African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12172

AJB Articles

April 2017

Histochemical GUS expression of beta tubulin promoter in transgenic tobacco

Agrobacterium mediated plant transformation is a useful technique for stable transformation of plants. This study aimed at stable transformation of the beta tubulin (β) gene promoter and its expression analysis by histochemical GUS assay. The beta tubulin promoter sequence was cloned in plant expression vector (pGA482) and transformed stably in tobacco through Agrobacterium mediated transformation. The stable GUS...

Author(s): Hira Mubeen, Aftab Bashir, Ayesha Ameen, Ammara Masood and Shahid Raza

April 2017

Phenotypic diversity in physic nut (Jatropha curcas L.) in vivo germplasm bank for superior parent selection

Jatropha curcas is an interesting alternative for biodiesel production due to the high oil content in its seeds, its ability to grow in a wide range of climate and soil conditions as well as low cost of production. However, the species is considered to be in domestication and there are no defined cultivars. Therefore, it is extremely important to understand the genetic diversity of the species for selection and...

Author(s): Amanda Carolina Prado de Moraes, Fabiana Mota da Silva, Aretha Arcênio Pimentel Corrêa, Antônio Orlando Di-Mauro and Sandra Helena Unêda-Trevisoli 

April 2017

Chemical composition and antioxidant activity of Lippia alba essential oil obtained by supercritical CO2 and hydrodistillation

Lippia alba essential oils were obtained by hydrodistillation (HD) and supercritical fluid (SFE) extraction methods. These were analyzed by gas chromatography-mass spectrometry-flame ionization detector (GC-FID-MS). Antioxidant activity was tested by DPPH and ABTS methods, and total soluble phenolics (TSP) were also determined. While in the SFE extract 14 compounds were identified (mostly: myrcenone 3.4%,...

Author(s): L. Reyes-Solano, A. P. Breksa III, J. Benigno Valdez-Torres, M. Angulo-Escalante and J. B. Heredia

April 2017

Modelling the rheological properties of gruels produced from selected food products from Cameroon

This study investigated the flow behaviour of some food gruels obtained from pre-treated banana, sorghum or sesame flours. The Herschel-Bulkley and the power law models were used to evaluate the consistency and flow indices while an Arrhenius-type equation was used to analyse the effect of temperature on viscosity. The Ostwald de Waele model gave a good fit with experimental data, with p-values less than 0.01 and R2...

Author(s): Cybèle Maka Taga and Yvette Jiokap Nono

April 2017

Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis

The objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were...

Author(s): Rokiatou Fané, Adounigna Kassogué, Diakaridia Traoré, Sognan Dao. Kadia Maïga, Aliou Bah, Fernando Hercos Valicente and Amadou Hamadoun Babana

April 2017

Computational antioxidant capacity simulation assay of Garcinia kola (Heckel) seed extracts

Polypharmacological activities of the biflavonoid fraction of Garcinia kola seed justify its development as a nutraceutical, however, quality assurance of active nutraceutical ingredient (ANI) requires conformance to appropriate standards of composition and quality. It was hypothesized that variation in extraction protocols, as previously reported for the biflavonoid fraction, would lead to variation in extract...

Author(s): Sunday Olakunle Idowu, Morenikeji Ayodele Adeyemo, Segun Abidemi Aderibigbe and Oluwabukola Idowu Omotayo

April 2017

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

Antimicrobial resistance developed in several pathogens poses an increasing threat to human health across the world. No country can escape from the medical and economic impacts from this serious problem. Although the antibiotic resistance is not a new phenomenon, the current magnitude and speed with which it is developing is a cause for the global concern including in India. There are so many common diseases resulting...

Author(s): Nirbhavane H. M. and Bagde U. S.

April 2017

Colostrum immune quality of local sow breed in Benin: Growth, survival and acquisition of passive immunity in new-born piglet

The objective of this study was to evaluate husbandry practices of pregnant sows, immunoglobulin G (IgG) concentration in colostrum of sow from local breed of Benin, their transfer to litter and the survival and growth of piglets until 42 days of age within traditional farms pig. Results showed that sows were raised according to traditional practices characterized by sustainable or precarious housing without any hygiene...

