African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12268

AJB Articles

Osmotic stress upregulates the transcription of thiamine (vitamin B1) biosynthesis genes (THIC and THI4) in oil palm (Elaies guineensis)

July 2016

Thiamine or vitamin B1 comprises a pyrimidine moiety and a thiazole moiety. Thiamine pyrophosphate (TPP), the active form of thiamine, acts as a cofactor for various major enzymes, for example, transketolase (TK), α-ketoglutarate dehydrogenase (KGDH) and pyruvate dehydrogenase (PDH). It is suggested that TPP plays another important role, which is protecting plants against abiotic and biotic stresses such as...

Author(s): Wong Sook Yee, Syamimi Diyana Abdul Aziz and Zetty Norhana Balia Yusof

Identification and analysis of cassava genotype TME3 bacteria artificial chromosome libraries for characterization of the cassava mosaic disease

July 2016

Cassava is an economically important crop in sub-Saharan Africa; however, its yield potential is constrained by cassava mosaic disease (CMD) infection. Classical genetics and biotechnology are being harnessed to overcome the disease and secure yields for farmers. The CMD2 resistance locus flanked by three simple sequence repeats (SSR) markers and one sequence characterized amplified region (SCAR) marker were mapped in...

Author(s): Paul Kuria,, Elijah Ateka, Douglas Miano, Justus Onguso and Nigel J. Taylor

Plant latex lipase as biocatalysts for biodiesel production

July 2016

Industrial-scale processes currently developed make use of chemical catalysis processes that are highly efficient but require very complex product purification steps. Enzymatic catalysis through plant lipases as biocatalysts is an alternative which, in contrast to chemical catalysis processes, appeared simple to perform, and can be done at low investment cost. Although microbial lipases have been extensively studied,...

Author(s): Mouaïmine Mazou, Andriano Jospin Djossou, Fidèle Paul Tchobo, Pierre Villeneuve and Mohamed M. Soumanou

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA®

July 2016

The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semi-solid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and...

Author(s): Luís Gonzaga Gutiérrez, Rodolfo López-Franco and Tito Morales-Pinzón

Characterization of α-amylase produced by the endophytic strain of Penicillium digitatum in solid state fermentation (SSF) and submerged fermentation (SMF)

July 2016

α-Amylases are enzymes responsible for breaking the α-1,4 bond in polysaccharides with three or more glucose units, occupying the second place in the most widely employed enzymes in industry in the world. The objective of this study was to compare the yields of α-amylase produced by the endophytic fungus, Penicillium digitatum, strain D1-FB, isolated from Baccharis dracunculifolia D.C. (Asteraceae),...

Author(s): Sideney Becker Onofre, Dirceu Abatti, Douglas Refosco, Amarildo Antonio Tessaro, Jean Alisson Becker Onofre and Alessandra Buss Tessaro

Development of bio-hybrid material based on Salmonella Typhimurium and layered double hydroxides

July 2016

The immobilization of a whole microbial cell is an important process used in nanotechnology of biosensors and other related fields, especially the development of bio-hybrid materials based on live organisms and inorganic compounds. Here, we described an essay to develop a bio-hybrid material based on Salmonella Typhimurium cells and layered double hydroxides (LDH). The synthetic clays have a good capacity to be a host...

Author(s): Slah Hidouri, Nadia Messaoudi, Mouadh Mihoub, Zouhair M. Baccar and Ahmed Landoulsi

Optimization of biomass production of Acetobacter pasteurianus KU710511 as a potential starter for fruit vinegar production

July 2016

The objective of the present work was first the isolation of novel acetic acid bacteria strains from natural Moroccan habitats, and then, the evaluation of their ability to produce microbial starters for vinegar production on a large scale. The strains were isolated from figs, dates, cactus, and traditional fruit vinegars. Four strains, selected from a total of 63 isolates, were confirmed as belonging to Acetobacter...

Author(s): Majid Mounir, Rasoul Shafiei, Raziyeh Zarmehrkhorshid, Allal Hamouda, Philippe Thonart, Frank Delvigne and Mustapha Ismaili Alaoui

Effect of pond depth and lining plastic color on growth and nitrogen fixing capacity of the cyanobacteria, Anabaena sp. E3

July 2016

Cyanobacteria are a cheap source of nitrogen and quite suitable for farmers of developing countries. Although, they live in a diverse range of environments, different environmental variables influence their nitrogen fixing ability. Thus, this study evaluated the effect of pond depth and lining plastic colors on nitrogen fixing capacity of Anabaena species strain E3. Factorial combinations of four pond lining plastic...

