African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5146

AJMR Articles

Isolation and characterization of some gut microbial symbionts from fungus-cultivating termites (Macrotermes and Odontotermes spp.)

July 2016

Microbiota of termites is crucial for nitrogen cycle activities and degradation of recalcitrant components of plant biomass that influence soil structure and carbon mineralization in tropical and subtropical regions. The aim of this study was to isolate and characterize gut bacteria that may be potentially associated with nitrogen metabolism from two fungus-feeding termites (Macrotermes and Odontotermes spp.). Twenty...

Author(s): Edith M. Muwawa, Nancy L.M. Budambula, Zipporah L. Osiemo, Hamadi I. Boga, and Huxley M. Makonde

Efficacy of Rhodopseudomonas containing multi-microbe probiotic on growth performance, mortality and cecal microflora in broilers

July 2016

Combinations of probiotic microorganisms are the study of interest due to beneficial impact through synergistic actions. Several researchers including our previous result found positive effects of various combinations of probiotic inclusion in broiler diet. In the current study, the efficacy of combinations of probiotic organisms by adding Rhodopseudomonas spp. was the primary interest on growth performance, mortality,...

Author(s): A. B. M. Rubayet Bostami, S. T. Ahmed, H. S. Mun, S. B. Hong and Chul-Ju Yang

An evaluation of the use of probiotics and manure composting as strategies to reduce levels of Shiga toxin-producing Escherichia coli in sheep

July 2016

Healthy ruminants appear to be the main reservoir of Shiga toxin-producing Escherichia coli (STEC). Importantly, this pathogen is shed in faeces of sheep and can cause outbreaks of human illness ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) caused by Shiga toxin-producing E. coli (STEC) have been reported worldwide. The manure of ruminants when used as agricultural fertilizer can...

Author(s): Everlon Cid Rigobelo, Marita Vedovelli Cardozo, Fernando Antonio de Avila and Patrick Joseph Blackall

Antibacterial properties of wild edible and non-edible mushrooms found in Zimbabwe

July 2016

Mushrooms have been used extensively in traditional medicine as antimicrobial, antiviral and antitumor agents. Infectious diseases remain a major threat to human health, due to global antimicrobial resistance. This has led to an increase in the search for new and potent antimicrobial substances. The aim of the present study was to investigate the antimicrobial activity of the aqueous (cold and hot) and organic solvents...

Author(s): Tsungai Reid, Chenjerayi Kashangura, Catherine Chidewe, Mudadi Albert Benhura and Takafira Mduluza,

Production of food colourants by filamentous fungi

July 2016

Food colourants are pigments or dyes added to food to maintain, intensify or add colour to foods. Although, the initial natural sources of food colourants were plants and animals, these sources have become inadequate due to increase in demand. This led to the use of synthetic colourants, some of which have harmful effects on human. Filamentous fungi are good sources of colourants since they are capable of synthesizing...

Author(s): Christiana N. Ogbonna

Molecular characterization of midgut bacteria of Aedes albopictus exhibiting swarming motility property

July 2016

Aedes mosquitoes are responsible for the transmission of arboviruses including dengue, chikungunya, zika, and yellow fever throughout the world. Mosquito gut harbors a variety of microbes, known to play a potential role in various physiological processes such as food digestion and pathogen development. Many bacterial species exert various movements like swarming, gliding and twitching which vary with individual...

Author(s): Kamlesh K. Yadav, Ashok Naglot, Kshitij Chandel, Sameer Bhagyawant, Ajitabh Bora, Hemant K. Gogoi, Vijay Veer and P. Srinivas Raju

β-Fructofuranosidase production by Aspergillus versicolor isolated from Atlantic forest and grown on apple pomace

July 2016

This study explores the production and characterization of an extracellular β-fructofuranosidase (FFase-I) by Aspergillus versicolor newly isolated from Atlantic Forest-Brazil. The β-fructofuranosidase production by fungus, after the optimization process using central composite design and response surface methodology, showed that 3% (w/v) apple pomace, an initial pH 7.5, and 12 days of cultivation provided the...