Author(s): Aristide Mahoutin Agbokounou, Gbênangnon Serge Ahounou, Zakaria Bengaly, Issaka Youssao Abdou Karim, Guy Apollinaire Mensah, Bénoît Koutinhouin and Jean-Luc Hornick

April 2017

Effect of growth regulators on indirect organogenesis of two grapevines (Vitis vinifera L.) cultivars

Establishment of efficient protocol for high-frequency of indirect regeneration of plantlets has a vital role in the analysis of genetic material in mass propagated plants. The optimal levels of growth regulators and light conditions were investigated on callus induction and organogenesis of cultured grapevine from in vitro tissues. Maximum calluses and shoots were produced by using medium supplemented with four...

Author(s): Fikadu Kumsa

April 2017

Comparative effects of plant growth promoters and earthworms (Millsonia anomala) on rooting of cocoa orthotropic cuttings

Propagation of cocoa trees with high yield and disease resistance is envisioned for the renewal of the cocoa orchard. The dissemination of these elite varieties by orthotropic cuttings is strongly recommended to achieve quantitative and qualitative objectives. Some experiments reported that some Theobroma cacao clones produced by orthotropic cuttings do not root easily and the rate is very low, around 30 to 50% results....

Author(s): Arthur Tapi, Mahamadou Sangaré, Cheikh Mbacké Mboup and Kam-Rigne Laossi

April 2017

Genetic diversity in quarin clover (T. quartinianum) accessions of Ethiopia using ISSR markers

Genetic diversity of Trifolium quartinianum accessions from Ethiopia were studied using Inter Simple Sequence Repeat (ISSR) markers. A total of 24 accessions, divided into three populations were used for the present study. DNA was extracted from a bulk of samples using a modified CTAB method. A total of 84 bands were amplified by the four di-nucleotid ISSR primers in the overall experimental materials. Genetic diversity...

Author(s): Tadesse Abate and Kassahun Tesfaye

April 2017

Efficiency of sequence related amplified polymorphism (SRAP) and target region amplified polymorphism (TRAP) markers in detecting banana somaclonal variants

Screening of micropropagated banana (Musa spp.) cv. Grand Naine for somaclonal variants was carried out in the open field. The total detected variants were 26 somaclones (in addition to the normal plant) and were grouped into eight groups according to their morphological and yield characteristics. Sequence Related Amplified Polymorphism (SRAP) and Target Region Amplified Polymorphism (TRAP) techniques were used to study...

Author(s): Abdelhamid A. El-Shahed, Kamal F. Abdellatif, Ibrahim A. Ibrahim, Amany M. Mohamed, Inas Z. Abdelsalam and Osama A. Elsehrawy

April 2017

Genetic diversity of Ethiopian emmer wheat Triticum dicoccum Schrank landraces using seed storage proteins markers

Emmer wheat [Triticum dicoccum (Schrank)] cultivation in Ethiopia has been reducing and the genetic diversity maintained in the germplasm collection needs to be determined. The objective of this study was to assess the level of genetic diversity among populations of Ethiopian emmer wheat using seed storage protein analysis. Seeds of 85 accessions representing seven administrative regions of Ethiopia were obtained from...

Author(s): Shiferaw Alemu Alayachew and Kassahun Tesfaye Geletu

April 2017

Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers

Swertia chirayita is a highly valued but vulnerable medicinal plant species of Nepal. Its populations are declining in natural habitats due to over exploitation. Twenty-seven inter-simple sequence repeats (ISSR) primers were used to assess the genetic diversity and population genetic structure of 42 genotypes representing six natural populations of S. chirayita, from Nepal. Of the total 479 bands amplified by 27 ISSR...

Author(s): Surendra Neupane, Jaishree Sijapati, Tribikram Bhattarai and Sangita Shrestha

April 2017

Analysis of similarities within different date palm cultivars of Al-Ahsa oasis in Saudi Arabia

Date palm (Phoenix dactylifera L.) is widely cultivated in Saudi Arabia, with annual production estimated to be above one million tons. Of the 450 cultivars that are commonly used, 70 cultivars are found in the Eastern province and 20 cultivars are well known and common in the Al-Ahsa oasis. In this study, thirty seven date palm samples representing nine cultivars collected from six locations in the Al-Ahsa oasis, Saudi...

