African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12179

AJB Articles

January 2017

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Honey is a sweet viscous liquid produced by honey bee, Apis mellifera from the nectar of plants. Honey is a natural product that has been used from ancient times till now as food and for medicinal purpose. This study was carried out to determine the mode of action of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria. Bacilli spp. count was carried out by initially heating...

Author(s): Obakpororo E. Agbagwa, Swaroopa D. Rani and Prakash M. Halami

January 2017

Effects of regionally applied heating on the respiration of wild type and transgenic soybean (Glycine max) plants grown under ambient and elevated CO2 environments

Nocturnal dark respiration (Rn) in wild type and transgenic soybean plants grown at SoyFACE research facility, Illinois, USA under ambient and elevated CO2 conditions was examined in this study. Transgenic plants were transformed to overexpress a key Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBP) which is thought to improve yield in the field by at least 11%. Heating was applied using infrared heaters...

Author(s): Chukwuma Collins Ogbaga

January 2017

Protocol optimization for in vitro shoot multiplication of Jackfruit (Artocarpus heterophyllus L.)

Jackfruit (Artocarpus heterophyllus L.) is a cross pollinated fruit tree valued mainly for its fruit and timber wood. Although the crop has been introduced to Ethiopia and adapted well to the Jimma area, its potential for production has not been exploited due to the absence of an efficient method for large scale propagation. Micropropagation is a method for production of large number of genetically uniform planting...

Author(s): Jemal Ali, Kassahun Bantte and Tileye Feyissa

January 2017

Factors affecting reproductive performance of dairy cow in Algeria: Effects of clinical mastitis

The objective of the present study was to evaluate the effects of clinical mastitis at the beginning of lactation on reproductive performance of dairy cows in Algeria. Calving to first insemination and calving to conception intervals, number of insemination per conception and conception rate at the first artificial insemination were recorded for 432 cows from 16 dairy herds in Algeria. Cows were examined for clinical...

Author(s): Bouamra M., Ghozlane F. and Ghozlane M. K.

January 2017

Characterization and optimization of lipase activity produced by Pseudomonas monteilli 2403-KY120354 isolated from ground beef

A total of 56 Gram negative bacterial isolates were recovered from twenty ground beef samples and were screened for their potentiality to produce lipase. Forty four bacterial isolates were recorded as positive producers for lipase on tween as carbon source in solid medium. Also, the highly producer isolates were screened for lipase activity in submerged culture using olive oil as carbon and the most active isolate was...

Author(s): Abdel-Hamied M. Rasmey, Akram A. Aboseidah, Salha Gaber and Fatma Mahran

January 2017

Molecular detection of disease resistance genes to powdery mildew (Blumeria graminis f. sp. tritici) in wheat (Triticum aestivum) cultivars

A study was conducted to detect the presence of disease resistance genes to infection of wheat powdery mildew (Blumeria graminis f. sp. tritici) in selected wheat cultivars from China using molecular markers. Genomic DNA of sixty cultivars was extracted and tested for the presence of selected prominent resistance genes to the fungal disease using molecular markers linked to Pm genes. Results showed that 17 cultivars...

Author(s): Vincent Mgoli Mwale, Xiuli Tang and Eric Chilembwe

January 2017

Extraction and characterisation of gelatin from the skin of striped catfish (Pangasianodon hypophthalmus) and studies on its colour improvement

Functional properties of gelatin from skin of striped catfish with and without bleaching for 48 h by 5% H2O2 (w/v)) were studied. Gelatin from skin bleached with 5% H2O2 for 48 h showed the highest yield (16.18 g). Bleaching not only improved the colour of gelatin gel by increasing the L* (lightness)-value and decreasing a*(redness/greenness)-value, but also enhanced the bloom strength and the emulsifying and foaming...