Author(s): Ashebir Tsedeke, Tulu Degefu, Endalkachew Wolde-Meskel and Jessica Davis

Plant growth promoting rhizobacteria: Beneficial effects for healthy and sustainable agriculture

July 2016

It is unanimously admitted that the chemical fertilizers and pesticides used in modern agriculture create a real environmental and public health problems. One of the promising solutions to substitute these agrochemicals products is the use of bio-resources, including plant growth promoting rhizobacteria (PGPR). The PGPR focused more and more scientific attention in recent decades. These rhizospheric bacteria colonize...

Author(s): Pacôme Agossou Noumavo, Nadège Adoukè Agbodjato, Farid Baba-Moussa, Adolphe Adjanohoun and Lamine Baba-Moussa

Whole genome homology-based identification of candidate genes for drought tolerance in sesame (Sesamum indicum L.)

July 2016

Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in arid and semi-arid regions with occurrence of unpredictable drought which is one of the major constraints of its production. However, the lack of gene resources associated with drought tolerance hinders sesame genetic improvement towards this osmotic stress. The present research aimed at identifying candidate genes associated...

Author(s): Komivi Dossa,, Marème Niang, Achille E. Assogbadjo, Ndiaga Cissé and Diaga Diouf

Effect of substrate on the growth, nutritional and bioactive components of Pleurotus ostreatus and Pleurotus florida

July 2016

Mushrooms are increasingly being recognized as important food products for their significant role in human health, nutrition and disease. This study was carried out with the aim of comparing the effect of substrate on growth, determining nutritional and bioactive components of two oyster mushroom, Pleurotus ostreatus and Pleurotus florida. A completely randomized block design with two treatments replicated three times...

Author(s): Kinge, T. R., Adi, E. M., Mih, A. M., Ache, N. A. and Nji, T. M.

Ameliorative effect of Lentinus squarrosulus mycomeat against Pseudomonas aeruginosa infection using albino rat as animal model

June 2016

The increasing awareness of inherent therapeutic and prophylactic benefits of some higher fungi and their products has been the recent trend for improving a healthy vigour. Mycomeat is a mushroom derived ‘meat-like’ product produced using solid state fermentation technique. Previous research in this group revealed mycomeat as a potentially useful neutraceutical. This study seeks to explore the potential of...

Author(s): Bamigboye C. O. and Oloke J. K.

Efficacy of chitosan supported organic acaricide extract from Melia azedarach leaves on Rhipicephalus (Boophilus) microplus ticks

June 2016

The Rhipicephalus (Boophilus) microplustick control is mainly performed by chemicals products, but organic acaricides use has higher advantages compared to conventional products. Brazilian cerrado native plants are known for their bioactive potential. Due to this fact, the objective of this study is to evaluate the acaricide action of native cerrado specie, Meliaazedarach, known as Santa Barbara in the tick control....

Author(s): Priscila Fernanda Pereira Barbosa, Pablo Peres de Mendonça, Rômulo Davi Albuquerque Andrade, Ana Carolina Ribeiro Aguiar, Andréa Rodrigues Chaves and Fabiano Guimarães Silva

Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment

June 2016

Proteolytic activity of a Streptomyces sp. strain isolated from Ezzemoul saltpans (Algeria) was studied on agar milk at three concentrations. The phenotypic and phylogenetic studies of this strain show that it represents probably new specie. The fermentation is carried out on two different media, prepared at three pH values. The results showed the presence of an alkaline protease with optimal pH and temperature of 8 and...

Author(s): Boughachiche Faiza, , Rachedi Kounouz, Duran Robert,  Lauga Béatrice,  Karama Solange,  Bouyoucef  Lynda, Boulezaz Sarra, Boukrouma Meriem, Boutaleb Houria and Boulahrouf Abderrahmane

Recovery of silver from used X-ray film using alkaline protease from Bacillus subtilis sub sp. subtilis

June 2016

Silver is an important industrial metal used in several areas such as photographic and x-ray films, jewelries, silver wares and electronic objects. Silver is used for photographic film/x-ray film because of its matchless quality as a light-sensitive material for making a photographic image. Silver is not destroyed in the photographic process and it can be reused and recovered. Results have proven that, bacterial...