Author(s): Adil M. Al-Issa

April 2017

Enzootic bovine leukosis and the risk to human health

The bovine leukemia virus (BLV) was first reported as enzootic bovine lymphosarcoma in Eastern Europe in the late 19th century, highlighted by the presence of slightly yellow nodules in the enlarged spleen of cattle. It was believed to be an infectious disease because it spreaded through the herds. With changes observed in sensitivity of diagnostic techniques, the opinion that “BLV does not infect humans”...

Author(s): Axel Villalobos-Cortés

April 2017

In vitro anti-tyrosinase and anti-elastase activity of collagen from sea cucumber (Holothuria leucospilota)

Collagen is the main structural component of connective tissues in vertebral and invertebral animal tissues and organs. The collagen isolated from the body wall of sea cucumber (Holothuria lecospilota) can be used in the pharmaceutical field. The research aimed at isolating collagen from the sea cucumber body wall and testing the anti-tyrosinase and anti-elastase activity. This was done by isolation of collagen by...

Author(s): Syamsudin Abdillah, Gita Wijiyanti, Metta Setiawan, Siti Umroh Noor and Mala Nurilmala

April 2017

Production of invertase from Saccharomyces cerevisiae Angel using date syrup as a cost effective carbon source

Invertase (β-fructofuranosidase) finds major applications in food, cosmetics, and pharmaceutical industries. In the present study, fermentation conditions for invertase production from Saccharomyces cerevisiae Angel using date syrup in shake flask were investigated. The effect of time period (0-96 h), carbon sources (sucrose, date syrup, maltose, galactose, and glucose), nitrogen sources including yeast extract,...

Author(s): Abdul Sattar Qureshi, Imrana Khushk, Chaudhary Haider Ali, , Hamid Majeed and Ayyaz Ahmad

April 2017

Assessment of genetic diversity among cultivated Pearl millet (Pennisetum glaucum, Poaceae) accessions from Benin, West Africa

Simple sequence repeat (SSR) molecular markers were used for genetic diversity analysis and population structure of the cultivated Pearl millet in Benin, West Africa. In order to assess the level of genetic diversity, 14 polymorphic SSR markers were used to screen 114 accessions from different agro-ecological zones in Benin. SSR markers were found to reveal a total of 57 alleles with an average of 4.071 allele per...

Author(s): Kifouli ADEOTI, Gustave DJEDATIN, Ebenezer EWEDJE, Thierry BEULE, Sylvain SANTONI, Alain RIVAL and Estelle JALIGOT

April 2017

Enhancing the production of syringomycin E in Pseudomonas syringae pv syringae by random mutagenesis and molecular characterization of the SyrB1 gene

Syringomycin E (SRE) is a phytotoxin produced by Pseudomonas syringae pv syringae with high potential as a safe and effective therapy for the control of human fungal infections and as a preservative for the food industry. In this study, 27 strains of P. syringae pv syringae were isolated from plums, potatoes, apricots and peaches, of which P. syringae pv syringae 120 (PS120) showed the highest SRE production levels....

Author(s): Nguyen Hai Trieu, Nguyen Ngoc Huyen, Nguyen Huong Tra, Nguyen Thi Hong Ha, Harsharnjit S Gill, Nguyen Minh Huong and Dong Van Quyen,

April 2017

The impact of salinity stress on morphological and anatomical aspect of water hyssop Bacopa monnieri (L.) Wettst grown in vitro

This study was conducted at Marine Science Center, Basrah University, Basrah, Iraq during the period of 2015 to 2016. In the present work, we used NaCl and polyethylene glycol (PEG) to induced abiotic stress in vitro. Results show that high shoot numbers (24) were produced on MS (solid) medium enriched with 0.8 g/L NaCl in addition to cytokinins while, inclusion of drought agent PEG to proliferation medium (solid and...

Author(s): Abdulminam Hussien Ali Almusawi, Haleemah Jabbar Al-Aradi and  Kadhim Jasim Hammadi

April 2017

Effects of activated charcoal on medium-term conservation of yam (Dioscorea spp.) cultivated in Benin

The effects of activated charcoal were tested on medium term conservation of yams cultivated through tissue culture techniques. Galzy glutamine basic medium and that supplemented with 3 g/l activated charcoal were used. Growth parameters such as number of leaves, height of stem, number of nodes and length of the main root are evaluated on plantlets after 20 months. The test T of Student Newman and Keuls (SNK) with two...

