African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12172

AJB Articles

June 2017

Heavy metal contamination in soils from a municipal landfill, surrounded by banana plantation in the eastern flank of Mount Cameroon

Municipal solid waste generated in our cities, with an unprecedented population growth, has resulted in degrading environmental quality, thus a major problem for policy makers. The extent of Pb, Cu, Zn, Cd, Hg and Zn contamination in soils of the studied area, where vegetables were grown, using metal contamination factor (CF) and pollution load index were assessed. The concentrations of heavy metals studied were in the...

Author(s): Fonge, B. A., Nkoleka, E. N., Asong, F. Z., Ajonina, S. A. and Che, V. B.

June 2017

Improvement of color and increase in anthocyanin content of ‘Niagara Rosada’ grapes with application of abscisic acid

This study aimed to assess the application effect of abscisic acid (S-ABA) on color, and total anthocyanin and phenolic compound contents of the ‘Niagara Rosada’ grapes. The experiment was carried out in a commercial vineyard located in Jales, SP, Brazil, during 2014/2015. The randomized block design was used with four treatments and five replications. The treatments consisted of applying different...

Author(s): Marco Antonio Tecchio, Francisco José Domingues Neto, Adilson Pimentel Junior, Marlon Jocimar Rodrigues da Silva, Sérgio Ruffo Roberto and Ronny Clayton Smarsi

June 2017

DEXseq and Cuffdiff approaches weighing differential spliced genes exons modulation in estrogen receptor β (Erβ) breast cancer cells

While the alternative transcription and splicing mechanisms have long been known for some genes like oncogenes, their prevalence in almost all multi-exon genes has been recently realized with the increasing application of high-throughput experimental methods, named Next Generation RNA Sequencing (NGS). Henceforth, understanding the regulation of these processes in comparisons between cell types and cancer requests,...

Author(s): Noel Dougba Dago, Nafan Diarrassouba, Inza Jesus Fofana, Jean-Luc Aboya Moroh, Hermann-Désiré Lallié, Didier Martial Yao Saraka, Oléfongo Dagnogo, Souleymane Silué, Armel Herve Nwabo Kamdje, Lamine Baba-Moussa and Alessandro Weisz

June 2017

Production potential and chemical composition of elephant grass (Pennisetum purpureum Schum.) at different ages for energy purposes

The elephant grass has gained prominence as one of the main forage species used for biomass production. Therefore, the aim of this study was to identify elephant grass genotypes with high energy biomass production potential by evaluating morpho-agronomic and biomass quality. The following traits were evaluated in this study: dry matter yield (DMY), percentage of whole-plant dry matter (%DM), percentage of neutral...

Author(s): Ana Kesia Faria Vidal, Tatiane da Costa Barbé, Rogério Figueiredo Daher, Janeo Eustáquio Almeida Filho, Roberta Samara Nunes de Lima, Rafael Souza Freitas, Drieli Aparecida Rossi, Érik da Silva Oliveira, Bruna Rafaela da Silva Menezes, Geovana Cremonini Entringer, Wanessa Francesconi Stida Peixoto and Sabrina Cassaro

June 2017

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

Xylooligosaccharides (XOS) are sugar oligomers made up of xylose units with the chain length of 2 to 10 and are considered non-digestible food ingredients. It is mainly produced from xylan hydrolysis. XOS have a characteristic of prebiotic by promoting the growth of probiotic organisms. They have various physiological effects such as reducing cholesterol, maintaining the gastrointestinal health and improving the...

Author(s): Praveen Kumar Gupta, Pushpa Agrawal and Prabha Hegde

June 2017

Immunocapture and simple-direct-tube-reverse transcriptase polymerase chain reaction (RT-PCR) for detection of Rice yellow mottle virus

This study aimed at optimizing the immunocapture (IC) and simple-direct-tube (SDT) -reverse transcriptase polymerase chain reaction (RT-PCR) techniques for detection of Rice yellow mottle virus (RYMV) in order to avoid the extraction of high quality RNA required for molecular methods and avoid costs involved. Rice yellow mottle virus strains and phylotypes were obtained from infected rice leaf samples collected from...

