Effects of acidification on the extension of shelf life of Japanese wet-type noodles (woo-long noodle)
December 2016
Noodles are one of the most important foods worldwide; various methods have been reported to extend their shelf life. In this study, the effects of dipping noodles in various concentrations of acidulant solutions (organic acids) on the shelf life of the noodles were evaluated. The results showed that the growth of the tested microorganisms, that is, Escherichia coli, Staphylococcus aureus and Bacillus cereus, was...
Optimization of parameters to increase the xylose reductase production from Candida tropicalis strain LY15 using corn cob as hemicellulose waste substrates
December 2016
Xylose reductase (XR) is a key enzyme in order to obtain xylitol from xylose. It has vast applications in biotechnology including xylitol production. The present study aimed to estimate and characterize XR from Candida tropicalis strain LY15. C. tropicalis strain LY15 showed xylose utilization ability and xylose reductase activity after 48 h of incubation at pH 6.5, incubation temperature 28°C and rotation speed 140...
Rhizobia isolation and selection for serradella (Ornithopus micranthus) in Southern Brazil
December 2016
ability to associate symbiotically with rhizobia. The objective of this study was to isolate, authenticate and select effective rhizobia obtained from native serradella (Ornithopus micranthus) to reduce the need for mineral fertilizer. Nodules, roots of serradella and rhizosphere soil samples were collected in seven municipalities from Rio Grande do Sul and Santa Catarina states. Bacterial colonies were isolated and...
Escherichia coli O157, Staphylococcus aureus and coliforms in crude and processed bovine milk marketed
December 2016
Fifty-five (55) samples from five municipalities in the Recôncavo from Bahia region were analyzed between May and July 2015 to evaluate the sanitary quality and the presence of pathogens of crude and processed bovine milk samples. Psichrotrophic, mesophilic and thermophile rates and the count of Escherichia coli, E. coli O157: H7 Staphylococcus aureus and Listeria monocytogenes were done by microbiological methods...
Molecular characterization of local strains of Bacillus thuringiensis in the North of Algeria
December 2016
The analysis of soil samples taken from orange groves located in four cities of Northern Algeria allowed the isolation and identification of 24 strains belonging to the group Bacillus cereus, based on their physiological, biochemical and molecular characters investigated. They were divided as follow: 12 strains of Bacillus thuringiensis, 1 strain of Bacillus pumilis, 4 strains of Bacillus cereus, 5 strains of Bacillus...
Bacteriocins of Gram-positive bacteria: Features and biotherapeutic approach
December 2016
Bacteriocins are potent antimicrobial peptides produced by every bacterial and archeal species reported to date. The most studied are bacteriocins produced by lactic acid bacteria (LAB) and many species of Bacillus. Knowledge on the classification, biosynthesis and transport of these peptides is changing continually because the discovery and characterization of new bacteriocins increases, thus, the research reports...
Eugenol and linalool: Comparison of their antibacterial and antifungal activities
November 2016
Antimicrobial resistance is one of the greatest threats to human health. Alternatives to antimicrobials are needed to combat the rise of bacterial resistance. Essential oils (EOs) and their components are potential sources of new antimicrobials. The present study was conducted to evaluate the antibacterial and antifungal activities of two components of EOs. The antimicrobial mechanisms of eugenol and linalool were...
Induction of defense mechanisms from filtrates of saprophytic fungi against early blight disease in tomato
November 2016
The induction of defense enzymes presents efficient plant disease management when triggered by metabolic products of microorganisms. The aim of the present study is to select filtered saprobes fungi used for the management of early blight of tomato by inducing pathogenesis related to tomato plant. Filtrates of the fungi Curvularia eragrostidis, Curvularia inaequalis, Memnoniella echinata and Pseudobotrytis terrestris...
