African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

AJB Articles

June 2016

Application of biotechnology for the domestication of Dacryodes edulis (G. Don) H. J. Lam in Cameroon: A review

Cultivation of Dacryodes edulis (G. Don) H. J. Lam commonly known as safou to improve the livelihood of the local population has been growing rapidly in the range of occurrence of safou and recent emergence of the market chain introduced by the World Agroforestry Center (ICRAF) experts has stimulated this further. Domestication through conventional genetic improvement (breeding) of safou has relied on phenotype...

Author(s): TCHATCHOUA Dorothy Tchapda, Tchoundjeu Zac and CASPA Roseline Gusua

June 2016

Growth performance and nutrient utilization of Clarias gariepinus fed with different dietary levels of processed cassava leaves

The growth performance of Clarias gariepinus fed with different dietary levels of processed cassava leaves and their nutrient utilized were studied for a period of 24 weeks. One hundred and forty four experimental Catfish (Clarias gariepinus) with 0.75±0.20 g mean weight and 3.9±0.31 cm mean total length were collected from Aqua-fish Consult, Awka, Anambra State, Nigeria. Eighteen rectangular plastic tanks...

Author(s): Odo Gregory E., Agwu Juliana E., Nweze Nkechi O., Clement Sunday, Alfreda Nwadinigwe O., Onyishi Grace, Uche Nzekwe, Ajuziogu Godswill, Ikegbunam Clara, Nelson Ossai I., Ezea Osita C., Osayi Emmanuel E., Onyeke Christopher C. and Eneje Victor

June 2016

Influence of aluminum on root growth and of anatomy Stenocalyx dysentericus (DC.) O. Berg

This study aimed to evaluate the effect of aluminum (Al) on root growth and root anatomical structure of Stenocalyx dysentericus seedlings. Newly emerged plants were grown in simple solution composed of 0.1 µM of CaCl2.2H2O and five Al concentrations of 0, 150, 300, 600, and 1200 μM for 37 days in a hydroponic system. Subsequently, the seedlings were evaluated for root growth, relative root elongation, and...

Author(s): Rodrigues, A. A., Vasconcelos Filho, S. C., Rodrigues, D. A., Rodrigues, C. L., Sales, J. F. and Vital, R. G.

June 2016

Physicochemical analysis of cellulose from microalgae Nannochloropsis gaditana

Nannochloropsis gaditana is a microalgae belonging to the class of Eustigmatophyceae. This particular microalga is the most studied species. For its richness in lipids, it is used for the biodiesel production. The aim of this work is to advance another important metabolite which is cellulose. This represents 25% of the dry weight of the N. gaditana. A low concentration of NaOH (2%) and sodium hypochlorite (6%) allowed...

Author(s): BABA HAMED Samia, BABA HAMED Mohamed Bey, KASSOUAR Schéhérazade and ABI AYAD Sidi– Mohammed El - Amine

June 2016

Effect of different microencapsulation materials on stability of Lactobacillus plantarum DSM 20174

The aim of this work was to investigate the effect of different microencapsulation materials on the stability of probiotic bacterium (Lactobacillus plantarum DSM 20174). Microencapsulation methods with alginates were carried out using sodium chloride, canola oil, olive oil, and chitosan. The recorded data showed that the encapsulated probiotic bacterium was more stable compared with free cells. Olive oil capsules...

Author(s): Lulwah Y. Al-Furaih, Ibtisam M. Ababutain, Azzat B. Abd-El-Khalek and Ahmed M. Abdel-Salam

June 2016

Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil

Endophytic and epiphytic fungi isolated from Eichhornia crassipes (Mart.) Solms and Cyperus ligularis L., macrophytes collected from oil-contaminated waters, were studied to assess their potential for producing biosurfactants; the most promising ones were identified by means of the rDNA region sequencing. In the selection, in the hydrocarbonate biodegradation activity, 2.6-indophenol (DCPIP) in oil-added Bushnell-Haas...