Author(s): Judith Hubert, Herman John Faraji Lyimo, Ashura Luzi-Kihupi and Ernest Rashid Mbega

June 2017

Phytochemical screening and amino acids analysis of mushrooms from Burkina Faso

Mushrooms play an important social, economic and ecological role in the world. In this study, the proximate compositions, minerals and amino acids contents of Russula alveolata, Russula cf. compressa, Russula flavobrunnea var. aurantioflava and Russula ochrocephala from Burkina Faso were investigated. The chemical analysis showed the presence of volatile oil, sterols and triterpenes, carotenoids, saponosides, reducing...

Author(s): Elise Sanon, Jean Claude W. Ouédraogo, Sylvain Ilboudo, Marie K. Laure Guissou, Pierre I. Guissou and Philippe Sankara

June 2017

Establishment, multiplication, rooting and acclimatization of Cabralea canjerana (Vell.) Martius

Seedling production of canjerana (Cabralea canjerana (Vell.) Martius) has been limited by difficulty in germination, caused by recalcitrant behavior of their seeds. The objective of this study was to develop an efficient protocol for conservation and production of canjerana micropropagated plantlets. Seeds of canjerana were disinfected with 0, 2.5, 5.0, 7.5 and 10% of NaOCl solution, at immersion times of 10, 20 and 30...

Author(s): Eliseo Salvatierra Gimenes, Nathalia Pimentel, James Hancock and Dilson Antônio Bisognin

June 2017

Optimised cetyltrimethylammonium bromide (CTAB) DNA extraction method of plant leaf with high polysaccharide and polyphenolic compounds for downstream reliable molecular analyses

Yam (Dioscorea species) and banana (Musa species) leaf sample contain high levels of polysaccharide and polyphenolic compounds. Extraction of good quality DNA from such leaves is usually problematic. Therefore, there is a need to extract good quality DNA in order to perform downstream DNA analysis, especially for the detection of viruses due to their low titration in some infected leaves. The cetyltrimethylammonium...

Author(s): Turaki A. A.,, Ahmad B., Magaji U. F., Abdulrazak U. K., Yusuf B. A. and Hamza A. B.,

June 2017

Agrobacterium-mediated genetic transformation of Fonio (Digitaria exilis (L.) Stapf)

Fonio (Digitaria exilis) is a crop grown in Africa for its excellent culinary and nutritional properties and is a valuable source of income for smallholder farmers. Its improvement has long been neglected by scientists because the crop was one of the lost crops of Africa. Recent advances in Fonio research are centered on its evolution, nutritional and economic values. The lack of a transformation and other biotechnology...

Author(s): Valentine Otang Ntui, Edak Aniedi Uyoh, Ikuo Nakamura and Masahiro Mii

June 2017

Evaluation of sesame (Sesamum indicum L.) germplasm collection of Tamil Nadu for α-linolenic acid, sesamin and sesamol content

A germplasm collection of Tamil Nadu comprising of nine varieties and six landraces were analyzed for their ω3 fatty acid content, lignans such as sesamin and sesamol. The percentage of ALA ranges from 0.1 to 0.55%. A wide variation was observed in sesamol content. The highest sesamol content of 0.547 mg/g was found in a variety SVPR1. The sesamin content ranged between 2.03 and 6.45 mg/g. A negative relationship...

Author(s): Muthulakshmi Chellamuthu, Pavithra Sekar and Selvi Subramanian

June 2017

Morphophysiology of peppermint irrigated with salt water and bovine biofertilizer

Peppermint is a medicinal plant grown worldwide, but it has not been extensively studied, especially the use of saline water for its cultivation. In this sense, the objective of this study is to evaluate the effect of saline waters on peppermint cultivation under the application of bovine biofertilizer. The experiment was carried out from September 2015 to December 2015 in a greenhouse of the Center of Human and...