Survey of Cryptococcus neoformansin pigeon (Columba livia) excreta in Public Square in Umuarama, Paraná, Brazil
November 2016
Cryptococcosis is a common opportunistic fungal infection caused by encapsulated yeast of the Cryptococcus genus, mainly by the Cryptococcus neoformans and Cryptococcus gattii species. The fungus is acquired through the inhalation of environmental propagules or, more rarely, through organ transplants in immune-compromised individuals. Domestic pigeons (Columba livia) are related to the infection, mainly as natural...
Identification and tracking of microorganisms from the biofilms of container walls used for water storage: Case of rural communities in Burkina Faso
November 2016
Safe drinking water is an important necessity for humans. In rural communities of Burkina Faso, many households use local containers for storing drinking water. During water storage, some microorganisms get attached to the surface walls of the containers to form a biofilm which can deteriorate drinking water quality over time. This study aimed at evaluating the attachment of indicator microorganisms to containers walls...
Selection of anti-wetting agents and photoprotectants compatible with a soybean weed control bioagent
November 2016
Adjuvants incorporation at formulations can positively influence the performance of bioagent control, contributing to preservation of the inoculum until being used. Adjuvants should not be toxic to pathogen and tests that evaluate the sensitivity of biologic products are essential. The aim of this study was to analyze the compatibility of the anti-wetting agents microcrystalline cellulose, anhydrous sodium carbonate,...
Storage behaviour and functionality of a heat stabilized starter derived from an African opaque sorghum beer
November 2016
Tchoukoutou is a traditional sorghum opaque beer produced and consumed in Benin. It is obtained from an uncontrolled fermentation of sorghum wort using a traditional starter called “kpete-kpete”. The present study assessed the effects of storage duration on the physicochemical and microbiological characteristics as well as on the functional properties of the heat stabilized starter produced from...
Characterization of arbuscular mycorrhizal fungi in apple (Malus domestica Borkh) growing area in Kashmir Himalaya (India): A case study of Bandipora district
November 2016
Rhizosphere soil samples and root pieces of 3 year old apple trees (var. Red delicious) were collected from 10 villages of district Bandipora of Kashmir Himalaya. Samples were processed for isolation of arbuscular mycorrhizal spores and their identification. On the basis of various morphological characters such as spore shape, colour, size, hyphal colour and with the help of International Collection of...
Effectiveness assessment of mouthwashes formulated from the essential oils of some beninese medicinal plants against oral germs
November 2016
Faced with the increasing growth of microorganism’s resistance to conventional antibiotics, the search of new bioactive molecules having pharmaceutical interest is more than ever committed. This study aimed to evaluate in vitro the effectiveness of seven mouthwashes formulated from essential oils of Ocimum gratissimum, Ocimum basilicum, Cymbopogon citratus, Clausena anisata, Lippia multifora, Eugenia caryophyllata...
Partial characterization of bacteriocin-like substance produced by probiotic Lactobacillus plantarum F12 isolated from Algerian children faeces
November 2016
The strain, Lactobacillus plantarum F12 with probiotic traits was isolated from Algerian healthy children faeces and identified by 16S rDNA sequencing. In this study, the antimicrobial activity and physicochemical properties of bacteriocin-like substance (BLS) produced by this strain were determined. Also, the bacteriocinogenic genes of plantaricin A, plantaricin J and plantaricin K were screened in this strain. The BLS...
Analysis of high levels of multidrug resistant Escherichia coli from healthy broiler chickens in Western Algeria
November 2016
One hundred and two Escherichia coli strains isolated from healthy broiler chickens collected from April 2012 to November 2014 in seven geographic areas of western Algeria were studied. Susceptibility pattern to 11 antimicrobial agents was determined by disk diffusion method as recommended by the Clinical Laboratory Standard Institute (CLSI). Antibiograms revealed high levels of resistance to quinolones and...
Enteric fever caused by Salmonella enterica serovar paratyphi A: An emerging health concern in Nepal
November 2016
Enteric fever is an invasive life-threatening systemic disease caused by the Salmonella enterica human-adapted serovars typhi and paratyphi. Increased incidence of infection with S. enterica serovar paratyphi A poses a significant health concern in some areas of the world. In this study, the incidence of enteric fever confirmed by isolation of Salmonella paratypi A or S. typhi from blood cultures of patients presenting...
