Effect of sample extraction, preparation methods on HPLC quantification of plumbagin in in vivo and in vitro plant parts of Plumbago zeylanica L.
August 2018
Plumbagin is an important therapeutic compound of Plumbago zeylanica L., the commercial demand of this compound warrants optimizing a suitable method to isolate plumbagin in large scale. The present study was undertaken to obtain the maximum recovery of plumbagin content by employing different extraction methods viz., ultra-assisted extraction (UAE), maceration extraction (ME), soxhlet extraction (SE), serial soxhlet...
Biomanagement of Rose and carnation wastes in flower industries with three epigeic earthworm species: Eisenia fetida, Eisenia andrie and Dendrobanae veneta
August 2018
The horticulture sector in Ethiopia is being challenged by multifaceted hitches, among which managing excess wastes produced from the farms and minimizing the cost of inorganic fertilizers are the major ones. The performance of three epigeic earthworm species, Eisenia fetida, Eisenia andrie and Dendrobanae veneta, in managing flower (Rose and Carnation) wastes through vermicomposting was evaluated. The study was done...
In vitro regeneration of Neocarya macrophylla (Sabine) Prance, wild fruit of Niger
August 2018
Neocarya macrophylla is a spontaneous fruit species that constitutes the dominant population of Dallol Bosso in Southwestern Niger. In order to test the in vitro regeneration ability of this species, micro-propagation was applied to micro-cuttings from young almond seedlings. The Murashige and Skoog basal medium was enriched with several concentrations of naphthaleneacetic...
Comparative analysis of the chemical compositions of indigenous watermelon (Citrullus lanatus) seeds from two districts in Limpopo Province, South Africa
August 2018
Seeds from different indigenous watermelons were analysed for their chemical composition such as phenolics, proteins, oils, minerals, ash and fibre content as well as antioxidant activity measured in methanol extract using 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. The morphological variations of watermelons, which revealed diversity in terms of fruit and seed colour were also assessed. Average percentages for lipid...
Circular RNA expression profiles of peripheral blood mononuclear cells in rheumatoid arthritis patients
August 2018
Rheumatoid arthritis (RA) is a systemic, chronic autoimmune disease involving synovial inflammation and joint destruction. Lack of knowledge on RA pathogenesis hinders development of effective treatments. The aim of this study was to examine whether circular RNAs (circRNAs), which are involved in the development of other diseases, may also play a role in RA. Expression profiles of circRNAs were compared between...
Evaluation and comparison of staining effect of Punica granatum flower extract on testis and ovary of Wistar rats: First results
August 2018
The evaluation and comparison of the staining effect of pomegranate (Punica granatum) flower extract (dye solution) on histological sections of rat testis and ovary provides a simple, quick technique for the visualisation of both cells. In the developed procedure, this work shows the staining effect of P. granatum flower extract used at different pH of dye bath and temperatures on the histological sections of rat testis...
Nuclear isolation and purification using SDS/urea (NIPSU) method for efficient and rapid extraction of high-purity genomic DNAs from Jatropha curcas L: A comparative analysis of DNA isolation protocols
August 2018
Jatropha curcas L. (Jatropha) has gained popularity for its potential use in biodiesel production in arid regions, and its yield improvement by molecular breeding has been anticipated. However, Jatropha is known for its recalcitrance in the extraction of pure genomic DNAs. In this study, four DNA extraction methods were comparatively examined for their efficiency and quality of genomic DNA isolations from Jatropha....
Genetic variability, heritability and expected genetic advance of yield and yield related traits in common bean genotypes (Phaseolus vulgaris L.) at Abaya and Yabello, Southern Ethiopia
August 2018
This experiment was conducted to evaluate 36 common bean genotypes including seven released varieties to generate information on the extent of genetic variability, heritability and expected genetic advance of yield and yield related traits. The field experiment was conducted in 2015 at two locations (Abaya and Yabello) and genotypes were planted in triple lattice design. Data were collected on yield and important...
Technological properties and sugar tolerance performance of palm wine yeasts isolated from parts of Nsukka, Nigeria
August 2018
The ability of yeasts to survive and produce significant ethanol in high sugar medium and high ethanol environment is essential for the use of such yeasts in industrial and edible ethanol production. Four Saccharomyces spp. strains () isolated from palm wine and an industrial strain were studied in high glucose medium for ethanol production and ability to survive in high sugar medium. Saccharomyces cerevisiae...