Author(s): Taiomara Butkze Dapper, Vanessa Cristina Arfelli, Caroline Henn, Márcia Regina Simões, Marise Fonseca dos Santos, Carla Lieko Della Torre, Jose Luis da Conceição Silva, Rita de Cássia Garcia Simão and Marina Kimiko Kadowaki

Characterization and purification of bacteriocin produced by Enterococcus sp. GHB26 isolated from Algerian paste of dates "Ghars"

July 2016

Strain Enterococcus sp. GHB26, isolated from the Algerian paste of dates "Ghars", produced a bacteriocin. This bacteriocin was inactivated by proteolytic enzymes. Antibacterial activity of the bacteriocin was heat stable at 120°C for 20 min (533 AU/ml), stable at pH range of 2 to 12 and resistant to chemicals (SDS, EDTA, NaCl, Tween 80, Urea). The active bacteriocin from the cell-free supernatant of...

Author(s): Amel Kout, Fatiha Dalache, Halima Zadi-Karam and Nour-Eddine Karam

Inhibition of biofilms on urinary catheters using immobilized Lactobacillus cells

July 2016

Bacterial biofilms forming on indwelling urinary catheters continue to represent a public health problem because they are associated with urinary tract infections (UTIs). This study was undertaken to evaluate the ability of immobilized Lactobacillus acidophilus cells to inhibit biofilm formation on catheter surfaces. Urine bacteria and Lactobacillus species were isolated from urine and vaginal swabs (HVS), respectively....

Author(s): Ifeoma M. Ezeonu and Jennifer U. Kanu

In vitro antagonistic activity evaluation of some selected fungi isolated from burned soils in Mila region (East of Algeria)

July 2016

The present study was initiated to (i) determine burned forest-inhabiting fungi in Zouagha, TerriBeinène, Mila and (ii) study the antagonistic activities of Trichoderma sp against Fusarium sp, Penicillium sp, Rhizoctonia sp, and Alternaria sp. Eighteen fungal strains representing six genera were isolated from soil samples obtained from the burned forest of Zouagha in the Mila region: Trichoderma sp, Fusarium sp,...

Author(s): Ouidad Abdelaziz, Fayza Kouadri, Naouel Khiat and Insaf Khiat

Detection of virulence and antibiotic resistance genes in environmental strains of Vibrio spp. from mussels along the coast of Rio de Janeiro State, Brazil

June 2016

Mussels have a filter system enabling them to take up nutrients from the water, so a microbiological analysis of these bivalve mollusks can show the contamination levels of their surrounding aquatic environment. The present work aimed to isolate Vibrio species from two hundred samples of mussels (Perna perna) incrusted on rocks of the Santana Archipelago and from longline mariculture in Ilha Grande Bay in Angra dos...

Author(s): Marcelo Santos de Oliva, Greiciane França Bronzato, Lidiane de Castro Soares, Ingrid Annes Pereira, Bruno Rocha Pribul, Marco Antônio Soares de Souza, Shana Mattos de Oliveira Coelho, Irene da Silva Coelho, Dália dos Prazeres Rodrigues and Miliane Moreira Soares de Souza

Reconstructed H3N2 influenza virus predicted from influenza vaccine strains improved cross-protective immunity in mice

June 2016

Hemagglutinin (HA) protein of influenza virus is a core antigen protein which induces protective antibody in hosts. But HA genes mutate rapidly. Every year, World Health Organization (WHO) selects representative influenza virus strains from the influenza centers worldwide for virus vaccine production. So, the selected influenza vaccine strains, can partly respond to antigen drifts of circulating influenza virus,...

