African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5238

AJMR Articles

Production of fermented sweet potato flour using indigenous starter cultures

November 2016

The present study develop various microorganisms for starter culture purpose from fermented sweet potato and used the developed starters in the fermentation of sweet potato and products for consistent quality product with improved hygiene. Sweet potato was purchased from different markets and microorganisms were isolated and developed as starters. Comparative studies on different starters for the fermentation of sweet...

Author(s): Ajayi, O. I., Ehiwuogu-Onyibe, J., Oluwole, O. B., Jegede, A. A., Salami, T. A., Asieba, G. O., Chiedu, I. E., Suberu, Y. L., Aba, E. M., Dike, E. N., Ajuebor F. N. and Elemo, G. N.

Soil enzyme activities and soil microbe population as influenced by long-term fertilizer management during the barley growth in Hunan Province, China

October 2016

The effects of long-term fertilizer management on soil enzyme activities and soil microbe population under double-cropping paddy fields in southern China was studied. The purpose of this study was to explore the changes of soil enzyme activities and soil microbe population as related to mineral fertilizer and manure and rice residue based on a long-term field experiment. The experiment was initiated in 1986 and...

Author(s): Haiming Tang, Xiaoping Xiao, Jimin Sun , Lijun Guo, Ke Wang, Weiyan Li and Wenguang Tang

Effect of gamma irradiation (Co60) in the control of Campylobacter sp. in chilled chicken (Gallus gallus) heart

October 2016

The present study aimed to evaluate the efficiency of the irradiation process in the control of Campylobacter spp. in chilled chicken heart samples, since this microorganism is related to the contamination of meat and chicken giblets and is responsible for enteritis in humans. The methodology and standards recommended by RDC no. 12 (Brazil, 2001) were applied for the bacteriological analyses. The chilled chicken heart...

Author(s): Marta Maria Braga Baptista Soares Xavier, Robson Maia Franco, Ana Beatriz Monteiro Fonseca, Edgar Francisco Oliveira de Jesus, Mauro Carlos Lopes Souza, Sheila da Silva Duque, Wagner Thadeu Cardoso Esteves

Efficacy of biocontrol agents in the management of head rot of cabbage (Brassica oleracea var. capitata) caused by Sclerotinia sclerotiorum

October 2016

Head rot of cabbage caused by Sclerotinia sclerotiorum leads to rotting of fully matured cabbage heads in the field. In the present study the antagonistic effects of twenty Bacillus isolates was tested against S. sclerotiorum in vitro. Eight effective Bacillus isolates obtained from studies in vitro, commercial formulations of Trichoderma viride isolate TV-1 and Pseudomonas fluoroscens isolate Pf-1along with a fungicide...

Author(s): Kamesh Krishnamoorthy K., Sankaralingam A. and Nakkeeran S.

Microbiological and molecular characterization of environmental mycobacterium strains isolated from the Buruli ulcer endemic and non-endemic zones in Côte d’Ivoire

October 2016

Mycobacterium ulcerans (MU), the causative agent of Buruli ulcer (BU), skin disease, is considered to be an environmental pathogen. The pathogenic virulence of MU is being linked to the expression of toxin called Mycolactone. Genetic analyses have shown the high diversity with variable number tandem repeats (VNTR) and mycobacterial interspersed repetitive units (MIRU) in M. ulcerans and in mycolactone producing...

Author(s): Sabine N. Vakou, Julien Coulibaly-Kalpy, Solange E. Kakou-Ngazoa, N`guetta Aka, N`golo D. Coulibaly, Mireille Dosso  and Allico J. Djaman,

External ocular bacterial infections among Sudanese children at Khartoum State, Sudan

October 2016

Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye component can be infected by a diversity of bacteria. The present study was performed to determine the prevalence of external ocular bacterial infections and to find out antibiotic susceptibility pattern of bacterial isolates at eye care hospitals in Khartoum, Sudan. Two hundred and four corneal scrape and drained pus...