Author(s): Amira Hassan Al-Abdalall and Eida Marshid Al-Khaldi

Flow cytometry approach for studying the interaction between Bacillus mojavensis and Alternaria alternata

June 2016

Tomato, Solanum lycopersicum is one of the most important vegetable crops consumed in Algeria. Tomato crops are often infected by Alternaria alternata, which causes early blight disease. Chemical pesticides are intensively used to protect this plant, which lead to environmental pollution that might endanger animal and human health. The main objective of this study is to select potential biocontrol agents from arid soil...

Author(s): Asma MILET, Noreddine Kacem CHAOUCHE, Laid DEHIMAT, Asma Ait KAKI, Mounira Kara ALI and Philippe THONART

Electrospun fibers for wound healing and treatment of hyperglycemia: A review

June 2016

The use of electrospun fibers in diabetic wound healing assays represents an extremely important topic for investigation. Electrospun fibers have been applied in the immobilization of drugs, allowing sustained and controlled release of active materials. This review aimed to summarize the state-of-art in the application of electrospun fibers on diabetes, hyperglycemic and diabetic ulcers treatment. Regarding the diabetes...

Author(s): Soares J. M. D., Silva J. C., Almeida J. R. G. S., Quintans-Júnior L. J. and de Oliveira H. P.

Predominance of N-acetyl transferase 2 slow acetylator alleles in children less than ten years experiencing adverse treatment events following artemisinin-based combination therapy in North and South West Regions of Cameroon

June 2016

The human N-acetyltransferase II (NAT2) gene may vary between individuals resulting in variability in the incidence of adverse drug reactions. We set out in this adhoc analysis to determine the distribution of allele frequencies of NAT2 gene variants among children less than ten years treated with artemisinin-based combinations in Cameroon. Children with uncomplicated malaria were deparasitized with artemisinin based...

Author(s): Jean P. C. Kengne,  Akindeh M. Nji, Innocent M. Ali,, Olivia  A. Achonduh, Ekollo M. Aristid, and Wilfred F. Mbacham,

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

June 2016

Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidal properties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog’s (MS) medium. A disinfection protocol was...

Author(s): Evelyn Duarte, Pedro Sansberro and Claudia Luna

A endophytic fungus, Ramichloridium cerophilum, promotes growth of a non-mycorrhizal plant, Chinese cabbage

June 2016

A fungal endophyte, Ramichloridium cerophilum, was identified as a Class 2 endophytes species obtained from the leaf of common sowthistle (Sonchus oleraceus L). This fungus was found to grow endophylically in the roots of Chinese cabbage seedlings. Light microscopy of cross-sections of colonized Chinese cabbage roots showed that the fungus penetrates through the outer epidermal cells, grows further into the inner...

Author(s): Ling Xie, Erika Usui and Kazuhiko Narisawa

Genetic diversity in two populations of Limicolaria aurora (Jay, 1839) from two ecological zones in Nigeria

June 2016

Limicolaria aurora belongs to the group of land snails commonly called garden snails. This study seeks to use shell morphology and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) to examine gross morphological differences in populations of L. aurora from New Bussa (guinea savannah) and Benin City (tropical rain forest) in Nigeria and possibly delimit the populations into sub species. A total of one...

Author(s): Michael Olufemi AWODIRAN and Zaccheaus Omololu OGUNJOBI

Genetic polymorphism of bone morphogenetic protein receptor 1B (BMPR-1B) gene and its association with litter size in Indonesian fat-tailed sheep

June 2016

The Indonesian fat-tailed sheep (IFTS) is a local sheep that has been long time raised and well adapted to the extreme environments of Lombok Island. The present study was conducted to determine the polymorphism of bone morphogenetic protein receptor 1B (BMPR-1B) gene and its association with litter size in the IFTS breed by employing forced polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...

Author(s): Maskur M., Tapaul R., and Kasip L.

Does the transgenic Cry1Ac toxin adversely affect the population dynamics of floral-visiting insects in soybean crop?

