African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

AJB Articles

June 2016

Improving the optimum yield and growth of Chlamydomonas reinhardtii CC125 and CW15 using various carbon sources and growth regimes

Chlamydomonas reinhardtii CC125 (wild type) and CW15 (cell wall mutants) were feed up on solid and liquid Tris phosphate (TP) media with various concentrations of acetate, glycerol(10-100 mM) or methanol (0.01-718 mM) and cultivated under phototrophic, mixotrophic and heterotrophic conditions. Use of 10 and 35 mM acetate and 10 and 50 mM glycerol under constant 38 µE m−2 s−1 light illumination...

Author(s): Navid Taghavi and Gary Robinson

June 2016

Variation in levels and removal efficiency of heavy and trace metals from wastewater treatment plant effluents in Cape Town and Stellenbosch, South Africa

This study focused on one year monitoring campaign to monitor the occurrence and removal of Endocrine Disruptive Metals (EDMs) and trace metals from selected wastewater treatment plants (WWTPs) in Stellenbosch and Cape Town. Composite water samples were collected from the WWTPs from January 2010 to December 2010 on a quarterly basis and concentrations determined using inductively coupled plasma-mass spectrometry...

Author(s): Olujimi, O. O., Fatoki, O. S., Odendaal, J. P., Daso, A. P. and Oputu, O. U.

June 2016

Genetic diversity studies on selected rice varieties grown in Africa based on aroma, cooking and eating quality

Rice grain quality is an important factor that has a great influence on its market value and consumer acceptance. It is determined by three parameters controlling the cooking and eating qualities of rice (amylose content, gelatinization temperature and gel consistency) and by the aroma, which becomes a criterion increasingly preferred by consumers. Molecular characterization of specific genomic regions of rice genotypes...

Author(s): Koffi Kibalou Palanga, Karim Traore, Kofi Bimpong, Muhammad Jamshed and Mtawa A. P. Mkulama

June 2016

Genetic diversity and population structure among sorghum (Sorghum bicolor, L.) germplasm collections from Western Ethiopia

The Western Ethiopian region harbors a unique set of sorghum germplasm adapted to conditions not conventional to sorghums grown in other parts of the world. Accessions from the region possess unique resistance to multiple leaf and grain diseases. This study is aimed at exploring the extent of genetic variation and population structure among accessions of this region. A total of 123 accessions comprising 111 from Western...

Author(s): Dilooshi K. Weerasooriya, Frank R. Maulana, Ananda Y. Bandara, Alemu Tirfessa, Amsalu Ayana, Girma Mengistu, Kinde Nouh and Tesfaye T. Tesso

June 2016

Optimal concentration of selective agents for inhibiting in vitro growth of Urochloa brizantha embryogenic calli

The ability to distinguish transgenic cells of untransformed cell mass is a key step for the production of transgenic plants. Thus, the use of selection marker genes for identification of genetically modified plants is necessary. The aim of this study was to determine the optimal concentration of four selective agents (kanamycin, hygromycin, phosphinothricin and mannose) to inhibit in vitro growth of Urochloa brizantha...

Author(s): Alyne Valéria Carrion Pereira, Luiz Gonzaga Esteves Vieira and Alessandra Ferreira Ribas

June 2016

Bioactivity of mangrove humic materials on Rizophora mangle and Laguncularia racemosa seedlings, Brazil

The mangrove sediments are rich in organic matter and humic substances, responsible for important functions such as reducing the toxicity of heavy metals, nutrient stabilization, serving as drain to atmospheric carbon and increasing the plant growth. In this study, we observed the effect of humic substances, humic acids and fulvic acids isolated from sediments of a mangrove forest, sampled from the Municipal Ecological...

Author(s): Leonardo Barros Dobbss, André Luiz Paier Barroso, Alessandro Coutinho Ramos, Karla Stéphanie Nunes Torrico, Fabíola Schunk de Souza Arçari and Daniel Basílio Zandonadi

June 2016

Comparative assessment of different poultry manures and inorganic fertilizer on soil properties and nutrient uptake of maize (Zea mays L.)