Author(s): Fangye Zhou, Jinshui Li, Hongwei Ning, Jing Tan, Yupeng Xiao and Qinqin Chen

Reduction of organic contaminants and microbial communities in bioecological wastewater treatment system

June 2016

Wastewater treatment is a key factor in controlling eutrophication of freshwater bodies. Nutrients discharge in wastewater can cause water quality problems such as eutrophication of freshwater bodies, decreased conservation and recreational value of water systems, and destruction of aquatic life. A pilot scale bioecological wastewater treatment system which consisted of a modified anaerobic\anoxic\oxic (A2O) system as a...

Author(s): Haq Nawaz Abbasi, Xiwu Lu and Khawar Ali Shahzad

Serotyping and molecular typing of Salmonella species isolated from wastewater in Nsukka, Nigeria

June 2016

The objective of this investigation was to isolate and identify Salmonella serovars present in wastewater from the University of Nigeria, Nsukka (UNN) wastewater treatment plant and to evaluate the sensitivity and precision of different microbial typing methods (conventional and molecular), in identifying and characterizing Salmonella species. A total of 100 suspected Salmonella colonies on selective media...

Author(s): Dickson Ihenrochi Dickson, Ike Anthony Chibuogwu and Ezeonu Ifeoma Maureen

Nutritional characteristics of marandu grass (Brachiaria brizantha cv. marandu) subjected to inoculation with associative diazotrophic bacteria

June 2016

Due to the search for viable and cost-effective ways to provide nitrogen for forage grasses, inoculation with diazotrophs presents high potential as an alternative to plant nutrition by reducing the use of nitrogen fertilizers. The objective of this work was to evaluate the nutrition of marandu grass (Brachiaria brizantha cv. marandu) subjected to inoculation with associative diazotrophic bacteria grown in Cerrado soil...

Author(s): Salomao Lima Guimaraes, Carolina Silva Alves dos Santos, Edna Maria Bonfim-Silva, Analy Castilho Polizel and Eder Rodrigues Batista

Screening and evaluation of herbicidal metabolites produced by Trichoderma spp.

June 2016

Laboratory bioassays were conducted to screen and evaluate the potential herbicidal metabolites produced by Trichoderma.longibrachiatum (Tr673), T. harzianum (Tr319), T. viride (Tr347) T. koningii (Tr324) and T. asperellum (Tr85) on inhibiting seed germination (SGe) and shoot/root growth (SGr/RGr) of seedlings of Porslane (Portulaca oleracea), Barnyardgrass (Echinochloa crus-galli), and Amaranthus retroflexus L. The...

Author(s): Wenfeng Kuang, Chengfan Wang and Weili Mao

Antibiotic susceptibility patterns of Pseudomonas aeruginosa isolated from clinical and hospital environmental samples in Nasiriyah, Iraq

June 2016

A total of one hundred and forty two swab samples (92 clinical and 50 from hospital environment) were collected for the detection of Pseudomonas aeruginosa. Out of the total samples, 29 isolates of P. aeruginosa were isolated and recorded an overall prevalence rate of 20.42% (29/242) of which 18 (19.56%) were from wounds and burns swabs of patients, and 11 (22%) were from hospital environment. The highest rate of P....

Author(s): Jawad R. Al-Zaidi

Antibacterial activity of silver-nanoparticles against Staphylococcus aureus

June 2016

In this study, the antibacterial activity of silver nanoparticles (Ag-NPs) as Ag-nano disc was assessed with respect to Gram positive bacteria. Scanning electron microscope (ESM) was used for Staphylococcus aureus by measuring the diameter of inhibition zones in culture media and Ag-NPS. Eventually, gauzes containing strain were further impregnated between 2-layer nanofilms at 1, 3, 5 and 7 ppm sized 2 × 2 cm and...

Author(s): Mahsa Ydollahi, Hamed Ahari and Amir Ali Anvar

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

June 2016

Concern with traces of antibiotics found in milk and resistant bacteria has led to the search for alternatives, mainly natural products, to treat mastitis. This study aimed at evaluating the antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from mastitic milk and at analyzing their fatty acids. The following microorganisms were isolated from milk produced by...

