African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

AJB Articles

July 2017

Effect of resin from Commiphora swynnertonii on white blood cell and selected haematopoietic organs in albino mice (Mus musculus)

Commiphora swynnertonii is among the most commonly used medicinal plants by pastoralist communities especially in northern regions of Tanzania. The effect of resin from this plant on white blood cells (WBC) and haematopoietic organs was studied using albino mice. Sixty adult mice were randomly assigned into four groups (n = 15). G1 acted as control whereas G2, G3 and G4 received oral doses of 50, 100 and 200 mg resin...

Author(s): Bakari G. G., Ceaser G., Max R. A. and Mbasa G. K.

July 2017

Characterization of cassava starch films plasticized with glycerol and strengthened with nanocellulose from green coconut fibers

The aim of this study was to obtain and characterize biodegradable films of cassava starch plasticized with glycerol and reinforced with nanocellulose from coconut fibers. The mechanical and physical-chemical properties of the nano-biocomposites films obtained were evaluated. The method used to investigate the viability of incorporating coconut nanocellulose in films was applied through a statistical design of the...

Author(s): Bruna Aparecida Souza Machado, João Henrique de Oliveira Reis, Lindaiá Santos Cruz, Ingrid Lessa Leal,  Josiane Dantas Viana Barbosa, Joyce Batista Azevedo and Janice Izabel Druzian

July 2017

Quantitative trait loci for resistance to spotted and African maize stem borers (Chilo partellus and Busseola fusca) in a tropical maize (Zea mays L.) population

Maize (Zea mays L.) is the staple food in Kenya, and mapping the qualitative trait loci (QTL) associated with resistance to maize stem borer pest is important towards marker assisted breeding for this quantitative trait. The objective of this study was to identify any QTL associated with resistance to Chilo partellus and Busseola fusca, the two important stem borer pests in maize production in Kenya. A total of 203 F2:3...

Author(s): Munyiri, S. W. and Mugo, S. N.

July 2017

Silver nanoparticles biosynthesis and impregnation in cellulose acetate membrane for anti-yeast therapy

Silver nanoparticles (AgNPs) are nanoforms that express higher antimicrobial potential due to their shape and reduced size. The use of fungi to mediate the synthesis of AgNPs has increased the interest of scientists because of their rapid growth, large-scale cultivation and secretion of non-toxic molecules. The aim of this study was to synthesize AgNPs mediated by Aspergillus oryzae DPUA 1624 and evaluate the...

Author(s): Taciana A. Silva, Patrícia F. Andrade, Karen Segala, Larissa S. C. Silva, Larissa P. Silva, Silvia V. G. Nista, Lucia H. I. Mei, Nelson Durán, and Maria F. S. Teixeira

July 2017

Generation of gua1 deletion using polymerase chain reaction (PCR)-mediated gene disruption method in fission yeast, Schizosaccharomyces pombe

Gua1 gene in Schizosaccharomyces pombe encodes inosine 5′-monophosphate dehydrogenase (IMPDH), which catalyzes the first step in de novo biosynthesis of guanosine monophosphate (GMP). Knock-out cassette was constructed with polymerase chain reaction (PCR)-based gene targeting technique for deletion of gua1 gene in S. pombe and this knock-out cassette was transformed to S.s pombe wild type (972h-). Knock-out...

Author(s): Merve YILMAZER, Bedia PALABIYIK, Aysegul TOPAL SARIKAYA and Semian KARAER UZUNER

July 2017

Fourier transform infrared spectroscopy (FTIR) profiling of red pigment produced by Bacillus subtilis PD5

Pigments play an important role in physiological and molecular processes such as photosynthesis, survival to oxidative damage and resistance to ultra violet radiation in microorganisms. This study isolated and characterized pigment produced by Bacillus subtilis PD5 from soil sample of rice field. The pigment was extracted using three solvent system, namely, methanol, n-hexane and ethyl acetate. Fourier transform...