Author(s): Prabjeet Singh, and Soottawat Benjakul

January 2017

Electrolyte ions and glutathione enzymes as stress markers in Argania spinosa subjected to drought stress and recovery

Understanding the mechanisms underlying Argania spinosa responses to drought stress is essential for its regeneration and domestication. Toward that end, an integrative study of tolerance responses to drought stress in four A. spinosa ecotypes (2 contrasting coastal ecotypes (Adm and Rab) and 2 contrasting inland ecotypes (Alz and Lks)) have been conducted. Responses to soil drying and re-watering were measured at...

Author(s): Abdelghani Chakhchar, Mouna Lamaoui, Salama Aissam, Abderrahim Ferradous, Said Wahbi, Abdelhamid EI Mousadik, Saad Ibnsouda-Koraichi, Abdelkarim Filali-Maltouf and Cherkaoui El Modafar

January 2017

Isolation and characterization of heavy metal tolerant bacteria from Panteka stream, Kaduna, Nigeria and their potential for bioremediation

Panteka stream is a flowing stream polluted with wastes from the activities of mechanics. Water samples collected at different points of the stream were analysed in order to determine the level of heavy metal contamination and bacteria diversity with the view to elucidating the bioremediating potentials of the bacteria isolates. Four bacteria, tolerant to heavy metals, were isolated from Panteka stream. These were...

Author(s): Elizabeth Chika Nwagwu, Victoria Moltong Yilwa, Nkechi Eucharia Egbe and Godwin Bryan Onwumere

January 2017

Generation of bioethanol from common date by-products, “Teggaza and Lebghel” in Southern Algeria

Date by-products constitute the principal food for the oasis populations in Middle East and North Africa. Dates contents consist of 70 to 80% of reducing sugars, and do not require an intensive energy and labour for thermophysical pre-treatment. They can serve as a good feedstock for bioethanol generation through fermentation and distillation. Algeria is among the top sixth producers of dates in the world with more than...

Author(s): Ahmed Boulal, Mabrouk Kihal, Cherif Khelifi and Boudjemâa Benali

January 2017

Comparison of growth and yield adaptability indicators of two maize (Zea mays L.) cultivars under planting basin technique in Zimbabwe

In eastern semi-arid Zimbabwe, planting basins are mostly used to boost maize yields, but still low yields are often obtained due to poor choices of varieties to grow. A comparative study of growth and yield of the only two locally and commonly grown Pioneer cultivars (P2859W and PHB3253) under planting basin technique (PBT) was carried out to determine a more adaptable cultivar in Guhune, eastern Zimbabwe (NR IV). The...

Author(s): Stephen Mutungwe Culver Mvumi and Sekai Annastasia Manyiwo

December 2016

Optimizing Bacillus circulans Xue-113168 for biofertilizer production and its effects on crops

In this study, Bacillus circulans Xue-113168 biofertilizer was produced through solid state fermentation processes using food waste and feldspar. Results confirmed that solid state fermentation has considerable advantages compared to complex process (solid-state and bio-bleach). The control of pH, temperature, and humidity effectively led to the formation of 2×109 cfu/g spore and dissolution of potassium at a rate...

Author(s): Shengping Xue, Liantian Miao, Yujie Ma, Yan Du and Hongbo Yan

December 2016

Effect of solarization with fresh chicken manure on verticillium wilt (Verticillium dahliae Klebb) and yield in eggplant

The present study was carried out to evaluate the effect of reducing wilt disease through the medium of fresh chicken manure (FCM) mixed with soil before solarized and then artificial Verticillium dahliae (V.d) inoculation on yield of eggplant (Solanum melongena L.) under field conditions. According to the split-plot design, solarization of main plots, sub-plots with fresh chicken manure and V dahliae inoculation were...

Author(s): Ismail Cimen and Mahir Basaran

December 2016

Nucellar embryogenesis and plantlet regeneration in monoembryonic and polyembryonic mango (Mangifera indica L.) cultivars

Biotic and abiotic stress particularly fungal diseases and salinity are major challenges facing mango cultivations in Oman. Micropropagation technique for multiplying disease resistant and salinity tolerant elite cultivars could be utilized to replace dead and infected plants in mango orchards but standardize in-vitro regeneration protocol via somatic embryogenesis is prerequisite. Nucellar tissues from immature mango...