Author(s): Cristina Hallal de Freitas, Carolina Lambrecht Goncalves, Josiara Furtado Mendes, Deborah Murowaniecki Otero, Claudio Martin Pereira de Pereira, Helenice de Lima Gonzales and Patricia da Silva Nascente

Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat

June 2016

Seroepidemiology, molecular and entomological studies of bluetongue in sheep was carried out in Gujarat, India. Out of 980 sera sample screened, 361 (36.84 %) were found positive by c-enzyme-linked immunosorbent assay (c-ELISA). Out of the 103 blood samples tested for the presence of bluetongue virus (BTV) by reverse transcription-polymerase chain reaction (RT-PCR), 3 were found positive, producing 274 bp amplicons with...

Author(s): Chauhan H. C., Bhagat A. G. , Dadawala A. I. , Patel B. K. , Chandel B. S. , Kher H. N. , Patel M. A. and Rajgor M.

Diversity of MRSA SCCmec elements in Pretoria region of South Africa: The problem of variation in the assigned SCCmec types by different multiplex-polymerase chain reaction (PCR) methods and a call for an African consensus

June 2016

The SCCmec element is one of the recommended targets for MRSA characterization and several multiplex-PCR SCCmec typing methods have been developed over the past years. However, there are no data on the consistency of the SCCmec types in clinical isolates as detected by these methods. Using different previously published, commonly used M-PCR methods, this report describes the diversity of SCCmec elements in MRSA isolates...

Author(s): John F. Antiabong, Marleen M. Kock, Tsidiso G Maphanga, Adeola M. Salawu and Marthie M. Ehlers,

Systemic elicitation of defense related enzymes suppressing Fusarium wilt in mulberry (Morus spp.)

June 2016

Antagonist and organic amendments based bioconsortia (seri bed waste+Pf1+Bs4+Th1+neem cake) was found to lead the restriction of Fusarium solani pathogen in mulberry roots mainly by inducing inherent defense enzymes. Induction of defense enzymes such as peroxidase, polyphenoloxidase, phenylalanine ammonia lyase, phenols, catalase and superoxide dismutase, was studied in mulberry plants pretreated with bioconsortia and...

Author(s): NARAYANAN Palani, PARTHASARATHY Seethapathy, RAJALAKSHMI Jeyaraman, ARUNKUMAR Kathaperumal and VANITHA Shanmugam

Special biochemical profiles of Escherichia coli strains isolated from humans and camels by the VITEK 2 automated system in Al-Ahsa, Saudi Arabia

June 2016

The VITEK 2 automated system was used for comparing biochemical characteristics of Escherichia coli isolates from human urinary tract infections (UTIs) and camel faecal samples for the first time in the study area. Identification by the system to the species level was accurate. Recovery rate of E. coli from camel specimens was 26% and for human specimens was 33%. Based on biochemical activities, human and animal strains...

Author(s): Naser A. Al Humam

Microbial safety criteria and quality of traditional Egyptian Karish cheese

June 2016

Some traditional fermented dairy products like Egyptian Karish cheese and traditional fermented milk (Laban Zeer) are manufactured in farmhouses following traditional techniques using raw milk without heat treatment or addition of selected starter cultures, these products are generally designed as “artisanal”. The representative Karish samples were analyzed for their content of total microbial,...

Author(s): Sameh Awad

Outbreak of Burkholderia cepacia complex associated with contaminated liquid soap for hospital use: A case study

June 2016

Burkholderia cepacia complex (BCC) is gram negative bacteria which causes different types of human infections including catheter-associated infections and respiratory tract infections especially in immunocompromised  patients. This study was conducted to decide the source, to prevent the possible hospital outbreak of BCC and to determine the epidemiological source. Eleven cartridge of liquid soaps were purchased by...