Author(s): Neha Trivedi, Shishir Tandon and Ashutosh Dubey

July 2017

Evaluation of antihyperglycemic, antiradical and acute oral toxicity activities of aqueous leaves extract of Moringa oleifera Lam (Moringaceae) from Benin in normal rats

Moringa oleifera is a commonly cultivated plant in the world. It has a high economic impact owing to its medicinal and nutritional values. Leaves of M. oleifera also contain various constituents that are useful for therapeutic purposes. The aim of this study is (i) to perform phytochemical screening, and to check antiradical and antihyperglycaemic activities of M. oleifera aqueous leaves extract (ii) to control the...

Author(s): LALEYE Obafèmi Arnauld Fernand, AZANDO Erick Virgile Bertrand,, OLOUNLADE Abiodoun Pascal,, TOHOUEGNON Théophile, LALEYE Anatole and AHISSOU Hyacinthe

July 2017

Molecular discrimination of Trypanosoma vivax and Trypanosoma congolense isolates of Nigerian origin

The present study was conducted to assess the molecular discrimination of field isolates of Trypanosoma congolense and Trypanosoma vivax of Nigeria origin in vitro (PCR) whether there will be genetic alteration of the parasites as the infections of the isolates in Yankasa sheep progresses from acute through relapses to chronic stages. A total of thirty Yankasa sheep were acquired from Kastina State in the northern part...

Author(s): C. I. Ogbaje, I. A. Lawal and J. K. P. Kwaga

July 2017

Evaluation of hypolipidemic activity of different extracts of Chomelia asiatica (Linn) in rat fed with atherogenic diet

In the present investigation, the hypolipidemic activity of different extracts of aerial parts of Chomelia asiatica (Linn) is shown. The evaluation dose of different extracts (petroleum ether, ethyl acetate and methanol) of C. asiatica was fixed at 200 mg/kg/day. Hypolipidemic activity was screened by inducing hyperlipidemia with the help of atherogenic diet (AD) in Wistar albino rats and plasma levels of different...

Author(s): Abdul Hameed Thayyil, Arumugam Kottai Muthu and Mohammed Ibrahim

July 2017

Microbial diversity in leaves, trunk and rhizosphere of coconut palms (Cocos nucifera L.) associated with the coconut lethal yellowing phytoplasma in Grand-Lahou, Côte d’Ivoire

The diversity of culturable bacterial and fungal communities was assessed from leaves, trunk and rhizosphere of coconut palms infected and non-infected by the Côte d’Ivoire lethal yellowing (CILY) phytoplasma. Bacterial and fungal microbes were isolated from leaves, trunk and rhizosphere samples collected from two villages of Grand-Lahou, Braffedon and Yaokro, by using a high-throughput...

Author(s): Nadia P. Morales-Lizcano, Ahmed Hasan, Henry S. To, Tacra T. Lekadou, Julia Copeland, Pauline Wang, Hortense A. Diallo, Jean L. Konan Konan, Keiko Yoshioka, Wolfgang Moeder, James Scott and Yaima Arocha Rosete

July 2017

Generating virus-free cassava plants by in vitro propagation with chemical and heat treatment

Cassava production in eastern and Central Africa is severely threatened by the current epidemic of cassava brown streak disease (CBSD). The disease is caused by cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) of the genus Ipomovirus, family Potyviridae. Using virus-free planting material is one effective way to reduce CBSD yield losses in farmer fields. The effects of chemical and heat...

Author(s): Mohammed I. U., Ghosh S. and Maruthi M. N.

June 2017

Role of leucine rich repeat and Ig domain containing 1 (LINGO1) in demyelinating and neurological diseases and investigation of conserved residues by docking analysis for novel therapeutic options

A demyelinating sickness is any ailment of the nervous system in which the myelin casing of neurons is injured. This harm weakens the transmission of signals in the pretentious nerves. Demyelinating diseases, like multiple sclerosis (MS) and Charcot-Marie-Tooth (CMT) disease, are categorized on the basis of the scratch of the myelin covering around neurons, because of swelling and gliosis in the central nervous system...