This study investigated soil physicochemical properties and nutrient uptake of a drought-tolerant maize variety (DT-SR-WC2) in a derived savanna zone of Southwestern Nigeria when different composted poultry manures (CPM) and inorganic fertilizer (IF) were applied. This was with a view to determining the essential trace elements and nutritional quality of the test crop. The experiment was a randomized complete block...

Author(s): Moses B. Adewole, Olusegun M. Otitoju and Aderonke A. Okoya

June 2016

Effects of 1.84 GHz radio-frequency electromagnetic field on sperm maturation in epididymis micro-environment

In recent years, intense interest has been drawn to the effects of radio-frequency electromagnetic field (RF-EMF) on reproduction. To explore the effects of RF-EMF on sperm maturation in epididymis microenvironment, 24 male Sprague Dawley (SD) rats were randomly divided into three exposed groups (SAR 1, 2 and 4 W/kg) and one sham group. The rats in exposed group were exposed to 1.84 GHz RF-EMF for 5 days (1 h/day)....

Author(s): Guangzhou An, Jing Li, Shenglong Xu, Tao Zhao, Kangchu Li, Jiajin Lin, Yan Zhou, Lihua Zeng, Guozhen Guo and Gui-Rong Ding

June 2016

Investigation of yeast genes possibly involved in mtDNA stability using the nematode Caenorhabditis elegans

Screening of Caenorhabditis elegans genes possibly involved in the mitochondrial genome maintenance was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegans genes homologous to yeast genes known to be involved in mtDNA stability but of unknown molecular function or to identify transient components that could play important role on the stability of mtDNA...

Author(s): Addo M. G., Cossard R., Pichard D., Obiri-Danso K., Rötig A. and Delahodde A.

June 2016

Molecular characterization of potato (Solanum tuberosum L.) genotypes using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers

19 random amplified polymorphic DNA (RAPD) and five Inter-simple sequence repeat (ISSR) primers were used to characterize 35 potato accessions originating from Mexico, Europe and U.S.A, with the aim of generating information on the genetic diversity and relationship among the genotypes for better exploitation in breeding programs. A total of 222 and 82 loci were generated by the RAPD and ISSR primers, respectively. Both...

Author(s): Onamu R., Legaria J., Rodríguez J. L., Sahagùn J. and Pèrez J.

June 2016

In vitro regeneration of ‘Feizixiao’ litchi (Litchi chinensis Sonn.)

A simple efficient in vitro plant regeneration system was developed by indirect somatic embryogenesis of ‘Feizixiao’ litchi (Litchi chinensis Sonn.). Pollen in the anther of monocytes was used to induce callus. Two auxins (naphthalene acetic acid [NAA] and 2,4-dichloriphenoxyacetic acid [2,4-D]), and two cytokines (kinetin [KT] and 6-benzyladenine [BA]) were tested to explore their influence on callus...

Author(s): Guo Wang, Huanling Li, Shujun Wang, Jinhua Sun, Xinchun Zhang and Jiabao Wang

June 2016

Standardization of DNA extraction from invasive alien weed Parthenium hysterophorus

DNA isolation from the weed, Parthenium hysterophorus is complicated due to the presence of high amount of allelochemicals in the form of secondary metabolites that causes hindrance in extraction and enzymatic reactions. A modified and efficient DNA extraction from P. hysterophorus leaf has been developed. The present protocol is a modified version of cetyltrimethylammonium bromide (CTAB) method constituting high salt...

Author(s): Javed Ahmad, Mohamed M. Ibrahim, M. Affan Baig,  Arlene A. Ali and M. Irfan Qureshi

June 2016

Subcritical water extraction of bioactive compounds from dry loquat (Eriobotrya japonica) leaves and characterization of triterpenes in the extracts

Medicinal properties of loquat leaf extracts (LLEs) are associated with their constituents of phenolic compounds and triterpenes. In this study, the efficacy of subcritical water extraction (SWE) technique was assessed by comparing with conventional solid-liquid extraction (CE) and Soxhlet extraction (SE). Results showed that the highest yields of total polyphenols were 82.7 ± 1.5 mgGAE/g leaf weight (LW), total...