Author(s): Kaya Süer, Meryem Güvenir , Barış Otlu and Emine Tunç

Antibacterial activity Lactobacillus plantarum isolated from fermented vegetables and investigation of the plantaricin genes

June 2016

Escherichia coli and Staphylococcus aureus are two food pathogens that cause severe food poisonings. Another problem found on a global level is the continuous increase of antimicrobial resistance in bacteria isolated from food. This study aimed to evaluate the antibacterial activity of Lactobacillus plantarum against pathogenic bacteria including E. coli and S. aureus and to study if L. plantarum with antibacterial...

Author(s): Min-Hsiu Chang, Shu-Feng Hong, Jiau-Hua Chen, Mei-Fang Lin, Chin-Shuh Chen and Shu-Chen Wang

Anti-HSV type-1 activity of olive leaves extract crude form acting as a microemulsion dosage form

June 2016

Olea europeae (L.) has been reported to have antibacterial, antifungal and antiviral activities. The aim of the present study is to evaluate antiviral activity of olive leaves extract (OLE) against herpes simplex viruses (HSV) type-1 virus. Screening of antiviral activity was assessed by measuring inhibition of viral-induced cytopathic effect of in vero cells of different OLE fractions that have been successively...

Author(s): Rania Abdelmonem Khattab, Alaa El-Din Shawky Hosny, Mostafa Ali Abdelkawy, Rania Hassan Fahmy and Nariman Alaa ElMenoufy,

Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections

June 2016

Many strains of Klebsiella pneumoniae can produce several virulence factors, such as siderophores, capsules, fimbrial adhesins and antibiotic resistance enzymes, which are important in the adherence, colonization, invasion and development of the infection. Therefore the aim of this study was to investigate ten virulence gens in 32 K. pneumoniae isolated from different clinical infections: urine (13), burns (12), sputum...

Author(s): Ahmed Abduljabbar Jaloob Aljanaby and Alaa Hassan Abdulhusain Alhasani

Diclofenac inhibits virulence of Proteus mirabilis isolated from diabetic foot ulcer

June 2016

Diclofenac is an analgesic and anti-inflammatory drug, used to relief the secondary complications of diabetes. Wound infections are much more serious particularly in diabetic patients. Proteus mirabilis are Gram negative rods, show a wide range of pathogenesis based on arsenal of diverse virulence factors. Recently, P. mirabilis and other Gram negative rods were isolated from diabetic foot ulcers; moreover, these...

Author(s): Wael Abdel Halim Hegazy,

Hydrophobicity and specific biofilm features of Bacillus cereus spores subjected to pH stresses

June 2016

Bacillus cereus is a foodborne pathogen that often persists on food processing surfaces due the formation of spores and biofilms. Spores of 12 selected B. cereus strains from genotypes that recurred in a pasteurized milk processing line were investigated in this study, for their surface and biofilm characteristics. The main objective was to have an insight into their persistence strategies. Spore surface hydrophobicity...

Author(s): Fadila Malek

Phylogenetic analysis of Campylobacter jejuni from human and birds sources in Iraq

June 2016

The present study was designed for a phylogenetic tree analysis of Campylobacter species as molecular method for genetic identification of Campylobacter jejuni from human and birds sources and birds and amplified by polymerase chain reaction assay using specific primers for 16S rRNA gene of Campylobacter jejuni (GenBank: EF136575.1). In this study, the multiple sequence alignment analysis and neighbor joining...

Author(s): Huda Abdal-Hadei Ali Al-Nasrawi

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

June 2016

Soybean (Glycine max L.), the most popular oil seed crop, grown in the experimental fields of Tamil Nadu Agricultural University and adjoining areas exhibited symptoms of necrosis and stunting. The symptoms were similar to bud blight of soybean caused by the Tobacco streak virus (TSV) and the disease is most prevalent throughout the country. To study the nature of infection, sap inoculation of the soybean strain induced...

Author(s): Rajamanickam, S., Ganesamurthy, K. and Karthikeyan, G.