Author(s): Mazin O. Mohager, Lemya A. Kaddam and Samah O. Mohager

Pneumonia in Algerian Ouled Djellal sheep: Bacteriological study and macroscopic aspect of lung lesions

October 2016

Respiratory diseases in sheep are a multifactorial etiology syndrome, causing great economic losses. This study was carried out with the aim of establishing a bacteriological diagnosis of respiratory diseases in sheep, on the basis of lungs with macroscopic lesions taken from sheep slaughtered at the Blida abattoir. A total of 150 samples (swabs and lung parenchyma) from 75 Ouled Djellal sheep, aged six to twelve months...

Author(s): Kamel Akloul and Mohammed Nabil Menoueri

Anti-biofilm activity of ibuprofen and diclofenac against some biofilm producing Escherichia coli and Klebsiella pneumoniae uropathogens

October 2016

Ibuprofen (IBU) and Diclofenac (DIC) are non-steroidal anti-inflammatory drug which have shown to have broad antimicrobial activity and limit biofilm formation. Aim of this study was to evaluate the effects of IBU and DIC against biofilm formation strong strains Escherichia coli and Klebsiella pneumoniae associated with urinary tract infections (UTIs). Additionally some virulence factors, antibiotic resistance, ESBL...

Author(s): Rosa Baldiris, Victor Teherán, Ricardo Vivas-Reyes, Alfredo Montes and Octavio Arzuza

Effects of immunoglobulin Y (IgY) serum against plaque bacteria

October 2016

Dental caries is an infectious disease caused by plaque bacteria, especially Streptoccus mutans. Many studies were conducted to develop methods or material for dental caries prevention. It was using passive immunization that will produce specific antibodies. Yolk immunoglobulin (IgY) is antibody in the serum and egg yolk. This study aimed to determine the effect of IgY serum against the growth of dental plaque bacterial...

Author(s): Juni Handajani, Dhinintya Hyta Narissi, Mufidana Azis and Aurita Siwi Rahmawati

Campylobacter in sheep, calves and broiler chickens in the central region of Algeria: Phenotypic and antimicrobial resistance profiles

October 2016

The study was conducted in four slaughterhouses in Bouira Province, central region of Algeria. Campylobacter in the main food animals (sheep, calves and broilers) were studied to evaluate the prevalence, phenotypic characteristics and antibiotic susceptibility of isolated strains. Out of 200 sheep, 200 calves and 100 broilers swab samples collected. 150 strains were isolated and identified. A study of sensitivity to 14...

Author(s): Meryem Guessoum, Zehor Guechi, Fares Aigoun, Sadjia Mahrane and Amina Hachemi

Bacterial association and oral biofilm formation: A bibliometric analysis

October 2016

Biofilms are complex aggregations of microorganisms formed in any situation where there is contact with superfaces solids, liquids or gases. This study aims to map the international scientific literature on the formation, bacterial adhesion, prevention and treatment of oral biofilms. There were 921 published articles in 274 different journals indexed in the Web of Science, in the 1991-2015 year period. These articles...

Author(s): Luana Kelle Batista Moura, Fabrício Ibiapina Tapety, Mitra Mobim, Eliana Campelo Lago, Eduardo Souza de Lobão Veras, Carla Maria de Carvalho Leite Leal Nunes, Tanit Clementino Santos and Thiago Lima Monte

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

October 2016

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and a leading cause of hospital- acquired infections. Characterization of the isolates from different infection sites might help to control infections caused by the pathogen. The aim of the present work is to characterize P. aeruginosa isolates recovered from different clinical specimens at two hospitals in Cairo with regard to their antibiogram,...