Author(s): Manish Shukla, Khair Tuwair Al-Busaidi, Ghaya Sultan Al-Blushi, Abdullah Hamed Al-Burashdi, Hamoud Darwish Al-Hasani, Mohammed Hamed Al-Jabri and Basem Saif AL-Kalbani

December 2016

Genetic diversity and population structure of common bean (Phaseolus vulgaris L) germplasm of Ethiopia as revealed by microsatellite markers

The Ethiopian genetic center is considered to be one of the secondary centers of diversity for the common bean. This study was conducted to characterize the distribution of genetic diversity between and within ecological/geographical regions of Ethiopia. A germplasm sample of 116 landrace accessions was developed, which represented different common bean production ecologies and seed types common in the country. This...

Author(s): Zelalem Fisseha, Kassahun Tesfaye, Kifle Dagne, Matthew W. Blair, Jagger Harvey,  Martina Kyallo, Paul Gepts

December 2016

Acute and sub-chronic toxicity study of the extract and powder of Operculina macrocarpa (L.) Urb. in mice

The aim of the study was to evaluate the experimental acute and sub-chronic toxicities of Operculina macrocarpa with plant extract and powder, respectively, in male and female mice. Phytochemical prospection was performed with extract and administration in single doses by intraperitoneal route to six groups and control at 1230, 970, 700, 350, 120 and 30 mg/kg doses and distilled water (0.1 ml/10 g), respectively....

Author(s): Carpejane Ferreira da Silva, Ana Célia Rodrigues Athayde, Marcílio Fontes Cezar, Onaldo Guedes Rodrigues, Sergio Santos Azevedo, José Galberto Martins da Costa and Fabrício Kleber de Lucena Carvalho

December 2016

Ex vivo trypanostatic effect of stem-bark extracts of Securidaca longipedunculata (Fres. Holl) against Trypanosoma brucei brucei

Current treatment of trypanosomosis in sub-Saharan Africa is associated with widespread inefficiency. There is therefore the need to find more effective drugs against the disease from promising traditional medicinal herbs. This work is aimed at evaluating ex vivo anti-trypanosomal effect of stem-bark extracts of S. longipedunculata against T. brucei brucei. One hundred microlitre of crude methanol, ethyl acetate and...

Author(s): Abdullah Mohammad Tauheed, Mohammed Musa Suleiman, Mohammed Mamman, and Idris Alao Lawal

December 2016

Performance of rapid subtyping tools used for the classification of HIV type 1 recombinants isolated from selected countries in west and central Africa

HIV-1 genetic diversity in sub-Saharan Africa is broad and the AIDS epidemic is driven predominantly by recombinants in Central and West Africa. The classification of HIV-1 strains is therefore necessary to understand diagnostic efficiency, individual treatment responses as well as options for designing vaccines and antiretroviral (ARV) treatment guidelines. More so, accurate subtyping of a partial or full genome would...

Author(s): Bimela Jude Saber, Judith Ndongo Torimiro, Atogho Tiedeu Barbara, Tchapda Arlette Laure, Joseph Fokam, Desire Takou and Wilfred Fon Mbacham,

December 2016

Influence of sweeteners in the biodistribution of radiopharmaceutical and laboratory tests in rats

This study aimed to evaluate the effect of supplementation of sucralose and fructose on the metabolism of adolescent rats. Eighteen male Wistar rats were divided into 3 groups: control group (GC), fructose group (GF) treated with 50 mg/kg of fructose, and sucralose group (GS) receiving 50 mg/kg of sucralose for 24 days. The weight and feed intake were measured weekly. At the end of the experiment, some biochemical...

