May 2019, 336 treatment-naïve TB patients were enrolled. Liver enzymes (AST,...

Author(s): Ambassa Axel Cyriaque, Tiani Lionel Ulrich, Djuidje Ngounoue Marceline, Assam Assam Jean Paul, Andoseh Généviève, Kamdem Thiomo Diane, Tchinda Fossi Cédric, Nkah Numfor Leonard, Gonsu Kamga Hortense, kouanfack Charles, Pefura Yone Eric Walter, Ntoumi Francine, and Penlap Beng Véronique,

Assessment of proximal, mineral composition and content of vitamin A and C of leaves and flowers from Lippia multiflora vegetable in Benin

March 2022

Lippia multiflora is a plant whose leaves and flowers are used as a food in Benin, so it is important to determine the nutritional properties of leaves and flowers of L. multiflora. Biochemical analyses for the determination of macronutrients, mineral salts and vitamins A and C on leaves and flowers of this leaf vegetable were done. The result indicates that the contents in leaves and flowers are, respectively:...

Author(s): Hihoto Wilfrid Djengue, Arlette Adjatin, Rock Djehoue, Zaki Bonou-gbo, Codjo Tiburce Odjo, Zinsou Com Koukpo, Arsène Jacques Fassinou, Gérard Tiko, Hyacinthe Ahissou and Alexandre Dansi

A study of the antimicrobial activity of Psidium guajava L. and Lawsonia inermis leaf extracts against some foodborne pathogens

March 2022

The study aims to determine the antibacterial activity of Psidium guajava and Lawsonia inermis leaf extracts against some foodborne pathogens. The leaf samples were collected, rinsed, dried, ground, and then stored. The leaf powder was soaked in ethanol and boiled in distilled water. Then, it was filtered. The antibacterial activity of P. guajava and L. inermis leaf extracts against Salmonella enteritidis,...

Author(s): Nagwa T. Elsharawy, and Afra Mohammed Baghdadi

Inclusion of African winged termites (Macrotermes nigeriensis) improves the nutrients and quality of fermented cassava mahewu

February 2022

Cassava mahewu is a non-alcoholic fermented beverage used by school children, but it has low contents of protein and micronutrients. This study examined the effect of inclusion of African winged-termite (AWT) on the nutritional quality and acceptability of cassava mahewu. Cassava flour was replaced with AWT at varying proportions of 100:0 (as control), 90:10, 80:20, 70:30 and 60:40% and fermented to obtain cassava...

Author(s): Paul Ndubuisi ANYIAM, Chinedu P. NWUKE, Goodluck Chibuikem ADIMUKO, Chinaza Precious NWAMADI, Elsa Maria SALVADOR, Grace Folake AJIBADE and Elizabeth Chinomso MAXWELL

Polymorphism of the heat shock protein 70 gene in indigenous chickens from different agro-climatic zones in Kenya

February 2022

Climate change leads to heat stress, which is one of the most challenging environmental conditions affecting poultry. Studying thermotolerance in poultry is crucial because it may identify genetic lines that can withstand heat stress. This study aimed to investigate polymorphisms in the heat shock protein 70 (HSP70) gene in indigenous chicken. In Kenya, we collected 277 blood samples from selected chicken ecotypes from...

Author(s): Grace Moraa Kennedy, Stephen Njuguna Kuria, Philip Murunga Panyako, Jacqueline Kasiiti Lichoti and Sheila Cecily Ommeh

Biogenic synthesis of silver nanoparticles using Azadirachta indica methanolic bark extract and their anti-proliferative activities against DU-145 human prostate cancer cells

February 2022

Phytomedicine coupled with nanotechnology has shown possibilities in treating different ailments including cancer. In the current study, silver nanoparticles (AgNPs) were biogenically synthesized using methanolic extracts of Azadirachta indica (neem) barks and tested for cytotoxic and anti-proliferative activities on normal cells (Vero E6) and human prostate cancer cells (DU-145), respectively by resazurin assay....

Author(s): Shedrack Reuben Kitimu, Peter Kirira, Judith Sokei, Dominic Ochwangi, Peter Mwitari and Naomi Maina,

In vitro propagation of two commercially important bamboo species (Bambusa tulda Roxb. and Dendrocalamus stocksii Munro.)