Author(s): João Marcelo Silva Lima, José Odair Pereira, Ieda Hortêncio Batista, Pedro de Queiroz Costa Neto, Jucileuza Conceição dos Santos, Solange Pires de Araújo, Mozanil Correia Pantoja, Adolfo José da Mota and João Lúcio de Azevedo,

June 2016

Foot-and-mouth disease (FMD) prevalence and exposure factors associated with seropositivity of cattle in north-central, Nigeria

This study was designed to determine the seroprevalence and risk factors associated with foot-and-mouth disease (FMD) seropositivity in north central, Nigeria. A cross-sectional study was undertaken from February 2013 to April 2014 using 1206 sera from 150 herds collected by multi-stage and random sampling methods. Pre-tested questionnaire were also administered to participating farmers to collect information on the...

Author(s): Wungak, Y. S., Olugasa, B. O., Ishola, O. O., Lazarus, D. D. and Ularamu, G. H.

June 2016

GhNAC18, a novel cotton (Gossypium hirsutum L.) NAC gene, is involved in leaf senescence and diverse stress responses

GhNAC18 is a novel NAC gene that was isolated from cotton (Gossypium hirsutum L.). The full-length cDNA was 1511 bp including an open reading frame of 1260 bp in length and encodes a protein of 419 amino acids. With qRT-PCR analysis, GhNAC18 was downregulated during natural and dark-induced senescence, implicating this gene as anti-aging gene in cotton. Analysis of its promoter revealed a group of putative cis-acting...

Author(s): Ondati Evans, Lingling Dou, Yaning Guo, Chaoyou Pang, Hengling Wei, Meizhen Song, Shuli Fan and Shuxun Yu

June 2016

Fatty acids and amino acids contents in Scomber scombrus fillets from the South East of Tunisia

Proximate composition, fats and amino acids of Atlantic Mackerel fillets (Scomber scombrus) from the South East of Tunisia in different seasons, were analyzed in order to assess nutritive characteristics of this species. Samples were collected monthly from Zarzis fishing port located in the South-East of Tunisia. Total fats and protein contents varied significantly (P<0.05) according to the season. The highest values...

Author(s): EL Oudiani Salma, Darej Cyrine and Moujahed Nizar

June 2016

Biochemical and hematological profile of different breeds of goat maintained under intensive production system

The aim of the study was to monitor the health and nutritional status of Kuwaiti’s Aradi and exotic Damascus and Barbari goat breeds raised under an intensive system of production through the determination of biochemical parameters and hematology profiles. The study was conducted during the summer season and blood samples were collected from lactating does and Damascus bucks. According to the findings of the...

Author(s): Samira Ahmed Mohammed, Mohammed Abdur Razzaque, Anaam E. Omar, Sheeba Albert, and Waleed M. Al-Gallaf

June 2016

Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeorohita): Extraction and characterization

Chitosan is a naturally available biopolymer. It has been prepared by alkaline N-deacetylation process of shrimp (Crangon crangon) chitin and fish (Labeorohita) chitin. The physico-chemical properties such as the degree of deacetylation (DD), solubility, water binding capacity, fat binding capacity and chitosan yield have indicated that shrimp shell and fish scale waste are good sources of chitosan. The deacetylation...

Author(s): Suneeta Kumari, Rath P. and Sri Hari Kumar A.

June 2016

In vitro regeneration of a common medicinal plant, Ocimum sanctum L. for mass propagation

Embracing micro-propagation method for large scale production of plantlets and also for protection of appropriate germplasm is a prerequisite that needs to be undertaken in order to develop a rapid in vitro regeneration protocol for Ocimum sanctum L. Shoot tips as well as nodal segments were subjected to numerous shoots inducement. Explants were cultured on Murashige and Skoog Basal Medium (MS) supplemented for...

Author(s): Mohammad Abu Hena Mostofa Jamal, Imdadul Hoque Sharif, Md. Mostofa Shakil, A. N. M. Rubaiyath-Bin Rahman, Nilufa Akhter Banu, Md. Rezuanul Islam and Md. Nazmuzzaman

June 2016

An improved plating assay for determination of phage titer

Phage is a virus that is parasitic on bacteria. It is very important to determine the titer of test sample in the study of phage. In this study, an improved plating assay was developed for detection of the number of recombinant phage Cap-T7 present in a test solution at a certain dilution point by counting the plaque forming units. The data demonstrated that the improved plating assay is fast, useful, and convenient for...