Author(s): Mario Leno Martins Veras, José Sebastião de Melo Filho, Lunara de Sousa Alves, Kelina Bernardo Silva, Evandro Franklin de Mesquita, Raimundo Andrade, Karialane da Silva Belarmino, Mayara Andrade de Souza, Danrlei Varela Ribeiro, Alvaro Carlos Gonçalves Neto, Uilma Laurentino da Silva, Juliara dos Santos Silva and Ayonna Savana  Fernandes Linhares 

May 2017

Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations

Polymorphism analysis of the Kisspeptin (KISS1) gene and its association with litter size were conducted in two (Gondar and Woyto Guji) Ethiopian indigenous goat populations. Three new pairs of primers were designed for touchdown PCR condition.  A total of 173 and 242 Sanger sequences were employed for further analysis of exon1 (1210 bp) and exon2 (325 bp) regions of the KISS1 gene, respectively. In addition, the...

Author(s): Getinet Mekuriaw,, Joram M. Mwacharo, Tadelle Dessie, Okeyo Mwai, Appolinaire Djikeng, Sarah Osama, Grum Gebreyesus, Alayu Kidane, Solomon Abegaz and Kassahun Tesfaye

May 2017

Establishing sterilization protocol and shoot induction medium for in vitro regeneration of sweetbriar (Rosa rubiginosa L.)

Rosa rubiginosa L. is popular in Lesotho where rural communities harvest the rosehips for sale to the local companies which process them into various products. There is, however, a challenge in establishing plantations of the species due to deep seed dormancy and so difficulty in germination. In this study, nodal explants of R. rubiginosa L. were sterilized with 20 g·L-1 Ridomil Gold MZ (RGM) and cultured on...

Author(s): Miloane Meshack Mokhobo, Remmy Wekesa Kasili and Amos Emitati Alakonya

May 2017

Biodiesel production from marine microalgae Nannochloropsis gaditana by in situ transesterification process

Microalgae is one of the best sources of renewable energy production, such as biofuels. The production of biodiesel from microalgae has several advantages, including the high productivity of lipid and the possibility of cultivating them on marginal land. One of the challanges in using microalgae for biodiesel production is the complexities process of lipids extraction by organic solvents followed by transesterification....

Author(s): BENZIDANE Dehiba, BABA HAMED Mohamed Bey and ABI-AYAD Sidi-Mohammed El-Amine

May 2017

Truffle mediated (Terfezia claveryi) synthesis of silver nanoparticles and its potential cytotoxicity in human breast cancer cells (MCF-7)

Terfezia claveryi is a species that belongs to the genera of Terfeziaceae or desert truffles, which is a family of truffles. In the present study, silver nanoparticles were synthesized from aqueous extract of T. claveryi which are in the range of 25 to 60 nm. The synthesized nanoparticles were characterized by ultraviolet-visible (UV-Vis) spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray diffraction...

Author(s): Habeeb Khadri, Yousef H. Aldebasi and Khateef Riazunnisa

May 2017

Genetic and morphological diversity among sweet potato (Ipomoea batatas (L) Lam.) accessions from different geographical areas in Malawi

An understanding of morphological and genetic diversity of sweet potato landraces is fundamental to any breeding program in a country. Fifty-nine sweet potato accessions from three eco-geographical populations of northern, south eastern and southern Malawi were examined using ten Simple Sequence Repeats (SSR) loci and seven International Board for Plant Genetic Resources (IBPGR) descriptors of sweet potato. The study...

Author(s): Felistus Chipungu, Wisdom Changadeya, Aggrey Ambali, John Saka, Nzola Mahungu and Jonathan Mkumbira

May 2017

Postpartum serum biochemical profile of Sudanese cystic ovarian crossbred dairy cattle

Cystic ovarian disease (COD) is an ovarian dysfunction in cows resulting in a serious economic loss in the dairy industry. This study was conducted to examine the hemoglobin (Hb) concentration, serum total protein (TP), phosphorus (P), copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) levels of Sudanese crossbred (Friesian x Kenana) cows with COD in semi-closed condition. Forty-five dairy cows were divided into two...