Author(s): Erasto Mlyuka, Shuang Zhang, Zongping Zheng and Jie Chen,

June 2016

Nutritional profiles of tiger Nut (Cyperus esculentus) plant organs during its growth cycle

This study was carried out to determine major nutrient profiles changes of tiger nut plant during its growth period. The plant leaves, roots, tuber moisture, starch, fat and protein were analyzed by oven drying, enzymatic hydrolysis, glucose assay, soxhlet extraction and kjeldahl methods. The results show the moisture content was decreased during its growth cycle but varied with different plant organ. For leaves, the...

Author(s): Jing Si-qun#, Ebeydulla Rahman#, Zheng-hui Chen and Yao-xiang Wang

June 2016

Bioremediating silty soil contaminated by phenanthrene, pyrene, benz(a)anthracene, benzo(a)pyrene using Bacillus sp. and Pseudomonas sp.: Biosurfactant/Beta vulgaris agrowaste effects

Polycyclic aromatic hydrocarbons (PAHs) are recalcitrant contaminants which are routinely found in numerous environmental matrices, contributing to ecological degradation. In this study, the removal of LMW and HMW PAHs with 4- and 5-benzene rings, by Bacillus licheniformis STK 01, Bacillus subtilis STK 02 and Pseudomonas aeruginosa STK 03, was evaluated in silty soil for a period of 60 days. Subsequently, a...

Author(s): Olusola S. Amodu,, Tunde V. Ojumu and Seteno K. O. Ntwampe

June 2016

Temporal profiles of intra- and extracellular laccase isoenzymes from Pleurotus ostreatus produced in submerged and solid-state cultures

Laccases are enzymes that have a great potential for use in breaking down toxic compounds. Fungal laccases show high enzymatic activity, especially those produced by basidiomycetes. Depending on the culture conditions and the strain used, a variety of isoenzymes and/or enzymatic activities can be obtained. In this study, extracellular laccase enzymes produced by Pleurotus ostreatus was identified in a submerged culture...

Author(s): Anahí Armas-Tizapantzi, Elizet Cuatecontzi-Cuautle, Francisco J. Fernández, Arturo Estrada-Torres and Alba Mónica Montiel-González

May 2016

Effect of cadmium on the morphology and anatomy of Salvinia auriculata

This study aimed to evaluate the morphological and anatomical changes of Salvinia auriculata exposed to different concentrations 0, 2.5, 5, 7.5 and 10 µM of cadmium (Cd)  and its effect on plant growth. The experiment was conducted in the laboratory of Plant Anatomy of the IF Goiano/Rio Verde Campus, Goiás. Cd free samples of S. auriculata, was obtained from the Aquários Plantados company,...

Author(s): Rodrigues, D. A., Vasconcelos Filho, S. C, Rodrigues, A. A., Rampazzo, D. F., Rodrigues, C. L., Vasconcelos, J. M. and Magalhães, P. A. N. R.

May 2016

Surface composition and surface properties of water hyacinth (Eichhornia crassipes) root biomass: Effect of mineral acid and organic solvent treatment

The surface composition and surface properties of water hyacinth (Eichhornia crassipes) root biomass were studied before and after extraction with dilute nitric acid and toluene/ethanol (2/1, v/v) followed by ethanol, using Fourier Transform Infra-red (FT-IR) spectroscopy, thermogravimetric analysis, x-ray diffraction, scanning electron microscopy. FT-IR absorption bands were obtained at 3421, 2855, 1457 and 1035 cm-1...

Author(s): Netai Mukaratirwa-Muchanyereyi, , Jameson Kugara and Mark Fungayi Zaranyika

May 2016

In-silico smart library design to engineer a xylose-tolerant hexokinase variant

Saccharomyces cerevisiae has two hexokinases ScHxk1 and ScHxk2 that catalyze ATP-dependent phosphorylation of glucose and other hexoses. ScHxk2 plays an important role in glucose metabolism and the process of bioethanol production. The presence of xylose in the fermentation medium was found to inhibit ScHxk2. Therefore development of ScHxk2 variants that are resistant to the action of xylose is needed. In the current...