Author(s): Liu Yang, Chao Li, Shao-Qin Zhai, Hua Zheng, Li-Zhi Fu, and Da-Jun Li,

June 2016

Improving the optimum yield and growth of Chlamydomonas reinhardtii CC125 and CW15 using various carbon sources and growth regimes

Chlamydomonas reinhardtii CC125 (wild type) and CW15 (cell wall mutants) were feed up on solid and liquid Tris phosphate (TP) media with various concentrations of acetate, glycerol(10-100 mM) or methanol (0.01-718 mM) and cultivated under phototrophic, mixotrophic and heterotrophic conditions. Use of 10 and 35 mM acetate and 10 and 50 mM glycerol under constant 38 µE m−2 s−1 light illumination...

Author(s): Navid Taghavi and Gary Robinson

June 2016

Variation in levels and removal efficiency of heavy and trace metals from wastewater treatment plant effluents in Cape Town and Stellenbosch, South Africa

This study focused on one year monitoring campaign to monitor the occurrence and removal of Endocrine Disruptive Metals (EDMs) and trace metals from selected wastewater treatment plants (WWTPs) in Stellenbosch and Cape Town. Composite water samples were collected from the WWTPs from January 2010 to December 2010 on a quarterly basis and concentrations determined using inductively coupled plasma-mass spectrometry...

Author(s): Olujimi, O. O., Fatoki, O. S., Odendaal, J. P., Daso, A. P. and Oputu, O. U.

June 2016

Genetic diversity studies on selected rice varieties grown in Africa based on aroma, cooking and eating quality

Rice grain quality is an important factor that has a great influence on its market value and consumer acceptance. It is determined by three parameters controlling the cooking and eating qualities of rice (amylose content, gelatinization temperature and gel consistency) and by the aroma, which becomes a criterion increasingly preferred by consumers. Molecular characterization of specific genomic regions of rice genotypes...

Author(s): Koffi Kibalou Palanga, Karim Traore, Kofi Bimpong, Muhammad Jamshed and Mtawa A. P. Mkulama

June 2016

Genetic diversity and population structure among sorghum (Sorghum bicolor, L.) germplasm collections from Western Ethiopia

The Western Ethiopian region harbors a unique set of sorghum germplasm adapted to conditions not conventional to sorghums grown in other parts of the world. Accessions from the region possess unique resistance to multiple leaf and grain diseases. This study is aimed at exploring the extent of genetic variation and population structure among accessions of this region. A total of 123 accessions comprising 111 from Western...

Author(s): Dilooshi K. Weerasooriya, Frank R. Maulana, Ananda Y. Bandara, Alemu Tirfessa, Amsalu Ayana, Girma Mengistu, Kinde Nouh and Tesfaye T. Tesso

June 2016

Optimal concentration of selective agents for inhibiting in vitro growth of Urochloa brizantha embryogenic calli

The ability to distinguish transgenic cells of untransformed cell mass is a key step for the production of transgenic plants. Thus, the use of selection marker genes for identification of genetically modified plants is necessary. The aim of this study was to determine the optimal concentration of four selective agents (kanamycin, hygromycin, phosphinothricin and mannose) to inhibit in vitro growth of Urochloa brizantha...

Author(s): Alyne Valéria Carrion Pereira, Luiz Gonzaga Esteves Vieira and Alessandra Ferreira Ribas

June 2016

Bioactivity of mangrove humic materials on Rizophora mangle and Laguncularia racemosa seedlings, Brazil

The mangrove sediments are rich in organic matter and humic substances, responsible for important functions such as reducing the toxicity of heavy metals, nutrient stabilization, serving as drain to atmospheric carbon and increasing the plant growth. In this study, we observed the effect of humic substances, humic acids and fulvic acids isolated from sediments of a mangrove forest, sampled from the Municipal Ecological...