June 2016

One of the strategies of Integrated Pest Management (IPM)-soybean is the use of genetically modified plants. However, there are concerns about the unknown effects associated with this technology on non-target organisms. The objective of this study was to determine the population dynamics of species of floral-visiting insects in Bacillus thuringiensis (Bt) and non-Bt soybean. In the experiment, two soybean treatments...

Author(s): Oliveira, F. and Fernandes, M. G.

Morpho-phenological diversity among natural populations of Medicago polymorpha of different Tunisian ecological areas

June 2016

Medicago polymorpha is a herbaceous legume that can be a useful pasture plant, in particular, in regions with a Mediterranean climate. The genetic variation in 120 lines of M. polymorpha sampled from five regions in Tunisia was characterized on the basis of 16 morpho-phenological characters. Results from analysis of variance (ANOVA) showed that differences among populations and lines existed for all traits, with...

Author(s): Mounawer Badri, Najah Ben Cheikh, Asma Mahjoub and Chedly Abdelly

Morph-physiological responses to water deficit in parental genotypes of Medicago truncatula recombinant inbred lines

June 2016

Medicago truncatula is an omni-Mediterranean species grown as an annual forage legume. In addition to its small genome size and simple genetics, M. truncatula harbors several attributes which make it an attractive model legume. In this study, we investigated morphological and photosynthetic responses to water deficit in parental genotypes of M. truncatula recombinant inbred lines. Ten parental lines were...

Author(s): Mounawer Badri, Saoussen Mahfoudh and Chedly Abdelly

Drought tolerance in transgenic tropical maize (Zea mays L.) by heterologous expression of peroxiredoxin2 gene-XvPrx2

June 2016

Transformation of a tropical maize inbred genotype (CML144) with the Xerophyta viscosa peroxiredoxin2 (XvPrx2) gene was reported. The protective role of peroxiredoxin2 against the damage resulting from reactive oxygen species (ROS) under dehydration stress was further determined. Successful integration of XvPrx2 gene into maize we achieved and recovered 10 independent transgenic events. Transformation and regeneration...

Author(s): Miccah S. Seth, Leta T. Bedada, Steven M. Runo, Mathew P. Ngugi, Revel Iyer, Jennifer A. Thomson, Emmarold E. Mneney and Richard O. Oduor

Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites

June 2016

The genetic variability of 28 sorghum genotypes of known senescence phenotype was investigated using 66 SSR markers well-distributed across the sorghum genome. The genotypes of a number of lines from breeding programmes for stay-green were also determined. This included lines selected phenotypically for stay-green and also RSG 03123, a marker-assisted backcross progeny of R16 (recurrent parent) and B35 (stay-green...

Author(s): Isaac K. A. Galyuon, R. Madhusudhana, Andrew K. Borrell, Tom C. Hash and Catherine J. Howarth

Sapucaia nuts (Lecythis pisonis) modulate the hepatic inflammatory and antioxidant metabolism activity in rats fed high-fat diets

June 2016

Lecythis pisonis Cambess is commonly known as “sapucaia” nut. Previous studies show that it is rich in unsaturated fatty acids and in antioxidant minerals. The aim of the present study was to assess the antioxidant and anti-inflamatory effects of this nut after its introduction into a control (AIN-93G) or high-fat diet in Wistar rats. The animals were divided into four groups: a control diet, the same...

Author(s): Marcos Vidal Martins, Izabela Maria Montezano de Carvalho, Mônica Maria Magalhães Caetano, Renata Celi Lopes Toledo, Antônio Avelar Xavier and José Humberto de Queiroz

Application of biotechnology for the domestication of Dacryodes edulis (G. Don) H. J. Lam in Cameroon: A review

June 2016

Cultivation of Dacryodes edulis (G. Don) H. J. Lam commonly known as safou to improve the livelihood of the local population has been growing rapidly in the range of occurrence of safou and recent emergence of the market chain introduced by the World Agroforestry Center (ICRAF) experts has stimulated this further. Domestication through conventional genetic improvement (breeding) of safou has relied on phenotype...

Author(s): TCHATCHOUA Dorothy Tchapda, Tchoundjeu Zac and CASPA Roseline Gusua

Growth performance and nutrient utilization of Clarias gariepinus fed with different dietary levels of processed cassava leaves

June 2016

The growth performance of Clarias gariepinus fed with different dietary levels of processed cassava leaves and their nutrient utilized were studied for a period of 24 weeks. One hundred and forty four experimental Catfish (Clarias gariepinus) with 0.75±0.20 g mean weight and 3.9±0.31 cm mean total length were collected from Aqua-fish Consult, Awka, Anambra State, Nigeria. Eighteen rectangular plastic tanks...