Author(s): Michelly Pires Queiroz, Vanessa Santos de Arruda Barbosa, Cecília Maria de Carvalho Xavier Holanda, Janette Monroy Osório, Tarciso Bruno Montenegro Sampaio, Christina da Silva Camillo, Aldo Cunha Medeiros, Marília Ferreira Frazão Tavares de Melo and Juliana Késsia Barbosa Soares

December 2016

Postharvest physicochemical properties of cucumber fruits (Cucumber sativus L) treated with chitosan-lemon grass extracts under different storage durations

The present study was carried out to evaluate the effects of chitosan-based edible coatings with lemon grass extract on the physical properties and overall acceptability of cucumber fruits stored at ambient temperature (28 ± 2°C) and 85 to 90% relative humidity for 14 days. Blends of chitosan (C) and lemon grass extract (E) were formulated as 0.5% C + 0.5% E, 0.5% C + 1% E, 1% C + 0.5% E and 1% C + 1% E....

Author(s): Olufunmilayo Sade Omoba and Uzoma Onyekwere

December 2016

Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

Clonal multiplication of oil palm enables the formation of commercial plantations with higher yield, which is possible through somatic embryogenesis. However, due to different modifications made in the existing protocols to avoid the appearance of the “mantled flower”, the success of this technique has been limited. Thus, this study aimed to apply a cloning protocol, using somatic embryogenesis, to 32 elite...

Author(s): Thais Roseli Corrêa, Sérgio Yoshimitsu Motoike, Ana Paula de Souza Andrade, Sara Morra Coser, Vanessa Queiroz, Manuela Maria Cavalcante Granja, Débora Durso Neves Caetano, Cristian Nicolás Mendoza Peña and Edgard Augusto de Toledo Picoli

December 2016

Identification of a novel submergence response gene regulated by the Sub1A gene

Submergence is one of the major constraints to rice production in many rice growing areas in the world. The Sub1A gene has been demonstrated to dramatically improve submergence tolerance in rice. Here, we report the identification of a novel submergence response (RS1) gene that is specifically induced in the Sub1A-mediated submergence tolerance response. Under submergence, RS1 was upregulated in M202 (Sub1A) but...

Author(s): Hewei Du,, Min Huang, Jianxiong Zhu, Hang Su and Xiu Zeng

December 2016

Investigation of Argania spinosa L. (Skeels) polyphenols growing in arid and semi-arid conditions

Argania spinosa L. Skeels, belonging to the Argania genus of the Sapotaceae family, is a species native to Morocco and Algeria.  Due to its perfect adaptation to soil and climate, this tree plays an important ecological role in a constantly threatened encroached desert region. To understand the biological role of polyphenols in making the argan tree adapts to its natural habitat, we conducted a comparative study in...

Author(s): Souad Djied, Saida Danoune, Jacqueline Grima-Pettenati Amina Belhandouz and Meriem Kaid-Harche

December 2016

Evaluation of the anti-inflammatory properties of the hexane extract of Hydrocotyle bonariensis Comm. Ex Lam. leaves

This study investigates the anti-inflammatory properties of Hydrocotyle bonariensis Comm. Ex Lam, a medicinal plant used by indigenous traditional healers to manage chronic inflammatory diseases especially rheumatism and arthritis. The hexane extract of H. bonariensis leaves was evaluated for the presence and concentration of phytochemicals. It was subjected to heat-induced albumin denaturation and human red blood cell...

Author(s): Obaseki, O. E., Adesegun, O. I., Anyasor, G. N. and Abebawo, O. O.

December 2016

Occurrence of Escherichia coli in Brassica rapa L. chinensis irrigated with low quality water in urban areas of Morogoro, Tanzania

Low quality water has become valuable resource with restricted or unrestricted use in food production depending on its quality. This study has quantified the occurrence of Escherichia coli in Brassica rapa L. chinensis (Chinese cabbage) vegetables and low quality irrigation water. A total of 106 samples including Chinese cabbage (69) and water (37) were collected. The E. coli were cultured in petri film selective E....