In vitro inhibitory potentials of aqueous and ethanol extracts of Hyptis suaveolens on fungi associated with postharvest spoilage of Brassica oleracea
August 2018
The inhibitory potential of Hyptis suaveolens leaf and root extracts on fungi associated with postharvest spoilage of cabbage (Brassica oleracea var. capitata) was examined. Collection of diseased cabbage was done in sterile sample bags . Isolation of fungi was carried out by inoculating small sections of diseased tissues excised from the cabbages unto Petri-dishes containing Acidified Potato Dextrose Agar (APDA)....
Application of molecular and biotechnological techniques in plant disease management: A review
August 2018
Plant diseases are a major challenge in crop production. They are caused by nematodes, bacteria, fungi, viruses as well as plant nutritional factors. Diseases interfere with the normal physiological and metabolic processes of plants. This results in various effects including wilting, stunting, yellowing and death of plant tissues and organs. Crop losses due to diseases manifest in form of reduced yield, poor quality...
Biosurfactant production by Bacillus subtilis UFPEDA 86 using papaya (Carica papaya L.) waste as substrate: Viability studies and pH influence of the culture medium
July 2018
Biosurfactants are surface-active compounds derived from microorganisms and offer several advantages over chemical surfactants, such as low toxicity, good biodegradability and ecological acceptability. Even though interest in biosurfactants is increasing, these bioproducts do not compete economically with synthetic surfactants due to the overall costs of the bioprocess. The use of inexpensive raw materials is an...
Evaluation of Curcuma zerumbet (Zingibaraceae) rhizome extracts sub-acute toxicity on Wistar rats
July 2018
Plant extracts have been lately used by the population to treat various types of diseases, and this has been notably encouraged by the World Health Organization (WHO). Curcuma zerumbet (Zingiberaceae) belonging to the family of the Zingiberaceae, is herbaceous, perennial and, utilized by the population to treat gastric disorders. However, data on the subacute toxicity of this species are scarce in the literature....
Biotransformation of the residual liquid from the wet coffee benefit by Kluyveromyces marxianus
July 2018
The search for biotechnological alternatives for the use of residuals generated in the agro-industrial processing of coffee is a current problem. This study evaluated the biotransformation of the liquid residual of the humid coffee benefit using the yeast Kluyveromyces marxianus CCEBI 2011. It was demonstrated that this strain is able to effectively use the reducing and neutral sugars in 24 h, for a yield of 40% in the...
Assessment of morphological characteristics among upland rice (Oryza sativa and Oryza glaberrima) germplasm
July 2018
Rice is an important staple food crop that feeds over half of the global population and has become the cereal that provides a major source of calories for the urban and rural poor in Africa. This work aimed to evaluate the morphological of rice (Oryza sativa and Oryza glaberrima) germplasm. In the present study, 14 quantitative traits were used across 48 accessions or genotypes obtained from Central Agricultural...
Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis
July 2018
A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK-043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination...
Effect of seasonal variations on the yield of essential oil and antioxidant of Achillea fragrantissima (Forssk) Sch. Bip
July 2018
Temperature stress is becoming the major concern for plant scientists worldwide due to climate change. Temperature stress has devastating effects on plant growth and metabolism. The aim of the study was to investigate the effect of climatic seasonal change on the yield and composition of essential oil of the plant, Achillea fragrantissima. The gas chromatography-mass spectrometry (GC-MS) used to analyze the essential...
Effects of aqueous seed extracts of Mucuna sloanei (Fabaceae) on body weight and some biochemical parameters of Rattus novergicus
July 2018
Mucuna sloanei is an annual leguminous plant widely used among the various ethnic groups in Nigeria. The effects of aqueous M. sloanei seed extract on the body weight and some biochemical parameters of 48 normal male Rattus novergicus (albino rats) were investigated for 28 days. The rats were divided into control group (A) which received distilled water and treatment groups (B, C and D) that received oral administration...
Potential evaluation of Saccharomyces cerevisiae strains from alcoholic fermentation of mango pulp
July 2018
The fermentation characteristics of different strains of Saccharomyces cerevisiae isolated from sugar cane musts for the production of cachaça (Brazilian sugar cane spirit) and from beer musts were analyzed based on the kinetic parameters of alcoholic fermentation in mango pulp. The commercial pressed baker’s yeast was used as a standard inoculum. The results show that the yeast strains tested from sugar...
Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens
July 2018
Antibacterial activity of extracts of Zanthoxylum gilletii, Markhamia lutea and their fungal endophytes were evaluated against bacterial pathogens of common bean: Xanthomonus axonopodis pv. phaseoli and Pseudomonus syringae pv. phaseolicola. The leaves of both plants were dried under shade, ground to fine powder and extracted using methanol. The methanol extracts were fractionated sequentially using ethylacetate and...
Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)
July 2018
The aim of this study was to investigate the transmission of the lethal yellowing disease (LYD) of coconut tree caused by a phytoplasma from the zygotic embryo to the regenerated plantlet in vitro. From a total of 30 trees, 150 mature coconut nuts where harvested. These nuts were used to extract 150 zygotic embryos. From this package, 96 zygotic embryos were used to regenerate 96 young coconut seedlings in vitro and the...
Comparative evaluation of the physicochemical and pasting properties of flour from three varieties of Brachystegia spp.
July 2018
The chemical compositions, functional and pasting properties of flour from three varieties of Brachystegia spp. (Brachystegia eurycoma, Brachystegia nigerica and Brachystegia kennedy) were studied. Results show that the chemical compositions of flour samples ranged from12.31 to 12.67% (protein), 1.66 to 1.72% (crude fiber), 2.06 to 2.39% (ash), 7.18 to 8.45% (fat), 3.85 to 4.75% (sugar) and 58.45 to 59.62% (starch)....
Enhancement of somaclonal variations and genetic diversity using graphite nanoparticles (GtNPs) in sweet potato plants
July 2018
To assay the efficiency of graphite nanoparticles (GtNPs) in sterilizing tissues and their role in enhancing genetic diversity, sweet potato is considered an important crop; hence its explants were used. In this experiment, GtNPs of 200, 400 and 800 ppm concentrations were used for sterilization of MS callus induction and regeneration media in Abees cultivar. The results showed that GtNPs had a good potential for...
Phytochemical profile and biological activities of Momordica charantia L. (Cucurbitaceae): A review
July 2018
This study discusses the bioactive composition, supercritical fluid extraction and biological activities of Momordica charantia L. from the last five years. Numerous compounds that have been identified in the extracts of M. charantia, including phytosterols, terpenoids, fatty acids, phenolic compounds, phenolic acids and flavonoids were also discussed. Although, several studies reported the use of organic solvents in...
Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database
June 2018
Common bean (Phaseolus vulgaris) is a rich source of protein, vitamins, and micronutrients and is an important crop for food security throughout Latin America, Asia, and Africa. Among tropical and subtropical regions of the world, where the majority of beans are grown, yield losses due to the pathogenic fungus Pseudocercospora griseola, causing angular leaf spot (ALS), can be as high as 80%. The strategic use of...
Diversity and genetic identity of pineapple [Ananas comosus (L.) Merr.] in Tanzania based on microsatellite markers
June 2018
Pineapple [Ananas comosus (L.) Merr.] is an important fruit crop cultivated in Tanzania. However, the knowledge on genetic diversity of the pineapple cultivars grown in Tanzania is limited. This study was aimed at determining the genetic diversity and identity of pineapple cultivars from different growing regions in Tanzania using microsatellite markers also known as simple sequence repeat marker (SSR). Ten of the 18...
Bio-prospecting of macro-algae for potential industrial dyes
June 2018
Synthetic dyes have been used for different purposes in various fields, but their continued usage has presented both environmental and health challenges. Therefore, alternative safer dye sources are inevitable. Algae have different pigments with potential for exploitation and usage in different socio-economic sectors. The aim of this study was to bio prospect dyes from macro-algal species of the Indian Ocean marine...
Activation of TLRs/NF-κB signaling pathway and production of diverse cytokines in the incidence and development of invasive pulmonary aspergillosis
June 2018
Aspergillus fumigatus is a common saprophytic fungus in the air. It can cause a severe infectious disease called invasive pulmonary aspergillosis (IPA). To establish the activation of TLRs/NF-κB signaling pathway in invasive pulmonary aspergillosis (IPA) model of wild type mouse, three groups of mouse were used in the study; the normal mice; mice infected with A. fumigatus conidia; IPA mouse infected with A....
Cost effective medium for in vitro propagation of Tanzanian cassava landraces
June 2018
Cassava (Manihot esculenta Crantz) is a staple food for over 800 million people in the tropics. However, its production is constrained by an inadequate supply of clean planting materials. Tissue culture carried out in laboratories is one established method for the mass production of clean planting materials. However, the cost of conventional tissue culture is high and the cassava industry would benefit from an...
Studies on styrene concentration in drinking water and hot beverages in some settings
June 2018
Water bottles and cups composed of polystyrene also contain non-polymerized styrene. Styrene’s toxicological profile is associated with several health issues for humans. Mainly, the central and peripheral nervous systems are highly disturbed by styrene ingestion. Styrene is also considered to be a carcinogenic agent and has been linked to cancer. The HPLC method was validated through prepared QC samples. The HPLC...