Author(s): Sumair Kanwal and Shazia Perveen

June 2017

A new immunoregulatory agent from Rosa damascena reduces CD3 expression in human peripheral blood mononuclear cells (PBMCs)

T-Lymphocytes (T-cells) play a dominant role in the initiation and maintenance of autoimmune inflammatory processes. They become activated by the presentation of antigen such as auto antigen. A main feature of T-cell activation is the production of cytokines. Though, uncontrolled T-cells activation through T-cell receptor (TCR) has been linked to numerous autoinflammatory and autoimmune pathologies. This study...

Author(s): Albogami Sarah

June 2017

Evaluation of hygienic quality of ferment of local beer “dolo” used as condiment in Burkina Faso

Rabilé is a ferment of local beer called “dolo” in Burkina Faso. It is used for local beer production and as condiment in sauces. Its process of production could be a source of contamination. So the quality of ferment needs to be guaranteed for consumers’ health. The aim of the study was to control the hygienic quality of local ferment consumed in Burkina Faso. A total of 70 samples were...

Author(s): MOGMENGA Iliassou, SOMDA Kounbèsiounè Marius, KEITA Ibrahim and TRAORE Sababénédyo Alfred

June 2017

Mosquito larvicidal trihydroxylindene derivative from submerged cultures of Trametes species

Larvicidal active pure chemical compound was isolated from a Trametes species using conventional chemistry techniques like solvent-solvent extraction and liquid-solid adsorption techniques. Larvicidal assays carried out on the purified compound, LC50 and LC90 values were calculated and found to be 23.5 and 67.4 ppm, respectively. The chemical structures of the purified compound was elucidated using standard...

Author(s): Abigail Wambui Waweru, Josiah Ouma Omolo, Peter Kiplagat Cheplogoi and Alice Wanjiku Njue

June 2017

Comparison of in vitro antioxidant activity of some selected seaweeds from Algerian West Coast

In vitro antioxidant activity of methanolic (ME) and ethanolic (ET) extracts of six species of seaweeds (Cystoseira stricta, Cystoseira compressa, Corallina elongata, Porphyra umbilicalis, Enteromorpha compressa and Ulva lactuca) collected from Algerian West Coast was evaluated, using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay and reducing power assay. They were tested for total phenolic content. The...

Author(s): Hanane Oucif, Rebiha Adjout, Rahma Sebahi, Farida Ouda Boukortt, Smail Ali-Mehidi and Sidi-Mohamed El-Amine Abi-Ayad

June 2017

Traditional dishes consumed in Zimbabwe and high-performance liquid chromatography (HPLC) quantitation of their antioxidant phytochemicals

Diabetes type 2, cancers, hypertension and obesity have become problematic diseases worldwide calling for more studies on their prevention and management. Antioxidant phytochemicals have shown potential to reduce cases of such chronic diseases therefore the present study quantified antioxidant phytochemicals in traditional dishes consumed in Zimbabwe. Traditional dishes are readily available therefore are cheap and...

Author(s): Dzomba, P., Gwatidzo, L., Mugari, P. and Mupa, M.

June 2017

Interactive effect of colloidal solution of zinc oxide nanoparticles biosynthesized using Ocimum gratissimum and Vernonia amygdalina leaf extracts on the growth of Amaranthus cruentus seeds

Nanofertilizers may be a possible replacement for organic fertilizers because they can get to targeted sites in a plant and either enhance or thwart its growth depending on the toxicity level of the used nanoparticles. Zinc oxide nanoparticles are said to be a good source of nutrients for crop production and using these nanoparticles as bio fertilizers, could help to reduce the chemical residue in the grown vegetables...

Author(s): Rebecca Emmanuel Mfon, Ngozi Ifeoma Odiaka and Andrei Sarua

June 2017

Kinetic characterization and molecular modeling of trehalose-6-phosphate phosphatase from Anopheles gambiae and expressed in Pichia pastoris

Trehalose-6-phosphate phosphatase (TPP) is one of the primary enzymes involved in the synthesis of trehalose, the main sugar found in insect hemolymph. In the present study, we report for the first time heterologous expression in Pichia pastoris, characterization and homology modeling of TPP from Anopheles gambiae mosquito. Purified TPP recombinant exhibited a molecular weight of approximately 36 kDa with optimum pH of...