Author(s): Yasser Gaber

May 2016

Morphological diversity in oleaginous watermelon (Citrullus mucosospermus) from the Nangui Abrogoua University germplasm collection

A hundred and seventy-one oleaginous watermelon accessions either collected from different countries or obtained from gene banks were evaluated and compared based on 11 quantitative morphological traits. Principal component analysis on 11 traits revealed 81.19% of the total variability and pointed out variations among accessions, mainly on the basis of fruit size and weight. The dendrogram and factorial discriminant...

Author(s): Ahou A. Gbotto, Kouamé K. Koffi, Nandy D. Fouha Bi, Serge T. Doubi Bi, Hippolyte H. Tro, Jean-Pierre Baudoin and Irié A. Zoro Bi

May 2016

Impact of transgenic cotton expressing cry1Ac and cry2Ab genes on soil rhizosphere bacterial and fungal populations in soils of central Kenya

The impact of 9 months cultivation of transgenic Bacillus thuringiensis (Bt) cotton on selected culturable bacterial and fungal populations in rhizosphere soil was investigated. The transgenic Bt cotton line (06Z604M), isoline (99M03) and a non-Bt cotton line (HART 89M) were planted in confined field site in Samuru-Thika where non-Bt cotton had been continuously cultivated for the past 2 years. Rhizosphere...

Author(s): J. Swilla, C. N. Waturu and S. T. Rubindamayugi

May 2016

Expression and purification of recombinant Shiga toxin 2B from Escherichia coli O157:H7

Enterohemorrhagic Escherichia coli are important human food-borne pathogens. Recently, Shiga toxin-producing E. coli (STEC) causes life-threatening hemolytic-uremic syndrome (HUS). In this study, Stx2B gene, a subunit of Shiga toxin, was amplified via polymerase chain reaction (PCR) from the chromosomal DNA of clinical fecal sample using appropriate primers. The PCR product was cloned to commercially available plasmid...

Author(s): Marwa E. A. Aly,  Amro S. Hanora, Tamer M. Essam and Magdy A. Amin

May 2016

Genetic characterization by amplified fragment length polymorphism (AFLP) markers and morphochemical traits of Carica papaya L. genotypes

Carica papaya L. is a native fruit from Central America and Mexico and it is an economically important fruit. As a pre-breeding genetic study, the variability of both parents (L7 and M22) and the F1 individuals derived from their crosses (L7 × M22), was evaluated in terms of 32 morphochemical traits, and contrasted with their genetic diversity indicated by amplified fragment length polymorphism (AFLP) markers....

Author(s): Mariela Vázquez Calderón, Javier O. Mijangos-Cortés, Manuel J. Zavala L., L. Felipe Sánchez Teyer, Adriana Quiroz M., Matilde Margarita Ortiz G., Fernando Amilcar Contreras M., Francisco Espadas G., Gabriela Fuentes Ortiz and Jorge M. Santamaría

May 2016

Assessment of genetic diversity among sixty bread wheat (Triticum aestivum) cultivars using microsatellite markers

Assessment of genetic diversity among wheat cultivars is important to ensure that a continuous pool of cultivars with varying desirable traits is maintained. In view of this, a molecular study was conducted to assess the genetic diversity of sixty wheat cultivars using sixty microsatellite markers. Amplified alleles from each cultivar were scored after running in 6% poly acrylamide gel electrophoresis (PAGE). A...

Author(s): Vincent Mgoli Mwale, Xiuli Tang and Eric Chilembwe

May 2016

Fermentative intensity of L-lactic acid production using self-immobilized pelletized Rhizopus oryzae

L-Lactic acid is a promising three-carbon building-block chemical, widely used in the food, pharmaceutical, leather and textile industries and Rhizopus oryzae is an important filamentous fungus for the production of L-lactic acid with high optical purity. This study investigated the medium compositions for the maximum biomass cultivation of R. oryzae L-lactic acid fermentation, and optimized the operation parameters for...