In vitro antimicrobial activity of three medicinal plants of Ethiopia against some selected bacterial isolates
November 2016
Medicinal plants constitute natural source of antimicrobial drugs that will provide essential compounds to fight against disease. In this study, the antibacterial activity of ethanol extracts of Moringa stenopetala, Thymus serrulatus, and Terminalia brownii were investigated against selected pathogenic Gram positive and Gram negative bacteria. In vitro antibacterial activities of the ethanol extracts were tested at a...
Extended spectrum-β-lactamase producing Escherichia coli and Klebsiella pneumoniae causing urinary tract infection
November 2016
Extended spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae causing urinary tract infection (UTI) constitutes a significant therapeutic challenge. The aim of this study was to investigate the frequency of ESBL producing Escherichia coli and Klebsiella pneumoniae isolated from urine samples. Clinical isolates of multidrug resistant Gram negative rods (n=102) were taken from urine...
Cyclic depsipeptide producing fluorescent pseudomonads exerts antifungal activity against fungal pathogens of maize (Zea mays)
November 2016
Cyclic lipopeptides (CLPs) with antibiotic, biosurfactant producing fluorescent pseudomonads were isolated from sugar beet-maize intercropped in sandy loam soils at Maize Research Station, Vagarai, TNAU. Approximately 20 fluorescent pseudomonads from sandy loam soils were isolated by using two different growth media. The strains were distinguished based on their growth, CFU/g, fluorescence, and pigment production....
Production of fermented sweet potato flour using indigenous starter cultures
November 2016
The present study develop various microorganisms for starter culture purpose from fermented sweet potato and used the developed starters in the fermentation of sweet potato and products for consistent quality product with improved hygiene. Sweet potato was purchased from different markets and microorganisms were isolated and developed as starters. Comparative studies on different starters for the fermentation of sweet...
Microbiological evaluation of drinking water available in schools in Cruz das Almas, Brazil
November 2016
The current study assessed the drinkability of water in 25 schools in Cruz das Almas BA Brazil. Total coliforms, Escherichia coli, Enterococcus, mesophylls and psichrotrophic bacteria were evaluated, coupled with color, turbidity, free residual chlorine, dissolved oxygen and biochemical oxygen demands. Water samples were collected at four sites: the first tap within the supply network or within the alternative supply...
Physiochemical evaluation and liability of dromedary camel’s milk in combating various pathogens
November 2016
Dromedary camel’s milk is a natural source of probiotics; enzymes and secondary metabolites that have the ability to combat many pathogens. The aim of this study was to compare the effectiveness of filtered and non-filtered dromedary camel’s milk activity against various pathogens. Filtered and non-filtered (raw and boiled) dromedary milk was assessed against different pathogens by using agar well diffusion...
Sensory, microbiological and physico-chemical characterization of Klila, a traditional cheese made in the south-west of Algeria
November 2016
Klila, an Algerian cheese produced in steppe and mountainous areas, is proving increasingly popular with consumers. It is traditionally made with ewe, goat or cow milk, leading to a product with high dry matter content (> 90%). In this work, we have characterized three different Klila cheeses made with the three different milks using physico-chemical and microbiological parameters. A triangle test was also...
Soil enzyme activities and soil microbe population as influenced by long-term fertilizer management during the barley growth in Hunan Province, China
October 2016
The effects of long-term fertilizer management on soil enzyme activities and soil microbe population under double-cropping paddy fields in southern China was studied. The purpose of this study was to explore the changes of soil enzyme activities and soil microbe population as related to mineral fertilizer and manure and rice residue based on a long-term field experiment. The experiment was initiated in 1986 and...