Microsatellites markers associated with resistance to flower bud thrips in a cowpea F2 population derived from genotypes TVU-123 and WC36
June 2018
Breeding for resistance to flower bud thrips (Megalurothrips sjostedti) in cowpea has been hindered by the quantitative nature of resistance. To identify simple sequence repeat (SSR) markers associated with resistance to flower bud thrips that could be used for marker-assisted breeding, a F2 population was generated from a cross between genotypes TVU-123 (resistant) and WC36 (susceptible). The population was evaluated...
Studies on enhanced African black soap from Theobroma cacao (cocoa) and Elaeis guineensis (palm kernel oil)
June 2018
The indigenous African organic soap is formed by saponification. Using the local and ancient method, with slight modifications, palm kernel oil (Elaeis guineensis) and the filtrate of burnt cocoa pod ash (Theobroma cacao) were used to prepare African black soap (C11H23COO-K+). The prepared black soap was thereafter divided into five parts. Part A was the control without additives, while parts B, C and D were enhanced...
Identification and genetic diversity of Jordanian potato soft rot isolates, Pectobacterium carotovorum subspecies carotovorum (DYE 1969)
June 2018
Bacterial soft rot disease is one of the most important factors affecting potato production in Jordan. Based on biochemical and physiological analyses, Jordanian isolates of soft rot from potato were identified as Pectobacterium carotovorum subspecies carotovorum (DYE 1969). Phylogenetic analysis using pmrA gene sequence data showed that isolates from different regions were closely related to each other, whereas little...
Surface plasmon resonance and antimicrobial properties of novel silver nanoparticles prepared from some indigenous plants in Uyo, Nigeria
June 2018
Synthesis of nanoparticles was done by green method. Aqueous extracts of Vernonia amygdalina, Telferia occidentalis and Lasianthera africana were used as the model vegetables. Silver nitrate was used as the silver precursor, while the plant extracts served as the reducing agents and Xanthan gum (0.25, 0.5, 0.75 and 1.00% w/v) was introduced as stabilizing agents. Twelve batches of silver nanoparticles were synthesized:...
Morphological variability and molecular characterisation of thirty soybean genotypes using random amplified polymorphic DNA (RAPD) markers
June 2018
The genetic and morphological diversity of soybean germplasms was studied using random amplified polymorphic DNA (RAPD) markers and morphological traits such as plant height, height of cotyledon leaves, basal leaf length, basal leaf width, basal leaf area, terminal leaflets number, terminal leaflet length, terminal leaflet width, terminal leaflet area and basal leaf petiole length. A total of 30 germplasms were...
Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia
June 2018
Chickpea (Cicer arietinum L.) is an important leguminous crop grown in different parts of Ethiopia. It is a nutritionally valued and N2-fixing legume which forms a symbiotic association with Mesorhizobium. This study was conducted to characterize and evaluate symbiotic efficacy of chickpea rhizobia isolated from soil samples collected from the study area. Seventeen chickpea rhizobia were isolated by soil host plant trap...
Production of single cell protein (SCP) and essentials amino acids from Candida utilis FMJ12 by solid state fermentation using mango waste supplemented with nitrogen sources
June 2018
In Burkina Faso, deficiency of amino acids in protein is becoming a major healthy public problem. This study was purposed to optimize essentials amino acids in single cell protein (SCP) by supplementing different nitrogen sources during fermentation of mango waste with Candida utilis FJM12. Analytical methods were used to determine biomass yield, chemical composition and amino acids profile of SCP. The principal...
Development and validation of analytical methodology for quantification of total flavonoids of Morus nigra by ultraviolet-visible absorption spectrophotometry
June 2018
Morus nigra L. is known in the region of Brazilian São Francisco Valley as "amora-miúra". It is widely used in traditional medicine, mainly in treatment of diabetes, hypercholesterolemia, cardiovascular problems, obesity and gout. This study proposed to develop and validate a fast, simple, efficient and low cost analytical method that could quantify total flavonoid content present in crude...
Isolation and screening of antibiotic producing actinomycetes from rhizosphere and agricultural soils
May 2018
Antibiotics are secondary metabolites produced by microorganisms. Actinomycetes are the top producers of antibiotics. The study was designed to isolate and screen antibiotic producing actinomycetes obtained from rhizosphere of plants and agricultural soils. A total of 30 actinomycetes were isolated. Out of the 30 actinomycetes, 18 (60%) showed antimicrobial activities against one of the tested microorganisms of which 9...
Screening for total antioxidant activity, flavonoids and phenolics variability in forty-five accessions of roselle (Hibiscus sabdariffa L.)