February 2022

Bamboo plant is an important biodegradable raw material which can play important role in rejuvenation of Indian rural economy through positively impacting agricultural, industrial, energy and in environmental sectors. Traditional methods of vegetative propagation of this plant have insufficient multiplication rate. In the present investigation, in vitro bud break and aseptic culture establishment in relation to...

Author(s): Ajoy Kumar Choudhary, Priyanka Kumari and Sunita Kumari

Cryopreservation of plant genetic resources: A legacy for humanity

February 2022

Plant genetic resources are essential for agri-food security in the world and conservation of genetic diversity. Cryopreservation is an in vitro culture technique used for long-term plant conservation, by freezing the tissue at low temperatures usually with liquid nitrogen (-196ºC). During cryopreservation, cell division and metabolic activity of the explants are quiescent. There are different cryopreservation...

Author(s): Bibiana Tirado-Pérez and Gabriela Sandoval-Cancino

Diversity of Arbuscular mycorrhizal fungi in the three agroecological zones of the Central African Republic

January 2022

Arbuscular mycorrhizal fungi (AMF) are organisms that can establish symbioses with 80% of terrestrial plants. Soils were collected in three agroecological zones (AEZ) of the Central African Republic (CAR). The aim of this research is to study the diversity of AMF in the CAR. The spore extraction and enumeration, granulometric and physicochemical analyses of each soil were performed. The results showed that AEZ3 has a...

Author(s): Mathurin Djamndo DJASBE,, Hubert Dieu Béni ELIAN, Tahir Abdoulaye DIOP and Denis Omokolo NDOUMOU

Identification of potential seed storage protein responsible for bruchid resistance in common bean landraces from Tanzania and Malawi

January 2022

Bean bruchids are among the most devastating insect pests of common bean that can inflict huge losses in storage. To identify potential resistance to these pests, screening was performed at Sokoine University of Agriculture. Two resistant landraces were identified, viz Kalubungula and KK25. Recombinant inbred (RI) KSy, KSw and ML populations were created from crosses between Soya × Kalubungula, Soworo ×...

Author(s): Caroline Ng’homange Maro, Deogracious Protas Massawe, George Muhamba Tryphone, James Robert Myers, Joel William Davis and Paul Mbogo Kusolwa 

Rapid single-tube splice variants typing of the BF gene based on dual-primer RT-PCR amplification that influence resistance/susceptibility to Marek’s disease in chicken

January 2022

A new effective splice variants typing based on multiplex allele-specific dual-primer RT-PCR assay was developed in a single tube for the rapid detection of the exon 7 splice variant of the BF gene. With 2 pairs of primers, one pair was used for amplifying cDNA fragments containing exon 7 of the BF gene, the other does not contain exon 7 of the BF gene. The templates were amplified in one tube and the type of splice...

Author(s): Yuan-chang Jin, Yu-feng Li, Liang Huang, Jia-jun Zhou, Xue-fang Zhang,  Ran Ma, Meng-lin Lu, Mei-lin Hao, Gang Zeng  and Bo-ping Zeng

Isolation and characterization of multidrug resistance bacteria from hospital sewage samples, Maharashtra, India

January 2022

Multiple antibiotic resistance is a major cause of clinical infections worldwide. This study determined the pattern of multidrug bacterial resistance in hospital sewage samples from the Marathwada region of India. Forty-eight isolates of bacteria were obtained from 6 locations of Aurangabad.  An antibiotic sensitivity test was carried out using the disc diffusion method. Among all the antibiotics tested, the...

Author(s): Bhaurao Bapurao Choushette and Rajendra A. Satpute

Molecular and morphological identification of fungi causing canker and dieback diseases on Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl in lower Eastern Kenya

January 2022

Drought-tolerant multipurpose fruit trees Vangueria infausta (Burch) subsp. rotundata (Robyns) and Berchemia discolor (Klotzsch) Hemsl are native to Kenya. These fruit tree species are suitable for dryland agroforestry and support local communities with food, medicine, fodder and other necessities. Reports by the local communities indicate that the two species suffer from diebacks and cankers. The aim of this study was...