Author(s): Shuizhong Luo, Xuefeng Wu, Yu Zhu, Xingjiang Li, Shaotong Jiang and Zhi Zheng

May 2016

Effect of pH, various divalent metal ion and different substrates on glucoamylase activity obtained from Aspergillus niger using amylopectin from tiger nut starch as carbon source

Glucoamylases were obtained from Aspergillus niger using amylopectin from tiger nut starch as carbon source on the 5th (GluAgTN5) and 12th day (GluAgTN12) of fermentation. The optimal pH for GluAgTN5 at 55°C were 6.5, 7.0, 6.0 while that for GluAgTN12 were 8.5, 6.0, 7.5 at 50°C using cassava, guinea corn and tiger nut starch as substrates, respectively. The enzyme activity in GluAgTN5 was enhanced by Ca2+ and...

Author(s): Ezugwu A. L., Ottah, V. E., Eze, S. O. O. and Chilaka F. C.

May 2016

Trials to improve the response of Orechromis niloticus to Aeromonas hydrophila vaccine using immunostimulants (garlic, Echinacea) and probiotics (Organic GreenTM and Vet-YeastTM)

This work aimed to investigate the role of some immunostimulants and probiotics in improving the response of overwintered tilapia to Aeromonas hydrophila vaccine. In this study, 15000 Nile tilapia fry (Orechromis niloticus) were collected and divided into five groups. Group 1 was the control, groups 2 to 5 were fed diet supplemented with garlic, Echinacea, Organic GreenTM and Vet-YeastTM respectively for 5 months....

Author(s): Salah Mesalhy Aly, Mohamed A. Al Zohairy, Arshad H. Rahmani, Mohamed Fathi and Nashwa M. Abdel Atti

May 2016

Fertigation with domestic wastewater: Uses and implications

The use of wastewater in agriculture is an alternative means of reducing wastewater release into water sources. This process, known as fertigation is an opportunity to make use of organic matter and other nutrients in wastewater for agricultural productivity. The presence of organic matter in these effluents serves as biofertilizer. Since 19th century in Britain, fertigation had been widely accepted due to the scarcity...

Author(s): José Geraldo Delvaux Silva, Joaquim José de Carvalho, José Maria Rodrigues da Luz and José Expedito Cavalcante da Silva

May 2016

Selection criteria for drought tolerance at the vegetative phase in early maturing maize

Identifying drought tolerant maize (Zea mays L.) at the vegetative stage is a meaningful effort at reducing cost and time of screening large number of maize genotypes for drought tolerance. The primary objectives of this study were to assess the effectiveness of vegetative traits in discriminating between drought tolerant and drought sensitive hybrids and to determine the stage at which the stress should be imposed to...

Author(s): R. O. Akinwale, M. A. B. Fakorede, A. Oluwaranti, B. Badu-Apraku and I. I. Adejumobi

May 2016

Isolation and molecular identification of yeast strains from “Rabilé” a starter of local fermented drink

“Rabilé” is dried yeast harvested from Sorghum beer, used as a traditional starter culture but more especially as ingredient in sauce and food cooking in Burkina Faso. The present study aimed to isolate and identify indigenous yeast flora of “Rabilé”. Standard microbiological process was carried out to value and isolate yeast in different samples of “Rabilé”...

Author(s): Ibrahim Keita, Marius K. Somda, Aly Savadogo, Iliassou Mogmenga, Ousmane Koita and Alfred S. Traore

May 2016

Genetic linkage map of cowpea (Vigna unguiculata (L.) Walp) using SNP markers

Genetic linkage maps provide a genomic framework for quantitative trait loci identification applied in marker assisted selection breeding in crops with limited resources. It serves as a powerful tool to breeders for analysing the mode of inheritance of genes of interest and monitoring of the transmission of target genes from parents to progeny. Single nucleotide polymorphism (SNP) markers have a great influence on...

Author(s): Adetumbi J. A., Akinyosoye S. T., Olowolafe M. O., Oloyede-Kamiyo Q. O. and Agbeleye O. A.