Author(s): Ofred J. Mhongole, Robinson H. Mdegela, Lughano J. M. Kusiluka and Anders Dalsgaard

December 2016

Application of polymeric nanoparticles for controlled release of ethanolic extract of guapeva leaves (Pouteria gardneriana Radlk) against Riphicephalus (Boophilus) microplus through in vitro studies

The study was designed to evaluate the ethanolic extract acaricide action from Pouteria gardneriana Radlk specie adsorbed in chitosan nanoparticles for controlled release against Riphicephalus (Boophilus) microplus. Leaves of P. gardneriana Radlk were collected, air-dried, ground and extracted with ethanol. Chitosan nanospheres were obtained by the phase inversion method. Conductometric titration, UV-Vis and FTIR...

Author(s): Priscila Fernanda Pereira Barbosa, Pablo Peres de Mendonça, Rômulo Davi Albuquerque Andrade, Ana Carolina Ribeiro Aguiar, Andréa Rodrigues Chaves, Adilson Ben da Costa and  Fabiano Guimarães Silva

December 2016

Allelopathic effect of aqueous extract of fresh leaf castor beans (Ricinus communis L.) applied to the beginning stage of soy (Glycine max L.) and safflower (Carthamus tinctorius L.)

Allelopathy defines the production of specific biomolecules (allelochemical) by a plant that can induce positive or negative impacts on another culture. The crop of castor beans (Ricinus communis L.) is being economically valued and receiving attention, mainly by the biodiesel production, castor oil and animal feeding. In this study, the allelopathic effect of aqueous extract from fresh leaves of castor beans (Ricinus...

Author(s): Renathielly Fernanda da Silva, Rodrigo Techio Bressan, Bruno Meneghel Zilli, Maurício Antônio Pilatti, Samuel Nelson Melegari de Souza and Reginaldo Ferreira Santos

November 2016

Thermo-chemical sequestration of naphthalene using Borassus flabellifer Shell activated carbon: Effect of influencing parameters, isotherm and kinetic study

The present research describes the removal of naphthalene from aqueous solution using one of the simplest agricultural wastes, Borassus flabellifer Shell activated carbon (BFS-AC) by adsorption. Adsorption was chosen due to its cost-effectiveness. The effect of operating parameters such as pH, time, initial concentration of naphthalene, BFS-AC dosage and temperature on the percent removal of naphthalene was determined....

Author(s): J. Aravind Kumar, D. Joshua Amarnath, S. Anuradha Jabasingh and S.Sathish

November 2016

Evaluation of insulin-like growth factor-I gene polymorphism in Egyptian small ruminant breeds

The genetic improvement of production traits can be developed through marker assisted selection. Insulin-like growth factor I (IGF-I) is a member of the somatotrophic axis which has a remarkable variation of its biological effect including protein synthesis and skeletal growth. This study aimed to detect the genetic polymorphism of IGF-1 in different Egyptian sheep and goat breeds. The amplified fragments at 320-bp were...

Author(s): Othman E. Othman, Mohamed F. Abdel-Samad and Nadia A. Abo El-Maaty

November 2016

Effect of organic matter, irrigation and soil mulching on the nutritional status and productivity of okra (Abelmoschus esculentus L.) in the semiarid region of Brazil

Okra (Abelmoschus esculentus L) is an important vegetable crop currently cultivated in many parts of the world. The adoption of management strategies to promote the nutritional status and crop productivity in semiarid regions of Brazil is still poorly studied. Thus, this experiment was conducted to evaluate the effect of raising the level of organic matter in the soil, irrigation and soil mulching in the nutritional...

Author(s): Marcelo de Andrade Barbosa, Núbia Marisa Ferreira, Antônio Michael Pereira Bertino, Evandro Franklin de Mesquita, Lucia Helena Garófalo Chaves, Lourival Ferreira Cavalcante and Everlon Cid Rigobelo

November 2016

Growth promotion mediated by endophytic fungi in cloned seedlings of Eucalyptus grandis x Eucalyptus urophylla hybrids

Eucalyptus cultivation has expanded considerably in Brazilian systems, leading to the current search for technologies to optimize growing conditions and the production of seedlings in nurseries. Based on the understanding that the development of tree species such as Eucalyptus sp. can be influenced by endophytic fungi that act directly as plant growth-promoting species, cloned seedlings of Eucalyptus grandis x...