Author(s): Susan Karani, Jane Njuguna, Steven Runo, Alice Muchugi, Joseph Machua and Phoebe Mwaniki

Effect of foliar application of nitrogen-phosphorus-potassium fertilizers on nutrient uptake and protein content of maize

December 2021

The effect of foliar application of nitrogen-phosphorus-potassium (NPK) fertilizers on nutritional status and yield of maize (Zea mays L.) were investigated through a study conducted in a zonal agricultural research station of University of Agricultural Sciences, GKVK Bengaluru (India). The experiment was laid out in randomized complete block design. The treatments consisted of two levels of soil application (75 and...

Author(s): D. Nirere, S. R. Mbaraka, F. X. Rucamumihigo, F. Murorunkwere, F. R. Musana and H. Rukangantambara

Statistical modeling of effective doses in hormetic dose-response relationships by reparameterization of a bilogistic model for inverted U-shaped curves

November 2021

Hormesis is a biphasic dose (concentration)-response phenomenon characterized by low dose stimulatory and high dose inhibitory effects exerted by stressors on living organisms. Recently, there has been increased interest in the phenomenon and statistical models for its exploration in toxicological studies. The bilogistic model of Beckon et al. is one of such models for modeling biphasic dose-response relationships in...

Author(s): Christian O. Nweke, Oluchukwu R. Nwangwu, Chukwudi J. Ogbonna and Reuben N. Okechi

Genetic diversity analysis of sorghum [Sorghum bicolor (L.) Moench] races in Ethiopia using simple sequence repeats (SSR) markers

November 2021

Sorghum [Sorghum bicolor (L.) Moench], is believed to be originated in Ethiopia and Sudan. Although, many morphological and molecular diversity studies reveal the existence of genetic variations with sorghum populations, their distribution within basic races were not considered. Hence, the present study aimed to analyze the extent and distribution of genetic variation within basic Ethiopian sorghum landraces using SSR...

Author(s): Gamachu Olani, Tileye Feyissa and Tesfaye Disasa

Advances on the application of non-coding RNA in crop improvement

November 2021

Thanks to committed plant breeding researchers over the few decades, many problems associated with food supply and qualities have been improved. Food security is an exceptionally serious worldwide issue via world climate change, the increase in human population, and the use of plants for bioethanol production in current years. Improved tolerance to abiotic and biotic stress, resistance to herbicides, improved yield, and...

Author(s): Diriba Guta Dekeba

Antioxidant effects of ginger, garlic and onion aqueous extracts on 2-thiobarbituric acid reactive substances (2-TBARS) and total volatile basic nitrogen (TVB-N) content in chevon and pork during frozen storage

October 2021

The study investigated the effect of 10% aqueous extracts of ginger, garlic and onion on the quality and shelf life of frozen chevon and pork as reflected by changes in values of 2-thiobartituric acid reactive substances (2-TBARS) and total volatile basic nitrogen (TVB-N) over a 14 day storage under frozen conditions.  The pH, 2-TBARS and TVB-N of chevon and pork were measured from frozen storage at -20°C after...

Author(s): Lesten Eliez Chisomo Chatepa, Kingsley George Masamba and Jonathan Tanganyika

Spatial and regional directory of wild Auricularia species found in the forest of Southwest Nigeria

October 2021

The African forest, which was and still is the heart of biodiversity, is fast losing its bio-components due to natural disasters and human encroachment. A catalogue of the bio-components of the African forest is imminent so that conservation programs can be established to safeguard rare germplasm(s) from extinction. The population density of wild Auricularia (Mushroom) of Southwestern Nigeria was the focus of this...

Author(s): Victor S. Ekun, Clementina O. Adenipekun, Omena B. Ojuederie and Peter M. Etaware

Antioxidative and anti-aging activities of Abeliophyllum distichum Nakai extracts fermented with lactobacillus plantarum and lactobacillus brevis

October 2021

Abeliophyllum distichum Nakai is a deciduous shrub that belongs to Oleaceae Abeliophyllum and grows only in Korea. In this study, the antioxidative activity, cytotoxicity, anti-aging activity, and levels of acteoside and isoacteoside were compared between non-fermented and fermented (using Lactobacillus plantarum and Lactobacillus brevis) extracts of A. distichum Nakai. The cytotoxicity of these extracts, along with...