May 2016

Isomaltulose production using free and immobilized Serratia plymuthica cells

Isomaltulose is a low cariogenic sweetener used as a substitute for sucrose in the food industry. In this study, isomaltulose production by Serratia plymuthica ATCC 15928 was performed using free and immobilized cells. Response Surface Methodology was employed to evaluate the influence of temperature, wet cell mass concentration and sucrose concentration during the conversion of sucrose into isomaltulose by free cells....

Author(s): Daniela Castilho Orsi and Helia Harumi Sato

May 2016

The use of Amazon fungus (Trametes sp.) in the production of cellulase and xylanase

In order to use the residue from the beneficiation of Brazil nuts (Bertholletia excelsa HBK) as substrate in solid-sate fermentation (SSF), in this work, the production of cellulase and xylanase by the fungus Trametes sp. was investigated, using the residue as a carbon source. Employing a 24-1 fractional experimental design, the influence of substrate moisture, nutrient addition and inoculum quantity on enzymatic...

Author(s): Salony Aquino Pereira, Rafael Lopes e Oliveira, Sergio Duvoisin Jr., Leonor Alves de Oliveira da Silva and Patrícia Melchionna Albuquerque,

May 2016

Preliminary characterization of slow growing rhizobial strains isolated from Retama monosperma (L.) Boiss. root nodules from Northwest coast of Algeria

In this paper, we did some preliminary characterization of six slow growing rhizobial strains, isolated from Retama monosperma (L.) Boiss. root nodules sampled from 3 sites along the coast of Oran (CapeFalcon, Bousfer and MersElHadjadj) in Northwestern Algeria. Results of this study showed that all strains had a very slow growth rate in yeast malt (YM) agar medium, forming colonies less than 1 mm in diameter after seven...

Author(s): HANNANE Fatima Zohra, KACEM Mou,rad and KAID-HARCHE Meriem

May 2016

Sero-epidemiology of bluetongue in Algerian ruminants

A total of 2871 blood samples were collected (450 cattle, 1932 sheep and 489 goats) from 225 randomly sampled herds between January and June 2014. Competitive enzyme-linked immunosorbent assay (C-ELISA) was used to detect antibodies from sera as an indicator of exposure to bluetongue virus (BTV). The results show an overall herd seroprevalence of 16.44% (95% CI 9.42-23.46); 21.3% (95% CI 17.1-25.6) for cattle and 13.33%...

Author(s): Moustafa KARDJADJ, Pam Dachung LUKA and Meriem Hind BENMAHDI

May 2016

Role of arbuscular mycorrhizal fungi in phytoremediation of heavy metals and effects on growth and biochemical activities of wheat (Triticum aestivum L.) plants in Zn contaminated soils

The increase of metals pollution in soil is a worldwide problem that affects the health of humans and environment. The use of green technology such as phytoremediation is one of the environmental friendly techniques, in which plants and other microbes are used to reduce the level of metals contaminants in soil and lower its uptake towards plants tissues. Studies report that a number of cereal crops such as wheat...

Author(s): Sadia Kanwal, Asma Bano and Riffat Naseem Malik

May 2016

Physico chemical evaluation of coffee husk, wastes of enset (Enset ventricosum), vegetable and khat (Catha edulis) through vermicomposting employing an epigeic earthworm Dendrobaena veneta (Rosa, 1886)

The major objective of this 90 days vermicomposting work was to evaluate the performance of epigeic earthworms, Dendrobaena veneta, to alter and change four commonly dumped and littered solid wastes in Ethiopian cities and towns into a high quality vermicompost. The experiments were conducted in cylindrical plastic containers with 26 cm depth and 53 cm width under shade. All containers were perforated on the sides for...

Author(s): Gezahegn Degefe , Seyoum Mengistou and Said Mohammed

May 2016

An efficient protocol for in vitro organogenesis and antioxidant studies in Melia dubia Cav.

Melia dubia Cav. (Meliaceae) is a multipurpose tree of tropical and subtropical regions mainly cultivated for its medicinal and industrial importance. Due to its versatile properties, it has been depleted in its natural environment. Moreover due to sluggish and poor seed germination, there is a threat of its gene pool exclusion from the natural habitat. The alternative method for conservation and efficient mass...