Author(s): Luciana Cristina Vitorino, Layara Alexandre Bessa, Ligia Guimarães Carvalho and Fabiano Guimarães Silva

November 2016

Primary study on the components and main physico-chemical as well as biological properties of the oil of Alpinia galanga (L.) Wild in Phu Tho-Vietnam

The oil of Alpinia galanga (L.) Willd in Phu Tho was obtained by steam distillation and dried with Na2SO4. By Gas chromatography-mass spectrometry (GC-MS) method, 29 components in the oil were predicted by comparing their retention times and molecular weights with the standards’. In particular, there were 10 hydrocarbons such as monoterpenes: 29.15%, sesquiterpenes: 21.06%, and 19 oxygenated components like...

Author(s): Nguyen Van Loi and Nguyen Quynh Uyen

November 2016

A review of cashew (Anacardiumoccidentale L.) apple: Effects of processing techniques, properties and quality of juice

A review including the processing techniques, properties and uses of cashew apple juice is reported. Cashew apple has multi-purpose; it can be processed to obtain human food. The process of cashew apple into several by-products can affect its nutritional, microbiological, and sensorial quality attributes. Therefore, clarification methods, thermal treatment and high hydrostatic pressure modify nutritional,...

Author(s): Emmanuelle Dedehou, Joseph Dossou, Victor Anihouvi and Mohamed M. Soumanou

November 2016

Effect of whey storage on physicochemical properties, microstructure and texture profile of ricotta cheese

The aim of this study is to evaluate the effect of whey storage period (0, 24, 48 and 72 h) on the physicochemical parameters, color, texture and microstructure of fresh ricotta during storage. Sweet whey and acid whey were evaluated based on titratable acidity, pH, fat, cryoscopy, and density, while ricotta was based on yield, fat, protein, ash, acidity, pH, moisture, total solids, color, texture, and microstructure....

Author(s): Jéssica Leal Freitas Souza, Marco Antônio Pereira da Silva, Rânio Cesar Francisco da Silva, Ruthele Moraes do Carmo, Rodolfo Gomes de Souza, Juliana Aparecida Célia, Kênia Borges de Oliveira, Geovana Rocha Plácido, Moacir Evandro Lage and Edmar Soares Nicolau

November 2016

Influence of vermicompost humic acid on chlorophyll content and acclimatization in banana clone, Enano Guantanamero

Vermicompost humic acids (VHA) promote plants’ growth because they have similar effects with auxins. The aim of this research was to evaluate the effect of VHA in some physiological indicators in the micropropagation and acclimatization phase of banana clone Enano Guantanamero. Six concentrations were used (0, 10, 20, 30, 40 and 50 mg L-1) to evaluate in vitro the number of leaves, total chlorophyll content and...

Author(s): Marcia Beatriz Moya Fernández, Esteban Sánchez Chávez, Daniel Cabezas Montero, Andrés Calderín García, Dany Marrero López, Eduardo F. Héctor Ardisana and Sandra Pérez Álvarez

November 2016

Study of genetic determinism of harvest index in durum wheat (Triticum durum Desf) under semi-arid conditions

Out of six varieties of durum wheat (Triticum durum Desf.), two local varieties with a low harvest index and four others with high harvest indices and short straw imported from France were studied in a diallel cross. The experiment was done in a complete randomized block design with three replications. It was done at the Research Station of the Institute of Technical Big Cultures of Khemis Miliana, located in the...