Author(s): Nasser Mohammed Osman, Imadeldin Elfaki, Faisal Omer Ahmed and Abdelrahim Hommeida

May 2017

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

Pseudomonas aeruginosa is intrinsically resistant to several antimicrobial drugs classes. Various extended-spectrum beta lactamases (ESBL) types have been found in P. aeruginosa such as Pseudomonas extended resistance (PER) and Guiana extended-spectrum (GES) enzymes. The study aimed to evaluate the susceptibility of the ESBL producing P. aeruginosa strains that express blaGES and blaPER genes to commonly used...

Author(s): Omar B. Ahmed and Atif H. Asghar

May 2017

Genetic diversity and population structure of Ethiopian finger millet (Eleusine coracana (L.) Gaertn) genotypes using inter simple sequence repeat (ISSR) markers

Eleusine coracana is an annual allotetraploid (2n=4x=36) that belongs to grass family, Poaceae. This research aimed to investigate level of genetic diversity of 80 accessions using ISSR markers. DNA was extracted from a bulk of three plants per accession using a modified CTAB method. Six ISSR primers amplified a total of 45 clear and reproducible bands. The total genetic diversity (H) and Shannon’s diversity...

Author(s): Haftom Brhane, Teklehaimanot Haileselassie and Kassahun Tesfaye

May 2017

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated...

Author(s): Walid Ahmed Lotfy, Neveen Mohamed Abd-El-Karim, Ebaa Ebrahim El-Sharouny and Ehab Ragheb El-Helow

May 2017

Diversity of Aspergillus isolates and selection of an isolate with high β-fructofuranosidase activity that is native to the Peruvian coast

In order to obtain a native isolate with high β-fructofuranosidase activity (FFase), a preliminary selection of 44 isolates, predominantly formed by Aspergillus niger (72%), was performed among  167 isolates of Aspergillus native to the Peruvian coast (Casa grande, Cartavio, Paramonga and Tacama). In addition, genetic diversity was studied using molecular markers, Inter Simple Sequence Repeats (ISSR). The...

Author(s): Boris Gutarra Castillo, Marina Díez-Municio, Susan Canchaya Garcia and Gaby Calixto Zacarias, Juan Cisneros de la Cruz and F Javier Moreno

May 2017

Myracrondruon urundeuva Allemão: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and...

Author(s): André Luiz Lima Menezes Santos, José Davi Prado Lima, Clivia Rolemberg Andrade, Deisylaine Maria Santos, Antonio Santos Dias, Pietra Alexia Lima Santos, Rafaela Karolina Vianna Nunes, José Evaldo Rodrigues Menezes Filho, José Nilson Andrade Santos, Carla Maria Lins Vasconcellos, Andrea Yu Kwan Shan, Brancilene Santos Araujo and Charles Santos Estevam

May 2017

Chromosomal and plasmid mediated degradation of crude oil by Bacillus coagulans, Citrobacter koseri and Serratia ficaria isolated from the soil

In this work, the degradative abilities of some bacteria isolated from the soil were studied. Five distinct isolates were subjected to substrate susceptibility test to determine their potential degradative abilities in the presence of different hydrocarbons. The best three crude oil degraders were selected and their identities were further established using API technique. Furthermore, the extent of each degradative...

Author(s): Fagbemi O. Kehinde and Sanusi A. Isaac

May 2017

Effect of different concentrations of bush passion fruit pulp and temperature in the production of beer

Among many species of passion fruit, the bush passion fruit (Passiflora cincinnata Mast.) is a wild, edible fruit, of exotic flavor and has good acceptability for consumption. This study aimed to determine the physico-chemical characteristics of the bush passion fruit pulp, and to produce and characterize physico- chemically the ale beer made with bush passion fruit as a malt adjunct, using different percentages (10,...