Author(s): Marwa M. Raafat, Marwa Ali-Tammam and Amal E. Ali,

Effectiveness of augmented consortia of Bacillus coagulans, Citrobacter koseri and Serratia ficaria in the degradation of diesel polluted soil supplemented with pig dung

October 2016

Laboratory studies were developed to compare the effectiveness of inoculated bacteria consortia and indigenous microorganisms on diesel-polluted soil for 18 days. Bacteria isolated from the unpolluted soil sample were: Pseudomonas spp. (LBI), Pseudomonas cepacia (LB5), Micrococcus luetus (LB2), Bacillus subtilis (LB3) and Bacillus cereus (LB4). Their ability to degrade different substrates were first studied by the...

Author(s): Fagbemi O. Kehinde and Sanusi A. Isaac

Impact of land use and soil types on arbuscular mycorrhizal fungal diversity in tropical soil of India

October 2016

A study was undertaken along land use gradients with different soil types in subtropical ecosystem of Northern India to evaluate the accurance of arbuscular mycorrhizal fungi (AMF). The gradients was from natural land with forest tree, farmer’s field under chemically managed, farmer’s field under organically managed and Industrial wasteland with five different plant species. We investigate the total AMF...

Author(s): Sanjeev  Kumar and Alok Adholeya

Development and evaluation of sundae-type “Coalhada” containing Lactobacillus paracasei and blueberry (Vaccinium ashei) preparation

October 2016

“Coalhada” is a type of fermented milk (made with Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris) very popular in Brazil, which is widely consumed due to its pleasant texture and mild taste and aroma. Its acceptance is possible to increase by adding a fruit preparation. Fermented milk is the most common food matrix used for delivery probiotic bacteria, which are able to survive to the...

Author(s): Maria Eduarda Daussen Dutra, Daiane Aparecida Paggi, Luiza Martins Reguse, Ana Carolina Sampaio Doria Chaves and Deise Helena Baggio Ribeiro

Soil bacteriological pollution in pig farm vicinity: Assessment of bacterial dynamics and detection of antimicrobial resistance gene

October 2016

Emissions of biological contaminants (microbes) from intensive pig farming may cause environmental problems due to lack of proper waste management. This work was conducted to assess bacteriological pollution of soil in pig farm and to detect the presence of antibiotic resistance gene of the prevailing bacteria. Soil samples were collected from March to August 2013. The method included bacterial enumeration (10 -1 to 10...

Author(s): Dikonketso Shirley-may Matjuda and Olayinka Ayobami Aiyegoro

Assessment of the microbial quality of some oral liquid herbal medicines marketed in Ile-Ife, South-western Nigeria

October 2016

The extensive use of herbal medicinal products in the treatment and management of disease states within communities in Ile-Ife, south western Nigeria has made it imperative to investigate the microbial quality of a sample of these products in the light of the standards prescribed by regulatory bodies. This study was therefore carried out to assess the microbial quality of locally prepared and unregistered herbal oral...

Author(s): Oluwatoyin Abimbola Igbeneghu and Adebayo Lamikanra

Modification of pathogenic microbiota and histology of gastrointestinal tract of Archachatina marginata (Swainson, 1821) by Carica papaya seed meal

October 2016

This study investigated the bactericidal efficacy of the ethanolic extract of Carica papaya seed on snail gastrointestinal tract (GIT) microbiota and associated histological changes. The bacterial isolates were characterized based on colony morphology, culture characteristics and biochemical tests. Each portion of the gut was further subjected to histological examination to ascertain the effect of this extract on the...

Author(s): Odo G. E, Ekeh F. N, Ekechukwu, N. E, Agwu, E. J, Aguzie I. O. E. and Ugwuezumba B.

Prevalence and antimicrobial susceptibility profile of Salmonella species from ready-to-eat foods from catering establishments in Jigjiga City, Ethiopia

October 2016

Food-borne pathogens are the leading cause of morbidity and mortality in developing countries. Changes in eating habits, mass catering, unsafe food storage conditions and poor hygiene practices are major contributing factors to food associated illnesses. In Ethiopia, the widespread habit of ready-to-eat food consumption is potential cause of food borne illnesses. The present study aimed at investigating the prevalence...