Author(s): Bousalhih, B., Mekliche, L., Aissat, A. and Sadek Benabbes Halim

November 2016

Comparative phylogenetic analysis of intergenic spacers and small subunit rRNA gene sequences of two microsporidian isolates from Antheraea myllita

Two microsporidian isolates extracted from infected tasar silkworms (Antheraea mylitta)  collected from forest area in Deoghar district, Jharkhand, India were subjected to PCR amplification using intergenic spacer (IGS) region and small subunit rRNA (SSU-rRNA) gene specific primers followed by cloning and sequencing. The IGS and SSU-rRNA gene sequences were analysed to derive the identity of the microspoidian...

Author(s): Wazid Hassan, B. Surendra Nath and Geetha N. Murthy

November 2016

Isolation and characterization of methanogenic bacteria from brewery wastewater in Kenya

The production of biogas from renewable resources is becoming a prominent feature of most developed and developing countries of the world. A study was undertaken to characterize methanogenic microbial community found in brewery waste water. Their performance with regards to methane production was also studied. Thirty-two isolates were obtained using brewer thyglycollate agar medium. Characterization of the isolates was...

Author(s): Sylvia Injete Murunga, Duncan Onyango Mbuge, Ayub Njoroge Gitau, Urbanus Ndungwa. Mutwiwa and Ingrid Namae Wekesa

November 2016

Production of alkaline proteases by alkalophilic Bacillus subtilis during recycling animal and plant wastes

The production of extracellular alkaline protease by Bacillus subtilis was studied with submerged fermentation. A new strain of Bacillus sp. was isolated from alkaline soil, which was able to produce extracellular alkaline protease. The production of alkaline protease involved the use of agricultural or animal wastes at pH 8 and temperatures at 37°C. Results showed that growing B. subtilis sub sp. subtilis under...

Author(s): Amira Hassan Al-Abdalall and Eida Marshid Al-Khaldi

November 2016

Investigating the potential of Aucoumea klaineana Pierre sapwood and heartwood wastes to produce cellulosic ethanol

This work investigates for the first time the different reactivity exhibited by sapwood and heartwood wastes under two conditions: They were pretreated with catalyzed ethanol organosolv in the presence of sulfuric acid and their pulps underwent enzymatic hydrolysis by Trichoderma reesei which causes them to release neutral sugars for the production of cellulosic ethanol.  Aucoumea klaineana Pierre (Okoumé)...

Author(s): Rodrigue Safou-Tchiama, Sébastien Ngwa Obame, Nicolas Brosse, Patrice Soulounganga and Timoléon Andzi Barhé

November 2016

Comparison of seed priming techniques with regards to germination and growth of watermelon seedlings in laboratory condition

Seeds of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai, cv. Crimson Sweet] were used to investigate the effects of different priming techniques on seed germination and early seedling growth. The seeds were soaked in solutions of 0.2% gibberellin (50 mg L–1 gibberellic acid), 0.2% cytokinin (90 mg L–1 kinetin), 0.2% potassium nitrate (2 g L–1 KNO3), 0.2% calcium nitrate [2 g L–1...

Author(s): Willams Ferreira Souza Barbosa, Fábio Steiner, Leandro Castro Matias de Oliveira and Paulo Henrique and Menezes das Chagas

November 2016

Linkage disequilibrium and association mapping of drought tolerance in cotton (Gossypium hirsutum L.) germplasm population from diverse regions of Pakistan

Drought stress is a major abiotic stress that limits crop production. Molecular association mapping techniques through linkage disequilibrium (LD) can be effectively used to tag genomic regions involved in drought stress tolerance. With the association mapping approach, 90 genotypes of cotton Gossypium hirsutum, from diverse regions of Pakistan were used. The morpho-physiological traits of all genotypes were evaluated...

Author(s): Abdelhafiz Adam Dahab, Muhammad Saeed, Nada Babiker Hamza, Bahaeldeen Babiker Mohamed and Tayyab Husnain

November 2016

Inbreeding depression in crosses of coerulea clones of Walker’s Cattleya (Cattleya walkeriana Gardner)

Orchids are among the most beautiful flowers and endangered due to habitat destruction and over-collection. Cattleya walkeriana is one of the most beautiful flowers joining the small sized plant with medium large and heavily scented flowers. It is widely known and appreciated by its beautiful clones and it has much to offer to breeders because their plants have besides other attributes as small habit and big flowers,...