Author(s): Verena Fiscina de Melo, Geiza Suzart Araújo, José Ailton Conceição Bispo, Vanildo Luiz Del Bianchi and Giovani Brandão Mafra de Carvalho

May 2017

Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province

The objective of this research was to obtain quantitative growth hormone gene polymorphism association between growth hormone gene genotype with quantitative characteristics of the thin-tail sheep in the highlands and lowlands of Jambi Province. Two phases of research were done on the field and in the laboratory. Field research which was conducted to obtain the quantitative characteristics data includes; withers height...

Author(s): Depison, Sarbaini Anwar, Jamsari, Arnim and Yurnalis

May 2017

Exploration of Sri Lankan soil fungi for biocontrol properties

Sri Lankan soil is a rich source of fungi with new strains that are not well explored to date. In the present study, a total of 83 soil fungi were isolated from different locations of Sri Lanka using chitin and β-1,3-glucan selective media. Of the 83 isolates, 22 isolates showed high biocontrol activities against seven selected plant pathogenic fungi in dual culture plate assay. These 22 isolates were further...

Author(s): Herath H. H. M. A. U., Wijesundera R. L. C., Chandrasekharan N. V. and Wijesundera W. S. S.

May 2017

Computer simulation of benzene, toluene and p-xylene adsorption onto activated carbon

In this work, the adsorption of benzene, toluene and p-xylene on activated carbon was verified through a computer simulation. A carbon model was used as the work basis. Several steps were completed from the modeling of the carbon structures and pollutants to simulations of molecular dynamics. After analyzing the results, it was found that the groups: ether, lactone and carbonyl (ketone) present in the carbon structure...

Author(s): Wanessa Almeida da Costa, Carlyle Ribeiro Lima, Frederico Guilherme Santana da Silva Filho, Mozaniel Santana de Oliveira, Renato Macedo Cordeiro, Raul Nunes de Carvalho Junior, Marlice Cruz Martelli and Davi do Socorro Barros Brasil

May 2017

The influence of potassium dichromate on growth and survival of the vermicomposting earthworm Eisenia foetida

The present research evaluated the redox ability of Cr6+ in the earthworm Eisenia foetida, and its effects on growth and survival. An assay was conducted with an increasing doses of K2Cr2O7 (0, 200, 400, 800, 1000, and 1200 mg K2Cr2O7 kg-1 substrate) applied to a vermicomposting system which was inoculated with 30 earthworms per chamber. Earthworm growth, estimated by weight, showed a quadratic response (p = 0.03) with...

Author(s): Martha Angélica Ramírez Tabche, Juan José Peña Cabriales, María Maldonado Vega and José Antonio Vera-Núñez

May 2017

Effect of cooking on tannin and phytate content in different bean (Phaseolus vulgaris) varieties grown in Tanzania

Phytates and tannins are present in varying proportions in legume based foods. Investigation on the effect of cooking on tannins and phytates was carried out on thirty eight raw and cooked bean (Phaseolus vulgaris) varieties. Tannins were assayed by vanillin-hydrochloric acid method, while phytates were determined by a method developed by Haugh and Lantzsch. There was a very high significant difference (P≤0.00001) in...

Author(s): Mamiro, P. S., Mwanri, A. W., Mongi, R. J., Chivaghula, T. J., Nyagaya, M. and Ntwenya, J.

May 2017

Genomic DNA isolation method from fresh wheat leaf samples without liquid nitrogen

The advent and application of molecular markers technology enabled dissection of genomic regions underling important agronomic traits in crop plants. Isolation of sufficient quantity and good quality DNA from a large number of plant samples is a major challenge in most genetic and genomic analyses. Wheat is the world’s third most important food security crop on which most molecular breeding and genetic engineering...