Author(s): Tesfaye Wolde, Melese Abate, Henok Sileshi and Yohannis Mekonnen

Production and characteristics of bioflocculant TPT-1 from a consortium of Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2

October 2016

The combination of microorganisms in consortia enhances high bioflocculant yields. The study aimed at producing and characterising bioflocculant from Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2. Bioflocculant TPT-1 was obtained through ethanol extraction after optimum conditions were established. The characteristics of the bioflocculant TPT-1 were obtained by scanning electron microscope equipped with...

Author(s): Tsolanku Sidney Maliehe, Nelson Tlou Selepe, Golden Ntombela,  Jean Simonis, Albertus Kotze Basson, Siyanda Ngema, Petunia Samukelisiwe Xaba and Fanelesibonge Mpanza

Occurrence and susceptibility patterns of Campylobacter isolated from environmental water sources

October 2016

Environmental waters are established sources of Campylobacter infections in humans. The aim of this study was to assess the distribution and susceptibility profiles of Campylobacter species isolated from irrigation and domestic water sources in Ghana. Samples were pre-enriched with CCDA broth and isolated on mCCDA agar. Isolates were confirmed on API CAMPY kit and with the Kirby-Bauer disk diffusion method to determine...

Author(s): Akosua B. Karikari, Kwasi Obiri-Danso, Enoch H. Frimpong and Karen A. Krogfelt

Fungi toxic efficiency of some plant volatile essential oils against plant pathogenic fungi

October 2016

Plant essential oils have the potential to replace the synthetic fungicides in the management of different fungal diseases. Four different essential oils of eucalyptus (Eucalyptus globulus), citronella (Cymbopogon citrate), karanj (Pongamia pinnata) and neem (Azadirachta indica) were selected because of their high inhibitory activities against ten phytopathogenic fungi. The antifungal screening for all the four Eos...

Author(s): Shaon Kumar Das, Aniruddha Roy and Harikamal Barman

Bacteria associated with sugarcane in Northeastern Brazil

October 2016

Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select...

Author(s): Michelangelo de Oliveira Silva, Fernando José Freire, Júlia Kuklinsky-Sobral, Emídio Cantídio Almeida de Oliveira, Maria Betânia Galvão dos Santos Freire and Valéria Xavier de Oliveira Apolinário

Effect of temperature, pH and substrate composition on production of lipopeptides by Bacillus amyloliquefaciens 629

September 2016

The lipopeptides surfactin, fengycin and iturin produced by Bacillus species have diverse ecological roles, including antimicrobial activity, induced systemic resistance in plants against pathogens and plant colonization by the producing strain. The conditions that govern both lipopeptide production and plant colonization are not fully understood. The present study investigated the role of growth media, temperature and...

Author(s): Fernando Pereira Monteiro, Flávio Henrique Vasconcelos de Medeiros, Marc Ongena, Laurent Franzil, Paulo Estevão de Souza and Jorge Teodoro de Souza

Screening endophytic actinobacteria with potential antifungal activity against Bipolaris sorokiniana and growth promotion of wheat seedlings

September 2016

Actinobacteria secrete substances that limit or inhibit the growth of plant pathogenic fungi and may be used in the biocontrol of these microorganisms. The aim of this study was to characterize physiological and enzymatic activity of endophytic actinobacteria, evaluate their antifungal activity against Bipolaris sorokiniana root colonization, and evaluate their efficiency in promoting the growth of wheat seedlings....

Author(s): Elisandra Minotto, Luciana Pasqualini Milagre, Cristina Spadari, Thaisa Feltrin, Ana Elisa Ballarini, José Carlos Germani, Sabrina Pinto Salamoni and Sueli Teresinha Van Der Sand

Isolation, screening and statistical optimizing of L-methioninase production by Chaetomium globosum

September 2016

Egyptian soil sample was screened for isolation methioninlytic fungi by a rapid plate assay procedure. Eighteen strains of different isolated fungi were screened quantitavely for their L-methioninase activity. Chaetomium globosum was the most efficacious isolate and a dematiaceous filamentous fungi, it was identified at the molecular level by ribotyping 18S rRNA along with the biochemical characterization, and...