Effect of gamma irradiation (Co60) in the control of Campylobacter sp. in chilled chicken (Gallus gallus) heart
October 2016
The present study aimed to evaluate the efficiency of the irradiation process in the control of Campylobacter spp. in chilled chicken heart samples, since this microorganism is related to the contamination of meat and chicken giblets and is responsible for enteritis in humans. The methodology and standards recommended by RDC no. 12 (Brazil, 2001) were applied for the bacteriological analyses. The chilled chicken heart...
Efficacy of biocontrol agents in the management of head rot of cabbage (Brassica oleracea var. capitata) caused by Sclerotinia sclerotiorum
October 2016
Head rot of cabbage caused by Sclerotinia sclerotiorum leads to rotting of fully matured cabbage heads in the field. In the present study the antagonistic effects of twenty Bacillus isolates was tested against S. sclerotiorum in vitro. Eight effective Bacillus isolates obtained from studies in vitro, commercial formulations of Trichoderma viride isolate TV-1 and Pseudomonas fluoroscens isolate Pf-1along with a fungicide...
Microbiological and molecular characterization of environmental mycobacterium strains isolated from the Buruli ulcer endemic and non-endemic zones in Côte d’Ivoire
October 2016
Mycobacterium ulcerans (MU), the causative agent of Buruli ulcer (BU), skin disease, is considered to be an environmental pathogen. The pathogenic virulence of MU is being linked to the expression of toxin called Mycolactone. Genetic analyses have shown the high diversity with variable number tandem repeats (VNTR) and mycobacterial interspersed repetitive units (MIRU) in M. ulcerans and in mycolactone producing...
External ocular bacterial infections among Sudanese children at Khartoum State, Sudan
October 2016
Ocular infections are widespread and they exert heavy burden on eye health. Virtually, any eye component can be infected by a diversity of bacteria. The present study was performed to determine the prevalence of external ocular bacterial infections and to find out antibiotic susceptibility pattern of bacterial isolates at eye care hospitals in Khartoum, Sudan. Two hundred and four corneal scrape and drained pus...
Pneumonia in Algerian Ouled Djellal sheep: Bacteriological study and macroscopic aspect of lung lesions
October 2016
Respiratory diseases in sheep are a multifactorial etiology syndrome, causing great economic losses. This study was carried out with the aim of establishing a bacteriological diagnosis of respiratory diseases in sheep, on the basis of lungs with macroscopic lesions taken from sheep slaughtered at the Blida abattoir. A total of 150 samples (swabs and lung parenchyma) from 75 Ouled Djellal sheep, aged six to twelve months...
Anti-biofilm activity of ibuprofen and diclofenac against some biofilm producing Escherichia coli and Klebsiella pneumoniae uropathogens
October 2016
Ibuprofen (IBU) and Diclofenac (DIC) are non-steroidal anti-inflammatory drug which have shown to have broad antimicrobial activity and limit biofilm formation. Aim of this study was to evaluate the effects of IBU and DIC against biofilm formation strong strains Escherichia coli and Klebsiella pneumoniae associated with urinary tract infections (UTIs). Additionally some virulence factors, antibiotic resistance, ESBL...
Effects of immunoglobulin Y (IgY) serum against plaque bacteria
October 2016
Dental caries is an infectious disease caused by plaque bacteria, especially Streptoccus mutans. Many studies were conducted to develop methods or material for dental caries prevention. It was using passive immunization that will produce specific antibodies. Yolk immunoglobulin (IgY) is antibody in the serum and egg yolk. This study aimed to determine the effect of IgY serum against the growth of dental plaque bacterial...
Campylobacter in sheep, calves and broiler chickens in the central region of Algeria: Phenotypic and antimicrobial resistance profiles
October 2016
The study was conducted in four slaughterhouses in Bouira Province, central region of Algeria. Campylobacter in the main food animals (sheep, calves and broilers) were studied to evaluate the prevalence, phenotypic characteristics and antibiotic susceptibility of isolated strains. Out of 200 sheep, 200 calves and 100 broilers swab samples collected. 150 strains were isolated and identified. A study of sensitivity to 14...