Author(s): Tilahun Mekonnen, Krishnamurthy Sathelly, Manju Sharma, Kassahun Tesfaye and Tanushri Kaul

May 2017

Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil

Heavy metals are transition elements whose presence at mild doses in water bodies is deadly. Most effectively harnessed technique of their remediation is the microbial approach, also known as bio-remediation. This study is hereby aimed to investigate the potential of Klebsiella species isolated from diesel polluted soil in bioremediating the heavy metals present in effluent water.  A sample of diesel polluted soil...

Author(s): Aransiola E. F., Ige O. A., Ehinmitola E. O. and Layokun S. K.

May 2017

Optimized production of lipase from Bacillus subtilis PCSIRNL-39

Lipases catalyze the hydrolysis of long chain triglycerides. Microbial lipases are receiving much attention because of their industrial potential in the chemical, pharmaceutical, medical, cosmetic, biosurfactant synthesis, leather industries, mutation, agrochemicals and paper manufacturing industries. This article presents the isolation of maximum lipase producing bacteria and the optimization of different conditions...

Author(s): Haniya Mazhar, Naaz Abbas, Sakhawat Ali, Amir Sohail, Zahid Hussain and Syed Shahid Ali

May 2017

Persimmon leaf and seed powders could enhance nutritional value and acceptance of green tea

Acceptance of green tea, a health promoting functional food material, could be increased if its flavor is improved. Persimmon seeds and leaves are likely potential options for green tea improvement since they contain a large number of volatile flavor compounds. In the present study, amino acids, volatile flavor compounds, and antioxidant potentials of persimmon tea (PT) prepared with green tea were investigated. PT...

Author(s): Il-Doo Kim, Sanjeev Kumar Dhungana, Hye-Ryun Kim, Young-Joon Choi and Dong-Hyun Shin

May 2017

Traditional production technology, consumption and quality attributes of toubani: A ready-to-eat legume food from West Africa

Toubani is a traditional ready-to-eat legume food, consumed as staple food with stews. This study investigated the socio-cultural profile of stakeholders, the traditional processing techniques and sensory quality attributes. For this purpose, a survey was carried out in two municipalities using a questionnaire managed to stakeholders. The results showed that toubani production is exclusively undertaken by women while...

Author(s): Franck Hongbété, Abdoul-Kader Tidjani and Janvier Mêlégnonfan Kindossi,

May 2017

Juice clarification with tannases from Aspergillus carneus URM5577 produced by solid-state fermentation using Terminalia catappa L. leaves

Tannase is an inducible extracellular enzyme produced by filamentous fungi, yeasts, and bacteria by solid-state fermentation (SSF) or submerged fermentation (SmF). Among the filamentous fungi, Aspergillus and Penicillium are recognized as the most efficient producers of tannase. The aims of this study were to evaluate the production of tannase under SSF by isolation from Aspergillus and Penicillium preserved in the...

Author(s): Vanilla Mergulhão Alves da Silva, Roberta Cruz, Julyanna Cordoville Fonseca, Cristina Maria de Souza-Motta, Amanda Reges de Sena and Keila Aparecida Moreira

May 2017

Common bean germplasm resistant to races 73 and 2047 of Colletotrichum lindemuthianum

The present work aims to identify common bean accessions resistant to anthracnose by incompatibility reaction evaluations to races 73 and 2047 of C. lindemuthianum, associated with using of SF101072 and SAS13950 molecular markers linked, respectively, to gene Co-10 and allele Co-42. A total of 75 common bean accessions from Common Bean Germplasm Bank (CBGB) of Núcleo de Pesquisa Aplicada à Agricultura...

Author(s): Luana Mieko Darben, Adriana Gonela, Haroldo Tavares Elias, Claudete Rosa da Silva, Heloisa Helena Pastre and Maria Celeste Gonçalves-Vidigal

May 2017

Genetics and environmental trends in growth performance of Horro (Zebu) and crosses of Holstein Friesian and Jersey cattle breeds

Phenotypic, genetics and environmental trends of birth (BW), weaning (WW) and one year weight (YW), and average daily gain were considered on data collected from Horro cattle and their crosses during the year 1980-2008. Estimated breeding value (EBV) of all animals were generated from a univariate model analysis by the general linear mixed model by residual maximum likelihood (ASREML) while least square means for annual...