Author(s): Shimaa R. Hamed, Mostafa M. Abo Elsoud, Manal G. Mahmoud, Mohsen M. S. Asker

Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda

September 2016

Increased fish mortality due to infections has forced most farmers to resort the use of chemotherapeutic agents especially antibiotics. The continued use of these drugs in aquaculture is becoming limited as pathogens develop resistance and infer unpredicted long term public health effects. More research efforts are building to identify alternative disease prevention methods, among which the use of probiotics has been...

Author(s): Charles Drago Kato, , Ruth Kabarozi, Samuel Majalija, Andrew Tamale, Nathan Lubowa Musisi and Asuman Sengooba

Rapid microbiological tests for prospecting new fungal strains with high potentiality for the pectinolytic enzymes production

September 2016

Prospecting of new isolates producers of pectinolitic enzymes were performed with cultivation of substrates of rice, soybean and corn bran. Pectinolitic enzymes were observed in cultures of the isolates NFC 1, NFC 2, NFC 4, NFC 5 and NFC 6 identified as belonging to the genus Aspergillus sp. Isolates NFR 1, NFR 2 and NFS1 were identified as belonging to the genus Rhizopus and NFC 3 belonging to the genus Cladosporium;...

Author(s): Cangussu, A. S. R., Felix, M. K. C., Araujo, S. C., Viana, K. F., Aguiar, R. W. S., Ribeiro, B. M., Portella, A. C. F., Barbosa, L. C. B., Brandi, I. V. and Cangussu, E. W. S.

Halophile isolation to produce halophilic protease, protease production and testing crude protease as a detergent ingredient

September 2016

Halophilic enzymes are potentially useful in many industries, particularly in food fermentation, pharmaceutical, textile, and leather for the treatment of saline and hypersaline wastewaters. In this study, a halophilic bacterium was isolated from saltpan environment, and was identified as Bacillus sp. Mk22 through biochemical test and 16S rRNA gene sequencing. During protease screening, the isolates produced 24 mm clear...

Author(s): Ashokkumar Sekar, Mayavu Packyam and Keun Kim

Microbial community structure and chemical composition from dark earth in a native archaeological site of the lower Amazon

September 2016

The microbial community structure from dark earth in a native archaeological site of the Lower Amazon was analyzed by PCR-DGGE, using 16S rRNA gene for prokaryote population and 18S rDNA and ITS regions (using clamp GC) for the eukaryote population. The bands were excised from gel and re-amplified for sequencing. The diversity found according to the region of amplification showed same profiles for the two primers pairs....

Author(s): Maxwel Adriano Abegg, Karina Teixeira Magalhães-Guedes,  Andréia Oliveira Santos and Rosane Freitas Schwan

Prevalence and molecular typing of extended-spectrum β-lactamases in Escherichia coli, Enterobacter cloacae and Citrobacter freundii isolates from Laghouat Hospital, Algeria

September 2016

The antibiotic resistance of enterobacteriacae knows a worldwide worrying evolution with an increase of the extended-spectrum β-lactamases. The present study was to determine the prevalence and molecular typing of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli, Enterobacter cloacae, and Citrobacter freundii, isolated between January 2010 and December 2012, at the Laghouat...

Author(s): Nouria Lagha, Hafida Hassaine, Fréderic Robin, Richard Bonnet and Djamel-Eddine Abdelouahid

Performance of dried blood spot (DBS) PUNCHER and dried blood spots to measure HIV-1 viral load

September 2016

The use of plasma is an obstacle to realize HIV-1 viral load in sub-Saharan Africa. In this context, the dried blood spot (DBS) is an interesting tool for sample collections. This approach was tested using a DBS hole-punch device (PUNCHER). Plasma and DBS samples were obtained from 102 patients, comprising 17 HIV-1 negative patients and 85 HIV-1 infected patients. The PUNCHER’s performance used to cut DBS was...