Author(s): Leonardo Barros Dobbss, André Luiz Paier Barroso, Alessandro Coutinho Ramos, Karla Stéphanie Nunes Torrico, Fabíola Schunk de Souza Arçari and Daniel Basílio Zandonadi

June 2016

Comparative assessment of different poultry manures and inorganic fertilizer on soil properties and nutrient uptake of maize (Zea mays L.)

This study investigated soil physicochemical properties and nutrient uptake of a drought-tolerant maize variety (DT-SR-WC2) in a derived savanna zone of Southwestern Nigeria when different composted poultry manures (CPM) and inorganic fertilizer (IF) were applied. This was with a view to determining the essential trace elements and nutritional quality of the test crop. The experiment was a randomized complete block...

Author(s): Moses B. Adewole, Olusegun M. Otitoju and Aderonke A. Okoya

June 2016

Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment

In recent years, intense interest has been drawn to the effects of radio-frequency electromagnetic field (RF-EMF) on reproduction. To explore the effects of RF-EMF on sperm maturation in epididymis microenvironment, 24 male Sprague Dawley (SD) rats were randomly divided into three exposed groups (SAR 1, 2 and 4 W/kg) and one sham group. The rats in exposed group were exposed to 1.84 GHz RF-EMF for 5 days (1 h/day)....

Author(s): Guangzhou An, Jing Li, Shenglong Xu, Tao Zhao, Kangchu Li, Jiajin Lin, Yan Zhou, Lihua Zeng, Guozhen Guo and Gui-Rong Ding

June 2016

Investigation of yeast genes possibly involved in mtDNA stability using the nematode Caenorhabditis elegans

Screening of Caenorhabditis elegans genes possibly involved in the mitochondrial genome maintenance was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to yeast genes known to be involved in mtDNA stability but of unknown molecular function or to identify transient components that could play important role on the stability of mtDNA...

Author(s): Addo M. G., Cossard R., Pichard D., Obiri-Danso K., Rötig A. and Delahodde A.

June 2016

Molecular characterization of potato (Solanum tuberosum L.) genotypes using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers

19 random amplified polymorphic DNA (RAPD) and five Inter-simple sequence repeat (ISSR) primers were used to characterize 35 potato accessions originating from Mexico, Europe and U.S.A, with the aim of generating information on the genetic diversity and relationship among the genotypes for better exploitation in breeding programs. A total of 222 and 82 loci were generated by the RAPD and ISSR primers, respectively. Both...

Author(s): Onamu R., Legaria J., Rodríguez J. L., Sahagùn J. and Pèrez J.

June 2016

In vitro regeneration of ‘Feizixiao’ litchi (Litchi chinensis Sonn.)

A simple efficient in vitro plant regeneration system was developed by indirect somatic embryogenesis of ‘Feizixiao’ litchi (Litchi chinensis Sonn.). Pollen in the anther of monocytes was used to induce callus. Two auxins (naphthalene acetic acid [NAA] and 2,4-dichloriphenoxyacetic acid [2,4-D]), and two cytokines (kinetin [KT] and 6-benzyladenine [BA]) were tested to explore their influence on callus...

Author(s): Guo Wang, Huanling Li, Shujun Wang, Jinhua Sun, Xinchun Zhang and Jiabao Wang

June 2016

Standardization of DNA extraction from invasive alien weed Parthenium hysterophorus

DNA isolation from the weed, Parthenium hysterophorus is complicated due to the presence of high amount of allelochemicals in the form of secondary metabolites that causes hindrance in extraction and enzymatic reactions. A modified and efficient DNA extraction from P. hysterophorus leaf has been developed. The present protocol is a modified version of cetyltrimethylammonium bromide (CTAB) method constituting high salt...