Author(s): Adjahossou B. Sêdami, Cacaï Gilles Habib Todjro, Dangou Sossou Justine, Agbidinoukoun Arnaud, Marcos A. Lionel, Agbangla Clément and Ahanhanzo Corneille,

April 2017

Determination of proximate and mineral compositions of Moringa oleifera and Moringa stenopetala leaves cultivated in Arbaminch Zuria and Konso, Ethiopia

This study presents the proximate and mineral composition analysis of Moringa oleifera and Moringa stenopetala leaves collected from two different agro-ecological zones namely Arbaminch Zuria and Konso in Southern Nation Nationalities and Peoples Region (SNNPR), Ethiopia. M. oleifera and M. stenopetala leaves were collected using random sampling method from four sampling sites namely Secha, Karat, Sikela, Gato. Before...

Author(s): Debebe Mikore and Eyobel Mulugeta

April 2017

Biosynthesis of silver nanoparticles by endophytic fungi: Its mechanism, characterization techniques and antimicrobial potential

Nanotechnology has rapidly developed as an important field of modern research, generating most promising applications in electronics and medicine. It involves different approaches for the synthesis and application of nanoparticles having dimension smaller than 100 nm. Nanoparticles are the fundamental building blocks for preparing many nanostructured materials and devices. Therefore, there is an enormous interest in...

Author(s): Sardul Singh Sandhu, Harshita Shukla and Shyamji Shukla

April 2017

Effects of solid matrix priming with Trichoderma harzianum on seed germination, seedling emergence and photosynthetic capacity of eggplant

The effects of solid matrix priming with strain T22 of Trichoderma harzianum on seed germination, seedling emergence and photosynthetic capacity in two eggplant varieties were evaluated. Seed were primed by mixing with vermiculite and the T. harzianum wettable powder (WP) suspension at a rate of 1:3:3.2 (fresh weigh basis, respectively) at 15°C for 7 days in the dark, then primed seed were dried. Solid matrix...

Author(s): Lingyun WU, Dongwei YAO and Ming LI

April 2017

Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Rhodanese a ubiquitous sulphur transferase enzyme catalyses the detoxification of cyanide to a less toxic thiocyanate. Rhodanese from the root of Pentadiplandra brazzeana was purified by ammonium sulphate precipitation and affinity chromatography. The enzyme had a specific activity of 4.82 RU/mg of protein. The Km values of the substrates (KCN and Na2S2O3) were 11.76 and 10 mM, respectively. The optimum pH and...

Author(s): Raphael E. Okonji, Bamidele S. Fagbohunka, Leonard O. Ehigie, Zainab A. Ayinla and Olajumoke O. Ojo

April 2017

Quality characteristics of Korean traditional rice wine (Takju) prepared by addition of green pepper

Makgeolli, frequently called Takju, a famous Korean traditional rice wine, has long been prepared and consumed in Korea. Takju consumption in Korea has been increased because of its nutritional and functional values. The objective of the present study was to investigate the chemical characteristics, color values, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities, total phenolic contents, and sensory...

Author(s): Chan Yong Kim, Kil Su Jang, Su Gyeong Jeon, Sanjeev Kumar Dhungana, Hyeon Min Do, Oh Hun Kwon, Oh-Heun Kwon, Hye-Ryun Kim and Il-Doo Kim

April 2017

Evaluation of the role of SsaV ‘Salmonella pathogenicity island-2 dependent type III secretion system components on the virulence behavior of Salmonella enterica serovar Typhimurium

Salmonella is a causative agent of wide range of diseases varying from gastroenteritis to systemic typhoid fever. It uses specialized Type III secretion system (T3SS) by its two compartments to invade and intracellularly survive inside the immune cells. T3SS is expressed in two subsequent phases by two distinct Salmonella pathogenicity islands (SP) I and II. Understanding and evaluation of components T3SS-SPI1 and...

Author(s): Wael A. H. Hegazy, and Hisham A. Abbas

April 2017

Assessment of genetic diversity and structure of Sporisorium scitamineum from China using inter-simple sequence repeat (ISSR) markers

In this study, the genetic diversity and structure of 90 monosporidia mating-type isolates of Sporisorium scitamineum collected from China were comprehensively evaluated based on the DNA extracted from mating-type haploid sporidias using inter-simple sequence repeat (ISSR) polymorphism molecular marker technique. Fifteen selected ISSR primers produced 162 amplified DNA fragments, of which 115 (71.0%) were polymorphic....