Author(s): KiBeom Lee, Ju Hyun Park and Yun Sung Kim  

Assessment of water requirement for rice crop at Cyabayaga wetland in Nyagatare District, Eastern Province, Rwanda

October 2021

The overall objective of this present research is assessment of rice-water requirement in Cyabayaga wetland of Nyagatare District in Rwanda. Specific objectives of this research project were to collect and analyze weather parameters, of soil physical properties, crop-water requirements and to recommend efficient water management of the study area. Research design, data collection and field observations were made during...

Author(s): Valens Nkundabashaka, Sylvestre Habimana, Rose Niyonkuru, Claude Kayijuka, Antoine Karangwa, Saidi  Rumanzi Mbaraka, Fabrice Rwalinda Musana, Eularie Mutamuliza and Marie Chantal Mutimawurugo

Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes

September 2021

Cassava (Manihot esculenta Crantz) is majorly devastated by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease (CMD), resulting in 100% yield loss. Being a clonal plant, nodal cuttings (NC) and shoot apical meristems (SAMs) are the best explants for production of disease free planting materials. In this study, NCs and SAMs were used to determine reliable indicators for successful in vitro...

Author(s): Hellen B. Apio, Titus Alicai and Emmanuel Ogwok,  

Sales environment, microbiological and biochemical quality of beef skins intended for human consumption

September 2021

The quality and composition of meat and its derivatives are influenced by many factors namely physicochemical, organoleptic factors and microbial contamination. The aim of this study was to evaluate kpakouma consumption risk through its chemical and microbiological contaminants. The methodological approach was composed of (i) observation of selling environment, (ii) pesticides and antibiotics residues quantification by...

Author(s): Haziz Sina, Kamirou Chabi-Sika, Abdou Hamidou Soule, Durand Dah-Nouvlessounon, Bawa Boya, Chantal M. Y. Viwakinnou, Virgile Ahyi, Farid Baba-Moussa, Adolphe Adjanohoun and Lamine Baba-Moussa

Adenine sulfate and glutamine enhanced shoots multiplication in Jatropha curcas L. a potent biofuel plant

September 2021

Jatropha is a drought-tolerant plant producing seed oil for the biodiesel. Limitation to the development of jatropha is unavailability of high-yielding varieties and efficient in vitro regeneration system which is required for micropropagation. In this study, in vitro regeneration system from jatropha juvenile cotyledon was established. Firstly, concentrations of hormones in the MS medium were optimized and it was found...

Author(s): Ismail A. Mohammed, Suguru Tsuchimoto, Hiroe Sakai, Naoki Wada and Kiichi Fukui

Vertical-horizontal subsurface flow hybrid constructed wetlands for municipal wastewater treatment in developing countries: A review

September 2021

The use of low-cost on-site wastewater treatment technologies, including constructed wetlands (CWs), is wide spread. Despite the purported high performance of vertical-horizontal subsurface flow (V-H SSF) hybrid CWs, data on implementation and performance in developing countries is scarce. Here, the design, operation and performance of V-H SSF hybrid CWs for treatment of municipal sewage in an effort to encourage and...

Author(s): Sylvie Muwanga Tebitendwa and Ashton Keith Cowan

Reconstruction of metabolic pathways in selected bacterial and yeast strains for the production of bio-ethylene from crude glycerol: A mini review

August 2021

The role of bioengineering in industrial processes cannot be ignored, especially in circumstances where the processes are uneconomical, demand high energy, use finite resources and emit huge amounts of carbon dioxide. Ethylene is a two-carbon unsaturated hydrocarbon that is industry's most important building block for polyester fibres, plastics, and ethylene glycol. For decades, ethylene production has relied on...

Author(s): Idan Chiyanzu

Survival rate of donkey foals: Status quo and improvement methods

August 2021

The industrial improvement of donkey-hide gelatin and the development of related products, such as donkey meat, donkey milk, and donkey-hide gelatin cake, drive the upstream donkey breeding industry. However, the shortage of donkey germplasm resources seriously restricts the further development of the donkey industry. Under the biological limitations of long breeding cycle, slow breeding, low conception rate, high...

Author(s): Yonghui Wang, Tianqi Wang, Zhengwei Zhang and Changfa Wang

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

August 2021

The prospects for using Moringa oleifera leaves extract (MLE) supplemented with different concentrations of kanwa or sodium bicarbonate (NaHCO3) as a low-cost alternative growth medium of Spirulina platensis were evaluated in a small-scale outdoor cultivation system. The present study was aimed to evaluate the potential of MLE growth medium enriched with different concentrations (4 or 8 g L-1)...