Bacterial association and oral biofilm formation: A bibliometric analysis
October 2016
Biofilms are complex aggregations of microorganisms formed in any situation where there is contact with superfaces solids, liquids or gases. This study aims to map the international scientific literature on the formation, bacterial adhesion, prevention and treatment of oral biofilms. There were 921 published articles in 274 different journals indexed in the Web of Science, in the 1991-2015 year period. These articles...
Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals
October 2016
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and a leading cause of hospital- acquired infections. Characterization of the isolates from different infection sites might help to control infections caused by the pathogen. The aim of the present work is to characterize P. aeruginosa isolates recovered from different clinical specimens at two hospitals in Cairo with regard to their antibiogram,...
Effectiveness of augmented consortia of Bacillus coagulans, Citrobacter koseri and Serratia ficaria in the degradation of diesel polluted soil supplemented with pig dung
October 2016
Laboratory studies were developed to compare the effectiveness of inoculated bacteria consortia and indigenous microorganisms on diesel-polluted soil for 18 days. Bacteria isolated from the unpolluted soil sample were: Pseudomonas spp. (LBI), Pseudomonas cepacia (LB5), Micrococcus luetus (LB2), Bacillus subtilis (LB3) and Bacillus cereus (LB4). Their ability to degrade different substrates were first studied by the...
Soil bacteriological pollution in pig farm vicinity: Assessment of bacterial dynamics and detection of antimicrobial resistance gene
October 2016
Emissions of biological contaminants (microbes) from intensive pig farming may cause environmental problems due to lack of proper waste management. This work was conducted to assess bacteriological pollution of soil in pig farm and to detect the presence of antibiotic resistance gene of the prevailing bacteria. Soil samples were collected from March to August 2013. The method included bacterial enumeration (10 -1 to 10...
Assessment of the microbial quality of some oral liquid herbal medicines marketed in Ile-Ife, South-western Nigeria
October 2016
The extensive use of herbal medicinal products in the treatment and management of disease states within communities in Ile-Ife, south western Nigeria has made it imperative to investigate the microbial quality of a sample of these products in the light of the standards prescribed by regulatory bodies. This study was therefore carried out to assess the microbial quality of locally prepared and unregistered herbal oral...
Modification of pathogenic microbiota and histology of gastrointestinal tract of Archachatina marginata (Swainson, 1821) by Carica papaya seed meal
October 2016
This study investigated the bactericidal efficacy of the ethanolic extract of Carica papaya seed on snail gastrointestinal tract (GIT) microbiota and associated histological changes. The bacterial isolates were characterized based on colony morphology, culture characteristics and biochemical tests. Each portion of the gut was further subjected to histological examination to ascertain the effect of this extract on the...
Development and evaluation of sundae-type “Coalhada†containing Lactobacillus paracasei and blueberry (Vaccinium ashei) preparation
October 2016
“Coalhada” is a type of fermented milk (made with Lactococcus lactis spp. lactis and Lactococcus lactis spp. cremoris) very popular in Brazil, which is widely consumed due to its pleasant texture and mild taste and aroma. Its acceptance is possible to increase by adding a fruit preparation. Fermented milk is the most common food matrix used for delivery probiotic bacteria, which are able to survive to the...
Impact of land use and soil types on arbuscular mycorrhizal fungal diversity in tropical soil of India
October 2016
A study was undertaken along land use gradients with different soil types in subtropical ecosystem of Northern India to evaluate the accurance of arbuscular mycorrhizal fungi (AMF). The gradients was from natural land with forest tree, farmer’s field under chemically managed, farmer’s field under organically managed and Industrial wasteland with five different plant species. We investigate the total AMF...
Prevalence and antimicrobial susceptibility profile of Salmonella species from ready-to-eat foods from catering establishments in Jigjiga City, Ethiopia
October 2016
Food-borne pathogens are the leading cause of morbidity and mortality in developing countries. Changes in eating habits, mass catering, unsafe food storage conditions and poor hygiene practices are major contributing factors to food associated illnesses. In Ethiopia, the widespread habit of ready-to-eat food consumption is potential cause of food borne illnesses. The present study aimed at investigating the prevalence...