Author(s): Gang-hong Xu, Quan-qing Deng, Wan-kuan Shen,, Shuang Chen, and Xia-ming Wu,

April 2017

First detection of deformed wing and kakugo viruses in honeybees (Apis mellifera L.) in Egypt by real time-polymerase chain reaction (RT-PCR)

The identification of bee viruses is of great importance where there is no information on their natural occurrence in honeybee populations. Honeybee samples (Apis mellifera L.) obtained from Giza Governorate, Egypt were evaluated for total RNA from the head, thorax and abdomen, and varroa mites. Two fragments of 250 and 540 bp of deformed wing (DWV) and kakugo viruses (KV), respectively, were amplified using...

Author(s): Emtithal M. Abd-El-Samie, Fatma K. Adham, Sawsan El-Mohandes and Heba Seyam

April 2017

Micropropagation of two species of Micranthocereus (Cactaceae) with ornamental potential native to Bahia, Brazil

The two target cactus species in this study, Micranthocereus flaviflorus subsp. densiflorus (Buining and Brederoo) P.J.Braun and Esteves and Micranthocereus. polyanthus subsp. alvinii M. Machado and Hofacker, are endemic to the state of Bahia and have ornamental value. Therefore, this work proposed to establish M. flaviflorus in vitro, as well as to micropropagate both species. The temperature regimes 25°C and...

Author(s): Laila Mandel Civatti, Maria Nazaré Guimarães Marchi and Moema Cortizo Bellintani

March 2017

Bioactivity of ethanolic extracts of Euphorbia pulcherrima on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is a polyphagous species which attacks many economically important crops in several countries. This insect is an important pest of corn, and currently the most widely used control method is chemical. In order to minimize environmental impacts, other forms of control have been tried, and accordingly, the investigation of plants with...

Author(s): Viviane Tavares Almeida, Vânia Maria Ramos,, Matheus Barbosa Saqueti, Pedro Henrique Gorni, Ana Cláudia Pacheco and Renato Marcos de Leão,

March 2017

Efficiency of the partial substitution of agar with potato starch on the growth and phytochemical parameters of lulo (Solanum quitoense) cultured in vitro

The demand for agar, thanks to its different uses, generates the need to look for alternative substances that replace it. This work studied the influence of four different additions of potato starch into agar media (T1 = 50%, T2 = 45%, T3 = 40% and T4 = 0%) on the growth and chemical composition of essential oils of lulo (Solanum quitoense) explants cultured in vitro. The lengths, fresh and dry biomass weights, and...

Author(s): D. A. Martin G., Oswaldo E. Cárdenas-González, José Pacheco, Maritza De Jesus-Echevarría and Jovanny A. Gómez Castaño

March 2017

Evaluation and association mapping of agronomic traits for drought tolerance in sorghum [Sorghum bicolor (L.) Moench]

Drought is the major sorghum production constraint in Ethopia which necessitates the identification of sorghum genotypes that carry genes (quantitative trait locus, QTL) associated with drought tolerance thereby developing drought tolerant sorghum varieties. The objectives of this study were to identify drought tolerant sorghum genotypes, map chromosomal regions (QTLs) associated with agronomically important traits and...

Author(s): Aleye Endre and Kassahun Bantte

March 2017

Isolation and characterization of heavy metals resistant Rhizobium isolates from different governorates in Egypt

Contamination of soil with heavy metals is one of the major environmental problems in many countries that reach from many sources as power stations, application of metal pesticides, fertilizers, and sewage sludge. In this study, ten Rhizobium leguminosarum bv. viciae isolates were collected from different governorates in Egypt (Menoufia, Kafr El-Sheikh, Qalubia, Fayoum, Ismailia, Sharkya, Dakhalia, Behira and North...

Author(s): Khalid S. Abdel-lateif,

March 2017

Morphometric characterization of Jatropha curcas germplasm of North-East India

The morphological variation among Jatropha curcas L. populations from 29 different locations of North-East India was determined. Four populations from other parts of India were also incorporated in the study as an out-group. The morphological trait based analysis of J. curcas revealed large variation of quantitative traits among the populations. Of the six morphological traits used in this study, the highest variation...