Author(s): Wamba Fotsop Oscar, Magwell Rodrigue Pierre Fils, Tchoffo Djoudjeu Kennedy, Bahoya Joseph Aime, Minyaka Emile, Tavea Marie-Frédéric, Lehman Léopold Gustave and Taffouo Victor Désiré

Phytochemical and in-vitro antioxidant properties of ethyl acetate leave extract of Dryopteris dilatata on Wistar rats

August 2021

Medicinal plants contain natural bio-active constituents in various parts called photochemical that are of therapeutic importance. This research was undertaken to determine the phytochemical constituents and in-vitro antioxidant activities of ethyl acetate extract of Dryopteris dilatata leaves (DDL). Phytochemical analysis was determined on the plant samples following established protocols. The antioxidant property of...

Author(s): Akpotu Ajirioghene, Ani Celestine, Agiopu Toby, Okeke Adaobi, Agu Francis, Ugwuchukwu Nnaemeka, Ugwudike Patrick, Eze Chukwuka, Uzoigwe Jide, Anyaeji Pamela and Ghasi Samuel

Changes in some behavioral, hematological and biochemical indices of air-breathing Clarias gariepinus [BUCHELL, 1822] exposed to pharmaceutical effluent

July 2021

This work aimed at evaluating the behavioral, biochemical and hematological effects of pharmaceutical effluent in laboratory population of Clarias gariepinus using a static renewal bioassay system. Fish specimens were collected and exposed to five (0.04, 0.06, 0.08, 0.10 and 0.12 mgL-1) sublethal concentrations of the effluent including a control experiment. Different dose dependent behavioral responses such as erratic...

Author(s): Ogundiran M. A., Ayandiran T. A., Olasunmibo A.O., Olanipeku A. S. and Okaseun O. T.

An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems

July 2021

Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from...

Author(s): Mercy Sinkiyian Kepue, Cecilia M. Mweu, David Muruu Kariuki, Linnet Kerubo, Isaac Njaci, Anne Njoroge, Leah Kago, Benard O. Mware and Sita R. Ghimire

Control of ginseng leaf black spot disease by endophytic fungi

July 2021

Ginseng plants associated with a number of fungi, several of which are reported to protect it from pathogens, thus improving plant growth. This study aimed to screen Panax ginseng leaves for endophytic fungi and to assess these fungi for their efficacy to inhibit ginseng black spot disease caused by Alternaria panax. A total of 256 endophytic fungal isolates were obtained from P. ginseng leaves. Most of the fungal...

Author(s): Chunyuan Zhou, Shiquan Xu, Xiangmin Piao, Meixia Yan, Lili Cui and Yingping Wang

Effect of light quality on in vitro germination, seedling growth and photosynthetic pigments production in wheat (Triticum aestivum L.)

July 2021

Due to its economic importance, wheat (Triticum aestivum L.) has been the subject of most breeding studies, most of which having as a starting point the in vitro cultures required for initiating different cell and tissues cultures such as protoplasts, ovules and pollen cells. All these procedures are used to obtain new varieties or hybrids regarding the improvement of different qualities such as higher productivity,...

Author(s): Cristian Felix Blidar, Remalia Semiredana Chi?, Sanda Rodica Bota, Georgeta ?erban and Oana Delia St?n??el

Effect of intercropping aerobic rice with leafy vegetables on crop growth, yield and its economic efficiency

July 2021

Aerobic rice production system provides a sustainable alternative to the traditional rice cultivation. To evaluate the agronomic and economic effect of intercropping aerobic rice with four leafy vegetables, field experiments were conducted at University of Agriculture Science Bangalore research station, India during 2017 and 2018. The experiments consisted of 9 treatments with 4 replications and, a Randomized Completely...

Author(s): Sylvestre Habimana, Saidi Rumanzi Mbaraka, Antoine Karangwa, Francois Xavier Rucamumihigo, Fabrice Rwalinda Musana, Eularie Mutamuliza, Ivan Gasangwa, Christian Nkurunziza, Jean Claude Abayisenga and Athanase Rusanganwa Cyamweshi

Efficacy of thymol and eugenol against bacterial wilt bacterium Ralstonia solanacearum

June 2021

Bacterial wilt caused by Ralstonia solanacearum is a major constraint to production of potatoes (Solanum tuberosum L). To date, there are no known conventional bactericides which provide effective control of this soil borne pathogen. This study aimed at investigating the antibacterial activity of eugenol and thymol which are the major components of thyme and clove essential oils, respectively against R. solanacearum....