Author(s): Marcos Cézar Fernandes Pessoa, Érika Izumi, Sandra Patrícia Zanotto and Spartaco Astolfi-Filho

June 2017

Leaf conditioning of Brazilian Cerrado species for DNA microextraction

Proper conditioning of leaf tissues in collection expeditions can affect the quantity and quality of DNA in the extraction process. The aim of this work was to define a method of preserving foliar tissue suitable for obtaining DNA from Brazilian Cerrado trees. Young leaves of species (Mangaba and Baru) were collected and conditioned in five different treatments during a period of six days. Genomic DNA was obtained using...

Author(s): Luiza Gabriela Fulgêncio de Lima, Caio César de Oliveira Pereira, Camila Gracyelle de Carvalho Lemes, Anderson Rodrigo da Silva, Juliana Oliveira da Silva, Dieferson da Costa Estrela, Guilherme Malafaia and Ivandilson Pessoa Pinto de Menezes

June 2017

Effect of Moringa oleifera leaf extract on the haematological and serum biochemistry of rabbits reared in a semi-humid environment

This research is aimed at improving the productive and pathological picture of rabbits reared in a semi-humid environment using Moringa oleifera leaf extract (MOLE). Twenty four mixed- breed rabbits, having an average weight of 700g, were used in this study. They were randomly divided into four equal treatments (6 rabbits each) and gavaged with various concentrations of MOLE. Treatment 1 (control) was given 0ml MOLE/kg...

Author(s): Ojo, O. A. and Adetoyi, S. A.

June 2017

Heavy metal contamination in soils from a municipal landfill, surrounded by banana plantation in the eastern flank of Mount Cameroon

Municipal solid waste generated in our cities, with an unprecedented population growth, has resulted in degrading environmental quality, thus a major problem for policy makers. The extent of Pb, Cu, Zn, Cd, Hg and Zn contamination in soils of the studied area, where vegetables were grown, using metal contamination factor (CF) and pollution load index were assessed. The concentrations of heavy metals studied were in the...

Author(s): Fonge, B. A., Nkoleka, E. N., Asong, F. Z., Ajonina, S. A. and Che, V. B.

June 2017

Improvement of color and increase in anthocyanin content of ‘Niagara Rosada’ grapes with application of abscisic acid

This study aimed to assess the application effect of abscisic acid (S-ABA) on color, and total anthocyanin and phenolic compound contents of the ‘Niagara Rosada’ grapes. The experiment was carried out in a commercial vineyard located in Jales, SP, Brazil, during 2014/2015. The randomized block design was used with four treatments and five replications. The treatments consisted of applying different...

Author(s): Marco Antonio Tecchio, Francisco José Domingues Neto, Adilson Pimentel Junior, Marlon Jocimar Rodrigues da Silva, Sérgio Ruffo Roberto and Ronny Clayton Smarsi

June 2017

DEXseq and Cuffdiff approaches weighing differential spliced genes exons modulation in estrogen receptor β (Erβ) breast cancer cells

While the alternative transcription and splicing mechanisms have long been known for some genes like oncogenes, their prevalence in almost all multi-exon genes has been recently realized with the increasing application of high-throughput experimental methods, named Next Generation RNA Sequencing (NGS). Henceforth, understanding the regulation of these processes in comparisons between cell types and cancer requests,...

Author(s): Noel Dougba Dago, Nafan Diarrassouba, Inza Jesus Fofana, Jean-Luc Aboya Moroh, Hermann-Désiré Lallié, Didier Martial Yao Saraka, Oléfongo Dagnogo, Souleymane Silué, Armel Herve Nwabo Kamdje, Lamine Baba-Moussa and Alessandro Weisz

June 2017

Production potential and chemical composition of elephant grass (Pennisetum purpureum Schum.) at different ages for energy purposes

The elephant grass has gained prominence as one of the main forage species used for biomass production. Therefore, the aim of this study was to identify elephant grass genotypes with high energy biomass production potential by evaluating morpho-agronomic and biomass quality. The following traits were evaluated in this study: dry matter yield (DMY), percentage of whole-plant dry matter (%DM), percentage of neutral...