Author(s): Adreeja Basu, Lokanadha Rao Gunupuru and Lingaraj Sahoo,

March 2017

Effect of Brazil nut oil (Bertholletia excelsa HBK) on the physical, chemical, sensory and microbiological characteristics of a mayonnaise-type emulsion

The objective of this study was to evaluate the influence of emulsifiers on the chemicophysical, colorimetric, microscopic and sensorial properties, and the stability of a mayonnaise-type emulsion prepared with Brazil nut oil (Bertholletia excelsa H.B.K). For this purpose, two emulsifiers were used: a soya protein isolate and dehydrated and pasteurised egg yolk as a source of protein. Both formulations had high energy...

Author(s): Cristina Grace de Sousa Guerra, Jaime Paiva Lopes Aguiar, Wallice Luiz Paxiuba Duncan, Ariane Mendonça Kluckzosvki and Francisca das Chagas do Amaral Souza

March 2017

Phenolic compounds and antioxidant activity of red and white grapes on different rootstocks

This study aimed to assess the rootstocks influence over total phenolic compound content, antioxidant activity and its correlation in different red and white grapes cultivars for wine production. This study conducted an experimental three-year-old vineyard, located in Jundiai, in the State of São Paulo, Brazil, from July 2013 to January 2014. Red and white grapes from Vitis vinifera L. (Cabernet Sauvignon,...

Author(s): Marlon Jocimar Rodrigues da Silva, Bruna Thaís Ferracioli Vedoato, Giuseppina Pace Pereira Lima, Mara Fernandes Moura, Giovanni Marcello de Angeli Gilli Coser, Charles Yukihiro Watanabe and Marco Antonio Tecchio

March 2017

Isolation and identification of Talaromyces purpurogenus and preliminary studies on its pigment production potentials in solid state cultures

Pigments from various sources such as annatto seeds, cochineal, beet root, and microalgae are widely used in food, pharmaceutical, cosmetics, textile and other industries. However, these sources of pigments have various limitations such as toxicity and environmental pollution of synthetic pigments, and low productivity of pigments from higher organisms due to long period of growth. The objective of this study was to...

Author(s): Christiana N. Ogbonna, Hideki Aoyagi and James C. Ogbonna

March 2017

Microsatellite, inter simple sequence repeat and biochemical analyses of Rosa genotypes from Saudi Arabia

Rosa damascena Mill. as a main economic crop in the world is planted for beauty and essential oil production in Ta’if region. For the management and improvement of this important crop, genetic variability was evaluated amongst six Rosa genotypes grown in different plantations using microsatellite (simple sequence repeats, SSR), inter simple sequence repeat (ISSR) and biochemical markers. The six SSR primers showed...

Author(s): Shawkat M. Ahmed, Hadeer Y. Darwish, and Khalid H. Alamer

March 2017

PCR validation of predicted virulence factors in a collection of Xanthomonas campestris (pv. musacearum and vascolurum) strains

Bacterial plant pathogens belonging to the Xanthomonas genus are adapted to their host plants and are not known to colonize other environments. Xanthomonas colonize host parts such as leaves, stems and roots before entering vascular tissues and engaging in an invasive pathogenic phase. These bacterial strains have evolved strategies to adapt to life in this environment. The host-pathogen interactions of Xanthomonas...

Author(s): Arthur Wasukira, Geoffrey Tusiime and Jerome Kubiriba

March 2017

A study on metallo-β-lactamases producing Pseudomonas aeruginosa in water samples from various parts of Malaysia

Pseudomonas aeruginosa is a Gram negative aerobic rod shaped bacterium and is an opportunistic pathogen that usually causes nosocomial infection in immunocompromised patient with various infections and affects normal healthy human as well. P. aeruginosa is also an omnipresent pathogen that can be inhabited in soil, water, vegetable, human and animal. Metallo-β-lactamases (MBL) producing P. aeruginosa are known to...

Author(s): Nagaraja Suryadevara, Ooi Yi Shuang and Paulraj Ponnaiah

March 2017

Ethanolic Allium sativum extract down-regulates the pelF gene involved in Pseudomonas aeruginosa biofilm formation

Exploration of efficacious plant extracts that can reduce or inhibit Pseudomonas aeruginosa biofilm formation is necessary. Allium sativum is a suitable candidate because of its relative abundance. This study was carried out to determine the effect of ethanolic A. sativum extract on the expression of the P. aeruginosa biofilm gene, pelF. The presence of the pelF gene in the isolates used for this study was confirmed via...