Author(s): Javed Ahmad, Mohamed M. Ibrahim, M. Affan Baig,  Arlene A. Ali and M. Irfan Qureshi

June 2016

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Medicinal properties of loquat leaf extracts (LLEs) are associated with their constituents of phenolic compounds and triterpenes. In this study, the efficacy of subcritical water extraction (SWE) technique was assessed by comparing with conventional solid-liquid extraction (CE) and Soxhlet extraction (SE). Results showed that the highest yields of total polyphenols were 82.7 ± 1.5 mgGAE/g leaf weight (LW), total...

Author(s): Erasto Mlyuka, Shuang Zhang, Zongping Zheng and Jie Chen,

June 2016

Nutritional profiles of tiger Nut (Cyperus esculentus) plant organs during its growth cycle

This study was carried out to determine major nutrient profiles changes of tiger nut plant during its growth period. The plant leaves, roots, tuber moisture, starch, fat and protein were analyzed by oven drying, enzymatic hydrolysis, glucose assay, soxhlet extraction and kjeldahl methods. The results show the moisture content was decreased during its growth cycle but varied with different plant organ. For leaves, the...

Author(s): Jing Si-qun#, Ebeydulla Rahman#, Zheng-hui Chen and Yao-xiang Wang

June 2016

Bioremediating silty soil contaminated by phenanthrene, pyrene, benz(a)anthracene, benzo(a)pyrene using Bacillus sp. and Pseudomonas sp.: Biosurfactant/Beta vulgaris agrowaste effects

Polycyclic aromatic hydrocarbons (PAHs) are recalcitrant contaminants which are routinely found in numerous environmental matrices, contributing to ecological degradation. In this study, the removal of LMW and HMW PAHs with 4- and 5-benzene rings, by Bacillus licheniformis STK 01, Bacillus subtilis STK 02 and Pseudomonas aeruginosa STK 03, was evaluated in silty soil for a period of 60 days. Subsequently, a...

Author(s): Olusola S. Amodu,, Tunde V. Ojumu and Seteno K. O. Ntwampe

June 2016

Temporal profiles of intra- and extracellular laccase isoenzymes from Pleurotus ostreatus produced in submerged and solid-state cultures

Laccases are enzymes that have a great potential for use in breaking down toxic compounds. Fungal laccases show high enzymatic activity, especially those produced by basidiomycetes. Depending on the culture conditions and the strain used, a variety of isoenzymes and/or enzymatic activities can be obtained. In this study, extracellular laccase enzymes produced by Pleurotus ostreatus was identified in a submerged culture...

Author(s): Anahí Armas-Tizapantzi, Elizet Cuatecontzi-Cuautle, Francisco J. Fernández, Arturo Estrada-Torres and Alba Mónica Montiel-González

May 2016

Effect of cadmium on the morphology and anatomy of Salvinia auriculata

This study aimed to evaluate the morphological and anatomical changes of Salvinia auriculata exposed to different concentrations 0, 2.5, 5, 7.5 and 10 µM of cadmium (Cd)  and its effect on plant growth. The experiment was conducted in the laboratory of Plant Anatomy of the IF Goiano/Rio Verde Campus, Goiás. Cd free samples of S. auriculata, was obtained from the Aquários Plantados company,...

Author(s): Rodrigues, D. A., Vasconcelos Filho, S. C, Rodrigues, A. A., Rampazzo, D. F., Rodrigues, C. L., Vasconcelos, J. M. and Magalhães, P. A. N. R.

May 2016

Surface composition and surface properties of water hyacinth (Eichhornia crassipes) root biomass: Effect of mineral acid and organic solvent treatment

The surface composition and surface properties of water hyacinth (Eichhornia crassipes) root biomass were studied before and after extraction with dilute nitric acid and toluene/ethanol (2/1, v/v) followed by ethanol, using Fourier Transform Infra-red (FT-IR) spectroscopy, thermogravimetric analysis, x-ray diffraction, scanning electron microscopy. FT-IR absorption bands were obtained at 3421, 2855, 1457 and 1035 cm-1...