Author(s): Anoumou Dagnra, Mounerou Salou, Amivi Ehlan, Ahouefa Konou, Alassane Ouro-Medeli, Sika Dossim and Komlan Ali-Edje

Prevalence and antibiotic susceptibility of Staphylococcus aureus from lactating cow’s milk in Bahir Dar dairy farms

September 2016

Staphylococcus aureus is competitive in milk and dairy environments; pathogenic strains have been found to cause disease in their host throughout the world. Therefore, this study was designed to assess the prevalence of Staphylococcus aureus and determine their antibiotic susceptibility from lactating cow milk. A cross-sectional study was conducted in Bahir Dar dairy farms from October 2012 to March 2013. A total of 218...

Author(s): Sileshi Shiferaw and Munees Ahmad

Histamine and microbiological changes during storage of semi-preserved anchovies

September 2016

During the various stages of manufacture of semi-preserved anchovies, histamine shows a significant increase favored by the fragility of anchovies and wealth histidine, an amino acid precursor of histamine. The decarboxylation of histidine is present as a major problem in the industry of semi-preserved anchovies, especially during the ripening step. The objective of this work is to monitor the levels of alteration in a...

Author(s): El Filali F., Hanoune S., Moukrim A., Kaaya A. and Bou’mhandi N.

Molecular genotypes of Mycobacterium tuberculosis strains circulating in Dakar, Senegal

September 2016

Tuberculosis is a contagious infectious disease, in which epidemiologic monitoring by molecular approaches is a critical element of prevention and control. The population structure of the Mycobacterium tuberculosis complex (MTBc) in Senegal was last described in the 1970’s using biochemical methods. In this present study, we applied molecular approaches to genotype M. tuberculosis isolates from active pulmonary...

Author(s): Awa Ba Diallo, Ossaga Gedeon Walbang, Makhtar Camara, Seynabou Lo,  Abigail Ayorinde, Aliou Niang, Souleymane Mboup, Aissatou Gaye Diallo, Bouke Catherine de Jong, and Florian Gehre,

Some parasitic copepods of selected Teleost and Chondrichthyan fishes from the Tunisian gulfs

September 2016

The examination of 415 specimen belonging to twelve species of Teleostean fish and 339 specimen belonging to seven species of Chondrichthyan fish collected from the Tunisian coasts, it enabled us to identify 10 species of parasitic copepods: Lernaeopoda galei, Lernaeocera lusci, Neobrachiella merluccii, Hatschekia mulli, Caligus elongatus, Caligus pageti, Neobrachiella mugilis, Clavellotis fallax, Clavelissa pagri and...

Author(s): Feriel Youssef, Bouchra Benmansour, Oum Kalthoum Ben Hassine and Sabiha Zouari Tlig

Clinico-mycological profiles of dermatophytosis in Jaipur, India

September 2016

Dermatophytosis poses a serious crisis to the socio-economically backward population. The infections are caused by three anamorphic genera; Epidermophyton, Trichophyton and Microsporum which are distributed around the world. The objective of this study was to find out the occurrence, distribution and prevalence of dermatophytes causing human dermatomycosis in various categories of patients in Jaipur (Rajasthan), India....

Author(s): Sachin Kumar and Seema Bhadauria

An in vitro evaluation of Cinnamon (Cinnamomum spp.) and Siwak (Salvadora persica) extracts for controlling the foulbrood pathogens of honeybee

September 2016

Instead of the synthetic antibiotics, the antibacterial activity of the aqueous and ethanolic extracts of both Cinnamon (Cinnamomum spp.) and Siwak (Salvadora persica) plants was evaluated against the foulbrood bacteria under the laboratory conditions. To the study knowledge, it is the first time to evaluate Siwak plant against the foulbrood diseases. The major antimicrobial constituents (total phenolics, flavonoids and...