Author(s): Nathaniel Nyakaat Ninyio, Hadiza Bashir Gidado, Mariama O. Yahaya, Tayaza  B. Fadason, Raji A. Bamanga and Lucy M. Yaki

March 2017

In vitro evaluation of lactic acid bacteria isolated from traditional fermented Shamita and Kocho for their desirable characteristics as probiotics

The study was conducted to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from fermented Shamita and Kocho. Sixteen samples, 8 each of Shamita and Kocho, were collected from Arat-Kilo and Merkato sites in Addis Ababa, respectively. The average pH values of Shamita and Kocho samples were 3.52 and 3.44, respectively. A total of 140 LAB were isolated, of which 101 isolates (72%) were found to...

Author(s): Negasi Akalu, Fassil Assefa and Asnake Dessalegn 

March 2017

Differentiation of Urochloa brizantha cultivars by inter-simple sequence repeat (ISSR) markers in seed samples

Approximately 80-90% of cultivated grassland areas in Brazil are covered by Urochloa brizantha [syn. Brachiaria brizantha (Hochst. ex A. Rich.) Stapf.]. Some genotypes of Urochloa have being widely used with a wrong nomenclature, like species and cultivars. In this way, the Urochloa cultivar identification is primordial for breeding programs and seed production. Considering the importance of genetic purity in...

Author(s): Inaê Braga, Claudia Jaqueline Tome Yamamoto, Ceci Castilho Custódio and Nelson Barbosa Machado-Neto

March 2017

Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

The actinobacteria are the most abundant bacterial group in the soil, where they present different characteristics and antagonistic effects on other microorganisms. The objectives of this study were to characterize and evaluate the antagonistic effect of actinobacteria from the semiarid region on rhizobia from the same climatic region. Tests were performed to observe chromogenic and micro-morphological characteristics,...

Author(s): José Vinícius Leite Lima⊃, Suzana Cláudia Silveira Martins⊃, Katia Aparecida de Siqueira⊃, Marcos Antônio Soares⊃ and Claudia Miranda Martins⊃

March 2017

Post-treatment of municipal sewer in shallow polishing ponds

This work aims to investigate the influence of polishing ponds (PP) depth on the post-treatment of sanitary sewers. Two treatment systems were designed and monitored. One system was an upflow anaerobic sludge blanket (UASB) rector and intermittent flow sand filter; followed by a PP of 0.20 m depth with low superficial application rate (24.9 kgBOD5.ha-1.day-1), high sunlight incidence (597 Wm-2) and enough biological...

Author(s): Tales Abreu Tavares de Sousa, Wilton Silva Lopes, Israel Nunes Henrique, Valderi Duarte Leite and José Tavares de Sousa

March 2017

Effect of two different habitats on some primary and secondary phytochemicals of Miswak (Salvadora persica L.)

Salvadora persica is a popular medicinal plant in the Middle East countries. It is well adapted to a wide range of habitats. In this study we have compared the effect of two different habitats (Al-ahsabah valley and Shada Mountain) in Al-Baha City, south west of Saudi Arabia on the content of some primary and secondary metabolites in S. persica. The results show that soil texture, soil moisture and organic matter...

Author(s): Amal Ahmed Mohammed Al-Ghamdi and Manal El-Zohri,

March 2017

Nested polymerase chain reaction (nPCR) based diagnosis of bovine leukemia virus in Panama

The bovine leukemia virus is an exogenous retrovirus that causes enzootic bovine leukosis. The aim of this study was to apply and compare a diagnostic test in an outbreak of bovine leukemia virus by nested polymerase chain reaction (PCR)  in a core conservation of native cattle Guaymí. From the results obtained by the three techniques used, the agar gel immunodiffusion (AGID) test detected 33 positive...

Author(s): Axel Villalobos-Cortés, Selma Franco, Rita Gonzalez and Marcelino Jaén

March 2017

Proteins patterns of eight genera of the Asteraceae family

Asteraceae family grows as wild plants in the eastern province of Saudi Arabia near the Gulf coast with prevalent high humidity coupled with more moderate temperatures. Eight genera were collected during the flowering season to study genetic diversity according to protein patterns for each plant. The protein patterns showed different numbers of bands, concentrations, molecular weight and intensity. Two common bands were...

Author(s): Mona S. Al-Ahmadi

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