Biofixation of CO2 on a pilot scale: Scaling of the process for industrial application

June 2016

The use of Spirulina in CO2 biofixation, aside from its contribution to reducing the greenhouse effect, enables the use of the biomass to obtain biocompounds. In this work, Spirulina platensis was used for CO2 biofixation under different conditions of inorganic carbon. S. platensis was inoculated into 200 L bioreactors containing modified Zarrouk’s medium (concentration of the carbon was from 1.0 to 2.0 g.L-1)....

Author(s): Felipe Camerini, Michele da Rosa Andrade Zimmermann de Souza, Michele Greque de Morais, Bruna da Silva Vaz, Etiele Greque de Morais and Jorge Alberto Vieira Costa

Antibacterial activity of extracts of three aromatic plants from Burkina Faso against rice pathogen, Xanthomanas oryzae

May 2016

Plant extracts can have powerful antibacterial activity and may be used as biological control against important plant pathogens. This study assessed effect of extracts of Cymbopogon citratus, Eucalyptus camaldulensis and Mentha piperita against two pathovars of Xanthomonas oryzae attacking rice. Both in vitro and in vivo approaches were used for different strains of X. oryzae originating from Burkina Faso. The three...

Author(s): Wonni I., Ouedraogo S. L., Ouedraogo I. and Sanogo L.

Foliar application of Azospirillum brasilense in soybean and seed physiological quality

May 2016

Bacteria of the Azospirillum genus have considerable potential for application in agricultural systems, either in co-inoculation and foliar application to increase crop yields, due to its role in the production of phytohormones. The objective of this research was to evaluate the yield and quality of soybean seeds produced under the effect of different doses of Azospirillum brasilense bacteria applied to the leaves....

Author(s): Alan Mario Zuffo, Adriano Teodoro Bruzi, Pedro Milanez de Rezende, Maria Laene Moreira de Carvalho, Everton Vinicius Zambiazzi, Igor Oliveri Soares and Karina Barroso Silva

Association study of single nucleotide polymorphism of human Toll like receptor 9 and susceptibility to pulmonary tuberculosis in Egyptian population

May 2016

Toll-like receptors (TLRs) are known to play important roles in human innate immune systems. Polymorphisms in and functions of TLRs have been investigated to identify associations with specific infectious diseases including tuberculosis (TB). This study was performed for 166 samples of unrelated individual's diagnosis of pulmonary tuberculosis and 98 household healthy samples. Genomic DNA was extracted from...

Author(s): Samia M. Omran, Zeinat K. Mohamed, Zainab Zakaria, Yasser M. Abd-Elmonem and Khaled E. El Gayar,

Bacteriological and molecular studies on Clostridium perfringens isolated from sheep in three Egyptian provinces

May 2016

The study was intended to determine types of Clostridium perfringens and their toxins in diseased sheep with suspected enterotoxaemia, apparently healthy in contact sheep and soil in three Egyptian provinces (one year study). A total of 800 sheep were visited regularly over a period of one year to record cases of enterotoxaemia and collection of samples for bacteriological examination and toxin genotyping using...

Author(s): Elsify A., Tarabess R., Nayel M. A., Salama A., Allaam M., Abd El-Gaber M., Hassan H., Zaghawa A. and Elballal S.

Effect of cholesterol lowering multiplex lactic acid bacteria on lipid metabolism in a hamster model

May 2016

This study aimed to investigate the effects of a probiotic complex product including Pediococcus, Lactobacillus, and Bifidobacteria on the lipid metabolism of hamsters fed a high-fat and high-cholesterol diet. Fifty male Syrian hamsters were assigned to five experimental groups: control, high-fat plus high-cholesterol diet (HFC), and HFC supplemented with low-, medium-, and high-dose of probiotic product. The hamsters...