Author(s): Ana Kesia Faria Vidal, Tatiane da Costa Barbé, Rogério Figueiredo Daher, Janeo Eustáquio Almeida Filho, Roberta Samara Nunes de Lima, Rafael Souza Freitas, Drieli Aparecida Rossi, Érik da Silva Oliveira, Bruna Rafaela da Silva Menezes, Geovana Cremonini Entringer, Wanessa Francesconi Stida Peixoto and Sabrina Cassaro

June 2017

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

Xylooligosaccharides (XOS) are sugar oligomers made up of xylose units with the chain length of 2 to 10 and are considered non-digestible food ingredients. It is mainly produced from xylan hydrolysis. XOS have a characteristic of prebiotic by promoting the growth of probiotic organisms. They have various physiological effects such as reducing cholesterol, maintaining the gastrointestinal health and improving the...

Author(s): Praveen Kumar Gupta, Pushpa Agrawal and Prabha Hegde

June 2017

Immunocapture and simple-direct-tube-reverse transcriptase polymerase chain reaction (RT-PCR) for detection of Rice yellow mottle virus

This study aimed at optimizing the immunocapture (IC) and simple-direct-tube (SDT) -reverse transcriptase polymerase chain reaction (RT-PCR) techniques for detection of Rice yellow mottle virus (RYMV) in order to avoid the extraction of high quality RNA required for molecular methods and avoid costs involved. Rice yellow mottle virus strains and phylotypes were obtained from infected rice leaf samples collected from...

Author(s): Judith Hubert, Herman John Faraji Lyimo, Ashura Luzi-Kihupi and Ernest Rashid Mbega

June 2017

Phytochemical screening and amino acids analysis of mushrooms from Burkina Faso

Mushrooms play an important social, economic and ecological role in the world. In this study, the proximate compositions, minerals and amino acids contents of Russula alveolata, Russula cf. compressa, Russula flavobrunnea var. aurantioflava and Russula ochrocephala from Burkina Faso were investigated. The chemical analysis showed the presence of volatile oil, sterols and triterpenes, carotenoids, saponosides, reducing...

Author(s): Elise Sanon, Jean Claude W. Ouédraogo, Sylvain Ilboudo, Marie K. Laure Guissou, Pierre I. Guissou and Philippe Sankara

June 2017

Establishment, multiplication, rooting and acclimatization of Cabralea canjerana (Vell.) Martius

Seedling production of canjerana (Cabralea canjerana (Vell.) Martius) has been limited by difficulty in germination, caused by recalcitrant behavior of their seeds. The objective of this study was to develop an efficient protocol for conservation and production of canjerana micropropagated plantlets. Seeds of canjerana were disinfected with 0, 2.5, 5.0, 7.5 and 10% of NaOCl solution, at immersion times of 10, 20 and 30...

Author(s): Eliseo Salvatierra Gimenes, Nathalia Pimentel, James Hancock and Dilson Antônio Bisognin

June 2017

Optimised cetyltrimethylammonium bromide (CTAB) DNA extraction method of plant leaf with high polysaccharide and polyphenolic compounds for downstream reliable molecular analyses

Yam (Dioscorea species) and banana (Musa species) leaf sample contain high levels of polysaccharide and polyphenolic compounds. Extraction of good quality DNA from such leaves is usually problematic. Therefore, there is a need to extract good quality DNA in order to perform downstream DNA analysis, especially for the detection of viruses due to their low titration in some infected leaves. The cetyltrimethylammonium...

Author(s): Turaki A. A.,, Ahmad B., Magaji U. F., Abdulrazak U. K., Yusuf B. A. and Hamza A. B.,

June 2017

Agrobacterium-mediated genetic transformation of Fonio (Digitaria exilis (L.) Stapf)

Fonio (Digitaria exilis) is a crop grown in Africa for its excellent culinary and nutritional properties and is a valuable source of income for smallholder farmers. Its improvement has long been neglected by scientists because the crop was one of the lost crops of Africa. Recent advances in Fonio research are centered on its evolution, nutritional and economic values. The lack of a transformation and other biotechnology...