Author(s): Hashish, Mohamed Elsayied, Khattaby, Ahmed Mahmoud, Khattab, Metwally Mostafa, Gaaboub, Ibrahim Abdallah and Omar, Reda Elsayied

Importance of biofilm in medical sciences: With special reference to uropathogens

September 2016

Biofilm formation is a well organised, genetically-driven process, well characterized for numerous bacterial species which plays important role in urinary tract infections (UTIs). Adherence is a key event initiating each step in UTI pathogenesis. Such UTIs are difficult to treat owing to increased drug resistance within the biofilm cells. The review is mainly focused on biofilm-growing microorganisms because this form...

Author(s): Ghanwate Niraj A., Tiwari Anjali A. and Thakare P. V.

Characterization of antifungal Paenibacillus illinoisensis strain UKCH21 and its chitinolytic properties

September 2016

 Paenibacillus strain, UKCH21, isolated from Uttarakhand State, north western Indian Himalayas was found to produce high levels of extracellular chitinases. The 16s rRNA gene sequence showed 100% homology with P. illinoisensis (Accession No. KR856190) available in the public domain and further phylogenetic study also verified the species identity. The culture supernatants have a maximum chitinase activity of 110.8...

Author(s): Subbanna A. R. N. S., Khan M. S. and Shivashankara H.

Genetic diversity analysis of rep-PCR genomic fingerprinting of Lysobacter spp.

September 2016

Lysobacter. spp. are considered as important biocontrol bacteria, due to their antagonistic activity against many pathogenic fungi, bacteria and nematodes. Rep-PCR was used to analyze the genetic diversity of 12 Lysobacter strains. These strains included Lysobacter antibioticus (HY, 13-1, 13-6, 6-B-1, 13-B-1, 6-T'-4, LJ6-3, LJ6-4 and LR9-3), Lysobacter enzymogenes (DH3, 1-T-1-4) and Lysobacter capsici (LG18) isolate...

Author(s): FU Li-Na, ZHANG Hai-Yan, ZHANG Xiao-Fang, WEI Lan-Fang, ZHANG Jin-Hao and JI Guang-Hai

Evaluation of a real-time PCR assay for the detection of the Klebsiella pneumoniae carbapenemase gene (blaKPC) in enterobacteriaceae isolates from clinical samples in Menoufia University hospitals, Egypt

September 2016

The aim of our work is to study and evaluate a rapid method for detection of Klebsiella pneumonia carbapenemases genes (blaKPC) in enterobacteriaceae isolates from clinical samples by using real time PCR and comparison of this method with ordinary phenotypic methods. Outbreaks of carbapenem‐resistant enterobacteriaceae (CRE), primarily K. pneumoniae, have been reported recently in several regions worldwide. The...

Author(s): Amira Abdelkader Al Hindi and Abeer Hamdy El Shalakany

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

September 2016

Extensive use of antibiotics for urinary tract infections has led to the emergence of drug-resistant microorganisms and one solution to this problem is to search for non-antibiotic compounds that exert anti-bacterial activity through different mechanisms such as non-steroidal anti-inflammatory drugs (NSAIDs). In this study, out of 100 urine samples; 48 Escherichia coli strains were detected, 47.9% were multi-drug...

Author(s): Eman Farouk Ahmed, Rehab Mahmoud Abd El-Baky, Abo Bakr F Ahmed, Nancy Gameel fawzy, Neveen Abdel Aziz and Gamal Fadl Mahmoud Gad

Increasing trend of methicillin-resistant Staphylococcus aureus in Jaipur, Rajasthan, India

September 2016

Multidrug resistant strains of Staphylococcus aureus have become a serious threat to hospitalized patients and health workers. Recently, this bacterium has emerged as methicillin-resistant S. aureus (MRSA) and this bacterial strain has turned into a superbug in the health domain. The main objective of the present study was to find out the prevalence of MRSA in different clinical samples. A total number of 155 clinical...