Author(s): Odo Gregory E., Agwu Juliana E., Nweze Nkechi O., Clement Sunday, Alfreda Nwadinigwe O., Onyishi Grace, Uche Nzekwe, Ajuziogu Godswill, Ikegbunam Clara, Nelson Ossai I., Ezea Osita C., Osayi Emmanuel E., Onyeke Christopher C. and Eneje Victor

Influence of aluminum on root growth and of anatomy Stenocalyx dysentericus (DC.) O. Berg

June 2016

This study aimed to evaluate the effect of aluminum (Al) on root growth and root anatomical structure of Stenocalyx dysentericus seedlings. Newly emerged plants were grown in simple solution composed of 0.1 µM of CaCl2.2H2O and five Al concentrations of 0, 150, 300, 600, and 1200 μM for 37 days in a hydroponic system. Subsequently, the seedlings were evaluated for root growth, relative root elongation, and...

Author(s): Rodrigues, A. A., Vasconcelos Filho, S. C., Rodrigues, D. A., Rodrigues, C. L., Sales, J. F. and Vital, R. G.

Physicochemical analysis of cellulose from microalgae Nannochloropsis gaditana

June 2016

Nannochloropsis gaditana is a microalgae belonging to the class of Eustigmatophyceae. This particular microalga is the most studied species. For its richness in lipids, it is used for the biodiesel production. The aim of this work is to advance another important metabolite which is cellulose. This represents 25% of the dry weight of the N. gaditana. A low concentration of NaOH (2%) and sodium hypochlorite (6%) allowed...

Author(s): BABA HAMED Samia, BABA HAMED Mohamed Bey, KASSOUAR Schéhérazade and ABI AYAD Sidi– Mohammed El - Amine

Effect of different microencapsulation materials on stability of Lactobacillus plantarum DSM 20174

June 2016

The aim of this work was to investigate the effect of different microencapsulation materials on the stability of probiotic bacterium (Lactobacillus plantarum DSM 20174). Microencapsulation methods with alginates were carried out using sodium chloride, canola oil, olive oil, and chitosan. The recorded data showed that the encapsulated probiotic bacterium was more stable compared with free cells. Olive oil capsules...

Author(s): Lulwah Y. Al-Furaih, Ibtisam M. Ababutain, Azzat B. Abd-El-Khalek and Ahmed M. Abdel-Salam

Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil

June 2016

Endophytic and epiphytic fungi isolated from Eichhornia crassipes (Mart.) Solms and Cyperus ligularis L., macrophytes collected from oil-contaminated waters, were studied to assess their potential for producing biosurfactants; the most promising ones were identified by means of the rDNA region sequencing. In the selection, in the hydrocarbonate biodegradation activity, 2.6-indophenol (DCPIP) in oil-added Bushnell-Haas...

Author(s): João Marcelo Silva Lima, José Odair Pereira, Ieda Hortêncio Batista, Pedro de Queiroz Costa Neto, Jucileuza Conceição dos Santos, Solange Pires de Araújo, Mozanil Correia Pantoja, Adolfo José da Mota and João Lúcio de Azevedo,

Foot-and-mouth disease (FMD) prevalence and exposure factors associated with seropositivity of cattle in north-central, Nigeria

June 2016

This study was designed to determine the seroprevalence and risk factors associated with foot-and-mouth disease (FMD) seropositivity in north central, Nigeria. A cross-sectional study was undertaken from February 2013 to April 2014 using 1206 sera from 150 herds collected by multi-stage and random sampling methods. Pre-tested questionnaire were also administered to participating farmers to collect information on the...

Author(s): Wungak, Y. S., Olugasa, B. O., Ishola, O. O., Lazarus, D. D. and Ularamu, G. H.

GhNAC18, a novel cotton (Gossypium hirsutum L.) NAC gene, is involved in leaf senescence and diverse stress responses

June 2016

GhNAC18 is a novel NAC gene that was isolated from cotton (Gossypium hirsutum L.). The full-length cDNA was 1511 bp including an open reading frame of 1260 bp in length and encodes a protein of 419 amino acids. With qRT-PCR analysis, GhNAC18 was downregulated during natural and dark-induced senescence, implicating this gene as anti-aging gene in cotton. Analysis of its promoter revealed a group of putative cis-acting...