Author(s): Valentine Otang Ntui, Edak Aniedi Uyoh, Ikuo Nakamura and Masahiro Mii

June 2017

Evaluation of sesame (Sesamum indicum L.) germplasm collection of Tamil Nadu for α-linolenic acid, sesamin and sesamol content

A germplasm collection of Tamil Nadu comprising of nine varieties and six landraces were analyzed for their ω3 fatty acid content, lignans such as sesamin and sesamol. The percentage of ALA ranges from 0.1 to 0.55%. A wide variation was observed in sesamol content. The highest sesamol content of 0.547 mg/g was found in a variety SVPR1. The sesamin content ranged between 2.03 and 6.45 mg/g. A negative relationship...

Author(s): Muthulakshmi Chellamuthu, Pavithra Sekar and Selvi Subramanian

June 2017

Morphophysiology of peppermint irrigated with salt water and bovine biofertilizer

Peppermint is a medicinal plant grown worldwide, but it has not been extensively studied, especially the use of saline water for its cultivation. In this sense, the objective of this study is to evaluate the effect of saline waters on peppermint cultivation under the application of bovine biofertilizer. The experiment was carried out from September 2015 to December 2015 in a greenhouse of the Center of Human and...

Author(s): Mario Leno Martins Veras, José Sebastião de Melo Filho, Lunara de Sousa Alves, Kelina Bernardo Silva, Evandro Franklin de Mesquita, Raimundo Andrade, Karialane da Silva Belarmino, Mayara Andrade de Souza, Danrlei Varela Ribeiro, Alvaro Carlos Gonçalves Neto, Uilma Laurentino da Silva, Juliara dos Santos Silva and Ayonna Savana  Fernandes Linhares 

May 2017

Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations

Polymorphism analysis of the Kisspeptin (KISS1) gene and its association with litter size were conducted in two (Gondar and Woyto Guji) Ethiopian indigenous goat populations. Three new pairs of primers were designed for touchdown PCR condition.  A total of 173 and 242 Sanger sequences were employed for further analysis of exon1 (1210 bp) and exon2 (325 bp) regions of the KISS1 gene, respectively. In addition, the...

Author(s): Getinet Mekuriaw,, Joram M. Mwacharo, Tadelle Dessie, Okeyo Mwai, Appolinaire Djikeng, Sarah Osama, Grum Gebreyesus, Alayu Kidane, Solomon Abegaz and Kassahun Tesfaye

May 2017

Establishing sterilization protocol and shoot induction medium for in vitro regeneration of sweetbriar (Rosa rubiginosa L.)

Rosa rubiginosa L. is popular in Lesotho where rural communities harvest the rosehips for sale to the local companies which process them into various products. There is, however, a challenge in establishing plantations of the species due to deep seed dormancy and so difficulty in germination. In this study, nodal explants of R. rubiginosa L. were sterilized with 20 g·L-1 Ridomil Gold MZ (RGM) and cultured on...

Author(s): Miloane Meshack Mokhobo, Remmy Wekesa Kasili and Amos Emitati Alakonya

May 2017

Biodiesel production from marine microalgae Nannochloropsis gaditana by in situ transesterification process

Microalgae is one of the best sources of renewable energy production, such as biofuels. The production of biodiesel from microalgae has several advantages, including the high productivity of lipid and the possibility of cultivating them on marginal land. One of the challanges in using microalgae for biodiesel production is the complexities process of lipids extraction by organic solvents followed by transesterification....

Author(s): BENZIDANE Dehiba, BABA HAMED Mohamed Bey and ABI-AYAD Sidi-Mohammed El-Amine

May 2017

Truffle mediated (Terfezia claveryi) synthesis of silver nanoparticles and its potential cytotoxicity in human breast cancer cells (MCF-7)

Terfezia claveryi is a species that belongs to the genera of Terfeziaceae or desert truffles, which is a family of truffles. In the present study, silver nanoparticles were synthesized from aqueous extract of T. claveryi which are in the range of 25 to 60 nm. The synthesized nanoparticles were characterized by ultraviolet-visible (UV-Vis) spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray diffraction...