Author(s): Netai Mukaratirwa-Muchanyereyi, , Jameson Kugara and Mark Fungayi Zaranyika

May 2016

In-silico smart library design to engineer a xylose-tolerant hexokinase variant

Saccharomyces cerevisiae has two hexokinases ScHxk1 and ScHxk2 that catalyze ATP-dependent phosphorylation of glucose and other hexoses. ScHxk2 plays an important role in glucose metabolism and the process of bioethanol production. The presence of xylose in the fermentation medium was found to inhibit ScHxk2. Therefore development of ScHxk2 variants that are resistant to the action of xylose is needed. In the current...

Author(s): Yasser Gaber

May 2016

Morphological diversity in oleaginous watermelon (Citrullus mucosospermus) from the Nangui Abrogoua University germplasm collection

A hundred and seventy-one oleaginous watermelon accessions either collected from different countries or obtained from gene banks were evaluated and compared based on 11 quantitative morphological traits. Principal component analysis on 11 traits revealed 81.19% of the total variability and pointed out variations among accessions, mainly on the basis of fruit size and weight. The dendrogram and factorial discriminant...

Author(s): Ahou A. Gbotto, Kouamé K. Koffi, Nandy D. Fouha Bi, Serge T. Doubi Bi, Hippolyte H. Tro, Jean-Pierre Baudoin and Irié A. Zoro Bi

May 2016

Impact of transgenic cotton expressing cry1Ac and cry2Ab genes on soil rhizosphere bacterial and fungal populations in soils of central Kenya

The impact of 9 months cultivation of transgenic Bacillus thuringiensis (Bt) cotton on selected culturable bacterial and fungal populations in rhizosphere soil was investigated. The transgenic Bt cotton line (06Z604M), isoline (99M03) and a non-Bt cotton line (HART 89M) were planted in confined field site in Samuru-Thika where non-Bt cotton had been continuously cultivated for the past 2 years. Rhizosphere...

Author(s): J. Swilla, C. N. Waturu and S. T. Rubindamayugi

May 2016

Expression and purification of recombinant Shiga toxin 2B from Escherichia coli O157:H7

Enterohemorrhagic Escherichia coli are important human food-borne pathogens. Recently, Shiga toxin-producing E. coli (STEC) causes life-threatening hemolytic-uremic syndrome (HUS). In this study, Stx2B gene, a subunit of Shiga toxin, was amplified via polymerase chain reaction (PCR) from the chromosomal DNA of clinical fecal sample using appropriate primers. The PCR product was cloned to commercially available plasmid...

Author(s): Marwa E. A. Aly,  Amro S. Hanora, Tamer M. Essam and Magdy A. Amin

May 2016

Genetic characterization by amplified fragment length polymorphism (AFLP) markers and morphochemical traits of Carica papaya L. genotypes

Carica papaya L. is a native fruit from Central America and Mexico and it is an economically important fruit. As a pre-breeding genetic study, the variability of both parents (L7 and M22) and the F1 individuals derived from their crosses (L7 × M22), was evaluated in terms of 32 morphochemical traits, and contrasted with their genetic diversity indicated by amplified fragment length polymorphism (AFLP) markers....

Author(s): Mariela Vázquez Calderón, Javier O. Mijangos-Cortés, Manuel J. Zavala L., L. Felipe Sánchez Teyer, Adriana Quiroz M., Matilde Margarita Ortiz G., Fernando Amilcar Contreras M., Francisco Espadas G., Gabriela Fuentes Ortiz and Jorge M. Santamaría

May 2016

Assessment of genetic diversity among sixty bread wheat (Triticum aestivum) cultivars using microsatellite markers

Assessment of genetic diversity among wheat cultivars is important to ensure that a continuous pool of cultivars with varying desirable traits is maintained. In view of this, a molecular study was conducted to assess the genetic diversity of sixty wheat cultivars using sixty microsatellite markers. Amplified alleles from each cultivar were scored after running in 6% poly acrylamide gel electrophoresis (PAGE). A...

Author(s): Vincent Mgoli Mwale, Xiuli Tang and Eric Chilembwe

May 2016

Fermentative intensity of L-lactic acid production using self-immobilized pelletized Rhizopus oryzae

L-Lactic acid is a promising three-carbon building-block chemical, widely used in the food, pharmaceutical, leather and textile industries and Rhizopus oryzae is an important filamentous fungus for the production of L-lactic acid with high optical purity. This study investigated the medium compositions for the maximum biomass cultivation of R. oryzae L-lactic acid fermentation, and optimized the operation parameters for...