Author(s): Cheng-Chih Tsai, Lan-Chun Chou, Shu-E Lai and Chung-Chih Huang

Effect of non-steroidal anti-inflammatory drugs and dexamethazone on the biofilm formation and expression of some adhesion-related genes of Candida albicans and Staphylococcus aureus

May 2016

Candida albicans and Staphylococcus aureus can cause many diseases which are considered in many cases life threatening infections. Their biofilm formation on the surface of medical devices is difficult to be treated. Our study evaluates the effect of some anti-inflammatory drugs on the growth, biofilm formation and the expression of some adhesion-related genes. Antimicrobial activity of the tested drugs was determined...

Author(s): Rehab Mahmoud Abd El-Baky and Sherein G. El-Gendy

Effect of tyrosol on Staphylococcus aureus antimicrobial susceptibility, biofilm formation and virulence factors

May 2016

Staphylococcus aureus is considered the most common causative agent of community and hospital-acquired infections. The isolates with increased virulence and antimicrobial resistance, especially mecithillin resistant S. aureus (MRSA), represent a challenge in hospitals and health care facilities worldwide. The effect of tyrosol, a phenolic quorum sensing compound of Candida albicans, was studied on the sensitivity of...

Author(s): Shaymaa H. Abdel-Rhman and Dina E. Rizk

Removal capacity of faecal pathogens from wastewater by four wetland vegetation: Typha latifolia, Cyperus papyrus, Cyperus alternifolius and Phragmites australis

May 2016

The ability of four wetland vegetation: Typha latifolia, Cyperus papyrus, Cyperus alternifolius and Phragmites mauritianus in removing pathogenic and indicator microorganisms in the wetlands were studied in bucket experiments. The findings suggested that vegetated systems can effectively reduce faecal pathogens in wastewater. Both Salmonella species and Escherichia coli removal efficiencies were above 98%. This proved...

Author(s): H. J. Kipasika, J. Buza , W. A. Smith and K. N. Njau

Penicillium citrinum VFI-51 as biocontrol agent to control charcoal rot of sorghum (Sorghum bicolor (L.) Moench)

May 2016

In our earlier investigation, a fungal isolate Penicillium citrinum VFI-51 and its secondary metabolite was reported to have antagonistic potential against Botrytis cinerea, the causative agent of Botrytis gray mold disease in chickpea. In the present investigation, P. citrinum VFI-51 was further evaluated for its antagonistic potential against Macrophomina phaseolina, the causative agent of charcoal rot in sorghum. P....

Author(s): Meesala Sreevidya, and Subramaniam Gopalakrishnan

Effects of growth-promoting endophytic Methylobacterium on development of Citrus rootstocks

May 2016

Endophytic Methylobacterium spp. were inoculated on citrus seed and evaluated for their ability to promote growth of Citrus limonia and Citrus sunki seedlings under commercial nursery conditions. The germination rate and seedlings growth differed according to the combination between Methylobacterium species and citrus rootstock, showing that the interaction depends on their compatibility. Methylobacterium had no effect...

Author(s): Andréa Cristina Bogas, Carlos Ivan Aguilar-Vildoso, Aline Aparecida Camargo-Neves and Welington Luiz Araújo

Molecular characterization and virulence of Beauveria bassiana and Metarhizium anisopliae against Galleria mellonella (Lepidoptera: Pyralidae) and Tenebrio molitor (Coleoptera: Tenebrionidae) larvae

May 2016

Entomopathogenic fungi considerably vary in their action and virulence mode. The contamination mainly depends on the adhesion and penetration of the fungus in the host integument. Four isolates from Beauveria bassiana and Metarhizium anisopliae were molecularly characterized by rDNA-ITS sequencing. Their virulence against last instar larvae of Galleria mellonella and Tenebrio molitor at four conidia concentrations:...