Author(s): Habeeb Khadri, Yousef H. Aldebasi and Khateef Riazunnisa

May 2017

Genetic and morphological diversity among sweet potato (Ipomoea batatas (L) Lam.) accessions from different geographical areas in Malawi

An understanding of morphological and genetic diversity of sweet potato landraces is fundamental to any breeding program in a country. Fifty-nine sweet potato accessions from three eco-geographical populations of northern, south eastern and southern Malawi were examined using ten Simple Sequence Repeats (SSR) loci and seven International Board for Plant Genetic Resources (IBPGR) descriptors of sweet potato. The study...

Author(s): Felistus Chipungu, Wisdom Changadeya, Aggrey Ambali, John Saka, Nzola Mahungu and Jonathan Mkumbira

May 2017

Postpartum serum biochemical profile of Sudanese cystic ovarian crossbred dairy cattle

Cystic ovarian disease (COD) is an ovarian dysfunction in cows resulting in a serious economic loss in the dairy industry. This study was conducted to examine the hemoglobin (Hb) concentration, serum total protein (TP), phosphorus (P), copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) levels of Sudanese crossbred (Friesian x Kenana) cows with COD in semi-closed condition. Forty-five dairy cows were divided into two...

Author(s): Nasser Mohammed Osman, Imadeldin Elfaki, Faisal Omer Ahmed and Abdelrahim Hommeida

May 2017

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

Pseudomonas aeruginosa is intrinsically resistant to several antimicrobial drugs classes. Various extended-spectrum beta lactamases (ESBL) types have been found in P. aeruginosa such as Pseudomonas extended resistance (PER) and Guiana extended-spectrum (GES) enzymes. The study aimed to evaluate the susceptibility of the ESBL producing P. aeruginosa strains that express blaGES and blaPER genes to commonly used...

Author(s): Omar B. Ahmed and Atif H. Asghar

May 2017

Genetic diversity and population structure of Ethiopian finger millet (Eleusine coracana (L.) Gaertn) genotypes using inter simple sequence repeat (ISSR) markers

Eleusine coracana is an annual allotetraploid (2n=4x=36) that belongs to grass family, Poaceae. This research aimed to investigate level of genetic diversity of 80 accessions using ISSR markers. DNA was extracted from a bulk of three plants per accession using a modified CTAB method. Six ISSR primers amplified a total of 45 clear and reproducible bands. The total genetic diversity (H) and Shannon’s diversity...

Author(s): Haftom Brhane, Teklehaimanot Haileselassie and Kassahun Tesfaye

May 2017

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

A Gram-positive, rod-shaped, spore-forming haloalkaliphilic bacterium designated as NA7 was isolated from the surface of a Helianthemum nummularium root sample obtained from Wadi Natrun in Egypt. Sequence analysis of the 16S rRNA gene revealed a Bacillus haloalkaliphilius strain as the closest match with 99% identity. In a shake flask culture containing 10% NaCl, adjusted to pH 10 and incubated at 37°C, the isolated...

Author(s): Walid Ahmed Lotfy, Neveen Mohamed Abd-El-Karim, Ebaa Ebrahim El-Sharouny and Ehab Ragheb El-Helow

May 2017

Diversity of Aspergillus isolates and selection of an isolate with high β-fructofuranosidase activity that is native to the Peruvian coast

In order to obtain a native isolate with high β-fructofuranosidase activity (FFase), a preliminary selection of 44 isolates, predominantly formed by Aspergillus niger (72%), was performed among  167 isolates of Aspergillus native to the Peruvian coast (Casa grande, Cartavio, Paramonga and Tacama). In addition, genetic diversity was studied using molecular markers, Inter Simple Sequence Repeats (ISSR). The...

Author(s): Boris Gutarra Castillo, Marina Díez-Municio, Susan Canchaya Garcia and Gaby Calixto Zacarias, Juan Cisneros de la Cruz and F Javier Moreno

May 2017

Myracrondruon urundeuva Allemão: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and...