Author(s): Habtamu Abera

May 2017

Silencing of the rift valley fever virus s-genome segment transcripts using RNA interference in Sf21 insect cells

Rift Valley fever (RVF) is a mosquito-borne disease that affects humans and livestock. It results in economically disastrous livestock deaths in Africa. The causative agent is RVF virus (RVFV), which possesses a tripartite negative-sense, single-stranded RNA genome, with large (L), medium (M) and small (S) segments. The lack of effective vaccines and anti-RVFV therapies motivates concerted efforts in search for safe and...

Author(s): Juliette Rose Ongus, Evans Kiplangat Rono and Fred Alexander Wafula Wamunyokoli

May 2017

Cyclical changes in the histology of the gonads (ovary and testes) of African pike, Hepsetus odoe

Morphology, stages of maturation and histology of gonads of African pike, Hepsetus odoe from Ado Ekiti Reservoir were studied from August 2014 to July 2015 in order to establish its reproductive biology. Out of 685 specimens examined 354 were males and 331 were females giving a sex ratio of 1:1.1 (female:male). There was no significant difference (P>0.05) in the sex ratio. The gonads were in pairs. All the gonad...

Author(s): Idowu, E. O.

May 2017

The African black soap from Elaeis guineensis (Palm kernel oil) and Theobroma cacao (Cocoa) and its transition metal complexes

African black soap is an indigenous African organic soap formed by esterification. This was prepared by reacting palm kernel oil and the filtrate of cocoa pod ash. Chemical analyses revealed the moisture content was 26% (w/w), total fatty matter (TFM) was 44.75% (w/w), total fatty alkaline (TFA) was 0.22% (w/w), total alkaline (TA) was 11.78% (w/w) and pH was 10. The metal complexes were formed by the reaction of the...

Author(s): Adebomi A. Ikotun, Oladipupo O. Awosika and Mary A. Oladipo

May 2017

Metabolic engineering of Corynebacterium glutamicum to enhance L-leucine production

This work aimed to develop an efficient L-leucine industrial production strain of Corynebacterium glutamicum by using metabolic engineering. A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase (IPMS) that carries three amino acid exchanges (R529H, G532D and L535V) from the mutant strain C. glutamicum ML1-9 which was obtained by screening for...

Author(s): Huang Qingeng, Liang Ling, Wu Weibin, Wu Songgang and Huang Jianzhong

May 2017

Fourier transform infrared spectroscopic analysis of maize (Zea mays) subjected to progressive drought reveals involvement of lipids, amides and carbohydrates

Changing climate means that there will be more episodes of drought, especially in arid regions. Drought stress is an important environmental factor that reduces crop productivity. Despite the vast amount of literature on drought stress in plants, there are still many uncertainties concerning the molecular responses under water deficit. In particular, if we wish to breed plants that are able to tolerate stress,...

Author(s): Chukwuma C. Ogbaga, Matthew A. E. Miller, Habib-ur-Rehman Athar and Giles N. Johnson

May 2017

The nitrogen-fixing Bradyrhizobium elkanii significantly stimulates root development and pullout resistance of Acacia confusa

Reforestation of native Acacia confusa Merr. on landslide areas in Taiwan is important for agroforestry and soil conservation. To ensure high survival and growth vigor, A. confusa seedlings must develop a strong root system. Inoculating of acacia with symbiotic nitrogen-fixing bacteria (NFB) may ameliorate the problems associated with soil nutrient deficiency on landslide sites. In this study, under plastic house...

Author(s): Jung-Tai Lee, Sung-Ming Tsai and Chung-Hung Lin

May 2017

Screening of exopolysaccharide-producing coccal lactic acid bacteria isolated from camel milk and red meat of Algeria

The analysis of coccal lactic acid bacteria (CLAB) strains for their competence in polysaccharide production has been given proper attention as texturing and thickening agents. The characterization of these strains has been performed from two natural environments (camel milk and red meat). All the isolates were evaluated for exopolysaccharides (EPS) production on certain solid medium and ruthenium red milk agar plate....