Author(s): Ondati Evans, Lingling Dou, Yaning Guo, Chaoyou Pang, Hengling Wei, Meizhen Song, Shuli Fan and Shuxun Yu

Fatty acids and amino acids contents in Scomber scombrus fillets from the South East of Tunisia

June 2016

Proximate composition, fats and amino acids of Atlantic Mackerel fillets (Scomber scombrus) from the South East of Tunisia in different seasons, were analyzed in order to assess nutritive characteristics of this species. Samples were collected monthly from Zarzis fishing port located in the South-East of Tunisia. Total fats and protein contents varied significantly (P<0.05) according to the season. The highest values...

Author(s): EL Oudiani Salma, Darej Cyrine and Moujahed Nizar

Biochemical and hematological profile of different breeds of goat maintained under intensive production system

June 2016

The aim of the study was to monitor the health and nutritional status of Kuwaiti’s Aradi and exotic Damascus and Barbari goat breeds raised under an intensive system of production through the determination of biochemical parameters and hematology profiles. The study was conducted during the summer season and blood samples were collected from lactating does and Damascus bucks. According to the findings of the...

Author(s): Samira Ahmed Mohammed, Mohammed Abdur Razzaque, Anaam E. Omar, Sheeba Albert, and Waleed M. Al-Gallaf

Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeorohita): Extraction and characterization

June 2016

Chitosan is a naturally available biopolymer. It has been prepared by alkaline N-deacetylation process of shrimp (Crangon crangon) chitin and fish (Labeorohita) chitin. The physico-chemical properties such as the degree of deacetylation (DD), solubility, water binding capacity, fat binding capacity and chitosan yield have indicated that shrimp shell and fish scale waste are good sources of chitosan. The deacetylation...

Author(s): Suneeta Kumari, Rath P. and Sri Hari Kumar A.

In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

June 2016

Embracing micro-propagation method for large scale production of plantlets and also for protection of appropriate germplasm is a prerequisite that needs to be undertaken in order to develop a rapid in vitro regeneration protocol for Ocimum sanctum L. Shoot tips as well as nodal segments were subjected to numerous shoots inducement. Explants were cultured on Murashige and Skoog Basal Medium (MS) supplemented for...

Author(s): Mohammad Abu Hena Mostofa Jamal, Imdadul Hoque Sharif, Md. Mostofa Shakil, A. N. M. Rubaiyath-Bin Rahman, Nilufa Akhter Banu, Md. Rezuanul Islam and Md. Nazmuzzaman

An improved plating assay for determination of phage titer

June 2016

Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for...

Author(s): Liu Yang, Chao Li, Shao-Qin Zhai, Hua Zheng, Li-Zhi Fu, and Da-Jun Li,

Improving the optimum yield and growth of Chlamydomonas reinhardtii CC125 and CW15 using various carbon sources and growth regimes

June 2016

Chlamydomonas reinhardtii CC125 (wild type) and CW15 (cell wall mutants) were feed up on solid and liquid Tris phosphate (TP) media with various concentrations of acetate, glycerol(10-100 mM) or methanol (0.01-718 mM) and cultivated under phototrophic, mixotrophic and heterotrophic conditions. Use of 10 and 35 mM acetate and 10 and 50 mM glycerol under constant 38 µE m−2 s−1 light illumination...

Author(s): Navid Taghavi and Gary Robinson

Variation in levels and removal efficiency of heavy and trace metals from wastewater treatment plant effluents in Cape Town and Stellenbosch, South Africa

June 2016

This study focused on one year monitoring campaign to monitor the occurrence and removal of Endocrine Disruptive Metals (EDMs) and trace metals from selected wastewater treatment plants (WWTPs) in Stellenbosch and Cape Town. Composite water samples were collected from the WWTPs from January 2010 to December 2010 on a quarterly basis and concentrations determined using inductively coupled plasma-mass spectrometry...

Author(s): Olujimi, O. O., Fatoki, O. S., Odendaal, J. P., Daso, A. P. and Oputu, O. U.