Author(s): Margy A. Esparza Mora, Julie G. Chacón-Orozco, Ricardo Harakava, Janaina Ribeiro Costa Rouws and Marcelo E. Fraga

Nodular bacterial endophyte diversity associated with native Acacia spp. in desert region of Algeria

May 2016

Five species of Acacia (Acacia ehrenbergiana Hayne, A. nilotica (L.) Delile, A. seyal Delile, A. tortilis (Forssk.) Hayne and A. laeta Delile) indigenous to Tamanrasset (Algeria) were investigated for their nodulation status and nodular endophytic diversity. A. ehrenbergiana showed the highest nodulation ability across the different sites in this region, indicating the widespread occurrence of compatible rhizobia in the...

Author(s): Zineb Faiza Boukhatem, Chahinez Merabet, Abdelkader Bekki, Sonia Sekkour, Odile Domergue, Robin Dupponois and Antoine Galiana

Incidence of zoonotic Campylobacter jejuni in fast meal meat, grill chickens and symptomatic Egyptians

May 2016

Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains of C. jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and produces diffuse bloody edematous exudative enteritis. The common routes of transmission are fecal-oral, person-to-person and the eating of raw or undercooked chickens or meat. This study recognizes the incidence of zoonotic...

Author(s): Hassan A. El Fadaly, Ashraf M. A. Barakat, Khaled A. Abd El-Razik, Azza S. M. Abuelnaga and Elgabry A. Elgabry

Efficacy of plasmid curing agent on Streptomyces longsporesflavns

May 2016

Plasmid-specified traits like lactose metabolism and L-asparginase production could be eliminated from Streptomyces longsporesflavns culture during production of fermented product (Microbial succession). In this study we mainly focus on plasmid profiling and plasmid characterization from S. longsporesflavns isolated from marine soil. Then we observe the efficacy of plasmid curing agent on S. longsporesflavns....

Author(s): T. R. Sivashankari, K. Sudha, S. Barathi and V. Karthikeyan

Suppression of duck hepatitis A virus Type 1 replication by lentivirus-mediated RNA-dependent RNA polymerase (RdRp) gene-specific siRNA

May 2016

To explore the application of RNAi technology for the control of duck hepatitis A virus type 1 (DHAV-1). The RNA-dependent RNA polymerase (RdRp) gene was amplified by PCR. pEGFP-RdRp was constructed for fusion expression of EGFP-RdRp protein. According to the sequence of RdRp, three gene-specific siRNAs were designed and the corresponding shRNA was inserted into pmiRZipΔ to construct pRdRp-shRNA. pRdRp-shRNA and...

Author(s): Yong-Juan Wang, Shan-Yuan Zhu, Ping-Fu Cui, An-ping Wang, Wei-Ming Hong, Hui Lu and Wei-Yong Zuo

Studies on leukocidins toxins and antimicrobial resistance in Staphylococcus aureus isolated from various clinical sources

May 2016

Staphylococcus aureus toxins represent a public health challenge all over the world. This study aims to analyze the prevalence of genes encoding the staphylococcal leukocidins and their correlation with antimicrobial susceptibility and the source of isolation. For this purpose, the susceptibility of 75 S. aureus isolates to 12 antimicrobial agents was investigated. The leukocidins genes (lukD, lukE, lukF and lukS) were...

Author(s): Abdel-Halem A. Abdel-hamed, Shaymaa H. Abdel-Rhman and Mohamed A. El-Sokkary

Antimicrobial activity of several Brazilian medicinal plants against phytopathogenic bacteria

May 2016

What is currently raised as a new approach in the management of plant diseases is the development and formulation of plant based biopesticides. The objective of present study is to evaluate the antibacterial activity of aqueous extracts of twelve species belonging to seven families collected from the Northeast of Brazil against four economically important phytopathogenic bacteria. Antibacterial activities of the aqueous...

Author(s): Cibele Maria Alves da Silva, Bruna Mirely da Silva Costa, Alexandre Gomes da Silva, Elineide Barbosa de Souza, Márcia Vanusa da Silva, Maria Tereza dos Santos Correia, da Silva Ana Paula Sant´Anna and Lima Vera Lúcia de Menezes

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