Author(s): André Luiz Lima Menezes Santos, José Davi Prado Lima, Clivia Rolemberg Andrade, Deisylaine Maria Santos, Antonio Santos Dias, Pietra Alexia Lima Santos, Rafaela Karolina Vianna Nunes, José Evaldo Rodrigues Menezes Filho, José Nilson Andrade Santos, Carla Maria Lins Vasconcellos, Andrea Yu Kwan Shan, Brancilene Santos Araujo and Charles Santos Estevam

May 2017

Chromosomal and plasmid mediated degradation of crude oil by Bacillus coagulans, Citrobacter koseri and Serratia ficaria isolated from the soil

In this work, the degradative abilities of some bacteria isolated from the soil were studied. Five distinct isolates were subjected to substrate susceptibility test to determine their potential degradative abilities in the presence of different hydrocarbons. The best three crude oil degraders were selected and their identities were further established using API technique. Furthermore, the extent of each degradative...

Author(s): Fagbemi O. Kehinde and Sanusi A. Isaac

May 2017

Effect of different concentrations of bush passion fruit pulp and temperature in the production of beer

Among many species of passion fruit, the bush passion fruit (Passiflora cincinnata Mast.) is a wild, edible fruit, of exotic flavor and has good acceptability for consumption. This study aimed to determine the physico-chemical characteristics of the bush passion fruit pulp, and to produce and characterize physico- chemically the ale beer made with bush passion fruit as a malt adjunct, using different percentages (10,...

Author(s): Verena Fiscina de Melo, Geiza Suzart Araújo, José Ailton Conceição Bispo, Vanildo Luiz Del Bianchi and Giovani Brandão Mafra de Carvalho

May 2017

Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province

The objective of this research was to obtain quantitative growth hormone gene polymorphism association between growth hormone gene genotype with quantitative characteristics of the thin-tail sheep in the highlands and lowlands of Jambi Province. Two phases of research were done on the field and in the laboratory. Field research which was conducted to obtain the quantitative characteristics data includes; withers height...

Author(s): Depison, Sarbaini Anwar, Jamsari, Arnim and Yurnalis

May 2017

Exploration of Sri Lankan soil fungi for biocontrol properties

Sri Lankan soil is a rich source of fungi with new strains that are not well explored to date. In the present study, a total of 83 soil fungi were isolated from different locations of Sri Lanka using chitin and β-1,3-glucan selective media. Of the 83 isolates, 22 isolates showed high biocontrol activities against seven selected plant pathogenic fungi in dual culture plate assay. These 22 isolates were further...

Author(s): Herath H. H. M. A. U., Wijesundera R. L. C., Chandrasekharan N. V. and Wijesundera W. S. S.

May 2017

Computer simulation of benzene, toluene and p-xylene adsorption onto activated carbon

In this work, the adsorption of benzene, toluene and p-xylene on activated carbon was verified through a computer simulation. A carbon model was used as the work basis. Several steps were completed from the modeling of the carbon structures and pollutants to simulations of molecular dynamics. After analyzing the results, it was found that the groups: ether, lactone and carbonyl (ketone) present in the carbon structure...

Author(s): Wanessa Almeida da Costa, Carlyle Ribeiro Lima, Frederico Guilherme Santana da Silva Filho, Mozaniel Santana de Oliveira, Renato Macedo Cordeiro, Raul Nunes de Carvalho Junior, Marlice Cruz Martelli and Davi do Socorro Barros Brasil

May 2017

The influence of potassium dichromate on growth and survival of the vermicomposting earthworm Eisenia foetida

The present research evaluated the redox ability of Cr6+ in the earthworm Eisenia foetida, and its effects on growth and survival. An assay was conducted with an increasing doses of K2Cr2O7 (0, 200, 400, 800, 1000, and 1200 mg K2Cr2O7 kg-1 substrate) applied to a vermicomposting system which was inoculated with 30 earthworms per chamber. Earthworm growth, estimated by weight, showed a quadratic response (p = 0.03) with...

Author(s): Martha Angélica Ramírez Tabche, Juan José Peña Cabriales, María Maldonado Vega and José Antonio Vera-Núñez

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