Genetic diversity studies on selected rice varieties grown in Africa based on aroma, cooking and eating quality

June 2016

Rice grain quality is an important factor that has a great influence on its market value and consumer acceptance. It is determined by three parameters controlling the cooking and eating qualities of rice (amylose content, gelatinization temperature and gel consistency) and by the aroma, which becomes a criterion increasingly preferred by consumers. Molecular characterization of specific genomic regions of rice genotypes...

Author(s): Koffi Kibalou Palanga, Karim Traore, Kofi Bimpong, Muhammad Jamshed and Mtawa A. P. Mkulama

Genetic diversity and population structure among sorghum (Sorghum bicolor, L.) germplasm collections from Western Ethiopia

June 2016

The Western Ethiopian region harbors a unique set of sorghum germplasm adapted to conditions not conventional to sorghums grown in other parts of the world. Accessions from the region possess unique resistance to multiple leaf and grain diseases. This study is aimed at exploring the extent of genetic variation and population structure among accessions of this region. A total of 123 accessions comprising 111 from Western...

Author(s): Dilooshi K. Weerasooriya, Frank R. Maulana, Ananda Y. Bandara, Alemu Tirfessa, Amsalu Ayana, Girma Mengistu, Kinde Nouh and Tesfaye T. Tesso

Optimal concentration of selective agents for inhibiting in vitro growth of Urochloa brizantha embryogenic calli

June 2016

The ability to distinguish transgenic cells of untransformed cell mass is a key step for the production of transgenic plants. Thus, the use of selection marker genes for identification of genetically modified plants is necessary. The aim of this study was to determine the optimal concentration of four selective agents (kanamycin, hygromycin, phosphinothricin and mannose) to inhibit in vitro growth of Urochloa brizantha...

Author(s): Alyne Valéria Carrion Pereira, Luiz Gonzaga Esteves Vieira and Alessandra Ferreira Ribas

Bioactivity of mangrove humic materials on Rizophora mangle and Laguncularia racemosa seedlings, Brazil

June 2016

The mangrove sediments are rich in organic matter and humic substances, responsible for important functions such as reducing the toxicity of heavy metals, nutrient stabilization, serving as drain to atmospheric carbon and increasing the plant growth. In this study, we observed the effect of humic substances, humic acids and fulvic acids isolated from sediments of a mangrove forest, sampled from the Municipal Ecological...

Author(s): Leonardo Barros Dobbss, André Luiz Paier Barroso, Alessandro Coutinho Ramos, Karla Stéphanie Nunes Torrico, Fabíola Schunk de Souza Arçari and Daniel Basílio Zandonadi

Comparative assessment of different poultry manures and inorganic fertilizer on soil properties and nutrient uptake of maize (Zea mays L.)

June 2016

This study investigated soil physicochemical properties and nutrient uptake of a drought-tolerant maize variety (DT-SR-WC2) in a derived savanna zone of Southwestern Nigeria when different composted poultry manures (CPM) and inorganic fertilizer (IF) were applied. This was with a view to determining the essential trace elements and nutritional quality of the test crop. The experiment was a randomized complete block...

Author(s): Moses B. Adewole, Olusegun M. Otitoju and Aderonke A. Okoya

Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment

June 2016

In recent years, intense interest has been drawn to the effects of radio-frequency electromagnetic field (RF-EMF) on reproduction. To explore the effects of RF-EMF on sperm maturation in epididymis microenvironment, 24 male Sprague Dawley (SD) rats were randomly divided into three exposed groups (SAR 1, 2 and 4 W/kg) and one sham group. The rats in exposed group were exposed to 1.84 GHz RF-EMF for 5 days (1 h/day)....

Author(s): Guangzhou An, Jing Li, Shenglong Xu, Tao Zhao, Kangchu Li, Jiajin Lin, Yan Zhou, Lihua Zeng, Guozhen Guo and Gui-Rong Ding

Investigation of yeast genes possibly involved in mtDNA stability using the nematode Caenorhabditis elegans

June 2016

Screening of Caenorhabditis elegans genes possibly involved in the mitochondrial genome maintenance was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to yeast genes known to be involved in mtDNA stability but of unknown molecular function or to identify transient components that could play important role on the stability of mtDNA...

Author(s): Addo M. G., Cossard R., Pichard D., Obiri-Danso K., Rötig A. and Delahodde A.

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