May 2018
Build-up of free radicals in the human body can cause oxidative stress which may invariably lead to degenerative diseases and eventual death. Antioxidants such as polyphenols and flavonoids found in fruits and vegetables have been shown to protect the body against the damaging effects of these free radicals. Roselle contains high amounts of antioxidants and its leaves are often used for sauces or in herbal preparations...
Interrelationships of quantitative traits and genetic variability of Desi type chickpea genotypes as revealed by agro-morphology and inter simple sequence repeat markers
May 2018
Plant breeders need to stay alert in developing new cultivars on a steady basis to respond to the need of ever increasing human population by exploring either natural variability present in germplasms or generating new variability. Field experiment was conducted at five locations of Western Ethiopia during the 2016/2017 main cropping season to study interrelationships and genetic variability present in Desi type...
Molecular evidence of Helicobacter suis infection in pigs in Nigeria
May 2018
Helicobacter suis mainly colonizes the stomach of pigs and occasionally infects humans. It is a prevalent cause of gastritis and gastric ulcers in both species. This study was designed to determine the colonization rate of H. suis in the stomach of pigs in Nigeria. Pig slaughter house surveys were conducted and stomach mucosa samples were collected from the fundus of the stomach of a total of 160 pigs in four locations...
Marine killer yeast Metschnikowia saccharicola active against pathogenic yeast in crab and an optimization of the toxin production
May 2018
A marine yeast strain, DD21-2 was isolated from sediments in Dandong, China, which has killer activity against yeast Metschnikowia bicuspidata WCY (pathogenic to crab, Portunus trituberculatus). Routine identification, sequence analysis of 26S rDNA and ITS sequencing showed that the strain was M. saccharicola DD21-2, and has not been previously reported as having killer activity against M. bicuspidata WCY. To optimize...
Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis
May 2018
In the present study, an efficient protocol for somatic embryogenesis and plant regeneration was established in six finger millet varieties (GBK-043137, GBK-043128, GBK-043124, GBK-043122, GBK-043094 and GBK-043050). Shoot tips from 3 days in vitro grown plants were inoculated on MS supplemented with various concentrations and combinations of α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D),...
Simple sequence repeat (SSR) markers linked to drought tolerant traits in selected Sudanese rice (Oryza sativa L.) genotypes
May 2018
Rice growth and productivity are affected by biotic and abiotic stresses; key among them being drought. Conventional breeding for drought tolerance is slowed down by the complex nature of mechanisms underlying this stress although molecular markers offer a promising approach. Plant exposure to drought stress leads to a significant effect in chlorophyll content and reduction in photosynthesis. In this study, 23 Sudanese...
Light quality and plant growth regulators influence pigment production in Alternanthera brasiliana calli
May 2018
Alternanthera brasiliana is a medicinal plant with several popular use and high production of betalains. The aim of the present research was to establish a protocol for callus induction and betalain biosynthesis in A. brasiliana species, as well as to investigate the effect of different qualities of light in increasing betalain in A. brasiliana callus cultures. In this way, the callus initiation and the improvement of...
Presence of Bacillus thuringiensis (Bt) gene in cereals and cereal-based products in local markets and supermarkets of Yaoundé, Cameroon
May 2018
The use and importation of genetically modified (GM) crops and products derived from these crops are regulated by national and international policies, which unfortunately are often not properly implemented in some countries. Given the ongoing globalization of trade and increasing availability of GM plant products, countries like Cameroon with a weak system to regulate the importation of these products face the threat of...
Effects of ethanolic extract of Waltheria indica aerial parts on some liver and kidney function indices in albino rats
May 2018
This study evaluated the toxicological effects of chronic treatment with ethanolic extract of Waltheria indica aerial portion on body weight, hematological and biochemical parameters in albino rats using standard methods. Rats treated with 400 mg/kg body weight (bw)/day of the extract showed no behavioural changes. However, there was general reduction of activity in rats given 800 and 1,600 mg/kg bw/day of the extract....
Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone
May 2018
A study on rapid propagation of cassava through tissue culture was conducted with three elite cassava genotypes: Slicass 6, Slicass 11 and Cocoa from Sierra-Leone. They showed slow growth in Murashige & Skoog (MS) basal medium which was proven to be optimal for a vast number of cassava accessions. Prior to mutation induction, a large population needs to be produced for mutagen susceptibility test and for mutant...
Azospirillum spp. potential for maize growth and yield
May 2018
The importance of biotechnology involved in the availability of nutrients to plants in different production systems is well known. In the search for agricultural sustainability, biological nitrogen fixation process stands out, especially in tropical regions where soil organic matter can be rapidly mineralized. In this aspect, researches have pointed out the potentialities of the use of diazotrophic bacteria, as well as...
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