Author(s): Miléia Ricci Picolo, Ceci Castilho Custódio, Nelson Barbosa Machado-Neto

November 2016

The protective role of honey against cytotoxicity of cadmium chloride in mice

The present study aimed to investigate the honey (HY) protective role in opposition to cadmium (Cd) induced chromosomal aberrations of bone marrow and sperm abnormalities. Forty five (45) adult male albino mice were caged into six groups. Mice were injected, i.p, 300 mg HY/kg and/or 0.67 mg CdCl2/kg b.w for 96 h, separately and alternated. The alternated trials were continued for consecutive eight days. Results show...

Author(s): Asmaa Shahrour, Mohamed Zowail and Khaled Sharafeldin

November 2016

Shelf life improvement of sorghum beer (pito) through the addition of Moringa oleifera and pasteurization

Pito is a traditional alcoholic beverage that is mostly brewed in the three northern regions of Ghana. Although widely consumed and used in many festivities, poor storability limits its economic potential as an income-generating venture for most women. This study was carried out to improve the shelf-life of pito through the addition of Moringa oleifera leaf extract and pasteurization (75 to 80°C). Microbial...

Author(s): Florence Adwoa Ayirezang, Courage Kosi Setsoafia Saba, Francis Kweku Amagloh and Hellie Gonu

November 2016

Characterization of mutant cowpea [Vigna unguiculata (L) Walp] lines using random amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphism (AFLP) markers

Phylogenetic relationship and polymorphism was detected in 10 cowpea lines comprising of leaf, flower and stem mutants, their putative parents and an exotic accession using 10 random amplified polymorphic DNAs (RAPDs) and three primer combinations of amplified fragment length polymorphism (AFLP) markers. These mutants were earlier obtained through the probable activities of transposable elements. The RAPD and AFLP...

Author(s): Olufisayo Atinuke Kolade, Mercy Oluremi Olowolafe and Iyiola Fawole

November 2016

Pan-genome analysis of Senegalese and Gambian strains of Bacillus anthracis

Bacillus anthracis is the causative agent of anthrax, and it is classified as “category A” biological weapon. There were six available complete genomes (A0248, Ames, Ames Ancestor, CDC684, H0491 and Sterne). Here, one Gambian and two Senegalese strains (Gmb1, Sen2Col2 and Sen3) were added. In this work, the pan-genome of B. anthracis was studied based on nine strains and using bioinformatics tools as Cluster...

Author(s): M. Mbengue, F. T. Lo, A. A. Diallo,  Y. S. Ndiaye, M. Diouf and M. Ndiaye

November 2016

RNAi-based silencing of genes encoding the vacuolar-ATPase subunits a and c in pink bollworm (Pectinophora gossypiella)

RNA interference is a post- transcriptional gene regulation mechanism that is predominantly found in eukaryotic organisms. RNAi demonstrated a successful gene silencing in insects that led to the development of novel approaches for insect pest management. In the current study, genes encoding vacuolar ATPase (V-ATPase) subunits a and c from the midgut of pink bollworm, Pectinophora gossypiella, were cloned and sequenced....

Author(s): Ahmed M. A. Mohammed

November 2016

Genetic analysis of body weight of Takifugu rubripes at different developmental stages

To elucidate the genetic mechanism of growth trait in Takifugu rubripes during ontogeny, developmental genetic analysis of body weight was conducted by mixed genetic model with additive-dominance effects, using complete diallel cross with three different strains of T. rubripes from Laizhou Shandong, Tangshan Hebei and Dalian Liaoning. Unconditional genetic analysis revealed that the unconditional dominance effects with...

Author(s): Aijun Ma,, and Xin’an Wang,,

November 2016

Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia

This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry,...

Author(s): Hary Suhada, Sarbaini Anwar, Arnim, Hendri Maulana and Dan Yurnalis

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