Bacteria associated with sugarcane in Northeastern Brazil
October 2016
Sugarcane has a high demand in nitrogen, increasing costs and causing damages to the environment. It is necessary to find alternatives to reduce nitrogen fertilizers use. Diazotrophic bacteria have capacity to promote growth in grass with potential to fix N2 and solubilize inorganic phosphate. This study aimed to evaluate bacterial community associated with different sugarcane varieties in Northeastern Brazil, select...
Fungi toxic efficiency of some plant volatile essential oils against plant pathogenic fungi
October 2016
Plant essential oils have the potential to replace the synthetic fungicides in the management of different fungal diseases. Four different essential oils of eucalyptus (Eucalyptus globulus), citronella (Cymbopogon citrate), karanj (Pongamia pinnata) and neem (Azadirachta indica) were selected because of their high inhibitory activities against ten phytopathogenic fungi. The antifungal screening for all the four Eos...
Occurrence and susceptibility patterns of Campylobacter isolated from environmental water sources
October 2016
Environmental waters are established sources of Campylobacter infections in humans. The aim of this study was to assess the distribution and susceptibility profiles of Campylobacter species isolated from irrigation and domestic water sources in Ghana. Samples were pre-enriched with CCDA broth and isolated on mCCDA agar. Isolates were confirmed on API CAMPY kit and with the Kirby-Bauer disk diffusion method to determine...
Production and characteristics of bioflocculant TPT-1 from a consortium of Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2
October 2016
The combination of microorganisms in consortia enhances high bioflocculant yields. The study aimed at producing and characterising bioflocculant from Bacillus pumilus JX860616 and Alcaligenes faecalis HCB2. Bioflocculant TPT-1 was obtained through ethanol extraction after optimum conditions were established. The characteristics of the bioflocculant TPT-1 were obtained by scanning electron microscope equipped with...
Effect of temperature, pH and substrate composition on production of lipopeptides by Bacillus amyloliquefaciens 629
September 2016
The lipopeptides surfactin, fengycin and iturin produced by Bacillus species have diverse ecological roles, including antimicrobial activity, induced systemic resistance in plants against pathogens and plant colonization by the producing strain. The conditions that govern both lipopeptide production and plant colonization are not fully understood. The present study investigated the role of growth media, temperature and...
Isolation, screening and statistical optimizing of L-methioninase production by Chaetomium globosum
September 2016
Egyptian soil sample was screened for isolation methioninlytic fungi by a rapid plate assay procedure. Eighteen strains of different isolated fungi were screened quantitavely for their L-methioninase activity. Chaetomium globosum was the most efficacious isolate and a dematiaceous filamentous fungi, it was identified at the molecular level by ribotyping 18S rRNA along with the biochemical characterization, and...
Microbial community structure and chemical composition from dark earth in a native archaeological site of the lower Amazon
September 2016
The microbial community structure from dark earth in a native archaeological site of the Lower Amazon was analyzed by PCR-DGGE, using 16S rRNA gene for prokaryote population and 18S rDNA and ITS regions (using clamp GC) for the eukaryote population. The bands were excised from gel and re-amplified for sequencing. The diversity found according to the region of amplification showed same profiles for the two primers pairs....
Screening endophytic actinobacteria with potential antifungal activity against Bipolaris sorokiniana and growth promotion of wheat seedlings
September 2016
Actinobacteria secrete substances that limit or inhibit the growth of plant pathogenic fungi and may be used in the biocontrol of these microorganisms. The aim of this study was to characterize physiological and enzymatic activity of endophytic actinobacteria, evaluate their antifungal activity against Bipolaris sorokiniana root colonization, and evaluate their efficiency in promoting the growth of wheat seedlings....
Page 14 of 105, showing 50 records out of 5211 total, starting on record 651, ending on 700
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