Author(s): George Oluoch, Edward George Mamati, Viviene Matiru and Moses Nyongesa

Effect of VIUSID-Agro™ on the conversion of somatic embryos of coffee (Coffea arabica L.) cv. Red Caturra rojo-884

June 2021

A critical factor in the in vitro propagation protocol via somatic embryogenesis of coffee is the survival and growth of plants under ex vitro conditions. The objective of this work was to determine the effect of VIUSID Agro™ during the conversion of somatic embryos of coffee cv. Red Caturra rojo-884 in semi-controlled culture conditions. Two solutions (0.5 and 0.8 mL L-1) of the biostimulant were evaluated on in...

Author(s): Laisyn Posada-Pérez, Raúl Barbón Rodríguez, Alina Capote Pérez, Anabel Pérez Pérez, Yenny Padrón Montesino, Bulent Kukurtcu, Dion D. Daniels, Guillermo Reyes and Rafael Gómez-Kosky

Vigna radiata (mung bean) acid phosphatase is difficult to purify and may have a role as a vegetative Storage protein

June 2021

Acid phosphatase from mung bean (Vigna radiata) germinated seeds was partially purified via ammonium sulfate precipitation and fully purified via gel excision and heat denaturation, yielding a 29 kDa protein. Using 4-nitrophenylphosphate as a substrate, Vmax and Km values of 0.10 µmol/min and 0.27 mM, respectively, were obtained. The acid phosphatase was heat resistant, enhanced by the presence of Fe2+ and Mn2+...

Author(s): Hailey Elizabeth Lightle, Sarah Anne Fosco, Rachel Nicole Giles, Jordyn Marie Meekma, Nicole Marie Moore, Megan Vawn Palacio and Suzanne Elisabet Williams

Imaging from anatomic to molecular and atomic resolution scales: A review

June 2021

Imaging may be referred to as the ‘eyes of science’ as it provides scientists with highly informative multi-dimensional and multi-parameter data usually invisible to the naked eye. As instrumentation technologies and genetic engineering advances, it’s possible in modern times to observe and image highly dynamic biochemistry processes. This paper reviews positron emission tomography (PET), magnetic...

Author(s): Kamweru, Paul Kuria

CRISPR/Cas genome editing: A frontier for transforming precision cassava breeding

June 2021

Improved agricultural production of essential crops through advanced breeding is important for increasing access to nutritious food for the world's rapidly growing population, which is expected to reach 9.8 billion by 2050. Recent advancements in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9 (CRISPR/Cas9) genome editing process, which uses single-guide RNA for genome...

Author(s): Bicko Steve Juma, Cecilia Mweu, Mathew Piero and Wilton Mbinda,

Antimicrobial potentials of idiolites of endophytic fungi isolated from the leaves of Dacryodes edulis

June 2021

The growing levels of antimicrobial resistance have triggered interest in the search for alternative compounds to wage the war against it. Plants have been shown to harbor endophytic fungi that produce bioactive idiolites, which have potential lead compounds in the development of new drugs. This study evaluated the antimicrobial activity of endophytic fungi isolated from leaves of Dacryodes edulis. Endophytic fungi...

Author(s): Malachy C. Ugwu, Dave E. Ochiaja, David C. Nwobodo, Collins Chimezie and Chiamaka B. Ugwu

A rapid detection method on-site for Zygosaccharomyces based on loop-mediated isothermal amplification (LAMP) combined with a lateral flow dipstick (LFD)

May 2021

The Zygosaccharomyces is notorious for its remarkable spoilage characteristics. In the present study, the visual and rapid identification of the genus Zygosaccharomyces was performed by a loop-mediated isothermal amplification (LAMP) assay using specific primers in Mini Dry Bath within 30 min at 65°C followed by a lateral flow dipstick (LFD) detection. The sensitivity evaluation revealed LAMP-LFD assay with...