Author(s): Shuizhong Luo, Xuefeng Wu, Yu Zhu, Xingjiang Li, Shaotong Jiang and Zhi Zheng

May 2016

Effect of pH, various divalent metal ion and different substrates on glucoamylase activity obtained from Aspergillus niger using amylopectin from tiger nut starch as carbon source

Glucoamylases were obtained from Aspergillus niger using amylopectin from tiger nut starch as carbon source on the 5th (GluAgTN5) and 12th day (GluAgTN12) of fermentation. The optimal pH for GluAgTN5 at 55°C were 6.5, 7.0, 6.0 while that for GluAgTN12 were 8.5, 6.0, 7.5 at 50°C using cassava, guinea corn and tiger nut starch as substrates, respectively. The enzyme activity in GluAgTN5 was enhanced by Ca2+ and...

Author(s): Ezugwu A. L., Ottah, V. E., Eze, S. O. O. and Chilaka F. C.

May 2016

Trials to improve the response of Orechromis niloticus to Aeromonas hydrophila vaccine using immunostimulants (garlic, Echinacea) and probiotics (Organic GreenTM and Vet-YeastTM)

This work aimed to investigate the role of some immunostimulants and probiotics in improving the response of overwintered tilapia to Aeromonas hydrophila vaccine. In this study, 15000 Nile tilapia fry (Orechromis niloticus) were collected and divided into five groups. Group 1 was the control, groups 2 to 5 were fed diet supplemented with garlic, Echinacea, Organic GreenTM and Vet-YeastTM respectively for 5 months....

Author(s): Salah Mesalhy Aly, Mohamed A. Al Zohairy, Arshad H. Rahmani, Mohamed Fathi and Nashwa M. Abdel Atti

May 2016

Fertigation with domestic wastewater: Uses and implications

The use of wastewater in agriculture is an alternative means of reducing wastewater release into water sources. This process, known as fertigation is an opportunity to make use of organic matter and other nutrients in wastewater for agricultural productivity. The presence of organic matter in these effluents serves as biofertilizer. Since 19th century in Britain, fertigation had been widely accepted due to the scarcity...

Author(s): José Geraldo Delvaux Silva, Joaquim José de Carvalho, José Maria Rodrigues da Luz and José Expedito Cavalcante da Silva

May 2016

Selection criteria for drought tolerance at the vegetative phase in early maturing maize

Identifying drought tolerant maize (Zea mays L.) at the vegetative stage is a meaningful effort at reducing cost and time of screening large number of maize genotypes for drought tolerance. The primary objectives of this study were to assess the effectiveness of vegetative traits in discriminating between drought tolerant and drought sensitive hybrids and to determine the stage at which the stress should be imposed to...

Author(s): R. O. Akinwale, M. A. B. Fakorede, A. Oluwaranti, B. Badu-Apraku and I. I. Adejumobi

May 2016

Isolation and molecular identification of yeast strains from “Rabilé” a starter of local fermented drink

“Rabilé” is dried yeast harvested from Sorghum beer, used as a traditional starter culture but more especially as ingredient in sauce and food cooking in Burkina Faso. The present study aimed to isolate and identify indigenous yeast flora of “Rabilé”. Standard microbiological process was carried out to value and isolate yeast in different samples of “Rabilé”...

Author(s): Ibrahim Keita, Marius K. Somda, Aly Savadogo, Iliassou Mogmenga, Ousmane Koita and Alfred S. Traore

May 2016

Genetic linkage map of cowpea (Vigna unguiculata (L.) Walp) using SNP markers

Genetic linkage maps provide a genomic framework for quantitative trait loci identification applied in marker assisted selection breeding in crops with limited resources. It serves as a powerful tool to breeders for analysing the mode of inheritance of genes of interest and monitoring of the transmission of target genes from parents to progeny. Single nucleotide polymorphism (SNP) markers have a great influence on...