Author(s): Imène Kersani, Halima Zadi-Karam and Nour-Eddine Karam

May 2017

Proximate composition, microbiological safety and heavy metal contaminations of garri sold in Benue, North-Central Nigeria

This study investigated the proximate composition, microbial safety and heavy metal contaminations of garri: a cassava ready-to-eat food product. Garri is prepared from Manihot esculenta Crantz tubers by peeling, washing and grating of the tubers and fermentation. Production, storage and selling locations could be necessary predictors of the quality and safety of garri. A total of two hundred and sixteen (n=216) samples...

Author(s): Innocent Okonkwo Ogbonna, Blessing Ifeoma Agbowu and Felix Agbo

May 2017

Efficacy evaluation of spinosad bioinsecticide capsules suspension for the control of Helicoverpa armigera

Helicoverpa armigera caterpillar causes serious economic crop losses in Brazil, mainly in the corn, cotton and soybean farming. The aim of this study was to compare the efficiency of bioinsecticide Spinosad in the form of suspension capsules and emulsifiable concentrate in the dosage of 48 g i.a. ha-1 for control of H. armigera. The experiment was conducted in three different groups treated separately with emulsifiable...

Author(s): Francinea Souza, Leila T. Maranho and Ligia A. C. Cardoso

April 2017

Bioaccumulation of zinc in Rana tigrina in different aquatic habitats

Some creatures are very sensitive to water borne pollutants and can be used as useful bio-indicators of water pollutions. The present study used these bio-indicators to determine the status regarding health of freshwater habitats. The study was carried out to quantify Zn in kidney and liver of Rana tigrina from different aquatic habitats (canal, fish pond and sewage water) by atomic absorption spectrometry (AAS). Liver...

Author(s): Muhammad Nasir, Tariq Mahmood Ansari, Ghulam Yasin, Muhammad Shoaib, Muhammad Ahmad Mudassir and Aleem Ahmad Khan

April 2017

Changes in phenol metabolism of minimally processed ‘baby cassava’ under different temperatures: An alternative to commercialization

This study aimed to evaluate the changes in the phenolic oxidation mediated by polyphenol oxidase (PPO) and peroxidase (POD) in the quality of minimally processed ‘baby cassava’, during the first hours and days of conservation at different temperatures. Pieces of roots of cassava cv. Recife were minimally processed into ‘baby cassava’ form and kept at 5 ± 2 and 25 ± 2°C for 12h...

Author(s): Fred Augusto Lourêdo de Brito, Rúbia Kécia Marins Maia, Luiz Ferreira Coelho Júnior, Daniel Gomes Coelho, Marcos Ribeiro da Silva Vieira, Sérgio Luiz Ferreira da Silva and Adriano do Nascimento Simões

April 2017

High-performance liquid chromatography-diode-array detector (HPLC-DAD) analysis and evaluation of antioxidant and photoprotective activities of extracts from seeds of Simira gardneriana M. R. V. Barbosa and Peixoto (Rubiaceae)

Simira gardneriana is a Rubiaceae species commonly found in the Brazilian Northeast region, presented several therapeutic and biotechnological applications. In this paper, the antioxidant and photoprotective properties of extracts from the seeds of S. gardneriana were highlighted. The antioxidant activity of ethanol and methanol extracts (Si-EtOH and Si-MeOH, respectively) was determined, using...

Author(s): Christiane Adrielly Alves Ferraz, Raimundo Gonçalves de Oliveira-Júnior, Ana Paula de Oliveira, Sarah Raquel Gomes de Lima-Saraiva, Grasielly Rocha Souza, José Alves de Siqueira-Filho, Larissa Araújo Rolim, Pedro José Rolim-Neto, Jackson Roberto Guedes da Silva Almeida and Xirley Pereira Nunes

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