Author(s): Syed Naseer Shah, Tareq Ahmad Wani , Bhimi Ram, Monika Koul, Praveen Awasthi, Deependra Singh Rajput and Gillela Ravi Shanker Reddy

May 2016

A novel pig feed formulation containing Aspergillus niger CSA35 pretreated-cassava peels and its effect on growth and selected biochemical parameters of pigs

This study investigated the effects of Aspergillus niger CSA35 pretreated-cassava (Manihot esculenta Crantz) peel feed (CPFG) on the body weight gain and some selected biochemical parameters of pigs. Cassava peels treated with biomass of A. niger CSA35 for a period of three weeks to initiate enzymatic digestion of peels were dried, ground and used in varying proportions to formulate pig rations in combination with other...

Author(s): Nyerhovwo J. Tonukari, Egbune E. Oliseneku, Oghenetega J. Avwioroko, Eferhire Aganbi, Osuvwe C. Orororo and Akpovwehwee A. Anigboro

May 2016

Inhibition of cell death as an approach for development of transgenic resistance against Fusarium wilt disease

Fusarium oxysporum f. sp. cubense (Foc) is one of the major threats to dessert banana (Musa spp.) production. In Uganda, ‘Sukali Ndiizi’ is one of the most popular dessert banana cultivars and it is highly susceptible to Fusarium wilt. Development of resistant cultivars through transgenic approaches has shown to offer one of the most effective control options for most diseases. The transgenic approaches for...

Author(s): Betty Magambo, Khanna Harjeet, Geofrey Arinaitwe, Sali Tendo Ivan Kabiita Arinaitwe, Jerome Kubiriba, Wilberforce Tushemereirwe and James Dale

May 2016

Evaluation of potential bio-control agents on root-knot nematode Meloidogyne incognita and wilt causing fungus Fusarium oxysporum f.sp. conglutinans in vitro

Indigenous strains of Trichoderma viride (ITCC No. 6889), Pseudomonas fluorescens (ITCC No. B0034) and Purpureocillium lilacinum (ITCC No.6887) were isolated from undisturbed forest eco-system of Southern India. These three bio-mediators were evaluated for their antagonism towards root knot nematode, Meloidogyne incognita and Fusarium oxysporum f.sp. conglutinans in vitro. Cell free culture filtrate of these strains...

Author(s): Rajinikanth Rompalli, Sreenivasa Rao Mehendrakar and Pavani Kantabathini Venkata

May 2016

Leaf anatomy of Crambe abyssinica Hochst. during in vitro shoot induction

This study aimed to characterize and evaluate possible modifications in the leaf anatomy of crambe during the process of shoot induction based on micropropagation protocol. The anatomic characteristics of the leaves, and also the morphological characteristics of crambe plantlets, were evaluated during the shoot induction phase of the micropropagation. The shoots were induced by the cytokinins, 6-benzylaminopurine (BAP),...

Author(s): Elias Terra Werner, Camilla Rozindo Dias Milanez, Andreia Barcelos Passos Lima Gontijo, Taís Cristina Bastos Soares and Jose Augusto Teixeira do Amaral

May 2016

Characterization of biochemical behavior of sorghum (Sorghum bicolor [Moench.]) under saline stress conditions using multivariate analysis

The aim of this research was to characterize the biochemical behavior of sorghum plants under saline stress using multivariate statistical analysis methods for efficient management of Sorghum bicolor [Moench.]). The experimental design was completely randomized design composed of three saline concentrations (0, 1.5 and 2.0 M) in 10 replications. In the multivariate analysis (hierarchical method), there were distinct and...

Author(s): Luma Castro de Souza, Mara Regina Moitinho, Risely Ferraz de Almeida, Ellen Gleyce da Silva Lima, Leane Castro de Souza, Myriam Galvão Neves, Cândido Ferreira de Oliveira Neto, Glauco André dos Santos Nogueira, Maria Eunice Lima Rocha, Mayra Taniely Ribeiro Abade and Marlison Tavares Ávila

May 2016

Short-term phyto-toxicity consequences of a non-selective herbicide glyphosate (Roundup™) on the growth of onions (Allium cepa Linn.)