Author(s): Yang Liu≠, Jun Wu≠, Li-Qing Chen, Ya Shi, Hong-Xia Zhao, Wei Chen, Hong Li and Lu-Ping He,

Synthesis, characterization and antibacterial activity of AgNPS mediated by Ocimum sanctum

May 2021

This research focused on isolation of Silver Nano Particles (AgNPs) using leaf filtrate of Ocimum sanctum and evaluation of their antibacterial action against Bacillus aureus, Staphylococcus aureus and Escherichia coli. It is known that plant extracts act as reducing agents, stabilizers and as capping agents. The O. sanctum plant was specifically selected due to its medicinal properties and abundant availability. The...

Author(s): A. V. Narasimha Swamy and R. Srinu Venkat Rao

Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans

May 2021

Angular leaf spot (ALS) is one of the most severe diseases of common bean. However, there is as yet no standardization of the phenological stage, inoculum concentration, diagrammatic scale (DS), and day of assessment for screening ALS resistance in bean lines. The present study aimed to evaluate the potential for assessment of ALS severity in common beans using the Leaf Doctor app and inoculation at the V2 phenological...

Author(s): Caléo Panhoca de Almeida, Gabriel Francesco Janini Bonfante, Jean Fausto de Carvalho Paulino, Bianca Cristina de Deus, Flávia Rodrigues Alves Patrício, Sérgio Augusto Morais Carbonell, Alisson Fernando Chiorato and Luciana Lasry Benchimol-Reis

Optimization of growth media for maximal production of insecticidal vegetative protein (vip3A) from Bacillus thuringiensis and its activity against black cutworm (Agrotis ipsilon)

May 2021

Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust. B. thuringiensis has been used for decades as biological control for pests of economical and medical importance. The aim of this study was to test different growth media for the optimal production of vegetative insecticidal protein (Vip3) by B. thuringiensis recovered from the local environment. The local B. thuringiensis...

Author(s): Fatimah Alshehrei, Doaa K. El-Ghareeb, Ashraf El Baz, Sameer Qari, Hanafy Hamza, Hussein H. Abulreesh, Hesham A. Malak, Rehab Elmougy and Gamal E. H. Osman,

Quantification and characterization of faecal sludge produced in Kara

May 2021

In Kara (Togo), as in most major sub-Saharan African cities, there is very little sewerage and autonomous systems are the most commonly used. These autonomous systems produce faecal sludge that must be drained and treated adequately in order to preserve the health of the population and protect the environment. Indeed, the risks are real, given the use of fresh faecal sludge in agriculture and the potential dangers of...

Author(s): Piyabalo KODOM, Edem Komi KOLEDZI, Nitale M’Balikine KROU, Kwamivi N. SEGBEAYA and Bouwèdèo TOI BISSANG

Production of truncated peptide (cellobiohydrolase Cel6A) by Trichoderma reesei expressed in Escherichia coli

May 2021

Enzymatic cellulose hydrolysis is an important step for the production of second-generation biofuels. The filamentous fungus Trichoderma reesei is among the most important organisms for obtaining cellulolytic enzymes. The Cel6A (CBH II) cellulase from T. ressei plays an important role in cellulose hydrolysis and acts on the non-reducing end of cellulose, in contrast to Cel7B (CBH I), which acts on the reducing end of...

Author(s): Miriam Shirley Tellez Calzada, Juan Antonio Rojas Contreras, Jesus Bernardo Paez Lerma, Nicolas Oscar Soto Cruz and Javier López Miranda

Synthesis, physicochemical and antimicrobial properties of rhenium (I) tricarbonyl complexes of isatin Schiff bases

May 2021

The Schiff base of isatin and sulphanilamide (L1; C14H11N3O3S) was prepared and reacted with Re(CO)5X (X = Cl and Br) in toluene to give [Re(C14H10N2O)(CO)3X] (X = Cl, 1 and X = Br, 2). The prepared Schiff base of isatin and 4-methoxyaniline (L2; C15H12N2O2) was reacted with Re(CO)5X in toluene to give [Re(C14H10N2O)(CO)3X], where X = Cl, 3 and X = Br, 4. L2 and Re(CO)5Br were refluxed in dry toluene under nitrogen and...

Author(s): Adebomi A. Ikotun, Abimbola A. Owoseni and Gabriel O. Egharevba  

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