Author(s): Adetumbi J. A., Akinyosoye S. T., Olowolafe M. O., Oloyede-Kamiyo Q. O. and Agbeleye O. A.

May 2016

Isomaltulose production using free and immobilized Serratia plymuthica cells

Isomaltulose is a low cariogenic sweetener used as a substitute for sucrose in the food industry. In this study, isomaltulose production by Serratia plymuthica ATCC 15928 was performed using free and immobilized cells. Response Surface Methodology was employed to evaluate the influence of temperature, wet cell mass concentration and sucrose concentration during the conversion of sucrose into isomaltulose by free cells....

Author(s): Daniela Castilho Orsi and Helia Harumi Sato

May 2016

The use of Amazon fungus (Trametes sp.) in the production of cellulase and xylanase

In order to use the residue from the beneficiation of Brazil nuts (Bertholletia excelsa HBK) as substrate in solid-sate fermentation (SSF), in this work, the production of cellulase and xylanase by the fungus Trametes sp. was investigated, using the residue as a carbon source. Employing a 24-1 fractional experimental design, the influence of substrate moisture, nutrient addition and inoculum quantity on enzymatic...

Author(s): Salony Aquino Pereira, Rafael Lopes e Oliveira, Sergio Duvoisin Jr., Leonor Alves de Oliveira da Silva and Patrícia Melchionna Albuquerque,

May 2016

Preliminary characterization of slow growing rhizobial strains isolated from Retama monosperma (L.) Boiss. root nodules from Northwest coast of Algeria

In this paper, we did some preliminary characterization of six slow growing rhizobial strains, isolated from Retama monosperma (L.) Boiss. root nodules sampled from 3 sites along the coast of Oran (CapeFalcon, Bousfer and MersElHadjadj) in Northwestern Algeria. Results of this study showed that all strains had a very slow growth rate in yeast malt (YM) agar medium, forming colonies less than 1 mm in diameter after seven...

Author(s): HANNANE Fatima Zohra, KACEM Mou,rad and KAID-HARCHE Meriem

May 2016

Sero-epidemiology of bluetongue in Algerian ruminants

A total of 2871 blood samples were collected (450 cattle, 1932 sheep and 489 goats) from 225 randomly sampled herds between January and June 2014. Competitive enzyme-linked immunosorbent assay (C-ELISA) was used to detect antibodies from sera as an indicator of exposure to bluetongue virus (BTV). The results show an overall herd seroprevalence of 16.44% (95% CI 9.42-23.46); 21.3% (95% CI 17.1-25.6) for cattle and 13.33%...

Author(s): Moustafa KARDJADJ, Pam Dachung LUKA and Meriem Hind BENMAHDI

May 2016

Role of arbuscular mycorrhizal fungi in phytoremediation of heavy metals and effects on growth and biochemical activities of wheat (Triticum aestivum L.) plants in Zn contaminated soils

The increase of metals pollution in soil is a worldwide problem that affects the health of humans and environment. The use of green technology such as phytoremediation is one of the environmental friendly techniques, in which plants and other microbes are used to reduce the level of metals contaminants in soil and lower its uptake towards plants tissues. Studies report that a number of cereal crops such as wheat...

Author(s): Sadia Kanwal, Asma Bano and Riffat Naseem Malik

May 2016

Physico chemical evaluation of coffee husk, wastes of enset (Enset ventricosum), vegetable and khat (Catha edulis) through vermicomposting employing an epigeic earthworm Dendrobaena veneta (Rosa, 1886)

The major objective of this 90 days vermicomposting work was to evaluate the performance of epigeic earthworms, Dendrobaena veneta, to alter and change four commonly dumped and littered solid wastes in Ethiopian cities and towns into a high quality vermicompost. The experiments were conducted in cylindrical plastic containers with 26 cm depth and 53 cm width under shade. All containers were perforated on the sides for...

Author(s): Gezahegn Degefe , Seyoum Mengistou and Said Mohammed

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