Author(s): Sachin Kumar and Seema Bhadauria

Comparison of the prevalence of antibiotic-resistant Escherichia coli isolates from commercial-layer and free-range chickens in Arusha district, Tanzania

September 2016

The antibiotic susceptibility of fecal Escherichia coli isolates from commercial-layer and free-range chickens in Arusha district, Tanzania were compared. All the chickens were raised by individual households, but commercial-layer chickens were purchased from commercial vendors, whereas no systematic breeding system was used to produce free-range chickens. A total of 1,800 E. coli isolates (1,200 from commercial-layer...

Author(s): Bernadether T. Rugumisa, Douglas R. Call, Gaspary O. Mwanyika, Murugan Subbiah and Joram Buza

Resistance genes to sulphonamide in commensal Escherichia coli isolated from stool of patients in Mansoura University Children Hospital

September 2016

Commensal bacteria have a great impact on the emergence and spread of antibiotic resistance. This emphasizes a great need to underscore the magnitude of this problem in our locality, and children are taken as a sector in this research because they are usually subjected to heavy load of antibiotic usage. This study aimed at determining sulphonamide resistance genes presence among fecal isolates of commensal Escherichia...

Author(s): Samah Sabry El-Kazzaz, Ghada El-Saeed Mashaly, Amr Mohamed El-Sabbagh and Dina Salama Abd Elmagid

Multidrug-resistant bacteria isolated from patients hospitalized in Intensive Care Unit in University Hospital of Constantine, Algeria (2011 - 2015)

September 2016

The incidence of infections caused by multidrug resistant bacteria is increasing worldwide. The frequent misuse of antibiotic drug has greatly contributed to worldwide dissemination of antibiotics resistance. Multi-drug resistance in Gram-negative and Gram-positive bacteria causes a wide range of infections, particularly in the Intensive Care Unit (ICU) settings leading to an increased impact on morbidity, mortality and...

Author(s): Abla Hecini-Hannachi, Chafia Bentchouala, Abdesselam Lezzar, Houcine Laouar, Kaddour Benlabed, and Farida Smati,

Gut bacterial microbiota in psoriasis: A case control study

September 2016

Gut microbiota is mainly composed of four phyla; however, the human gut microbiota is dominated by only 2 of them and most of them are uncultivable. Psoriasis is an inflammatory skin disorder with associated inflammation of internal organs and musculoskeletal system. This study aimed to, identify numerically abundant bacteria phyla in fecal samples of patients with psoriasis, evaluate whether differences in fecal...

Author(s): Doaa Masallat, Dalia Moemen and Ahmed F. State

Drug-resistant bacteria in frozen and fresh marine shrimp

September 2016

This study aimed to evaluate the bacteriological quality of frozen and fresh shrimp samples by Salmonella detection and the quantification of staphylococci and coliforms. Antimicrobial susceptibility profile of staphylococci isolates was also determined. A total of 30 shrimp samples commercialized in Sobral-CE - 15 fresh and 15 frozen, each one weighing 500 g - was analyzed. There was no contamination by Salmonella...

Author(s): Antonia Jorgiane Rodigues de Macedo, Maria Alana Duarte Marinho, Sara Edwirgens Costa Benício Vasconcelos, and Renata Albuquerque Costa,

Occurrence of Vibrio parahaemolyticus in oysters (Crassostrea gigas) and mussels (Perna perna) of the seacoast of Santa Catarina, Brazil

September 2016

This research aimed to identify and quantify Vibrio parahaemolyticus in fresh oysters (Crassostrea gigas), mussels (Perna perna) and seawater from different regions of cultivation of bivalve shellfishes in the seacoast of Santa Catarina, Brazil. Samples were collected between October 2012 and December 2013 and 130 oysters samples (Crassostrea gigas), 215 mussels samples (Perna perna) and 222 seawater were collected. The...

Author(s): Helen Silvestre da Silva, Karin de Medeiros, Marília Miotto, Clarissa Barreta and Cleide Rosana Werneck Vieira

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