This study examined the phyto-toxic effects of a commonly named non-selective herbicide glyphosate (Roundup™) on onions (Allium cepa Linn.). The study was necessitated due to the indiscriminate use and release of Roundup™ for weed control in the Niger Delta soils of Nigeria. The Organisation for Economic Co-operation and Development (OECD) standard protocol # 208 was adopted. The short-term phyto-toxic...

Author(s): D. F. Ogeleka, F. E. Okieimen, F. O. Ekpudi and L. E. Tudararo-Aherobo

May 2016

Morphological and molecular genetic diversity of Syrian indigenous goat populations

Domestic goats in Syria may provide an interesting source of genetic variability due to its proximity to the centers of domestication. This study aimed to assess the morphological variation, genetic diversity and population substructure of the Syrian goat populations. Commonly, three goat genotypes are distinguished in Syria, namely Jabali or mountain goat, Baladi or local goat and Shami or Damascus (a well-known dairy...

Author(s): Halima Hassen, Barbara Rischkowsky, Adnan Termanini, Ghassen Jessry, Aynalem Haile, Michael Baum and Samir Lababidi

May 2016

Lipase-producing fungi for potential wastewater treatment and bioenergy production

The use of fungal biomass as a lipase biocatalyst represents an attractive approach for the treatments of oil wastewater as well as for the production of biodiesel from oil and residual grease, due to its greater stability, possibility of reuse, and lower cost. In this work, 20 filamentous fungi were isolated from the grease trap scum of a restaurant at the Federal University of Espírito Santo, Brazil. The fungi...

Author(s): Celson Rodrigues, Sérvio Túlio Alves Cassini, Paulo Wagner Pereira Antunes, Laura Marina Pinotti, Regina de Pinho Keller and Ricardo Franci Gonçalves

April 2016

Cloning, over-expression, and characterization of a new carboxypeptidase A gene of Bacillus pumilus ML413 in Bacillus subtilis 168

Carboxypeptidase A (CPAs) are a well-studied group of zinc-containing exopeptidases that facilitate the breakdown of proteins and peptides during metabolism. Carboxypeptidase A is typically produced in mammalian pancreatic, brain and other tissues. A new gene encoding carboxypeptidase A in the prokaryote Bacillus pumilus was amplified by polymerase chain reaction (PCR), ligated into the shuttle vector pMA5, and cloned...

Author(s): TRAORE Tahirou, Junping zhou, Zhiming Rao, Genchu Huang, Taowei Yang, Xian Zhang and Meijuan Xu

April 2016

Production, optimization and characterization of extracellular amylase from halophilic Bacillus lichineformis AH214

Twenty one moderately halophilic bacterial strains were isolated from seawater and sediment in Alexandria Eastern Harbour, Egypt. The isolates were screened for the production of four extracellular degradative enzymes. The majority of isolates (57.1%) possessed significant enzyme activities, 43% of them have potentiality to produce amylase enzyme. The most active isolate for the production of amylase enzyme was...

Author(s): Hanan M. Abel-Nabey and Aida M. Farag

April 2016

Molecular serotype and evolutionary lineage of Listeria monocytogenes isolated from different Nigerian food items

The molecular serotypes and the evolutionary lineage of Listeria monocytogenes isolated from various foods in Nigeria are yet to be documented. Consequently, popular uncooked food items known locally as Okazi Utazi, Onugbu, Ogbono, Garri and Egusi obtained from plants botanically known as Gnetum africanum, Gongronema latifolium, Vernonia amygdalina, Irvingia gabonensis, Manihot esculanta Crantz and Colocynthis...

Author(s): Ogueri Nwaiwu,

April 2016

In vitro production of thiophenes using hairy root cultures of Tagetes erecta (L.)

Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous compounds possessing strong biocidal activity, thus making Tagetes plants very useful as natural source of agents for controlling pathogens such as nematodes, insects, fungi and bacteria. Hairy root cultures of Tagetes erecta L. were developed using Agrobacterium rhizogenes mediated transformation. The hairy root clones showed...

Author(s): Vijayta Gupta, Karuna Shanker and Laiq ur Rahman

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